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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Sainstek Journal of Marine Research JURNAL ILMU PERTANIAN Bioedukasi: Jurnal Pendidikan Biologi Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Peuradeun Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrosains: Jurnal Penelitian Agronomi Jurnal Biologi Tropis Formatif: Jurnal Ilmiah Pendidikan MIPA Assimilation: Indonesian Journal of Biology Education Jambura Edu Biosfer Journal Jambura Journal of Health Sciences and Research Jambura Journal of Chemistry BIOSFER : Jurnal Biologi dan Pendidikan Biologi Jurnal Review Pendidikan dan Pengajaran (JRPP) RADIAL: JuRnal PerADaban SaIns RekAyasan dan TeknoLogi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Spizaetus: Jurnal Biologi dan Pendidikan Biologi Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Jurnal Kewarganegaraan Nusantara: Jurnal Pendidikan Indonesia Journal of Research in Instructional BIOMA : Jurnal Biologi dan Pembelajarannya BIOVALENTIA: Biological Research Journal Makara Journal of Science JURNAL BIOLOGI PAPUA Journal of Tropical Mycorrhiza Jurnal Rekayasa elektrika Jurnal Biologi Al-Hayat: Natural Sciences, Health & Environment Journal Biodik: Jurnal Ilmiah Pendidikan Biologi HUMANIORASAINS Tumbuhan : Publikasi Ilmu Sosiologi Pertanian dan Ilmu Kehutanan Science: Indonesian Journal of Science J-CEKI RADIAL: Jurnal Peradaban Sains, Rekayasa dan Teknologi
Novri Youla Kandowangko
Universitas Negeri Gorontalo
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Journal : Jurnal Biologi Tropis

 1 
Effect of BAP and NAA on Callus Emergence Time of Dumbaya Young Leaf Explants in Vitro Walangadi, Farhana Rahmatia; Ahmad, Jusna; Pagalla, Devi Bunga; Kandowangko, Novri Youla; Febriyanti, Febriyanti
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9045

Abstract

Momordica cochinchinensis, locally known as Dumbaya in Gorontalo Province, is a traditional medicinal plant with underutilized potential due to the extremely hard morphology of its seed coat. This physical barrier limits the penetration of water, air, and nutrients, thereby reducing the success rate of seed germination and plant propagation. The challenge of propagating plants that are difficult to reproduce sexually, such as dumbaya, can be addressed through various approaches, one of which is asexual reproduction using tissue culture techniques. This study aims to address propagation constraints by applying tissue culture techniques supplemented with Plant Growth Regulators (PGRs), specifically BAP (Benzyl Amino Purine) and NAA (Naphthalene Acetic Acid), which are critical factors in successful in vitro plant regeneration. The combination of Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) is a commonly used Plant Growth Regulator (PGR) for inducing callus formation and organogenesis, where BAP is effective in stimulating shoot formation, while NAA plays a role in root induction. The research employed an experimental method using a Completely Randomized Design (CRD) with five treatment levels: H0: MS + 0 ppm NAA + 0 ppm BAP; H1: MS + 1.5 ppm NAA + 0.5 ppm BAP; H2: MS + 3.0 ppm NAA + 0.5 ppm BAP; H3: MS + 1.5 ppm NAA + 1.0 ppm BAP; and H4: MS + 3.0 ppm NAA + 1.0 ppm BAP. Each treatment was replicated three times. The results showed that treatment H1 produced the earliest callus formation, with an average callus initiation time of 7 days after planting.
Comparison of Anatomical Structure of Maize (Zea mays L.) Plant of Gorontalo Local Variety Pulut and Momala Ahaya, Cindi; Kandowangko, Novri Youla; Febriyanti, Febriyanti; Ahmad, Jusna; Pagalla, Devi Bunga
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8483

Abstract

One of the primary agricultural commodities that contributes significantly to global food security is maize (Zea mays L.), a monoecious cereal plant of the Poaceae family with distinct but identical male and female blooms. Local cultivars like Pulut and Momala in Gorontalo have exceptional promise that requires further study, particularly with regard to their anatomical makeup. This study Comparing the two types' stem and leaf anatomical features, including vascular tissue (xylem and phloem), epidermis, and stomata, is the goal of this study. A qualitative descriptive approach was used to make the observations, which were subsequently evaluated and shown as a comparison table. The results showed that at the age of 30 and 40 HST, the network of vessels (xylem and phloem) and epidermis on the stems and leaves of Pulut and Momala corn varieties reflected a development pattern that supported the transportation of water, minerals, and nutrients. The number of stomata in Pulut varieties decreased due to less than optimal growth, while Momala varieties showed an increase in the number of stomata which was influenced by the growth period with environmental conditions suitable for corn plants.  
Co-Authors Abubakar Sidik Katili Abubakar Sidik Katili Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Ahmad, Aisyah Akbar, Muh Nur Alfandi Kibu Ali, Fathur Rahman M. Andi Pratiwi Andi Pratiwi Ani Hasan, Ani Aryati Abdul Aryati Abdul Bakari, Iswanto Bambang mamangkay Bulowe, Yuniarty R Chairunnisa Lamangantjo Cono, Titi Hawanda Metania Dali, Ira K. Damopolii, Insar Devi Bunga Pagalla Devi Bunga Pagalla Dewi Wahyuni K. Baderan Djafar, Fitri Yani Djuna Lamondo Dunggio, Fadlia Elawati, Elawati Elya Nusantari Fathur Rahman M. Ali Fatmariani Abdul Wahab Febriyanti Febriyanti , Febriyanti febriyanti febriyanti Febriyanti Febriyanti Febriyanti Febriyanti Frida Maryati Yusuf GIAT SURYATMANA Gintulangi, Sabriana Oktaviana Gogou, Elsi Hamzah B Uno Husain, Holiyana Husain, Ilyas H. Ilyas Husain Indri Meliyani Bouta Indriati Husain Isima , Lindawaty Jusna Ahmad Kadita P. Latif Keley, Umar Keley, Umar Labuga, Falerins ladoma, wafiq aziza m. Lamatenggo, Nina Lindawaty Isima Margaretha Solang Marini Susanti Hamidun Masra Latjompoh Miftahul Fikriyah Adudu Muchtar Ahmad Muh. Nur Akbar Mukhlisulfatih Latief Mustamin Ibrahim Mustaqimah, Nur NENNY NURLAENY Nunaki, Jan Hendriek Nunaki, Jan Hendriek Nunaki, Jan Hendriek Nurdin Nuryanti Rumalolas Pagala, Devi Bunga Pagalla , Devi Bunga Pakaya, Melisa Patta Sija Putri, Tarissa Eka Rahman, Iyam Rahwilin Van Gobel Ramli Utina Rampi Yusuf Rianjani, Devi Tutut Rianjani, Devi Tutut Rinna Amelia Polihito Rizal Suleman Rizaldi Mokodompit ROBERT DJONGGI MARULI SIMANUNGKALIT Rosely, Meilin S. Y. Sahat Patar Lumban Gaol Saifullah Mamonto Suwarni Karim Syafar, Sitti Zaenab Tadidik, Ulfaida H. Ui, Maryam Walangadi, Farhana Rahmatia Wilinda A. Datau Wirnangsi Din Uno Wirnangsih D. Uno Yelsi Patiung Yuliana Nurdian Toini Yuliana Retnowati Yumna Binti Abud Alamri Yusrin Anton Puluhulawa Zuliyanto Zakaria Zusriadi, Brenda Febrina