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All Journal Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Manuju : Malahayati Nursing Journal 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Ilmiah Tatengkorang Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat Mulawarman Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat
Mutiarawati, Diah Titik
Poltekkes Kemenkes Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Journal : Health Notions

 1 
Liquid Chitosan from Corbula faba Hinds on Soil Transmitted Helminth (STH) in Pakcoy (Brassica chinensis L.) Diah Titik Mutiarawati
Health Notions Vol 6, No 4 (2022): April
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60407

Abstract

Worms are a health problem that is still commonly found in developing countries. One of the causes of helminthiasis is Soil-Transmitted Helminth (STH). The worms that cause worm problems are Ascaris lumbricoides, Trichuris trichiura, Hookworm (Necator americanus and Ancylostoma duodenale). Chitosan has been discovered to have a quality positively charged polycationic, leading to changes in cell permeability. Chitosan can be found in the framework of Crustacea sp., such as white mussel shell. The purpose of this research was to determine the effectiveness of liquid chitosan from white mussel shells (Corbula faba Hinds) against Soil-Transmitted Helminth on Pakcoy (Brassica chinensis L.). The research results at a concentration of 2% on average worm larvae death time of 5.25 minutes, 1.5% concentration on average worm death time of 5.15 minutes, the concentration of 1% and 0.5% did not occur death of worm larvae. There is a significant difference in the exposure of liquid chitosan from white shell to death time of worm larvae. These results show that the liquid chitosan from white mussel shell to Soil-Transmitted Helminth on mustard meat is effective at a concentration of 1.5% with an average death time of 5.15 minutes. Keywords: Soil-Transmitted Helminth; chitosan; Corbula faba hinds
Phytochemical Screening and In Vitro Antibacterial Activity of Green Tea (Camellia Sinensis L) Extract Against Staphylococcus Epidermidis Hardian Bimanto; Yuyun Dwi Wahyuni; Diah Titik Mutiarawati; Lully Hanni Endarini
Health Notions Vol 4, No 8 (2020): August
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn40805

Abstract

This study aimed to determine phytochemical screening and ethanol extract activity of the green leaves against Staphylococcus epidermidis. The microbial activity of the ethanol extract was carried out using the diffusion and dilution method with concentrations used of 1.5%, 2.5%, 3.5%, 4.5%, and 5.5% accompanied by three repetitions. The results showed that the ethanol extract of green tea leaves contained alkaloids, saponins, steroids or triterpenoids, flavonoids, polyphenols, and tannins and had the largest inhibition zone diameter of 30mm at a concentration of 4.5%. KHM (Minimum Inhibitory Content) at a level of 4.5% and KBM (Minimum Kill Rate) at a concentration of 5.5%. Analysis of the data with the normality test then proceed with the Kruskal Wallis test obtained a value of P = 0.008. With the value P
Rice Bran as Opportunity Media for Candida albicans Increase Diah Titik Mutiarawati
Health Notions Vol 5, No 12 (2021): December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn51205

Abstract

The incidence of invasive fungal infections caused by fungi of the genus Candida is reported to be increasing in various countries. C. albicans can be isolated and cultured in liquid media, one of which is PDB (Potato Dextrose Broth), a common medium used for the growth of yeasts and moulds. Bekatul or rice bran is a waste from rice milling, which still contains nutrients such as carbohydrates and protein, which are needed as energy sources for mushroom growth. Colonies were C. albicans grown in bran liquid media at mass concentrations of 20 grams, 40 grams, 60 grams, 80 grams and 100 grams with PDB as the gold standard. The colonies C. albicans growing were then confirmed by planting on PDA media (Potato Dextrose Agar), and microscopic testing was performed. The results showed that C. albicans grew well in bran liquid medium at pH 5.5-5.0, which was incubated for 1-5 days at a temperature of 25°C-30°C and was characterized by the growth of white colonies on the bottom of the tube, while the number of C. albicans that grew on liquid bran media was more than that of PDB media. The conclusion of this research is that the liquid bran media can be used as an alternative medium for PDB for the growth of the fungus C. albicans.Keywords: white rice bran; Candida albicans; alternative media; potato dextrose broth
In Vitro Anthelmintic Activity of Acalypha Indica Leaves Extracts Diah Titik Mutiarawati
Health Notions Vol 4, No 3 (2020): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.354 KB) | DOI: 10.33846/hn40305

Abstract

Anting-anting leaves (Acalypha indica L) is one of the wild plants (weeds) that are often used by the community to treat helminthic diseases such as ascariasis, containing several potentially anthelmintic compounds, namely saponins, tannins and flavonoids. The purpose of this study was to determine the effect of giving Anting-anting leaf extract (Acalypha indica L) as an anthelmintic to the time of death of pork roundworms (Ascaris suum Goeze). The method in this study was pre-experimental with a post test only group design. The subjects of the study were Ascaris suum. The study was conducted at the Parasitology Laboratory of the Surabaya Polytechnic Health Medical Laboratory Technologist Department in December-May 2018. This study used 5 treatment groups, 0.9% NaCl as negative control and 0.25% pirantel pamoate as positive control and 60%, 80% and 100%. Data were analyzed using the Kolmogrov-Smirnov test, then the Kruskal-Wallis test was then continued using a Post-Hoc test to determine the difference in anthelmintic power of ear leaf extract of each concentration with positive control over the time of worm death. The average time of Ascaris suum worm death at a concentration of a concentration of 60% for 228.5 minutes, a concentration of 80% for 174 minutes, and a concentration of 100% for 92 minutes. So it can be concluded that the leaf extract of Acalypha L has an anthelmintic effect on the Ascaris suum worm. Keywords: anthelmintic; Ascaris suum; anting-anting leaf
Statistical Test of Two Way Anova Block Design as a Decision Making Tool for Analyzing the Growth Results of Staphylococcus aureus Bacteria in Sorghum Media (Sorghum bicolor L. Moench) Sri Sulami Endah Astuti; Diah Titik Mutiarawati
Health Notions Vol 6, No 12 (2022): December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn61202

Abstract

Statistics has wide uses for making the right decisions in various areas of life, because statistics give a person the knowledge and ability to evaluate data. Through this research, researchers hope to create a new learning model from the results of the development of the Two Way Anova Design Block Statistical Test that can be used as a decision-making tool in the analysis of data on the growth results of Staphylococcus aureus bacteria on alternative media from sorghum media. The results obtained in this study, that in the variation of the sorghum period 4 grams, 5 grams, and 6 grams both in dilution with aqua distillation and meat broth the growth of Staphylococcus aureus bacteria in contrast to the growth in mannitol salt agar (positive control) media. Meanwhile, in the periodic variation of 7 grams of sorghum in both aqua distillation and meat broth, there is no difference in the growth in mannitol salt agar media. Two way Anova block design, used if the experimental material is grouped. So that each group aranges a replication for the treatment. The treatment was given randomly to each group. Based on statistical method approach block design, we find alternative mannitol salt agar media into sorghum.  
Co-Authors ., Pestariati Alda Nugrahini Anita Dwi Anggraini Anita Dwi Anggraini Astuti Setiyani Bambang Hadi Sugito Darjati Dedi Kurniawan Della Ika Putri Damayanti Demes Nurmayanti Endah Astuti, Sri Sulami Endarini, Lully Hani Endarini, Lully Hanni Era Fitria Yunita Evi Yunita N Evy Diah Woelansari Hadi Suryono Hardian Bimanto Harianto Notopuro Heni Nuril Arfiyanti Ilma Ilma Indah Lestari, Indah Ira Puspita Sari Isfentiani, Dina Istanto, Wisnu Juliana Christyaningsih Junaedi, Winda Rachmawati Khambali, Khambali Kiaonarni OW Lembunai Tat Alberta, Lembunai Tat Luthfi Rusyadi Mamik Mamik Minarti Minarti Muhammad Sholakhuddin Al-Ayubi Museyaroh, Museyaroh N. S. Widodo Nastasya Nunki Nur Hatijah Pengge, Nuning Marina Prayekti, Endah Puspita, Ria Puspitadewi, Teresia Retna Rahayuningsih, Christ Kartika Retno Sasongkowati Retno Sasongkowati Salsyabillah Ramadhani HD. Widya Sasongkowati, Retno Siagian, Hotmaida Siti Alfiah Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Vemy Rahmany Kurniawan Win Darmanto Wisnu Isntanto Yusianti Silviani Yuyun Dwi Wahyuni