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Konsentrasi Merkuri (Hg) pada Air Laut di Area Budidaya Teluk Manalu Kabupaten Kepulauan Sangihe Bawelle, Eunike; Undap, Suzanne L.; Kusen, Diane J.; Pangemanan, Novie P. L.; Tumembouw, Sipriana S.; Wantasen, Adnan S.; Langi, Edwin O.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.30002

Abstract

The aims of this research were to determine the concentration of mercury, and knowing the water quality in aquaculture area of Manalu Bay Sangihe Islands Regency. The method used in determine the research station is purposive sampling method or deliberate sampling with consideration that station 1 is near the gold mine, station 2 is the area aquaculture activities and station 3 is far from the gold mean and aquaculture activities. The data used in this research were sourced from primary data (sea water sampling used a sampling technique for Hg testing and direct measurement of water quality parameter) and secondary data (journals, books and other supporting documents that support research). The result detected the concentration of Hg content at station 1, 2 and 3 were <0,0003 mg/l below the sea water quality standards for marine biota (0,001). The parameter quality data of the water are temperature ranged between 31-32 0C, pH 6,8-7,1 and salinity 32-34 ppt. In general, the presence of chemical physics parameters in Manalu Bay was good condition and suitable for aquaculture after being compared with Kep. MENLH No. 51/2014, Attachment III concerning Sea Water Quality Standards for Marine Biota.
Seleksi probiotik Lactobacillus sp. dari usus ikan mas (Cyprinus carpio) potensial untuk akuakultur Bella, Sintia S.; Manoppo, Henky; Undap, Suzanne L.; Tumbol, Reiny A.; Ngangi, Edwin L. A.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.29902

Abstract

The aimed of the study was to isolate and select the probiotic Lactobacillus from carp intestine potential for use in aquaculture. Carp as a source of probiotics with a size of about 200 g was  taken from the Center for Fish Hatchery and Disease Control (BP3I), Tateli. After aseptic surgery, the intestine was separated and cleaned, weighed as much as 1 g, put in a mortar and crushed until smooth. The scoured results were put into a tube and added 9 ml NaCl to get a ratio of 1:10. The tube that already contains intestinal scouring was then centrifuged at a speed of 1000 RPM for 10 minutes. The centrifugation process was carried out 2 times to get the supernatant that has been cleared of debris. The supernatant was removed and diluted to obtain a concentration of 10-2  to 10-3. The solution was then spread on MRS (De Man Rogosa and Sharpe Agar) media and incubated at 28oC in the Incubator for 24-48 hours. The bacteria that grew in culture fmedia was reisolated several times to get a single pure colony.. The pure colonies were propagated in the same media to be further identified through gram staining, biochemical, proteolytic, amylolytic, cellulolytic, antibiotic resistance, antagonistic, pathogenicity tests. The results of the isolation obtained the characteristics of a colony of milky white color, round and convex with long cells, gram-positive and confirmed based on biochemical tests as Lactobaccilus sp. The bacterium also had proteolytic, amylolytic, cellulolytic, antagonistic, antibiotic-sensitive activity that were characterized by the formation of clear zones in the area around probiotic discs, and did not cause death either as a disease or as a result of poisoning. Thus it can be concluded that Lactobaccilus sp. isolated from carp intestines can be used as probiotics for cultivation.
Accumulation of heavy metals (As, Cd, Pb, Hg) on brown algae, Padina australis, cultivated in Kima Bajo Waters, North Minahasa Regency Hidayat, Sarif; Mantiri, Desy M.H.; Paulus, James J.H.; Lasut, Markus T.; Rumampuk, Natalie D.C.; Undap, Suzanne; Sumilat, Deiske A.
AQUATIC SCIENCE & MANAGEMENT Vol 9, No 1 (2021): APRIL
Publisher : AQUATIC SCIENCE & MANAGEMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.9.1.2021.32470

Abstract

Although heavy metals have relatively low levels in water column and sediments, they will not be degraded and can even be absorbed and accumulated by marine algae. Research on accumulation of heavy metals on algae was done in Kima Bajo Waters, North Minahasa Regency. The research objective was to evaluate the accumulation of four heavy metals, i.e. arsenic (As), cadmium (Cd), plumbum (Pb), and mercury (Hg), on brown macroalgae Padina australis. The alga was introduced and cultivated in the research area using bottom method. The same method was also used in the experiment. One-week acclimatization process was done prior the experiment. Sampling of alga’s thallus was carried out every two weeks to measure the concentration of metals. The experiment was terminated after six weeks. Bottom sediment was also collected at the beginning of the experiment to measure the metal concentration. The results showed that the heavy metals (As, Cd, Pb, and Hg) were accumulated in the algae. The highest concentration of Cd, Pb, and Hg accumulated in the algae was 0.15 mg/kg wet weight, 3.5 mg/kg wet weight, and 0.009 mg/kg wet weight, respectively. All the concentrations were higher than the initial concentration of each metal. Accumulation of As was also occurred with the highest concentration (1,9 mg/kg wet weigh) occurred at fourth weeks; however, it was lower than the initial concentration. The accumulation varied according to type of metals and time; accumulation of Cd, Pb, and Hg were occurred on second weeks of cultivations and As on the fourth weeks. It can be concluded that cultivated alga can accumulate heavy metals.Indonesian title: Akumulasi logam berat (As, Cd, Pb, Hg) pada alga coklat, Padina australis, yang dibudidaya di Perairan Kima Bajo, Kabupaten Minahasa Utara
Efektivitas beberapa bahan alami yang ditambahkan dalam pakan sebagai pemicu pertumbuhan ikan Nila (Oreochromis niloticus) pada sistem mina padi Karisoh, Yuriani S.; Manoppo, Henky; Mudeng, Joppy D.; Ngangi, Edwin L. A.; Undap, Suzanne L.
e-Journal BUDIDAYA PERAIRAN Vol 9, No 1 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.1.2021.30316

Abstract

This study aimed to examine the effect of several types of natural substances in increasing the growth of tilapia optimally. The fish were taken from Board of Fisheries and Freshwater Aquaculture Tatelu (BPBAT), North Sulawesi Province. Fish were acclimatized for a week in ponds in the “mina padi” system, with a density of 25 fish/net. After acclimatization, the fish were fed pellet with the addition of ginger (Zingiber officinale), garlic (Allium sativum Linn), and yeast (Saccharomyces cerevisiae) for 30 days as much as 10% /fish body weight /day with the frequency of feeding twice a day, namely at 08.00 and 16.00. This study used a completely randomized design (CRD) with four treatments, namely treatment A: ginger flour 7.5 g/kg of feed, treatment B: garlic flour 15 g/kg of feed, treatment C: Yeast 5 g/kg of feed and treatment D: commercial feed (control), each had three replications. The weight of fish was scaled weekly. The results showed that the growth of fish fed with the addition of baker’s yeast was better than fish fed with the addition of ginger, garlic, and commercial feed, but statistically the growth of fish fed with baker’s yeast and ginger were not significantly different from each other.
Kelayakan lokasi budidaya ikan Kuwe (Caranx sp.) ditinjau dari parameter fisika kimia kualitas air pada karamba jaring apung di Desa Tuntung Timur Kabupaten Bolaang Mongondow Utara Datunsolang, Frayogi; Kusen, Diane; Kalesaran, Ockstan J.; Undap, Suzanne L.; Sambali, Hariyani; Wantasen, Adnan S.; Salindeho, Indra
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.34127

Abstract

This study aimed: (1) to analyze the physical and chemical parameters of water quality of the giant travelly fish (Caranx sp) culture area in floating net cages (KJA) in Tuntung Timur Village, Bolaang Mongondow Utara Regency, and (2) to analyze the feasibility of water quality of the giant travelly fish (Caranx sp) in east Tuntung Village, North Bolaang Mongondow Regency. The  physical and chemical parameters of water at the cultivation site of Caranx sp in floating net cages were measured in situ, including temperature, salinity, brightness, dissolved oxygen and pH. Chemical physics parameters in floating net cages, namely: temperature ranges from 28.6 oC - 33.3 oC, salinity 30 - 33 0/oo, pH 7.6 - 7.9, oxygen content 3, 9 - 4.3 mg / l and water transparency of 7.4 m - 9.9 m. The giant travelly fish aquaculture in KJA in east Tuntung village is an enlargement activity, the seeds were stocked from bagan and the surrounding waters, the size of the seeds ranged from 8-12 cm, and the stocking density was 500-600 fish. Maintenance was carried out for 5-6 months and harvested at sizes 300-4000 gr/individu. The water quality surrounding KJA was ideal for fish farming activities.
Studi kualitas air di area akuakultur Desa Eris Kabupaten Minahasa Sinaga, Rohana; Undap, Suzanne L.; Kusen, Diane J.; Pangemanan, Novie P. L.; Mudeng, Joppy D.; Pangemanan, Jeannette
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.34744

Abstract

The aim of this study was to monitor the condition of water quality in the aquaculture area of fix net cage culture system (KJT) at Lake Tondano, Eris Village, Eris District, Minahasa Regency which included temperature, pH, DO, TDS, Turbidity, NO2, NH3, PO4  and sediment (N, Nitrogen and P, Phosphorus) in  a different time. This research was conducted from March to May 2021 using a survey method. The research activities consisted of direct measurements in the field (in situ) using a Horiba U-5000G instrument and laboratory analysis (ex situ) at the BTKLPP Class 1 Laboratory and the BARISTAND Laboratory in Manado for primary data. Primary data was carried out at 3 points, Point 1 represented around residential activities, Point 2 where the areas of cultivation activities, and Point 3 where outside of cultivation and settlement activities. Primary data was compared with secondary data i.e, publish articles and then processed in the STORET method. Data were evaluated to the water quality parameters standard according to the Indonesian Government Regulation number 22 of 2021. The results showed that the water temperature ranged between 26.3 - 28.07 0C, Turbidity (10.5 - 24.4 NTU), TDS (0.167 - 0.172 g/L), pH (3.79 - 7.84), DO (1.87 - 9.05 mg/L), NH3 (0.12 - 0.26 mg/L) , NO2 (0.001 - 0.064 mg/L), PO4 (0.2 mg/L - 1.67 mg/L). In the sediment samples, N was found in the  ranged of 0.20% - 0.26% and  P (0.48% - 2.69%). It can be concluded, the condition of water quality in the aquaculture area using KJT in Eris Village is categorized as class C (medium polluted) and N in the sediment criteria is moderate and the content of P in the sediment criteria is very low.
Identifikasi bakteri patogen Aeromonas sp. pada ikan Nila (Oreochromis niloticus) di Desa Matungkas, Kecamatan Dimembe, Kabupaten Minahasa Utara Sinubu, Wulan V.; Tumbol, Reiny A.; Undap, Suzanne L.; Manoppo, Henky; Kreckhoff, Reni L.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36633

Abstract

The purpose of this study was to identify the bacteria Aeromonas sp. in a tilapia (Oreochromis niloticus) culture pond in Matungkas Village, Dimembe District, North Minahasa Regency. Bacterial identification was carried out at the Laboratory of the Fish Quarantine Center for Quality and Safety Control of Fishery Products, Manado. The time of the study was carried out from February 2021 - June 2021. Ten samples of fish were selected from 3 existing ponds for this study with the criteria of fish that have clinical signs of disease infection such as pale body surface, red spots and ulcers. From 10 samples, 19 isolates were obtained to be identified. The bacterial method was carried out based on the results of gram tests and biochemical tests which included: Gram Tests, Motility Test, Oxidase Test, Oxidative Fermentative Test, Tolerance Test to incubation temperature, Tolerance Test to pH of the media, Tolerance Test to the concentration of NaCl in the media and rimler – shotts selective media test. Of the 19 isolates from 10 samples identified, there were 7 isolates that were positive for A. hydrophila, namely: N1.4.1, N2.1.1, N2.1.2, N2.2.1, N2.3.2, N3.3.1, and N3.3.2. Twelve other isolates were negative for A. hydrophila. The characters of A. hydrophila identified are creamy in colour, gram negative, oxidase positive, bacteria tolerance to temperature 4 °C positive, 37 °C positive, 50 °C negative, can move (motile), fermentative, pH tolerance test media pH 3 negative, pH 5 positive, pH 9 positive, pH 11 positive, tolerance test for NaCl concentration 0.5% positive, 3% positive, 5% negative, rimler – shotts positive. All sampling sites (ponds) in Matungkas Village were infected with bacteria.
Anatomical characteristics of macroalgal species from Bombuyanoi Island, East Bolaang Mongondow Regency, North Sulawesi Patra, Frian; Kepel, Rene Ch.; Lumingas, Lawrence J.L.; Gerung, Grevo S.; Kondoy, Khristin F.; Sumilat, Deiske A.; Undap, Suzanne L.
AQUATIC SCIENCE & MANAGEMENT Vol 9, No 2 (2021): OCTOBER
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v9i2.35229

Abstract

This research aims to study the anatomical structure of macroalgae. Sample of macroalga was collected by using Line Intercept Transect (LIT) method with sampling quadrat. Macroalgae samples were dried and separated from the roots, stems, leaves and receptacles (if present). Samples were cut transversely and longitudinally at each section and observed under binoculared microscope of Olympus CX with monitor. Based on the histology, anatomical structure of macroalgae species can be divided into two tissues from outside to inside, namely cortex and medullary cells. The cortex is composed of one layer or more. The cortex is the area between the epidermis and the central column. The medulla cells only have one layer which is the largest layer. Based on the observation result of cells type from observed spesies Eucheuma denticilatum, Gracilaria arcuata, Hydropuntia edulis, G. salicornia, Hypnea valentiae, Turbinaria deccurens, and T. ornata, it shows that cells of medulla become small toward cortex.Indonesian title:  Karakteristik anatomi jenis makroalga dari Pulau Bombuyanoi, Kabupaten Bolaang Mongondow Timur, Sulawesi Utara
Kajian kualitas air untuk menunjang perikanan budidaya Danau Moaat, Provinsi Sulawesi Utara Paputungan, Firgiawan; Pangemanan, Novie P. L.; Tumbol, Reiny A.; Undap, Suzanne L.; Tumembouw, Sipriana S.; Rantung, Steelma V.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.37130

Abstract

The purpose of this study was to measure several physical and chemical parameters of Mooat Lake waters and to assess the feasibility of water quality in Mooat Lake waters to support aquaculture.Primary data were obtained from direct sample measurements in the field (in situ) and laboratory analysis. In situ sample measurement data consisted of temperature, degree of acidity (pH), dissolved oxygen (DO), and turbidity. Sampling of water at all research stations used a modified bottle that was labeled accordingly as a water sample holder. After the water sample is taken in the field, stored in a cooler, then taken to the Laboratory of Environmental Health Engineering and Disease Control (BTKLPP) class 1 Manado and the Manado BARISTAND Laboratory for examination of levels of Nitrite (NO2), Nitrate (NO3), Ammonia (NH3) , and Phosphate (PO4). Based on research data of several water quality parameters measurements and analysis show that temperature (26,17oC), Turbidity (11,20 NTU), pH (5,6), DO (8,31), nitrite (0,04005 mg/l), nitrate (0,705 mg/l), ammonia (0,145 mg/l), and phosphate (1,005 mg/l). These results show that the waters of Mooat Lake is still feasible and meets the water quality standard values to support aquaculture activities. Furthermore, it is strengthened by the analysis of the suitability of the location for the development of aquaculture which shows that the water quality conditions of Mooat Lake are classified as suitable.
Kelayakan kualitas air untuk budidaya ikan Kuwe (Caranx sp.) pada kurungan jaring apung di Perairan Kelurahan Makawidey Mamesah, Yulia; Undap, Suzanne L.; Tumembouw, Sipriana S.; Kusen, Diane J.; Pangemanan, Novie PL.; Monijung, Revol D.
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.51628

Abstract

Abstract The aim of study was to determine the feasibility of the quality of Kuwe fish (Caranx sp.) aquaculture waters in floating net cages (KJA) in Makawidey Village, Bitung City. The research method used was purposive with primary data (water quality data: brightness, temperature, depth, salinity, pH, DO) and secondary data (various existing documents and literature that can support research). Prior to data collection, a preliminary study was carried out to determine the research point. The data obtained during the research were analyzed and displayed in the form of tables and graphs and presented descriptively. The results of measuring water quality parameters are; Brightness (Point 1: 2,5 m ; Point 2: 3,1 m ; Point 3: 3,3 m), Depth ( Point 1 : 2.7 m ; Point 2: 9 m ; Point 3: 26,80 m); Temperature (Point 1: 28.40 ± 1.35; Point 2: 27.48 ± 0.90C; Point 3: 27.31 ± 1.36) ; Salinity (Point 1: 31.59±14.5 ; Point 2: 31.57±14.6; Point 3: 31.55±14.6 ppt); pH (Point 1: 8.90±4.9; Point 2: 7.43±3.44; Point 3: 7.20±3.29) and Dissolved Oxygen (Point 1: 9.60±4.39; Point 2: 9.13 ± 4.25; Point 3: 9.13 ± 4.20 mg/l), has a value that is in accordance with seawater quality standards stipulated in Government Regulation Number 22 of 2021 concerning Implementation of Protection and Management Environment. Makawidei waters meet the requirements to be used as a location for aquaculture in terms of water quality parameters and produce a suitable category for aquaculture biota. Keywords: waters environment, psycho-chemical parameters, site selection, sea farmingAbstractThe aim of study was to determine the feasibility of the quality of Kuwe fish (Caranx sp.) aquaculture waters in floating net cages (KJA) in Makawidey Village, Bitung City. The research method used was purposive with primary data (water quality data: brightness, temperature, depth, salinity, pH, DO) and secondary data (various existing documents and literature that can support research). Prior to data collection, a preliminary study was carried out to determine the research point. The data obtained during the research were analyzed and displayed in the form of tables and graphs and presented descriptively. The results of measuring water quality parameters are; Brightness (Point 1: 2,5 m ; Point 2: 3,1 m ; Point 3: 3,3 m), Depth ( Point 1 : 2.7 m ; Point 2: 9 m ; Point 3: 26,80 m); Temperature (Point 1: 28.40 ± 1.35; Point 2: 27.48 ± 0.90C; Point 3: 27.31 ± 1.36) ; Salinity (Point 1: 31.59±14.5 ; Point 2: 31.57±14.6; Point 3: 31.55±14.6 ppt); pH (Point 1: 8.90±4.9; Point 2: 7.43±3.44; Point 3: 7.20±3.29) and Dissolved Oxygen (Point 1: 9.60±4.39; Point 2: 9.13 ± 4.25; Point 3: 9.13 ± 4.20 mg/l), has a value that is in accordance with seawater quality standards stipulated in Government Regulation Number 22 of 2021 concerning Implementation of Protection and Management Environment. Makawidei waters meet the requirements to be used as a location for aquaculture in terms of water quality parameters and produce a suitable category for aquaculture biota.Keywords: waters environment, psycho-chemical parameters, site selection, sea farming
Co-Authors Adnan Wantasen Alex D. Kambey Ali Djamhuri Ari B. Rondonuwu Bawelle, Eunike Bella, Sintia S. Datunsolang, Frayogi Deiske Adeliene Sumilat, Deiske Adeliene Diany R.A Silalahi Dolvinus Wakman Duyoh, Marini Edwin D Ngangi Farnis B. Boneka Fransine B. Manginsela Grevo S Gerung Henky Manoppo Henneke Pangkey Indra R.N. Salindeho Indra Salindeho Indri Manembu Joppy Mudeng Juliaan Ch. Watung Kabalmay, Agmilda A. Karisoh, Yuriani S. Kepel, Rene Ch. Khristin I. F. Kondoy, Khristin I. F. Kreckhoff, Reni L. Kusen, Diane J. Langi, Edwin O. Lawrence J. L. Lumingas Lawrence J.L. Lumingas, Lawrence J.L. Leleran, Andreas J. P. L. Lumingas, Aaron R. T. Mamangkey, Noldy G.F Mamesah, Yulia Mamonto, Reva E. Mantiri, Desy M. H Markus T. Lasut Medy Ompi Melani, Hellen Mokolensang, Jeffrie F. Monijung, Revol D. Monijung, Revol J. Natalie D Rumampuk Ngangi, Edwin. L. A. Ockstan J. Kalesaran Pangemanan, Jeannette Pangemanan, Novie P. L. Pangemanan, Novie P.L. Pangemanan, Novie PL. Pangemanan, Pankie L. Pangemanan1, Novie P. L. Pankie N.L Pangemanan Paputungan, Firgiawan Patra, Frian Paulus, James Pilipus A Urbasa Pratasik, Gerald A. Putra, Stevano F.P Reiny A. Tumbol Robert J Rompas Sabono, Mateda M. Salaki, Meiske S. Sambali, Hariyani Sammy N. J. Longdong Sammy N.J. Longdong Sangari, Joudy R.R Sarif Hidayat, Sarif Silvester B Pratasik Simanjorang, Daniel Sinaga, Rohana Sinubu, Wulan V. Sipriana S. Tumembouw Steelma V. Rantung Tamba, Ermaulina Telaumbanua, Beatus H. Telaumbanua, Yedieli Tokah, Christian Zebblon, Passion Ch.