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Journal : AGRIVITA, Journal of Agricultural Science

Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability
Sensitivity in Callus Tissue of Plectranthus amboinicus (L.) through Mutation Induction with Colchicine Kunto Wibisono; Syarifah Iis Aisyah; Waras Nurcholis; Sri Suhesti
AGRIVITA, Journal of Agricultural Science Vol 44, No 1 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i1.3058

Abstract

Mutation induction by colchicine is one method to improve plant genetic diversity (polyploidy). This study aims to 1) obtain growth regulators composition suitable in inducing callus, 2) obtain the LC50 value in callus of P. amboinicus, 3) to determine the effect of colchicine on callus growth, 4) to get shoots (putative mutant) varian, regenerating from indirect organogenesis (callus) in MV1. The study is conducted at the Laboratory of Agricultural Seed Management, Plantation Research, and Development Center from June 2018 to June 2019. The study uses a complete randomized design with four treatments and three replications. Explants use ware the nodes. Callus induction uses MS0 medium with 0.5 ppm 2.4-D, 2 ppm 2.4-D, 0.5 ppm 2.4-D + 1 ppm BAP, and 0.5 ppm 2.4-D + 0.5 ppm TDZ treatment. Mutation induction was performed using colchicine concentration at 0%, 0.02%, 0.04%, and 0.06% treatments. The regeneration media is an MS0 medium. The results show the best combination of plant growth regulators to induce the callus is 0.5 ppm 2.4-D. The LC50 colchicine concentration is at 0.0275%. However, this phenotype is reversible. An additional subculture period is required to move to normal morphology
The Sensitivity of Four Garlic Genotypes on Low Temperatures and the Role on Dormancy Breaking Niken Kendarini; Syarifah Iis Aisyah; Awang Maharijaya; Sobir Sobir
AGRIVITA Journal of Agricultural Science Vol 45, No 3 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i3.4177

Abstract

The garlic seed bulbs cannot be planted immediately after harvest because has dormant period. Bulb dormancy can be broken by exposure of pre-planting bulbs to low temperatures. The research aimed to determine the sensitivity of different bolting types of garlic genotypes at low temperatures and the role of low temperatures on dormancy break. The experiment was conducted from March to June 2019 at the Universitas Brawijaya, using a nested design with three replications. Chinese hardneck and softneck, Sangga Sembalun and Tawangmangu Baru were the garlic accessions used in the study. The storage temperature treatments at 3 and 7°C; and room temperature at 27°C. Chinese softneck bulb had the highest sprouting and rooting after 3 and 7°C storage and was sensitive to low temperatures. The Chinese hardneck had the lowest sprouting and was highly insensitive to cold stress. Sangga Sembalun and Tawangmangu Baru had sprouted bulbs in between these Chinese genotypes, and they were insensitive and highly insensitive to low temperatures, respectively. Metabolites of 5-hydroxymethyl-2-furancarboxaldehyde, palmitic acid, diallyl trisulfide, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one played important roles in the bulb response to low temperature stress and directly or indirectly involved in the sprouting and rooting in dormant garlic cloves.
Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.76

Abstract

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability
Co-Authors , Krisantini Abdul Hakim Agus Purwito Arum Sholikhah ASEP SAEFUDDIN Awang Maharijaya Aziz, Sandra A Azri Kusuma Dewi Azzahra, Elmi Irmayanti Bagas Akmala Putra Budi Marwoto Dewa Ngurah Suprapta Dewi Sukma Diny Dinarti Endang Gati Lestari Endang Gati Lestari Eny Rolenti Togatorop Fahmi Muhammad Cokrosudibyo Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hasna Dama Hayati, Dhieni Helfi Eka Saputra Helfi Eka Saputra Ika Roostika Joko Ridho Witono Kartiman, Roni Kartiman, Roni Kunto Wibisono Kunto Wibisono Kunto Wibisono Kurniati, Ridho Latifah K Darusman Mariana Susilowati, Mariana Marthin, Yodi Marwoto, Budi Masna Maya SINTA Monica Christina Natalia Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Riyo Agung Kurnia Muhammad Syukur Mulyana, Erik Muthi'ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Natalia, Monica Christina Nella Angelina Simanjuntak Ni Made Armini Wiendi Niken Kendarini Nurgravisi, Primanisa Nurul Komala Qisthi Kustia Rahman RAGAPADMI PURNAMANINGSIH Rahman, Qisthi Kustia RAHMI YUNIANTI Rapi, Muh. Nur Alif Rara Annisaur Rosyidah Ritonga, Arya Widura Riyan Alifbi Putera Irsal ROHIM FIRDAUS Rohim Firdaus Roni Kartiman Roni Kartiman Rosa Yunita Rosa Yunita Rusmiyati, Henny S Sudarsono Sari, Dia Novita Sarsidi Sastrosumarjo Sembiring, Rinawati Setiawan, Farensa Ikman Dedi Siti Hartati Yusida Saragih Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir, dan Soeranto Human Sri Suhesti Sri Suhesti Sri Suhesti Sri Wahyuni Sukma, Dewi Sulassih, . Supijatno Suryawati, nFN Tamami, Djoko Theresia Dame Angel Lie Manurung Trikoesoemaningtyas Waras Nurcholis Wijaya Murti Indriatama Wijaya Murti Indriatama Winarni, Utin Wulan Septiningtyas Kurniajati yahidah Rosyidah Anshori Yasmin, Zahra Fadhlia Yoshua Shandy Yudha Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yustia Yulianti Zahra Fadhlia Yasmin