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Merdekawati, Fusvita
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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OPTIMIZATION AND EFFICIENCY ANALYSIS OF REAL-TIME PCR FOR LEPTOSPIRA SPP. DIAGNOSIS BASED ON THE LIPL32 GENE Sulaeman, Rizal Pratama; Rohayati; Merdekawati, Fusvita; Sundara Mulia, Yuliansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10646

Abstract

Leptospirosis is a widespread zoonotic infection that endangers the health of both humans and animals, particularly in tropical and subtropical regions. Therefore, timely, sensitive and specific laboratory confirmation is essential for early clinical management. The LipL32 gene is a highly conserved virulence factor in pathogenic Leptospira. Real-time PCR provides rapid detection with high sensitivity and specificity. This study optimized real-time PCR conditions by evaluating annealing temperatures (60°C, 61°C, 62°C), primer concentrations (500 nM, 700 nM, 900 nM), and probe concentrations (250 nM, 300 nM). PCR efficiency was analyzed using absolute quantification with serial DNA dilutions (100 to 10⁻⁴). The optimal conditions were 60°C annealing temperature, 500 nM primers, and 300 nM probes. Near-ideal efficiency (97%) was achieved at 60°C with 500 nM primers and 250 nM or 300 nM probes, while 103% efficiency was observed at 61°C with 500 nM primers and 250 nM probes. This optimization enhances Leptospira detection accuracy using real-time PCR.
Korelasi Kadar C-Reactive Protein (CRP) dengan Kadar Hemoglobin A1c (Hba1c) pada Penderita Diabetes Melitus Tipe 2 Zahrani, Azka Hasbia; Khoirul Abror, Yogi; Marliana, Nina; Merdekawati, Fusvita
Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Vol 6 No 2 (2025): Desember 2025
Publisher : Fakultas Sains, Teknologi, dan Analsisi Institut ilmu Kesehatan Bhakti Wiyata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56399/jst.v6i2.314

Abstract

Diabetes melitus tipe 2 adalah kondisi meningkatnya kadar gulosa yang disebabkan oleh kesalahan fungsi insulin oleh sel beta pankreas. HbA1c digunakan sebagai penanda kontrol glikemik jangka panjang sementara CRP merupakan biomarker inflamasi yang digunakan sebagai biomarker inflamasi sistemik pada penderita diabetes melitus tipe 2. Tujuan penelitian ini untuk mengetahui korelasi CRP dengan HbA1c pada penderita diabetes melitus tipe 2. Desain penelitian yang digunakan adalah analisis observasional dengan desain cross-sectional terhadap 30 pasien diabetes melitus tipe 2 di RSUD Al-Ihsan Baleendah. Kadar CRP diukur menggunakan metode Fluorescence Immunoassay (FIA) dan kadar HbA1c diperoleh melalui rekam medis pasien. Hasil penelitian menunjukkan rata-rata CRP sebesar 11,26 mg/L dan HbA1c sebesar 9,8%. Hasil uji korelasi Spearman menunjukan nilai signifikasi p = 0,002 dengan nilai koefisien korelasi r = 0,552 yang artinya terdapat korelasi positif yang signifikan antara kadar CRP dan HbA1c. Temuan ini mengindikasikan bahwa peningkatan kadar CRP, sebagai indikator peradangan sistemik, berhubungan dengan buruknya kontrol glikemik pada pasien diabetes melitus tipe 2.
Sensitivitas dan Spesifisitas Analitik Primer Gen HNF1A Menggunakan Metode qPCR Pada Pasien Terduga MODY3 Wandalira, Yuane; Merdekawati, Fusvita; Rinaldi, Sonny Feisal; Hadiana, Acep Tantan
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 18 No 1 (2026): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v18i1.2860

Abstract

Background: Maturity onset diabetes of the young (MODY) is a rare monogenic type of diabetes, but it accounts for 1–5% of diabetes cases and typically presents before the age of 25. Among the 14 subtypes, MODY type 3 (MODY3) is the most common, particularly in Asian populations, and is associated with mutations in the hepatocyte nuclear factor 1 alpha (HNF1A) gene. Diagnosing MODY often presents challenges due to its varied clinical symptoms. Molecular testing based on qPCR offers a more accurate, relatively time-efficient, and cost-effective method for detecting HNF1A gene mutations. Objective: This study aimed to validate the sensitivity and specificity of the primer for exon 4 of HNF1A in patients suspected of having MODY3 using qPCR with SYBR Green. Methods: The samples included patients under 25 years old with a family history of diabetes mellitus, without obesity or ketoacidosis. Sensitivity was analyzed using probit regression based on amplification results from 10 DNA template dilutions with six replications. Analytical specificity was tested using a paired t-test against Plasmodium vivax spike DNA with three replications. Results: The analytical sensitivity of the HNF1A exon 4 primer was 3.16 × 10⁻⁷ ng/µL. The specificity test showed a p-value < 0.05, indicating no significant difference between target DNA and spike DNA variations. Conclusion: The HNF1A exon 4 primer demonstrated high sensitivity and good specificity, supporting its potential for qPCR-based detection of MODY as a preliminary validation.
Co-Authors Abror, Yogi Khoirul Adrian Prasetya, Rifky Dewi Nurhayati Djuminar, Ai Dzulhizza, Zaskia Puteri Endarwati, Dwi Veni Endrique Putri, Salmanda Ernawati Ernawati Febriyanti, Intan Hadiana, Acep Tantan Hardiana, Acep Tantan Hayati, Eem Heri Setiyo Bekti I Gusti Agung Ayu Dharmawati Iin Nur Indra, Asep Iis Kurniati Indra , Asep Iin Nur Indra, Asep Iin Nur Irawan, Danni Juliastuti, Aditya Khairunnisa, Syirin Nisrina Khoirul Abror, Yogi Kurnaeni, Nani Lesiani, Bunga Rossa Nina Marliana, Nina Noviar, Ganjar nur amani putri, afifah Nur Habibah Nurhayati, Betty Nurhayati, Nuli Pratama, Resta Ratna Juwita Rahmawati, Tia Rinaldi, Sonny Faisal Rinaldi, Sonny Feisal Rohayati rohayati rohayati Saputra, Fitrianingsih Shabrina, Almas Simangunsong, Kristina Solihat, M. Firman Sufa, Hafizah Ilmi Sulaeman, Rizal Pratama Sundara Mulia, Yuliansyah Suryawan, Muhammad Raihan Syafitri, Andita Izmi Tantan, Acep Trimulyani, Annisa Wandalira, Yuane Zahrani, Azka Hasbia Zanuba, Haifanisya Zahra Zuri Rismiarti