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All Journal El-Hayah : Jurnal Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis BIOVALENTIA: Biological Research Journal JUSTICIA SAINS: Jurnal Ilmu Hukum Journal Of Medicine And Health Sciences (Medisci) Journal Of Agriculture Sciences (Agrosci) Journal of Community Service (Abdisci)
Saswiyanti, Enny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Learning Bats Handling for Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Master, Jani; Susandi, Waryoko
Jurnal Agrosci Vol 3 No 4 (2026): Vol 3 No 4 March 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i4.1154

Abstract

Background. The only flying mammals, bats play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Bats are suspected to be related to COVID-19. Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. The life-trapping technique uses a mist net. Methods. Bat handling for taking an oral swab was carried out using the pinch grip method, holding both bat arms backwards with the thumb and middle finger, with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open, and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested and given a drink/water and released into nature. Oral swab samples from 10 individual bats, fruit-eating bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and an insect-eating bat, Scotophilus kuhlii (n = 1).
RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung Kurniasih, Vidyanti; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Pratiwi, Dian Neli
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1161

Abstract

Background. Bats are considered animals that carry infectious diseases, are known to be reservoirs of viruses, and are suspected of causing disease outbreaks. The World Health Organization stated that this disease outbreak is caused by Coronavirus Disease 2019 (COVID-19), which is caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) and in collaboration with the Lampung Disease Investigation Center, a preliminary study on EID detection on bats based on Predict Protocols was done to identify the presence of coronavirus on bats, including rural areas, Braja Harjosari, East Lampung. It is located next to Way Kambas National Park. Methods. Oral swab samples were taken from ten bats, 3 species: Cynopterus brachyotis and Cynopterus horsfieldi, and Scotophilus kuhlii. RNA extraction was conducted by Predict Protocols, which has four stages: lysis, binding, washing, and elution. Conclusion. Two samples showed thin-band luminescence. While in the quantity test, there are three samples of good concentration results between 1.8 and 2.0.
Co-Authors Ahyul Heni Arsan, Zulfi Azmi, Sevira Nur Christine Wulandari Dian Neli Pratiwi Dwi Ayu Febriyani Eko Agus Prihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elly Lestari Rustiati Fahrezi, Aditya Febriansyah, Muhammad Giyono Giyono I Wayan Tony Candra Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kurniawati, Yeyen Kusuma, Andriyani Wijaya Maharani, Annisa Lidya Mustikawati, Ganis Neli Pratiwi, Dian Novianasari, Tika Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putri, Chicka R.P. Raden Fadly Bayu Dwiyoga Ramadhan, Viki Rhamadaningtyas, Nabila Aulia Rustiati, Elly L. Sa'uddah, Lousanja D. Samsul Bakri Sevira Nur Azmi Sidik, Mahfud Srihanto, Eko A. Sukatmoko, Sukatmoko Sunandar Susandi, Waryoko Susanto, Alvin Wiwiet Vidya Kurniasih Virnarenata, Elsa Waryoko Susandi Yani, Ani Andri Yanti Yulianti Zulkarnain, Danisworo