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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Dedikasi Jurnal Gamma Journal of Agroecology Jurnal Penelitian Tanaman Industri Jurnal Sain Peternakan Indonesia Kultivasi Agriprima : Journal of Applied Agricultural Sciences FALASIFA : Jurnal Studi Keislaman Islamic Management: Jurnal Manajemen Pendidikan Islam Jurnal Ilmiah Inovasi Auladuna : Jurnal Prodi Pendidikan Guru Madrasah Ibtidaiyah Jurnal Penelitian Tanaman Industri (Littri) Tafkir: Interdisciplinary Journal of Islamic Education Al-Ashr : Jurnal Pendidikan dan Pembelajaran Dasar FAJAR Jurnal Pendidikan Islam JASNA : Journal For Aswaja Studies Jurnal Dedikasi Hukum MIMBAR INTEGRITAS Jurnal Pengabdian Pada Masyarakat Jurnal AL-Muta`aliyah : Jurnal Pendidikan Guru Madrasah Ibtidaiyah Jurnal Edukasi Pengabdian Masyarakat: EDUABDIMAS EDUCARE: Journal of Primary Education Journal of Tropical Crop Science and Technology
Agus Zainudin
Universitas Muhammadiyah Malang
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Pembentukan Buah dan Perkecambahan Tanaman Jarak Pagar pada Transformasi Langsung Melalui Jalur Tabung Polen Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.449 KB) | DOI: 10.24831/jai.v46i1.12897

Abstract

The genetic transformation via pollen-tube pathway in jatropha is the first alternative method that was applied in this plant. The objective of the research was to study fruit set and germination of three genotypes of jatropha following direct transformations via pollen-tube pathways. The research was conducted during April 2014 until January 2015 at jatropa’s experimental field, Pasuruan, and at green house of ICABIOGRAD, Bogor. Three genotypes of jatropha i.e., IP3A, IP3P and JcUMM18 were used. In the first experiment, split plot design was used where 3 levels of DNA plasmid concentration as a main plot and 5 levels of stigma-drip time of DNA plasmid as a subplot. In the second experiment randomized block design was used with single factor consisting of 15 combinations of concentration and stigma-drip time of DNA plasmid as treatments and control. The results demonstrated that interaction between concentration and application time of DNA plasmid did not significantly affect fruit and seeds formation of three Jatropha genotypes. Combination of DNA plasmid concentration with time of stigma-drip had significant effect on seed germination rate of IP3A genotype, but not significant on the other variables. The concentration of 0.05-0.5 µg µL-1 and application time of DNA plasmid at 1-10 hours after pollination could be applied on jatropha genetic transformation via pollen-tube pathways.Keywords: DNA plasmid, Jatropha curcas, pCAMBIA1301, pollen-tube, stigma-drip
Efficiency of Genetic Transformation via Pollen-Tube Pathway of Jatropha (Jatropha curcas L.) Based on Histochemical and Molecular Analysis Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, and
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.424 KB) | DOI: 10.24831/jai.v45i3.12925

Abstract

The genetic transformation via pollen-tube pathway is an alternative method to overcome the constraints imposed by genotype specificity in transformation and regeneration in jatropha (Jatropha curcas L.) tissue culture. Therefore, it is necessary to establish important parameters for efficient genetic transformation of jatropha via pollen-tube pathway. The objective of the research was to study the efficiency of direct transformation of jatropha via pollen-tube pathway based on histochemical and molecular analysis. Solution of purified pCAMBIA1301 DNA plasmid carrying a hptII marker gene and a gus reporter gene with concentration level of 0.05, 0.25, 0.50 µg µl-1 were applied to stigma of flowers at 1, 2, 4, 7, 10 h after pollination. Seedling of IP3A, IP3P and JcUMM18 jatropha’s genotypes derived from 15 combination treatments of plasmid DNA concentration and application time, also wild type was subjected to histochemical and molecular analyses. Based on those analyses, the efficiency of transformation via pollen-tube pathway of three jatropha genotypes ranged from 1.5-16.7%. PCR analysis showed that a number of positive plants were identified by using specific primers hptII and gus, i.e. 1-3 and 3-7 plants of the 15 combined treatments, respectively. It indicated that the transformation efficiency via the pollen-tube pathway varied in each jatropha genotype.Keywords: Jatropha curcas L., pCAMBIA1301, plasmid DNA, stigma-drip
Metode Eksplorasi Berbasis Audio Dalam Meningkatkan Motivasi Belajar Siswa Zainudin, Agus; Yanuardianto, Elga; Efendi, Firdaus Roni
Jurnal al Muta'aliyah: Pendidikan Guru Madrasah Ibtidaiyah Vol 6 No 1 (2026): Jurnal AL-Muta`aliyah
Publisher : LP2M STAI Darul Kamal NW Kembang Kerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51700/mutaaliyah.v6i1.1381

Abstract

This study examines the implementation of the exploration method in dance arts learning, focusing on its application, obstacles, and evaluation for broader adoption across grade levels. Using a qualitative phenomenological design, the research involved purposively selected participants who possessed in-depth knowledge of the learning process. Data were collected through classroom observations, in-depth interviews, and documentation, then analyzed through data reduction, data display, and conclusion drawing. Data validity was ensured through triangulation of sources and techniques. The findings show that the exploration method effectively increased students’ enthusiasm, motivation, and active participation in dance arts learning. Students were more engaged in expressing movement ideas and responding creatively to learning activities. However, the implementation faced obstacles, particularly classroom conditions that allowed distractions from other students, which sometimes reduced learners’ concentration during activities. Limitations in classroom management and learning space also influenced the effectiveness of the method. Overall, the exploration method demonstrates positive potential for supporting student-centered learning in dance arts. To optimize its implementation, practical strategies are required, including improved classroom management and supportive learning environments. With proper adjustments, this method can be considered for wider application at various educational levels.
BIMBINGAN ANAK-ANAK MENGAJI AL QUR’AN DI YAYASAN AR ROHMAH DESA SEMBORO KIDUL KECAMATAN SEMBORO KABUPATEN JEMBER Zainudin, Agus; Nurholilah, Siti; Ernawati, Sitti; Naim, Zulfa Nadrotun; Rohana, Siti; Hidayat, Rahmad Nur; Robiussani, Muhammad; Afin, Muhammad
MIMBAR INTEGRITAS : Jurnal Pengabdian Vol 5 No 1 (2026): JANUARI 2026
Publisher : Biro Administrasi dan Akademik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/mimbarintegritas.v5i1.7699

Abstract

Pendidikan agama memiliki peran strategis dalam pembentukan karakter anak, khususnya melalui pembelajaran membaca Al-Qur’an yang benar sesuai kaidah tajwid. Yayasan Omah Yatim Ar-Rohmah Semboro sebagai lembaga pendampingan anak yatim dan dhuafa telah menjalankan program mengaji, namun masih ditemukan anak yang belum lancar membaca, belum tepat dalam mad dan tajwid, serta belum fasih melafalkan huruf hijaiyah. Pengabdian ini bertujuan meningkatkan kemampuan membaca Al-Qur’an anak secara teknis sekaligus menumbuhkan adab dan motivasi belajar. Kegiatan dilaksanakan setelah Magrib hingga menjelang Isya di Yayasan Omah Yatim Ar-Rohmah, Dusun Semboro Kidul, Desa Semboro, Kecamatan Semboro, Kabupaten Jember dengan melibatkan 25 anak usia 7–13 tahun. Metode pelaksanaan meliputi observasi awal melalui uji baca, pembelajaran bertahap (pengenalan huruf hijaiyah, latihan makharijul huruf, dan penguatan tajwid dasar), serta penerapan Metode Alimna melalui latihan terarah, pengulangan, dan pembiasaan membaca. Evaluasi dilakukan melalui tes praktik membaca surat-surat pendek dengan indikator kelancaran, ketepatan makhraj, panjang-pendek bacaan, dan penerapan tajwid. Hasil menunjukkan peningkatan signifikan pada aspek kognitif (40% menjadi 85%), afektif (50% menjadi 90%), dan psikomotorik (35% menjadi 80%). Hambatan utama berupa kurang fokus dan kedisiplinan anak dapat diatasi melalui arahan tegas, konsekuensi mendidik, serta pendampingan khusus bagi anak yang belum memahami tajwid. Program ini efektif meningkatkan kompetensi membaca Al-Qur’an sekaligus memperkuat pembiasaan adab dan karakter cinta Al-Qur’an pada anak.
Co-Authors Abdullah Adini, Almas Adlina Afin, Muhammad Ali Ikhwan Amiq, Bahrul Aniek Iriany Aris Winaya Astutik, Anik Widya Aulia Zakia BAMBANG HELIYANTO Bambang Sapta Purwoko Charismaraya, Kefassada Dian Puji Rahayu Dini Kurniawati Dyah Nawangsari Efendi, Firdaus Roni Elga Yanuardianto Erfan Dani Septia Ernawati, Sitti Fauzan, Fauzan Febriyanti, Beby Dwi HADI SUDARMO Hadi Sudarmo Haq, Farhan Chairul Helvi Ardana Reswari Hendry Sulistiyanto Hepni Hepni Hidayat, Rahmad Nur Huda, Ahmad Hisbulloh Husain, Syarief Ibrahim Ida Ayu Putu Sri Widnyani Iis Siti Aisyah Irsyad, Muammar Habibi Kholishoh, Siti Adyanur Maftuchah Maftuchah Mashudi Mashudi Masruroh, A`imatul masud, Masud Masyitoh, Alfianita Maulidah, Nur Izzatul Moh. Sahlan Muhammad Bahrul Ulum Muhammad Irfan Muhith, Abd. Mundir Mundir, Mundir Mustakim, Adam Naim, Zulfa Nadrotun Nurholilah, Siti Oktafian, Akhmad Rizal Pangestika, Padhina Praciska, Intan Okta Putra, Muhammad Rizki Ana Putra, Sohnif Nurwicahyo Rahmawati, Revilda Ayu Robiussani, Muhammad RULLY DYAH PURWATI Saihan, Saihan Sari, Aulia Ika Sintho Wahyuning Ardie Siti Rohana Siti Zubaidah Sofyan Arief Sulistyawati Sulistyawati Supandi, Ach. Faqih TRI JOKO SANTOSO Trikoesoemaningtyas Ubaidillah Ubaidillah Ulfah, Silfiyah Untung Santoso Yahya, Syifa' Yudi Prasetyo Zakiya, Aulia