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All Journal Jurnal Penelitian Sains Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Current Biomedicine Mukhtabar: Journal of Medical Laboratory Technology Jurnal Kesehatan Siliwangi Jurnal Pengabdian Masyarakat Kesehatan Indonesia Jurnal Info Kesehatan
Merdekawati, Fusvita
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Optimization of Concentration and Staining Duration of Methyl Green in The Examination of Escherichia coli DNA Bands Using Agarose Gel Electrophoresis Adrian Prasetya, Rifky; Merdekawati, Fusvita; Iin Nur Indra, Asep; Djuminar, Ai
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.696

Abstract

Background & Objective: Nowadays, many alternative dyes are used for staining DNA during electrophoresis, one of which is Methyl Green. Methyl Green has several advantages, including being cheaper than EtBr, having low toxicity, being non-carcinogenic, and as a cationic dye, Methyl Green is resistant to photobleaching. This study aims to determine the optimal concentration and staining duration of Methyl Green as a DNA dye for examining Escherichia coli DNA bands using agarose gel electrophoresis. Method: The research method used is experimental. Amplified Escherichia coli 16S rRNA gene DNA, sized 584 bp, which has undergone electrophoresis, was stained with Methyl Green dye at concentrations of 0.10%, 0.15%, 0.20%, 0.25%, 0.00015%, 0.00020%, 0.00025%, and 0.00030% with varying immersion times of 10, 15, 20, and 25 minutes. Result: The resulting DNA bands were analyzed or measured for surface area using ImageJ software. The mean value for each experimental group was calculated. The highest mean value was used as the basis for determining the most optimal condition. Conclusion: This study concludes that the optimal concentration and staining duration of Methyl Green, based on the highest mean value of 19,844,845, is 0.00030% Methyl Green with a staining duration of 25 minutes.
STABILITAS REAKSI ENZIMATIK KADAR TRIGLISERIDA SERUM METODE Glyserol Phosphate Oxidase Para-Aminoantipyrin (GPO-PAP) DENGAN VARIASI WAKTU INKUBASI Endrique Putri, Salmanda; Kurnaeni, Nani; Nurhayati, Dewi; Merdekawati, Fusvita
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the triglycerides test lipid profile examination that is often analyzed because it provides information about cardiovascular health and stroke. Laboratory services have an important meaning in making a diagnosis based on the patient's health history and are an integral part in achieving quality laboratory results that have accurate accuracy and precision. Analytical factors that can influence examination results include incubation time. The delay in the length of incubation time is due to the limited number of laboratory personnel and inadequate equipment. The objective of this study was to assess the impact of stability on serum triglyceride levels using the GPO-PAP method with different incubation durations. The research employed a quasi-experimental design, utilizing serum samples from three Medical Laboratory Technology students. The samples were subjected to varying incubation times of 10, 60, 75, 90, and 105 minutes at room temperature, with each condition being repeated five times. The mean results of serum triglyceride levels with incubation times of 10, 60, 75, 90, and 105 minutes respectively were 80,0 mg/dL, 79,00 mg/dL, 76.36 mg/dL, 73.65 mg/ dL, and 67.94 mg/dL. Data analysis was conducted using the General Linear Model (GLM) test, with significance indicated by the Sig value. < 0.05 means that triglyceride levels with an incubation time of 75 minutes are statistically unstable.
OPTIMASI VARIASI VOLTASE DAN WAKTU TERHADAP KUALITAS PITA DNA ESCHERICHIA COLI PADA PROSES ELEKTROFORESIS GEL AGAROSA nur amani putri, afifah; Iin Nur Indra, Asep; Merdekawati, Fusvita; Khoirul Abror, Yogi
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Background: Escherichia coli is one of the causes of foodborne illness. Conventional PCR is a PCR method that is carried out qualitatively followed by visualization on agar electrophoresis. Agarose gel electrophoresis is a technique that is often used in various fields of science to separate a mixture of DNA on agarose substrate. This method is used to perform qualitative analysis of DNA samples. In electrophoresis, there are factors that affect the movement of DNA molecules, one of which is voltage. In addition, the length of time of the electrophoresis process can also affect the effectiveness of the results and the rate of migration. When the electrophoresis process time is short, large DNA fragments still tend to stick. Purpose: Therefore, it is necessary to balance the voltage and time given to get good DNA banding results. Methods: The research unit that will be used is the result of amplification of Escherichia coli 16SrRNA gene DNA measuring 584 bp. The electrophoresis process was carried out with voltage variations of 50, 100, 150 volts and time variations of 30, 45, 60 minutes. Observation of electrophoresis results that have formed DNA bands will be measured the area of DNA bands using ImageJ application. Conclusion: Based on the results of the study concluded that: The optimum voltage in the electrophoresis process in obtaining good E.coli DNA bands is at a voltage of 150 volts. The optimum time in the electrophoresis process in obtaining good E.coli DNA bands is for 30 minutes.
Optimasi Konsentrasi dan Waktu Kontak Ekstrak Daun Jati (Tectona grandis) sebagai Alternatif Safranin pada Pewarnaan Gram Syafitri, Andita Izmi; Sufa, Hafizah Ilmi; Kurniati, Iis; Merdekawati, Fusvita
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Safranin is a cationic dye and is one of the harmful substance, in addition to its relatively expensive price, safranin waste also has several harmful effects on health, so the various studies on the use of natural dyes as an alternative of safranin have been widely carried out. Teak leaves are one of the plants that have the potential to used as an alternative of safranin because they contain anthocyanin compounds. This study aims to determine the concentration and optimal contact time of teak leaf extract that can be used as an alternative of safranin. The research method used is a quasi-experiment by providing treatment with 25%, 50%, and 100% concentration variations also contact time variations of 0.5, 1, and 1.5 minutes. The results showed that teak leaf (Tectona grandis) extract could be used as an alternative of safranin in Gram staining of E. coli and S. aureus. The conclusion of this study is that the optimum concentration of teak leaf extract is 50%, and the optimum contact time of teak leaf extract in Gram staining is 0.5 minutes.
Assessment of Analytical Specificity in qPCR: SYBR Green and TaqMan Probe Methods for Isonazid-Resistant Mycobacterium tuberculosis Merdekawati, Fusvita; Suryawan, Muhammad Raihan; Nurhayati, Betty; Rinaldi, Sonny Faisal; Juliastuti, Aditya
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.793

Abstract

Background & Objective: Mycobacterium tuberculosis, often referred to as M. tuberculosis, is an infectious pathogen that is responsible for causing tuberculosis (TB), a prevalent condition that is a leading cause of death globally. The spread of tuberculosis bacteria that are resistant to certain medicines, such as isoniazid, is currently on the rise. Therefore, molecular testing, such as quantitative polymerase chain reaction (qPCR), is needed to rapidly and reliably identify isoniazid-resistant tuberculosis germs. Method: The purpose of this study was to assess the analytical specificity of TaqMan Probe and SYBR Green qPCR techniques for the detection of isoniazid-resistant tuberculosis. This study was descriptive and quantitative. Analytical specificity was ascertained using MTB DNA with the S315G mutation spiked with E. coli DNA. A paired t-test was used to assess the primary data. Result: The results show that the analytical specificity values based on the significance of the paired t-test for the SYBR Green and TaqMan Probe methods were 0.398 and 0.790, respectively. Conclusion: Based on these results, it can be concluded that the analytical specificity of the TaqMan Probe qPCR method was 1.99 times greater than that of the SYBR Green method.
Hubungan Kadar Kolesterol Total Metode CHOD-PAP dan Kadar C-Reactive Protein (CRP) Metode HS-CRP pada Penderita Obesitas Zanuba, Haifanisya Zahra; Nurhayati, Dewi; Merdekawati, Fusvita; Kurnaeni, Nani
Jurnal Penelitian Sains Vol 26, No 3 (2024)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v26i3.1049

Abstract

Obesitas adalah penumpukan lemak yang abnormal atau berlebihan di jaringan adiposa, yang dapat menyebabkan berbagai masalah kesehatan termasuk hiperkolestrolemia. Peningkatan kadar asam lemak bebas di jaringan adiposa yang tebal dapat memicu produksi C-Reactive Protein (CRP) di dalam hati. Peningkatan CRP sering kali menjadi indikator adanya peradangan sistemik terkait obesitas. Penelitian ini bertujuan untuk mengetahui hubungan antara kadar kolesterol total metode CHOD-PAP dan kadar CRP metode HS-CRP pada penderita obesitas. Jenis penelitian yang digunakan adalah deskriptif korelatif dengan desain cross-sectional. Populasi penelitian ini adalah mahasiswa Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Bandung dengan IMT > 25. Sampel sebanyak 30 orang diambil dengan teknik quota sampling. Data dianalisis dengan uji Korelasi Pearson. Hasil penelitian ini menunjukkan adanya hubungan antara kadar kolesterol total yang tinggi dengan peningkatan kadar CRP pada penderita obesitas. Dari 30 responden, 15 orang (50%) memiliki kadar kolesterol total normal dan 15 orang (50%) meningkat, sementara untuk kadar CRP, 14 orang (47%) normal dan 16 orang (53%) meningkat. Nilai koefisien korelasi antara Kolesterol Total dan CRP adalah 0.419, yang menunjukkan adanya hubungan sedang antara kadar kolesterol total dan kadar CRP pada penderita obesitas.
Persistence of Antibody Response Against SARS-CoV-2 After Vaccination Bekti, Heri Setiyo; Habibah, Nur; Dharmawati, I Gusti Agung Ayu; Merdekawati, Fusvita; Noviar, Ganjar
JURNAL INFO KESEHATAN Vol 21 No 4 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss4.1066

Abstract

SARS-CoV-2 is the causative agent of the disease known as COVID-19. COVID-19 is spreading very fast around the world. One of the immune responses that play a role in against SARS-CoV-2 infection is the production of antibodies, which is 3 weeks after infection. Where within 3 weeks after infection, antibodies will be produced against RBD and the S1 and S2 domains in glycoprotein S and nucleocapsid protein N. The ability of an antibody to inhibit viral infection is determined by its level or titer. This study aims to determine the description of antibody levels against SARS-CoV-2 after vaccination. This type of research is descriptive research. Measurement of antibody levels for SRBD SARS-CoV-2 was carried out using the CLIA method using the MAGLUMI tool. Of the 30 respondents, 23 people had received the third vaccine. The results of this study showed that the average level of SRBD antibodies against SARS-CoV-2 in respondents with 2 doses of vaccine (1.063,786 BAU/mL) was higher than in respondents with 3 doses of vaccine (535.651 BAU/mL). Vaccine intervals of more than 6 months (908.338 BAU/mL) have higher antibody levels than respondents with vaccine intervals of 1-6 months (228.006 BAU/mL). The conclusion of this study is the highest antibody titers are produced >6 months after vaccination, antibody titers are still detectable after 12 months of vaccination, and for further research, it can be measured antibody levels against SARS-CoV-2 from people who have got vaccination for a duration of 2 years or more.
DIAGNOSTIC TEST OF MOLECULAR RAPID TEST AGAINST REAL-TIME PCR ON DETECTING BCR-ABL CHRONIC MYELOID LEUKEMIA Pratama, Resta Ratna Juwita; Nurhayati, Betty; Merdekawati, Fusvita; Hayati, Eem; Ernawati, Ernawati
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 17 No 2 (2025): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v17i2.2865

Abstract

Chronic Myeloid Leukemia (CML) is a condition characterized by abnormal proliferation of hematopoietic cells and can occur across various age groups. Detection of Breakpoint Cluster Region-Abelson (BCR-ABL) is essential to identify the presence of the Philadelphia chromosome (Ph 1 chr). Real-Time Polymerase Chain Reaction (Real-Time PCR) is the gold standard for BCR-ABL detection, but it has limitations such as long processing time and high cost. The Molecular Rapid Test (TCM) using the GenXpert platform offers a faster and more practical alternative. This study aimed to evaluate the performance of TCM BCR-ABL compared to Real-Time PCR. A cross-sectional design was applied with 54 samples obtained using consecutive sampling based on Lemeshow’s formula. The results showed a sensitivity of 91%, specificity of 90%, positive predictive value (PPV) of 94%, and negative predictive value (NPV) of 86%. Wilcoxon test analysis revealed no significant difference between the two methods (p=0.100). In conclusion, TCM demonstrates diagnostic performance comparable to Real-Time PCR and has the potential to be used as a rapid and efficient alternative for CML detection.
UPAYA PREVENTIF PENYALAHGUNAAN NARKOBA MELALUI EDUKASI, SOSIALISASI DAN PEMERIKSAAN NARKOBA PADA SISWA/I SMK TI PEMBANGUNAN KAMPUS III Merdekawati, Fusvita; Abror, Yogi Khoirul; Solihat, M. Firman; Khairunnisa, Syirin Nisrina; Rahmawati, Tia; Dzulhizza, Zaskia Puteri
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat Kesehatan Indonesia In Progress
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v4i2.4221

Abstract

Drug abuse among teenagers has become a chronic issue in Indonesia, which is currently in a state of drug emergency. The misuse of narcotics, dangerous drugs, and other addictive substances can cause various negative impacts on users' lives, leading to mental and physical damage, as well as the destruction of social relationships, morals, careers, and finances. The problem of drug abuse among youth is not easy to solve, as it requires collaboration from multiple parties, including the government, police, community members, the media, families, schools, and the teenagers themselves. The main goal of this community service program was to provide students at SMK TI Pembangunan Campus III with a comprehensive understanding of narcotics and their effects on life. The program used a structured method that involved: Pre-test: To assess the students' initial understanding of drugs. Education and Socialization: To provide knowledge and awareness. Urine Screening: As a screening tool for drug use among students. Post-test: To evaluate the increase in understanding after the program. The program successfully increased students' understanding of drugs. This finding strongly suggests that the educational program was effective in achieving its goal. The improvement in understanding was reflected not only in the rise of average scores but also in the consistent improvement among almost every individual student who was measured. These positive results imply that the methods and curriculum used in the program are worth maintaining or even developing further.
Co-Authors Abror, Yogi Khoirul Adrian Prasetya, Rifky Dewi Nurhayati Djuminar, Ai Dzulhizza, Zaskia Puteri Endarwati, Dwi Veni Endrique Putri, Salmanda Ernawati Ernawati Febriyanti, Intan Hardiana, Acep Tantan Hayati, Eem Heri Setiyo Bekti I Gusti Agung Ayu Dharmawati Iin Nur Indra, Asep Iis Kurniati Indra , Asep Iin Nur Indra, Asep Iin Nur Irawan, Danni Juliastuti, Aditya Khairunnisa, Syirin Nisrina Khoirul Abror, Yogi Kurnaeni, Nani Lesiani, Bunga Rossa Nina Marliana, Nina Noviar, Ganjar nur amani putri, afifah Nur Habibah Nurhayati, Betty Nurhayati, Nuli Pratama, Resta Ratna Juwita Rahmawati, Tia Rinaldi, Sonny Faisal Rinaldi, Sonny Feisal rohayati rohayati Saputra, Fitrianingsih Shabrina, Almas Simangunsong, Kristina Solihat, M. Firman Sufa, Hafizah Ilmi Suryawan, Muhammad Raihan Syafitri, Andita Izmi Tantan, Acep Trimulyani, Annisa Zanuba, Haifanisya Zahra Zuri Rismiarti