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All Journal Jurnal Harpodon Borneo Jurnal Medik Veteriner Samakia: Jurnal Ilmu Perikanan Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Vitek : Bidang Kedokteran Hewan Jurnal Ilmiah Fillia Cendekia JURNAL KAJIAN VETERINER Prosiding Seminar Nasional Kusuma
Sheila Marty Yanestria
Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Veterinary Other

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Ekstrak Daun Salam (Eugenia polyantha, Weight.) sebagai Pengawet Alami pada Ikan Bandeng (Chanos chanos) Sheila Marty Yanestria; Asih Rahayu; Bunna Chrystin Rambu Uru; Adhitya Yoppy Ro Chandra
Samakia : Jurnal Ilmu Perikanan Vol 11 No 2 (2020): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.696 KB) | DOI: 10.35316/jsapi.v11i2.890

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak daun salam sebagai pengawet alami pada ikan bandeng di tambak Sidoarjo. Parameter pada penelitian ini adalah organoleptik, pH dan total koloni bakteri pada ikan bandeng. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 5 perlakuan dengan jumlah ulangan sebanyak 5 kali. Kelompok perlakuan P0 (tanpa perlakuan) sebagai kontrol, P1 (ekstrak daun salam 5%), P2 (ekstrak daun salam 10%), P3 (ekstrak daun salam 15%) dan P4 (ekstrak daun salam 20%). Hasil analisis statistik uji Total Koloni Bakteri serta uji pH menunjukkan perbedaan yang nyata (p<0,05), begitu pula dengan hasil analisis statistik uji organoleptik yang menunjukkan perbedaan nyata tiap perlakuan. Berdasarkan hasil data yang ada, disimpulkan bahwa terdapat pengaruh ekstrak daun salam sebagai pengawet alami pada ikan bandeng terutama pada konsentrasi daun salam sebesar 20%.
Tingkat Cemaran Escherichia Coli pada Susu Segar dari Peternakan Sapi Perah di Surabaya Sheila Marty Yanestria
VITEK : Bidang Kedokteran Hewan Vol 5 (2015): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The quality of milk is important to supply healthy milk for consumers. The increase of bacteria in fresh milk is one factor that can decrease the quality of milk. Bacteria in fresh milk can harm the health of consumers if the milk is not processed properly. Escherichia coli is a bacteria that often contaminate fresh milk. Escherichia coli can be pathogenic in humans because of some serotypes of this bacteria is able to produce the toxin. This toxin can cause fluid accumulation in the intestine and diarrhea. The purpose of this study was to determine the contaminant levels of Escherichia coli in fresh milk from dairy farms in Surabaya. The method used to determine the contaminant levels of Escherichia coli is Most Probable Number (MPN). The results showed that the average of Escherichia coli contaminant levels in fresh milk from dairy farms in Surabaya is 110 Escherichia coli / ml. This is not appropiate with the requirements of ISO 7388: 2009 (<3 Escherichia coli / ml).
Nilai pH dan deteksi Salmonella sp. Daging sapi di pasar tradisional dan pasar modern di wilayah surabaya timur Sheila Marty Yanestria; Asih Rahayu; Atina Atina
VITEK : Bidang Kedokteran Hewan Vol 11 No 1 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i1.72

Abstract

The purpose of this study was to determine the pH value and detection of Salmonella sp beef in traditional markets and modern markets in East Surabaya. This type of observational research with descriptive analysis based on the range of normal pH values ​​of beef and the biochemical properties of Salmonella sp. The samples used consisted of the following: traditional markets with codes A, B, C, D, and E, and modern markets with codes 1, 2, 3, 4, and 5. Each sample taken from each market consisted of cooked meat. deep or tenderloin with 2 different pieces. Based on the results of the study showed that there was no significant difference in the pH value of beef from traditional markets and modern markets in East Surabaya (P <0.05) and there was 1 positive meat sample for Salmonella sp from traditional markets and 2 meat samples. Positive Salmonella sp which comes from the modern market.
Deteksi Kasus Fasciolosis dan Eurytrematosis pada Pemeriksaan Antemortem dan Postmortem Hewan Qurban Saat Masa Pandemi Covid 19 di Surabaya Desty Apritya; Sheila Marty Yanestria; Intan Permatasari Hermawan
Jurnal Ilmiah Fillia Cendekia Vol 6 No 1 (2021): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v6i1.1197

Abstract

Kegiatan pengabdian masyarakat ini bertujuan untuk membantu masyarakat menjamin daging Qurban yang ASUH (Aman Sehat Utuh dan Halal) dengan pemeriksaan antemortem dan postmortem hewan Qurban di bawah naungan dokter hewan. Sasaran pengabdian adalah pengurus masjid atau masyarakat yang menyediakan dan mendistribusikan daging kurban di Surabaya dan sekitarnya. Metode pada kegiatan ini dilaksanakan pemeriksaan antemortem dan postmortem di 15 masjid, data dikoleksi dan kemudian dianalisis. Pemeriksaan ante mortem meliputi pemeriksaan fisik kesehatan hewan. Pemeriksaan post mortem meliputi pemeriksaan organ dalam (usus, lambung, hati, jantung, limpa, paru-paru) serta karkas. Tim pemeriksa melakukan prosedur protokol kesehatan pencegahan Covid 19 dalam menjalankan pemeriksaan . Total hewan qurban yang diperiksa sebanyak 116 ekor sapi, 214 ekor kambing dan 4 domba. Hasil pemeriksaan antemortem hewan qurban, 100% dinyatakan layak dan sesuai syariat islam baik dari segi umur dan kesehatan hewan. Hasil pemeriksaan postmortem ditemukan 27 (76%) hati sapi terinfeksi cacing Fasciola gigantica dan 2 (23 %) pankreas sapi terinfeksi Eurytrema sp, sedangkan pada kambing ditemukan 2 (0,9 %) hati terinfeksi cacing Fasciola gigantica. Organ sapi dan kambing yang terinfeksi cacing telah dilakukan afkir atau dimusnahkan sehingga tidak sampai terkonsumsi oleh masyarakat.
Antibakteri Nano Silver Terhadap Bakteri Escherichia coli Yang Diisolasi Dari Susu Sapi Mastitis Sheila Marty Yanestria; Era Hari Mudji; Elsa Rosita; Reina Puspita Rahmaniar
Jurnal Ilmiah Fillia Cendekia Vol 5 No 2 (2020): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v5i2.1178

Abstract

Penelitian ini dilakukan untuk mengetahui efektivitas antibakteri nano silver pada bakteri Escherichia coli yang diisolasi dari susu sapi mastitis. Penelitian ini menggunakan 5 perlakuan dengan 5 kali ulangan. Pada kelompok P0- sebagai kontrol negatif menggunakan Aquades, kelompok P0+ sebagai kontrol positif dengan perlakuan Amoxicillin, kelompok P1: perlakuan dengan larutan nano silver konsentrasi 50 ppm, kelompok P2: perlakuan dengan larutan nano silver konsentrasi 100 ppm dan kelompok P3: perlakuan dengan larutan nano silver dengan konsentrasi 150 ppm. Pada penelitian ini, pengamatan dilakukan dengan mengukur diameter zona hambat pada media MHA (Muller Hinton Agar) yang sudah dibiakkan bakteri murni Escherichia coli. Data yang diperoleh berdasarkan analisis menggunakan ANOVA menunjukan perbedaan yang sangat signifikan (P<0,05) pada setiap kelompok perlakuan. Hasil penelitian menunjukkan nano silver memiliki sifat antibakteri yang lemah terhadap bakteri Escherichia coli yang diisolasi dari susu sapi mastitis.
GEN RESISTEN ANTIBIOTIK PADA SALMONELLA YANG DIISOLASI DARI IKAN BANDENG (Chanos chanos) DI KABUPATEN SIDOARJO Sheila Marty Yanestria
Jurnal Ilmiah Fillia Cendekia Vol 5 No 1 (2020): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.639 KB) | DOI: 10.32503/fillia.v5i1.950

Abstract

Tujuan dari penelitian ini adalah untuk mengidentifikasi gen Tet(A), gen StrA dan gen Bla(tem) pada Salmonella yang diisolasi dari ikan bandeng (Chanos chanos) di Kabupaten Sidoarjo. Ada 20 sampel yang diambil dan diinokulasikan pada media pengayaan dan diisolasi pada permukaan Salmonella Shigella Agar. Salmonella memiliki bentuk koloni transparan dengan warna kehitaman di tengah karena pembentukan gas H2S. Sampel diidentifikasi sebagai Salmonella berdasarkan bentuk makroskopis dan morfologi koloni, pemeriksaan mikroskopis dengan pewarnaan gram. Hasil penelitian ini menunjukkan bahwa 9 dari 20 sampel adalah bakteri dari genus Salmonella dan 2 sampel memiliki gen invA. Selanjutnya,deteksi gen resisten menggunakan teknik Polymerase Chain Reaction. Elektroforesis hasil dari 2 sampel positif Salmonella patogen menunjukkan bahwa ada satu sampel positif gen tet(A), yang menghasilkan produk PCR dalam bentuk band dengan panjang 210 bp. Adanya gen resistensi antibiotik pada bakteri mengakibatkan pentingnya memantau kerentanan antimikroba dan mekanisme resistensi Salmonella yang diisolasi dari makanan, karena mekanisme baru dari resistensi yang terjadi pada hewan dapat memasuki rantai makanan dan ditransfer ke manusia. Kerjasama antar sektor sangat penting untuk memantau resistensi antimikroba di Indonesia.
Uji Kualitas (organoleptis, eber ) dan identifikasi cemaran Salmonella Sp. pada daging ayam dari pasar tradisional di Surabaya Barat: Quality test (organoleptics, eber) and identification of contaminants Salmonella Sp. on chicken from traditional markets in West Surabaya Freshinta Jellia Wibisono; Adhitya Yoppy Ro Candra; Mohammad Exceltyanto Widodo; Arief Mardijanto; Sheila Marty Yanestria
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 12 No. 1 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v12i1.252

Abstract

Abstract  Consumption of meat is one of the efforts to fulfill protein, because protein in meat (animal protein) is more complete than protein in plants (vegetable protein). Chicken meat is consumed by more people in Indonesia than beef, because it has a more affordable price and still has high nutritional value. The high nutritional content of meat makes it an agent for the development of infectious microbes that allow food poisoning to occur. Salmonella is one of the bacteria that causes food poisoning. Infection with this bacterium in animals or humans can cause illness with disorders of the digestive tract or gastroenteritis such as stomach cramps and diarrhea that lasts four to seven days. This study used 37 samples of chicken breast which were taken from several traditional markets in West Surabaya, and analyzed descriptively. Inspection of meat quality using organoleptic test and eber, as well as bacterial contamination test salmonella sp. From this study indicate that the results showed 100% (37/37) normal meat quality testing. From the six traditional market areas that were sampled, it showed that there were 5 markets that showed positive salmonella sp. and 1 negative market. Socialization about salmonellosis disease to reduce the incidence of salmonellosis as a control effort needs to be done. Prevention of bacterial contamination of salmonella sp. can be done during maintenance until the time of processing. Keywords: Chicken meat; Organoleptic; Salmonella sp.; Surabaya; Traditional market   Abstrak  Konsumsi daging merupakan salah satu usaha untuk pemenuhan protein, karena protein pada daging (protein hewani) lebih lengkap dibandingkan dengan protein pada tumbuhan (protein nabati). Daging ayam lebih banyak dikonsumsi oleh masyarakat di Indonesia dibandingkan daging sapi, karena memiliki harga yang lebih terjangkau dan tetap memiliki nilai gizi yang tinggi. Tingginya kandungan gizi pada daging menjadikannya sebagai agen tempat berkembangnya mikroba infeksius yang memungkinkan timbulnya food poisoning. Salmonella merupakan salah satu bakteri yang banyak menyebabkan keracunan makanan. Infeksi bakteri ini pada hewan atau manusia dapat mengakibatkan penyakit dengan gangguan pada bagian saluran pencernaan atau gastroenteritis seperti kram perut dan diare yang berlangsung empat sampai tujuh hari. Penelitian ini menggunakan 37 sampel daging ayam bagian dada yang diambil di beberapa pasar tradisional di Surabaya barat, dan dianalisis secara deskriptif. Pemeriksaan kualitas daging menggunakan uji organoleptis dan eber, serta uji cemaran bakteri salmonella sp. Dari penelitian ini menunjukkan bahwa Hasil penelitian menunjukkan 100 % (37/37) pengujian kualitas daging yang normal. Dari keenam wilayah pasar tradisional yang diambil sampel menunjukkan bahwa terdapat 5 pasar yang menunjukkan positif cemaran salmonella sp. dan 1 pasar negatif. Sosialisasi tentang penyakit salmonellosis untuk menekan kejadian salmonellosis sebagai upaya pengendalian perlu dilakukan. Pencegahan cemaran bakteri salmonella sp. dapat dilakukan saat pemeliharaan sampai saat pengolahan. Kata kunci: Daging ayam; Organoleptis; Pasar tradisional; Salmonella sp.; Surabaya
OUTBREAK LEPTOSPIROSIS DENGAN VEKTOR TIKUS PADA DAERAH RAWAN BANJIR DI SURABAYA Freshinta Jellia Wibisono; Sheila Marty Yanestria
JURNAL KAJIAN VETERINER Vol 4 No 2 (2016): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v4i2.1015

Abstract

Surabaya was the second largest city in Indonesia and also a city that had the flood-prone areas. Flooding that occurred caused some cases of the disease, one of the disease is leptospirosis. Leptospirosis was an acute infectious disease caused by the harmful bacteria Leptospira and rats were the main reservoir. Flooding were transmission medium of Leptospira derived from rat urine. This case study was an observational descriptive study using case control study design to describe the incidence of leptospirosis in terms of the presence of disease agents Leptospira sp in rat vector in Surabaya flood-prone areas Surabaya. Data of rats infected in flood prone areas obtained from the results of rapid test Lepto Tek Lateral Flow to identify the presence of Leptospira in serum of rats. Data of the presence of Leptospira in rats and flood-prone are were analyzed descriptively. Results showed 100% negative Leptospira Sp. on samples of blood serum with an examination of Leptotek Lateral Flow in flood prone areas in Surabaya showed that during the study started in May 2016 to October 2016 there were no mice infected with the bacterium Leptospira sp. in flood prone areas Wonokromo, Bendul Merisi, Kupang Krajan and Sidomulyo.
INSIDEN PEREDARAN AYAM TIREN PADA PASAR TRADISIONAL DI SURABAYA Sheila Marty Yanestria; Freshinta Jellia Wibisono
JURNAL KAJIAN VETERINER Vol 5 No 1 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i1.1023

Abstract

The aimed of this research was to know the incidence of carrion chicken sales in Surabaya traditional markets. The samples used in this study were chicken meats that had physical characteristics resembling carrion chicken. Chicken meat samples were taken from 20 traditional markets in Surabaya consisting of 5 traditional markets in North Surabaya, 5 traditional markets in East Surabaya, 5 traditional markets in south Surabaya, and 5 traditional markets in West Surabaya. Each traditional market had taken 10 samples of chicken meat each weighing 100 grams coming from different seller. The methode of carrion chicken test that used in this reseach was Malachyte Green Test by Warris method. The results showed that there were no positive sample of carrion chicken in North Surabaya, 9 positive samples of carrion chicken in West Surabaya, 24 positive samples of carrion chicken in South Surabaya, and 14 positive samples of carrion chicken in East Surabaya. The incidence of carrion chicken sales in Surabaya traditional markets showed that there were 47 positive carrion chickens or 23.5% of 200 samples taken.
Detection of the CTX-M Gene Associated with Extended-Spectrum β-Lactamase (ESBL) in Broiler Chickens in Surabaya Traditional Markets Putri, Mariana Febrilianti Resilinda; Khairullah, Aswin Rafif; Effendi, Mustofa Helmi; Wibisono, Freshinta Jellia; Hasib, Abdullah; Moses, Ikechukwu Benjamin; Fauziah, Ima; Kusala, Muhammad Khaliim Jati; Raissa, Ricadonna; Yanestria, Sheila Marty
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.320-334

Abstract

A common indicator used to examine the frequency and distribution of antibiotic resistance against other enteric bacteria in humans and animals is the commensal enteric bacterium, Escherichia coli. The transmission of plasmids harboring ESBL enzymes, primarily generated by E. coli, is the cause of this resistance. The purpose of this study was to identify the CTX-M gene in ESBL-producing E. coli from broiler chicken cloacal swabs in traditional Surabaya markets. The samples used were 96 cloacal swabs from broiler chickens in the traditional markets of Dukuh Kupang, Keputran, Pacar Keling, and Pucang. The antibiotic disks used in this study belonged to five different antibiotic classes; they are aztreonam (monobactam), chloramphenicol (phenicol), kanamycin (aminoglycoside), ciprofloxacin (fluoroquinolone), and tetracycline (tetracycline). Presumptive ESBL strains were then molecularly screened for the presence of CTX-M gene. Results revealed that out of the 96 chicken cloacal swab samples collected, 58 (60.42%) were positive for E. coli based on morphological culture, Gram staining, and biochemical tests. Additionally, 15 out of the 58 E. coli isolates recovered from broiler chicken cloacal swabs were multidrug-resistant (MDR) while 7 of E. coli isolates harbored CTX-M gene. Conclusively, this study has shown that broiler chickens sold in traditional Surabaya markets harbor MDR E. coli which possess CTX-M gene. Conditions in traditional markets with low levels of cleanliness and chickens placed close together can spread resistance genes with serious public health consequences. Therefore, it is imperative to observe good hygienic practices in Surabaya traditional markets in order to curtail the spread of MDR bacterial pathogens in the food chain.
Co-Authors Adhitya Yoppy Ro Candra Adhitya Yoppy Ro Candra Adhitya Yoppy Ro Chandra Agung Prasetyo Al arif, Mohammad Anam Arief Mardijanto Arief Mardijanto Aristi, Heni Aryaloka, Suhita Asih Rahayu Asih Rahayu Atina Atina Ayuti, Siti Rani Bunna Chrystin Rambu Uru Damayanti, Juwita Tabeta Desty Apritya Elsa Rosita Era Hari Mudji Fauzia, Kartika Afrida Fauziah, Ima Hasib, Abdullah Herry Agoes Hermadi Intan Permatasari Hermawan Khairullah, Aswin Rafif Kurnianto, Ady Kurniasih, Dea Anita Ariani Kusala, Muhammad Khaliim Jati Mardijanto, Arief Mirni Lamid Mohammad Exceltyanto Widodo Moses, Ikechukwu Benjamin Mustofa Helmi Effendi Putri, Mariana Febrilianti Resilinda Rahmaniar, Reina Puspita Raissa, Ricadonna Ratna Widyawati Riza Zainuddin Ahmad Ro Candra, Adhitya Yoppy Wardhani, Bantari Wisynu Kusuma Warsito, Sunaryo Hadi Wibisono, Freshinta Jellia Wibowo, Syahputra Widhowati, Dyah Widya Paramita Lokapirnasari Wiranata, Ferian Pandu