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Sarasati Windria
Departemen Ilmu Kedokteran Dasar, Divisi Mikrobiologi, Fakultas Kedokteran, Universitas Padjadjaran. Program Studi Kedokteran Hewan, Fakultas Kedokteran, Universitas Padjadjaran
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Demam tifoid sistemik yang disebabkan oleh Salmonella gallinarum pada ayam broiler: Bukti patologis dan mikrobiologis Azzahra, Tiara; Maharani, Devina Aisyah; Tsevaot, Amar Adonay; Hartady, Tyagita; Prayugo, Armanda Dwi; Windria, Sarasati; Krissanti, Ita; Satrio, Faisal Amri
ARSHI Veterinary Letters Vol. 9 No. 4 (2025): ARSHI Veterinary Letters - November 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.4.99-100

Abstract

Salmonella gallinarum, the causative agent of fowl typhoid, is a highly pathogenic bacterial disease associated with substantial mortality in poultry, predominantly affecting adult chickens and turkeys. This study aimed to describe the pathological lesions and microbiological characteristics of S. gallinarum infection in chickens with fowl typhoid. S. gallinarum is a gram-negative, rod-shaped, facultative anaerobic bacterium with an optimal growth temperature of 37 °C and the ability to survive across a broad pH range (4.0–9.0). Necropsy findings in affected chickens revealed interstitial pneumonia of the lungs, intestinal hyperemia with petechial hemorrhages, hepatomegaly, splenomegaly, and fatty degeneration of the cardiac tissue. Bacterial culture of intestinal swab samples on MacConkey agar demonstrated the growth of pale-yellow colonies consistent with S. gallinarum. Microscopic examination confirmed Gram-negative bacilli, while biochemical testing showed an alkaline/acid reaction, absence of gas and H₂S production, non-motility, and negative indole and urease reactions
IIsolasi dan Identifikasi Staphylococcus aureus pada Susu Sapi Mastitis Subklinis di Pangalengan, Jawa Barat Sylvany, Hemalya; Waskita, Pranyata Tangguh; Windria, Sarasati
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.87051

Abstract

Susu sapi merupakan produk utama yang dihasilkan sapi perah. Kandungan susu sapi yang beragam, menjadikan susu sebagai sumber nutrisi yang baik untuk metabolisme tubuh. Tingginya permintaan susu di Indonesia belum terpenuhi karena adanya aspek kesehatan dari sapi yang mengalami mastitis. Staphylococcus aureus adalah salah satu patogen yang menyebabkan mastitis subklinis pada sapi. Tujuan dari penelitian ini adalah untuk mengetahui cemaran Staphylococcus aureus pada sampel susu sapi yang menderita mastitis subklinis di Pangalengan. Penelitian ini dilakukan dengan metode deskriptip kualitatif, menggunakan 48 sampel susu sapi perah mastitis dengan hasil uji CMT . Isolasi dan identifikasi Staphylococcus aureus dilakukan dilakukan melalui metode kultur pada media Blood Agar Plate (BAP), Mannitol Salt Agar (MSA), pewarnaan Gram, uji katalase, uji DNase, serta uji koagulase. Dari 48 sampel terdapat 17 isolat yang mengandung Staphylococcus aureus. 17 isolat menghasilkan hemolisin pada blood agar, memfermentasikan mannitol, pewarnaan Gram positif kokus, katalase positif, koagulase positif dan DNase positif.  Kata kunci : mastitis subklinis; susu sapi; Staphylococcus aureus.  
OCCURRENCE OF MULTIPLE INFECTIONS IN CATTLE AT A FARM IN THE CIBIRU AREA, WEST JAVA Putri, Risti Yupiesta; Permadi, Anindya Putri; Sevaot, Amar Adonay; Cyntia, Annisa Permana; Windria, Sarasati; Krissanti, Ita; Satrio, Faisal Amri; Septiyani; Prayugo, Armanda Dwi
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p07

Abstract

The health of beef cattle is a crucial aspect of livestock management. Effective health management in cattle serves to minimize potential economic losses caused by diseases that commonly affect beef cattle. These diseases may be caused by bacteria, fungi, viruses, as well as blood and gastrointestinal parasites. Multiple infections may occur, particularly when suboptimal animal conditions and environmental factors support disease transmission. This case report aims to describe the occurrence of multiple infections and to identify the causative infectious agents in a beef cattle farm located in Cibiru, West Java. The case involved a 4-year-old Limousin cow that exhibited clinical signs including nasal discharge, hypersalivation, diarrhea, and tick infestation. Samples collected for diagnostic examination included nasal discharge swabs, blood, and fecal samples. Nasal swab samples were cultured on blood agar plates (BAP) and MacConkey agar (MCA), followed by primary tests including Gram staining, catalase test, coagulase test, and biochemical tests. Blood samples were examined using blood smear evaluation and differential leukocyte counts. Fecal samples were examined using native smear, flotation, sedimentation, and McMaster techniques. The results revealed a case of multiple infections, with the identification of Staphylococcus aureus and Klebsiella pneumoniae, as well as a gastrointestinal parasitic infection (coccidiosis). In addition, infestation with Rhipicephalus ticks contributed to blood parasitic infections, including babesiosis and anaplasmosis. These concurrent infections were associated with a compromised immune status and reduced production performance, potentially leading to economic losses for the farmer. Therefore, preventive and control measures, including proper sanitation and biosecurity, are essential, as infected animals and contaminated environments may act as sources of disease transmission.
Necropsy Findings, Isolation and Identification of Salmonella pullorum in Layer Chickens in Sumedang, Indonesia:  A Case Report Maharani, Briliant; Cyntia, Annisa Permata; Azra, Putri Zivana; Hartady, Tyagita; Windria, Sarasati; Prayugo, Armanda Dwi
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Pullorum disease or Chalky feces, caused by Salmonella pullorum bacteria, is a highly contagious bacterial infection that significantly affects commercial poultry, particularly in developing countries. This study was aimed to report a case of pullorum disease in commercial layer chickens in Sumedang, West Java, Indonesia. The diagnostic approach included clinical examination based on clinical signs, followed by pathological and microbiological identification. Clinical signs observed were lethargy, white diarrhea (chalky feces) and decreased egg production. Necropsy findings revealed pathological changes including hepatomegaly, splenomegaly, caseous masses in the caecum and joints, oophoritis and white nodules in the heart. Additional anatomy pathological findings included tracheal hyperemia and malacia in the brain. Bacterial isolation and identification were conducted from internal organs using MacConkey Agar, followed by Gram staining and biochemical tests including Kligler’s Iron Agar (KIA), Motility Indole Urea (MIU) and Simmons’s Citrate Agar. The results confirmed the presence of S. pullorum bacteria, characterized by non-motile, Gram-negative bacilli. These findings demonstrate that S. pullorum can cause systemic infection affecting multiple organs. Based on pathological and microbiological examinations, it was concluded that the disease case in the layer chicken samples was caused by chronic infection of S. pullorum. This report highlights the importance of early diagnosis and improved biosecurity measures to prevent outbreaks, reduce mortality rates and minimize economic losses in Chicken farming.
Co-Authors ., Septiyani Adi Imam Cahyadi Adi Imam Cahyadi Adi Imam Cahyadi Afiat Berbudi Alifya Azzahra Cahyaningtyas ANGGAENI, TRIANING TYAS KUSUMA Arimdayu, Annisa Retno Arimdayu, Annissa Retno Astri Gloria Larwuy Azra, Putri Zivana Azzahra, Tiara Baasin, Rusdi Toriq Bangkit, Azmi Firman Chairunnisa, Arvia Chen, Vincentius Owen Cyntia, Annisa Permana Cyntia, Annisa Permata Darmesti, Daniswara Danindra Dini Oktaviani Dini Wahyuni Eka Rifki, Prananda Faisal Amri Satrio Ghassani, Yumni Khairina Hanafiah, Septiani Purwanti Hermawan, Firda Aisyah Hesti Lina Wiraswati Hesti Lina Wiraswati Hilma Halimatus Sadiah Hilmi, Haitsam Muhammad Julia Ramadhanti Khairani, Shafia Khaq, Arina Al Krissanti, Ita Larasati, Savira Azhari Lestari, Nurul Aeni Ayu Lia Faridah Maharani, Briliant Maharani, Devina Aisyah Meri Alex Sandra Muhammad Saifuddin B. S. Arif Mutiara, Chika Khaerani Nadila, Nasywa Nadya, Siti Faridha Amalia Ihsanu Najwa Namira Nurfitri, Fauzia Nurjanah, Gina Siti Okta Wismandanu Permadi, Anindya Putri Prasaja, Aditya Gilang Prayugo, Armanda Dwi Prianti, Nadiyah Dwi Putri, Alysa Gema Fatiah Putri, Risti Yupiesta Rachmawati, Nadya Rahma, Hana Nayla Rifki, Prananda Eka Rosdianto, Aziiz Mardanarian Salma Nur Raidah Santoso, Fransiskus Teguh Satryo, Faisal Amri Savira Ekawardhani Septiyani Setyaputra, Muhammad Farkhan Sevaot, Amar Adonay Siti Faridha Amalia Ihsanu Nadya Syafrizal Saragih Syamila, Nafira Hylma Sylvany, Hemalya Triasmara, Wahyu Tsevaot, Amar Adonay Tyagita Hartady Waskita, Pranyata Tangguh Wendi Prameswari, Wendi Wijaya, Stevanie Andrea Wuri, Rahmitiana Yasfira Aradella