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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Dedikasi Jurnal Gamma Journal of Agroecology Jurnal Penelitian Tanaman Industri Jurnal Sain Peternakan Indonesia Kultivasi Agriprima : Journal of Applied Agricultural Sciences FALASIFA : Jurnal Studi Keislaman Islamic Management: Jurnal Manajemen Pendidikan Islam Jurnal Ilmiah Inovasi Auladuna : Jurnal Prodi Pendidikan Guru Madrasah Ibtidaiyah Jurnal Penelitian Tanaman Industri (Littri) Tafkir: Interdisciplinary Journal of Islamic Education Al-Ashr : Jurnal Pendidikan dan Pembelajaran Dasar FAJAR Jurnal Pendidikan Islam JASNA : Journal For Aswaja Studies Jurnal Dedikasi Hukum Jurnal Pengabdian Pada Masyarakat Jurnal Edukasi Pengabdian Masyarakat: EDUABDIMAS EDUCARE: Journal of Primary Education Journal of Tropical Crop Science and Technology
Agus Zainudin
Universitas Muhammadiyah Malang
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Efficiency of Genetic Transformation via Pollen-Tube Pathway of Jatropha (Jatropha curcas L.) Based on Histochemical and Molecular Analysis Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, and
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.424 KB) | DOI: 10.24831/jai.v45i3.12925

Abstract

The genetic transformation via pollen-tube pathway is an alternative method to overcome the constraints imposed by genotype specificity in transformation and regeneration in jatropha (Jatropha curcas L.) tissue culture. Therefore, it is necessary to establish important parameters for efficient genetic transformation of jatropha via pollen-tube pathway. The objective of the research was to study the efficiency of direct transformation of jatropha via pollen-tube pathway based on histochemical and molecular analysis. Solution of purified pCAMBIA1301 DNA plasmid carrying a hptII marker gene and a gus reporter gene with concentration level of 0.05, 0.25, 0.50 µg µl-1 were applied to stigma of flowers at 1, 2, 4, 7, 10 h after pollination. Seedling of IP3A, IP3P and JcUMM18 jatropha’s genotypes derived from 15 combination treatments of plasmid DNA concentration and application time, also wild type was subjected to histochemical and molecular analyses. Based on those analyses, the efficiency of transformation via pollen-tube pathway of three jatropha genotypes ranged from 1.5-16.7%. PCR analysis showed that a number of positive plants were identified by using specific primers hptII and gus, i.e. 1-3 and 3-7 plants of the 15 combined treatments, respectively. It indicated that the transformation efficiency via the pollen-tube pathway varied in each jatropha genotype.Keywords: Jatropha curcas L., pCAMBIA1301, plasmid DNA, stigma-drip
Co-Authors Abd. Muhith Abdullah Adini, Almas Adlina Ali Ikhwan Amiq, Bahrul Aniek Iriany Aris Winaya Astutik, Anik Widya Aulia Zakia BAMBANG HELIYANTO Bambang Sapta Purwoko Charismaraya, Kefassada Dian Puji Rahayu Dini Kurniawati Dyah Nawangsari Elga Yanuardianto Erfan Dani Septia Fauzan, Fauzan Febriyanti, Beby Dwi Hadi Sudarmo HADI SUDARMO Haq, Farhan Chairul Helvi Ardana Reswari Hendry Sulistiyanto Hepni Hepni Huda, Ahmad Hisbulloh Husain, Syarief Ibrahim Ida Ayu Putu Sri Widnyani Iis Siti Aisyah Irsyad, Muammar Habibi Kholishoh, Siti Adyanur Maftuchah Maftuchah Mashudi Mashudi Masruroh, A`imatul masud, Masud Masyitoh, Alfianita Maulidah, Nur Izzatul Moh. Sahlan Muhammad Bahrul Ulum Muhammad Irfan Mundir Mundir, Mundir Mustakim, Adam Oktafian, Akhmad Rizal Pangestika, Padhina Praciska, Intan Okta Putra, Muhammad Rizki Ana Putra, Sohnif Nurwicahyo Rahmawati, Revilda Ayu RULLY DYAH PURWATI Saihan, Saihan Sari, Aulia Ika Sintho Wahyuning Ardie Siti Zubaidah Sofyan Arief Sulistyawati Sulistyawati Supandi, Ach. Faqih TRI JOKO SANTOSO Trikoesoemaningtyas Ubaidillah Ubaidillah Ulfah, Silfiyah Untung Santoso Yahya, Syifa' Yudi Prasetyo Zakiya, Aulia