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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Kedokteran Brawijaya Jurnal Kedokteran INDONESIAN JOURNAL OF PHARMACY Promotif: Jurnal Kesehatan Masyarakat AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat JP.JOK (Jurnal Pendidikan Jasmani, Olahraga dan Kesehatan) Jurnal Ilmu Kedokteran dan Kesehatan Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Journal of Health Sciences Medical and Health Science Journal KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Leverage, Engagement, Empowerment of Community (LeECOM) ASEAN Journal of Community Engagement Community Development Journal: Jurnal Pengabdian Masyarakat AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Budapest International Research and Critics in Linguistics and Education Journal (Birle Journal) Medical Scope Journal (MSJ) COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat SENTRI: Jurnal Riset Ilmiah Prominentia Medical Journal Jurnal Ilmiah Kesehatan JPM Pambudi Holistik Jurnal Kesehatan Jurnal Pendidikan Jasmani, Olahraga dan Kesehatan (Jp.jok)
Florence Pribadi
Universitas Ciputra
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Journal : Medical Scope Journal (MSJ)

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Efek Pemberian Suplementasi Kombinasi Vitamin C dan Albumin Terhadap Viabilitas Spermatozoa dalam Proses Sentrifugasi (Studi In Vitro) Maria K. A. Klau; Hudi Winarso; Florence Pribadi
Medical Scope Journal Vol. 6 No. 2 (2024): Medical Scope Journal
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/msj.v6i2.52488

Abstract

Abstract: Sperm centrifugation is an important process to obtain good spermatozoa for assisted reproductive techniques. However, the centrifugation process has additional impacts such as increased reactive oxygen species (ROS) and oxidative stress affecting sperm viability due to damage of spermatozoa membrane. The use of vitamin C (ascorbic acid) can reduce elevated ROS levels, thereby protecting DNA and spermatozoa from damage caused by oxidative stress, meanwhile albumin can reduce oxidative stress and maintain the integrity of spermatozoa membrane. This study aimed to obtain the effect of combination of vitamin C and albumin on spermatozoa viability in centrifugation process. This was a true experimental in vitro study using post-test-only control design. There were four groups in this study, as follows: the control group (K); the treatment group (P1), centrifugation without vitamin C and albumin; (P2), centrifugation with vitamin C; and (P3), centrifugation with a combination of vitamin C and albumin. The Mann-Whitney test for each group showed significantly differences in spermatozoa viability (p<0.05) among them. The treatment group receiving supplementation with a combination vitamin C and albumin produced the highest viability after the control group. In conclusion, supplementation of combination of vitamin C and albumin is capable in maintaining spermatozoa in centrifugation process. Keywords: assisted reproduction technique; centrifugation; spermatozoa viability; supplementation    Abstrak: Sentrifugasi sperma merupakan proses penting untuk mendapatkan spermatozoa yang baik dalam keperluan teknik bantu reproduksi, namun proses sentrifugasi memberi dampak lain yaitu terjadinya peningkatan reactive oxygen spesies (ROS) dan stres oksidatif yang memengaruhi viabilitas spermatozoa akibat rusaknya membran spermatozoa. Pemberian vitamin C (asam askorbat) dapat menurunkan kadar ROS yang meningkat sehingga melindungi DNA dan spermatozoa dari kerusakan akibat stres oksidatif sedangkan albumin mampu mengurangi stres oksidatif dan mempertahankan integritas membran spermatozoa. Penelitian ini bertujuan untuk mengevaluasi pengaruh kombinasi vitamin C dan albumin terhadap viabilitas spermatozoa. Jenis penelitian ialah true experimental in vitro menggunakan post test only control design dengan pengamatan pada kelompok kontrol (K); kelompok perlakuan (P1) sentrifugasi tanpa vitamin C dan albumin; (P2) sentrifugasi dengan vitamin C; dan (P3) sentrifugasi dengan kombinasi vitamin C dan albumin. Hasil uji Mann-Whitney dari masing-masing kelompok menunjukkan viabilitas spermatozoa yang berbeda bermakna (p<0,05) antar satu sama lain. Kelompok perlakuan yang mendapat suplementasi kombinasi vitamin C dan albumin menghasilkan viabilitas tertinggi setelah kelompok kontrol. Simpulan penelitian ini ialah suplementasi kombinasi vitamin C dan albumin mampu mempertahankan viabilitas spermatozoa dalam proses sentrifugasi. Kata kunci: teknik bantu reproduksi; sentrifugasi; viabilitas spermatozoa; suplementasi
Co-Authors Achmad Basori, Achmad Adam, Kamila Adinda Zharifah Putri Setiawan Agnes Atmadjaja Almer Fathoni Syahda Amadea Arum Natasha Angelarita Djami Raga Areta Idarto Arini Dyah Saputri Arisanti, Shinta Atalya Riawani Iring Azyvati Karensa Putera Azyvati Karesna Putera Bachtiar Rizki Nardiansyah Bella Meylani Lifindra Bella Meylani Lifindra Bella Meylani Lifindra Berlian Ester Wakas Berlian Ester Wakas Budi, Stephanie Laurensia Bueya, Eirene Putri Febriani Pratama Casey Clarissa Gondo Casey Clarissa Gondo Cassey Clarissa Gondo Citra Videla, Ardelia Darmanto, Arief Gunawan Denys Putra Alim Desy Natalia F. Maskim Dion, Aldy Dyaniko Prio Basudewo Ellysia Yuvena Maheswari Eric Dino Tandoyo Erik Jaya Gunawan Eva M. Anigomang Gede T.W. Suara Glenn Gilang Manyering Glory Ta’bi Palloan Gondo, Casey Clarissa Harijanto, Cyrena Theophania Hebert Adrianto Helen Octavia Djoenaydy Hudi Winarso Imam Santosa Imam Santosa, Rahajoe Imanuel D. K. Aji Intan Murni Arifah Irwin Prijatna Kusumah Irwin Priyatna Kusumah Karina Dewi, Karina Karmila A. Wardani Kencanawati, Nathania Dwi Kevin Luke Kevin Luke Kharisma A. Perdana Kusumah, Irwin Priyatna Laning, Joseph Christian Lidya Anin Lidya Handayani Lifindra, Bella Meylani Lola Adriani Lorent P. Ridfan Luke, Kevin Lyndia Effendy Manurung, Riska Enjelica Mardhika, Della Maria K. A. Klau Mega Nilam Sari Mitha A. P. Handojo Mohamad, Fawaid Mufti Yuana, Hindri Muhammad Fadhil Ihsan Pratama Muliasari Kartikawati Natalia Yuwono Nathania Dwi Kencanawati Nendra Ateja, Kerta Ni Luh Shalia Eka Pratiwi Njoto, Juli Karijati Noer, Tjandrakirana M Sjaifullah Novia Yolanda Nur Aini Nur, Farah Shania Olivia Tantana Permata Ayu Sofyan Posuma, Anastasia Ellena Pramita, Desy Hinda Raden Roro Shinta Arisanti Rafa Daniel Walukow Rahajoe Imam Santoso Raniar Nasyira Reynaldi Hardianto Saputra Ridzal Wahid Ronald Torang Marsahala Panggabean Salmon Charles Pardomuan Tua Siahaan Sampe, Jonah Alberto Santosa, Rahajoe Imam Santoso, Rahajoe Imam Sari, Nurul Illahi Kusuma Sayogo, Wiliam Setiawan, Adinda Setiawan, Adinda Zharifah Putri Siahaan, Salmon Charles Siahaan, Salmon Charles Pardoman Tua Siusanto Hadi Sofyan, Permata Ayu Stefani Nurhadi Suameitria, Desak Nyoman Surya Sucahyo, Yoselyn Sudargo, Iie Suhartati Suhartati Syahida, Nawira Tambuang, Christ Tandean, Victor Ummu Habibah, Ummu Ummul Fadika Victor Tandean Wahid, Ridzal William Tandio Putra Wiraputri, Arini Wirya, Stephen Akihiro Yasmine P. Fadhilah Yoewono, Jessica Renata Yohan Christian Suisan Zefanja Andera Gondo