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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmu Lingkungan Jurnal Lahan Suboptimal Sains Tanah IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) Molekul: Jurnal Ilmiah Kimia Journal of Tropical Marine Science Berkala Penelitian Hayati SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal

Hary Widjajanti

Jurusan Biologi FMIPA, Universitas Sriwijaya, Sumatera Selatan, Indonesia

Author-ID : 201755

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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EFFECT OF LIME APPLICATION ON INDIGENOUS NITROGEN-FIXING BACTERIA IN TIDAL SOILS MANAGED FOR THAN 30 YEARS Ridwan, Rahmawati; Budianta, Dedik; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatment

POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098 Nurkayah, Nurkayah; Nurnawati, Elisa; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 5 No. 1 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.

EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALEâ€™S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES Asiandu, Angga Puja; Widjajanti, Hary; Nurnawati, Elisa
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Endophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragonâ€™s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragonâ€™s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragonâ€™s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragonâ€™s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 Â± 4.05 mm, DTP4 against S.aureus 8.80 Â± 0.03 mm and DSP4 against S.dysentriae 10.15 Â± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 Âµg/mL, DTP4 against S.aureus was 125 Âµg/mL and DSP4 against S.dysentriae was 31.25 Âµg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus.

ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT Putri, Amanda Rahmaniah; Salni, Salni; Widjajanti, Harry
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Twelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 Âµg/ml and 20.89 Âµg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 Âµg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76Âµg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound.

POTENCY OF ENDOPHYTIC FUNGI ISOLATED FROM Muntingia calabura AS ANTIFUNGAL SUBSTANCES AGAINST Candida parapsilosis Agung Bimantara; Hary Widjajanti; Elisa Nurnawati
BIOVALENTIA: Biological Research Journal Vol. 8 No. 2 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.8.2.2022.226

Alternative is needed to overcome the problem of dandruff caused by C. parapsilosis. Endophytic fungi isolated from plants are capable of synthesizing compounds to produce various secondary metabolites. This study aimed to obtain the most active endophytic fungal isolates, determine the class of compounds, and determine the antifungal activity of the endophytic fungi (M. calabura) against C. parapsilosis. Fifteen isolates of endophytic fungi isolated from M. calabura were coded FDK1-FDK15. There was a color change in the media after 30 days of cultivation isolates of fungi. FDK4 and FDK13 have potential as antifungals. FDK13 fungal extract had the highest inhibition zone diameter of 20.00 mm. 20 eluates were obtained from the isolation of com-pounds (Thin Layer Chromatography), FDK 131, 1314, 1317, and 1320 eluates are compounds of the terpenoid group and FDK1316 eluates are phenol group compounds that are active in inhibiting yeast growth. FDK1317 was the most active compound isolate in inhibiting the growth of C. parapsilosis with an inhibition zone diameter of 12.5 mm.

ISOLATION AND PURITY DNA FROM LEAF STORAGE OF Lansium domesticum CORRESPOND FOR BARCODING ANALYSIS Nur Arifah; Laila Hanum; Sarno Sarno; Nita Aminasih; Singgih Tri Wardana; Hary Widjajanti
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.251

Lansium domesticum is one of tropical plant. Genetic studies of tropical plant species occurred very slowly due to some obstacles. Provided fresh sample for isolation step was one of impediment. Fresh sample was important conditions to avoid impure DNA template. A simple and rapid technique was needed to get a pure DNA from storage sample. The aims of this study is to see the purity of the DNA isolation results from Lansium domesti-cum mature leaf from South Sumatra that have been stored for 2 years and to test whether the DNA isolation results can be used for barcoding analysis by amplification using PCR method with rbcL primer. There are 11 sample of Lansium domesticum were taken from 8 districts in South Sumatra. The sample was the mature leaves of Lanisum domesticum. The leaves were stored in a freezer at -20oC for two years. There was one sam-ple stored within 2 weeks as a comparison. The results of the study showed the isolation of DNA from storage and mature leaves of Lansium domesticum used Plant Genomic DNA Kit DP305 had a good quantity for 7 sample. 3 sample had low purity and 1 sample contaminated by RNA. Despite electrophoresis showed the isola-tion result not intact and compact, nevertheless the PCR results showed 11 samples could be amplified using rbcL primer. This result indicated the DNA isolation method suit for barcoding analysis using PCR method.

CELLULASE ACTIVITY OF RIZOSFER BACTERIA OF KETAPANG PLANTS (Terminalia catappa L.) Rosmania, Rosmania; Widjajanti, Hary; Nurnawati, Elisa
BIOVALENTIA: Biological Research Journal Vol. 9 No. 2 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.2.2023.403

Cellulose, a natural biopolymer produced by plants, undergoes enzymatic breakdown by cellulase, cleaving the monomer's β-1,4 glycosidic bonds. Cellulolytic bacteria, producers of cellulase, can be isolated from plant rhizospheres, like Ketapang (Terminalia catappa L.), abundantly found in the Biology Department at Universitas Sriwijaya, South Sumatra, Indonesia. This study aimed to isolate cellulolytic bacteria from Ketapang rhizosphere soil and assess their cellulase activity. The spread plate method on selective Carboxy Methyl Cellulose Agar (CMC agar) was used for bacterial isolation, and cellulase activity was measured using the DNS method (3.5-dinitro salicylic acid). Results yielded 22 isolates of cellulolytic bacteria capable of growth on selective CMC agar. Thirteen isolates, identified through a screening test, produced cellulase, forming clear zones post-addition of 0.1% Congo Red and 1 M NaCl. Cellulolytic activity indices for these isolates ranged from 0.02 to 2.6 mm. The highest indices (IAS) and cellulase activities were observed in BS10, BS4, and BS22, with IAS values of 2.64, 2.12, and 1.71, and cellulase activities of 354.99 U/ml, 9.42 U/ml, and 9 U/ml, respectively. Identification results suggested that isolate BS10 bore similarities to the genus Zoogloea, while isolates BS4 and BS22 showed similarities to the genus Bacillus.

Co-Authors A. Wulandari A. Wulandari Abdul Madjid Agung Bimantara Agustina Alfarado, Daniel Alfia Rahma Kurniawati Ali Djamhuri Andika Puspita Dewi Angga Prasetya Mulya Aria Yanka Paula Putri Arum Setiawan Arum Setiawan Asiandu, Angga Puja Bambang Yudono Budiono Budiono Dade Jubaedah Dedi Rohendi Dedik Budianta E. Patriono E. Patriono Eka Amelia Elfita Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Ervina Mukharomah, Ervina Fitrya Fitrya Genti, Karta Sari Gusti Ayu Widayanti Handayani, Harminuke Eka Harminuke Eka Handayani Hary Wahyudi Heni Yohandini Laila Hanum Maraulina, Hotly Mardiyanto Mardiyanto Mardiyanto Maria Lusia Marini Wijayanti Maulida Harwati Meiyerani, Jeni Melki Melki Muharni Muharni Munawar Munawar Munawar Munawar Muslim Muslim Nabila Saraswati Neni Marlina Ni Luh Putu SR Nina Tanzerina Nita Aminasih Noviyanto NUNI GOFAR Nur Arifah Nurkayah, Nurkayah Oktiansyah, Rian Permana, Muhammad Aditya Putri, Amanda Rahmaniah Putri, Aria Yanka Paula Putri, Hanindita Alifia Rahmawati Ridwan Riris Aryawati Rosmania, Rosmania Rozirwan . Salni Salni Salni Saputra, Ade Bayu Sarno . Sarno Sarno Singgih Tri Wardana Siti Alharzsa Siti Yuliani Sri Pertiwi E Sutandar, Vivi Hendra Syarifah Tanbiyaskur Ummi Hiras Habisukan, Ummi Hiras Wuriesyliane Wuriesyliane Yustina Hapida, Yustina

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