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All Journal Biospecies El-Hayah : Jurnal Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis BIOVALENTIA: Biological Research Journal JUSTICIA SAINS: Jurnal Ilmu Hukum Organisms: Journal of Biosciences Journal Of Medicine And Health Sciences (Medisci) Journal Of Agriculture Sciences (Agrosci)
Saswiyanti, Enny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung Kurniasih, Vidyanti; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Pratiwi, Dian Neli
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1161

Abstract

Background. Bats are considered animals that carry infectious diseases, are known to be reservoirs of viruses, and are suspected of causing disease outbreaks. The World Health Organization stated that this disease outbreak is caused by Coronavirus Disease 2019 (COVID-19), which is caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) and in collaboration with the Lampung Disease Investigation Center, a preliminary study on EID detection on bats based on Predict Protocols was done to identify the presence of coronavirus on bats, including rural areas, Braja Harjosari, East Lampung. It is located next to Way Kambas National Park. Methods. Oral swab samples were taken from ten bats, 3 species: Cynopterus brachyotis and Cynopterus horsfieldi, and Scotophilus kuhlii. RNA extraction was conducted by Predict Protocols, which has four stages: lysis, binding, washing, and elution. Conclusion. Two samples showed thin-band luminescence. While in the quantity test, there are three samples of good concentration results between 1.8 and 2.0.
Detection of Environmental DNA from Tree Bark Associated with Rubbing Behavior of Dicerorhinus sumatrensis in Way Kambas National Park Priyambodo; Zulkarnain, Danisworo; Rustiati, Elly Lestari; Kurniawati, Yeyen; Putri, Chicka Refina Rahma; Srihanto, Eko Agus; Saswiyanti, Enny; Arsan, Zulfi; Sukatmoko; Pratiwi, Dian Neli; Permatasari, Nindy
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/zv2m2645

Abstract

The development of non-invasive methods is essential for monitoring critically endangered species such as the Dicerorhinus sumatrensis. This study aimed to evaluate the potential of tree bark associated with rubbing behavior as a source of environmental DNA (eDNA) for species detection in Way Kambas National Park. A total of 30 samples were collected from tree bark surfaces showing evidence of rubbing activity. DNA was extracted and analyzed using two approaches: simple electrophoresis and polymerase chain reaction (PCR)-based molecular methods. The results showed that the simple method failed to detect visible DNA bands, indicating low DNA concentration and degradation in environmental samples. In contrast, PCR amplification successfully detected DNA in 5 out of 30 samples, including hair-derived and epithelial residues associated with rubbing behavior.containing hair residues produced more reliable detection compared to those with only epithelial traces. These findings demonstrate that tree bark able to serve as a potential non-invasive source of eDNA, particularly when analyzed using sensitive molecular techniques. This approach provides an alternative method for detecting elusive species and contributes to the development of effective conservation strategies for the sumatran rhinoceros.
PERBEDAAN LAMANYA HIDUP Trypanosoma evansi PADA BERBAGAI MEDIA Lusita, Lulu; Kanedi, M.; Saswiyanti, Enny; Setyaningrum, Endah; Valinata, Sisca
Biospecies Vol. 19 No. 2 (2026): Juli 2026 (In Press)
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i2.49174

Abstract

The diagnosis of Surra disease has limitations that make it impossible to test Trypanosoma evansi samples directly on all samples. This is due to limitations in time and medical and paramedical personnel in field diagnosis, thus requiring research on T. evansi live media solutions. The purpose of this study was to determine the lifespan of T. evansi in various media solutions and which media solution was most effective. This study was experimental in nature, using a Completely Randomized Design (CRD) and three replicates. Data analysis was performed using the LDS test, followed by an Analysis of Variance (ANOVA) test to find significant differences in the lifespan of T. evansi in various media solutions; Phosphate Buffer Saline Glucose (PBSG), Dulbeccos Modified Eagle Medium (DMEM) 1X + GlutaMAXTM, NaCl infusion (sodium chloride 0.9%), coconut water. The results of the statistical test at room temperature showed significant differences, with the Phosphate Buffer Saline Glucose (PBSG) solution sample being the best media solution for the survival of T. evansi parasites.
Co-Authors Arsan, Zulfi Azmi, Sevira Nur Christine Wulandari Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Endah Setyaningrum, Endah Fahrezi, Aditya Febriansyah, Muhammad Giyono Giyono Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Kanedi, M. Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kurniawati, Yeyen Kusuma, Andriyani Wijaya Lusita, Lulu Maharani, Annisa Lidya Mustikawati, Ganis Neli Pratiwi, Dian Novianasari, Tika Permatasari, Nindy Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putri, Chicka R.P. Putri, Chicka Refina Rahma Ramadhan, Viki Rhamadaningtyas, Nabila Aulia Rustiati, Elly L. Sa'uddah, Lousanja D. Samsul Bakri Sidik, Mahfud Srihanto, Eko A. Sukatmoko Sukatmoko, Sukatmoko Susandi, Waryoko Susanto, Alvin Wiwiet Valinata, Sisca Virnarenata, Elsa Yani, Ani Andri Yanti Yulianti Zulkarnain, Danisworo