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All Journal International Journal of Public Health Science (IJPHS) Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Majalah Obat Tradisional Jurnal Farmasi Sains dan Komunitas Jurnal Surya Medika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Ilmu Kefarmasian Indonesia JURNAL FARMASIMED (JFM) Majalah Farmasetika CERATA Jurnal Ilmu Farmasi Indonesian Journal of Halal Research Majalah Ilmiah Warta Dharmawangsa JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Farmasi Sains dan Praktis Journal of Halal Science and Research Jurnal Inovasi Pengabdian dan Pemberdayaan Masyarakat CERATA Medical Sains : Jurnal Ilmiah Kefarmasian

Hari Susanti

Universitas Ahmad Dahlan

Author-ID : 2411629

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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Perbandingan Metode Spektrofotometri UV Dan HPLC pada Penetapan Kadar Kafein dalam Kopi Hari Susanti; Nisa Putri Mujaadillah Araaf; Aprilia Kusbandari
Majalah Farmasetika Vol. 4, Supl. 1, Tahun 2019
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mfarmasetika.v4i0.25887

Kafein merupakan salah satu alkaloid yang terkandung dalam kopi. Konsumsi kafein dalam jumlah besar bisa berdampak pada kesehatan manusia. Kebiasaan orang-orang jamun dulu minum kopi tradisional, maupun orang jaman sekarang minum kopi dengan berbagai varian tentu akan berpengaruh pada kondisi kesehatan. Sehingga perlu diketahui kandungan kafein dalam kopi. Peneliti terdahulu telah melakukan penetapan kadar kopi dengan metode HPLC maupun Spektrofotometri. Namun belum ada yang membandingkan kedua metode. Penelitian ini bertujuan untuk membandingkan metode spektrofotometri UV dan HPLC dalam penetapan kadar kafein dalam kopi. Sampel yang digunakan adalah sediaan kopi hijau dan kopi hitam. Perbandingan metode dilakukan dengan membandingkan data hasil validasi metode antara spektrofotometri UV dan HPLC. Metode spektrofotometri dilakukan dengan pelarut air, absorbansi kafein dibaca pada panjang gelombang 272 nm. Metode HPLC dilakukan dengan fase diam C18, fase gerak matanol-air (69:40 v/v) flow rate 1 ml/menit dan detector UV 272 nm. Hasil validasi metode analisis kafein secara spektrofotometri adalah sebagai berikut : linearitas (R= 0,9965), presisi (RSD= 0,899%), akurasi (recovery = 98,46%) LOD= 1,12 ug/ml, LOQ=3,75 ug/ml. Hasil validasi metode HPLC adalah: linearitas (R= 0,998), Presisi (RSD= 2,49%), akurasi (Recovery= 84%), LOD=0,565 ug/ml, LOQ= 1,88 ug/ml. Kadar kafein dalam sampel kopi hijau sediaan dan kopi hitam sediaan, secara Spektrofotometri UV bertrurut-turut (dalam %) adalah : (0,155± 0,053) dan (0,696 ± 0,014). Kadar kafein dalam kopi hijau sediaan, kopi hitam sediaan, secara HPLC berturut-turut (dalam %) adalah ; 0,121 dan 0,421. Metode spektrofotometri UV direkomendasikan sebagai metode pilihan pada penetapan kadar kafein dalam sampel kopi.

Aktivitas Antioksidan dan Nilai SPF (Sun Protection Factor) Ekstrak Etanol Buah Pepaya (Carica papaya L.) yang Diperoleh dari Simplisia dan Buah Segar Aditya Noviadi Rakhmatullah; Nining Sugihartini; Hari Susanti
Jurnal Surya Medika (JSM) Vol 5 No 2 (2020): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Papaya contains vitamin C and beta-carotene which are useful as antioxidants and can be used as a sunscreen. The content of the active substance is affected by the type of raw material. This study aims to determine the antioxidant activity and SPF value of extracts obtained from simplicia and fresh fruit. Extracts obtained from simplicia and fresh fruit were determined antioxidant activity by DPPH method and SPF values by spectrophotometric method. The results showed that the antioxidant activity of papaya extract obtained from simplicia had IC50 values smaller than papaya extract obtained from fresh fruit, whereas the papaya extract obtained from simplicia had IC50 values of 175 μg / mL and the papaya extract obtained from fruit fresh is 209 μg / mL. While in the sunscreen test, the SPF value of papaya extract obtained from simplicia is greater than the papaya extract obtained from fresh fruit. SPF value of papaya extract obtained from simplicia is 37, while papaya extract obtained from fresh fruit is 35. The conclusion from this study is papaya extract extracted by the drying process of the initial material (simplicia) has antioxidant activity and SPF values that are more optimal than papaya extracts extracted without going through a drying process first (fresh fruit).

Pengaruh Purifikasi Terhadap Kandungan Zat Aktif Dan Aktivitas Antioksidan Ekstrak Etanol 50% Daun Kelor (Moringa oleifera L.) Diyan Sakti Purwanto; Hari Susanti; Nining Sugihartini
CERATA Jurnal Ilmu Farmasi Vol 12 No 2 (2021): Cerata Jurnal Ilmu Farmasi
Publisher : STIKES MUHAMMADIYAH KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Purifikasi ekstrak etanol 50% daun kelor (Moringa oleifera L.) dilakukan untuk meningkatkan kandungan zat aktif flavanoid, β-karoten, saponin dan tanin yang memiliki aktivitas sebagai antioksidan. Tujuan penelitian ini adalah mengetahui jenis pelarut yang paling optimal antara etil asetat dan n-heksan dalam fraksinasi ekstrak etanol 50% daun kelor. Penelitian dimulai ekstraksi etanol 50% daun kelor dengan maserasi dan dilanjutkan evaporator kemudian waterbath sampai diperoleh ekstrak kental. Ekstrak kental yang diperoleh dari ekstraksi selanjutnya dilakukan purifikasi. Fraksinasi dengan melarutkan ekstrak kental etanol 50% daun kelor (E1) dan aquades pada suhu 70ºC kemudian ditambahkan etil asetat pada corong pisah hingga di peroleh fraksi etil asetat (E2), ekstrak kental etanol 50% daun kelor (E1) ditambahkan n-heksan pada corong pisah hingga terjadi pemisahan fase sehingga diperoleh fraksi n-heksana (E3). Ekstrak dan fraksi dievaluasi parameter meliputi kadar air ekstrak dengan gravimetri, kadar β-karoten dengan HPLC dan aktivitas antioksidan dengan DPPH. Data dianalisis statistik dengan taraf kepercayaam 95%. Hasil Penelitian menunjukkan bahwa kadar air ekstrak etanol 50% daun kelor adalah 4,16 dengan CV 4,62%. Kadar β-karoten paling optimal pada fraksi n-heksana (E3) kemudian etil asetat (E2) dan etanol 50% (E1) yaitu 1.48 ± 0.01%, 1.19 ± 005% dan 0.73 ± 0.01%. Sedangkan aktivitas antioksidan (IC50) paling optimal pada fraksi n-heksana (E3) kemudian etanol 50% (E1) dan etil asetat (E2) yaitu 40.83 ± 0.04 ug/ml, 47.75 ± 0.09 ug/ml dan 58.79 ± 0.10ug/ml. Nilai aktivitas antioksian menunjukkan pada fraksi n-heksana dan ekstrak etanol 50% termasuk dalam kategori sangat kuat sedangkan fraksi etil asetat termasuk dalam kategori kuat sehingga berpotensi sebagai antioksidan.

Determination of epms content and anti-inflammatory test rhizome extract Kaempferia galanga, L by inhibition of protein denaturation method Laela Hayu Nurani; Aris Asahi; Hari Susanti
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Kencur (Kempferia galanga, L) is one of the rhizomes that is often used as a constituent component in traditional medicinal formulas. One of the kencur's pharmacological activities is anti-inflammatory. The active compound as an anti-inflammatory is ethyl p-methoxycinnamate (EPMC). This study aims to determine the content and ascertainment of EPMC compounds and the anti-inflammatory activity of kencur rhizome extract. Kencur extract can be obtained by the maceration method using 96% ethanol solvent. The EPMC content determination was held using the TLC-densitometry method, while the EPMC compounds were confirmed using the GC MS method. The anti-inflammatory activity test was done using inhibiting protein denaturation methods. The extract results obtained 12.67% yield. The result of EPMC content in kencur extract was 10.05 Â± 4.57. The GCMS kencur extract results showed an abundance of EPMC compounds at a retention time of 7.088 with a peak area of 72290779. The results of IC50 anti-inflammatory for the standard substance (EPMC) were 5.306 Â± 5.028. The results of IC50 anti-inflammatory the sample (kencur extract) was 303.487 Â± 1.201. Ethanol extract of kencur contains ethyl p-methoxycinnamate (EPMC) and has anti-inflammatory activity by inhibiting protein denaturation.Â

PENGHAMBATAN AKTIVITAS XANTHINE OXIDASE OLEH EKSTRAK ETANOL AKAR SAMBILOTO (Andrographis paniculata, Ness) SECARA IN VITRO Ulfah Septianingsih; Hari Susanti; Wahyu Widyaningsih
Pharmaciana Vol 2, No 2 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Sambiloto root of which contained of flavonoids used by the people fortraditional medicine. In the previous publication, an effective xanthine oxidaseinhibitory activity of flavonoids was reported. In research, in vitro xanthine oxidaseinhibitory activity of etanolic root extract of Andrographis paniculata was determinedand Allopurinol was used as a control. The etanolic extract was succesively extractedin a Soxhlet with petroleum eter. Inhibition of xanthine oxidase by etanolic extract wasmeasured the decrease of uric acid production and monitored by spectrophotometer at295 nm with xanthine as substrat. The enzyme inhibitory activity was calculated, andthen IC50 was determined. The result of analyzed with Kruskal Wallis and MannWhitney at 95% confidental level . The flavonoids of etanolic extract were separated onusing by paper chromatography and the spot changing was determined using UV 366with and without amonia.The result of the research showed that the etanolic extract ofAndrographis paniculata inhibited xanthine oxidase activity with IC50 16,54 Âµg/mlwhile Allopurinol 4,29 Âµg/ml. The etanolic extract contained flavon or flavonol.

UJI EFEK ANTIHIPERGLIKEMIK EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta, Merr) PADA TIKUS PUTIH JANTAN GALUR WISTAR Farida Baroroh; Nurfina Aznam; Hari Susanti
Pharmaciana Vol 1, No 1 (2011): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Kaca piring leaf (Gardenia augusta Merr.) was often used in traditional drugs fortreatment diabetes mellitus. This research aim to know the antihiperglycemic effectethanolic extract of kacapiring leaf on white rats male strain of Wistar and how muchantihiperglycemic effect compared with glibenklamid. This study used oral glucosetolerance test method with loading glucose 4,5 g/kgW. Tested animal where 24 whitemale rats strain of Wistar age 2-3 months with weight 180-250 gram, devided into 4groups, each groups consist of 6 rats. Group I was as negative control group givenCMC-Na 1%, group II was as positive control group given glibenklamid dose 1,35mg/kgBB, group III and IV where given ethanolic extract of kacapiring leaf dose 500mg/kgBB and 250 mg/kgBB. Glibenklamid and exstract are given orally 60 minutesbefore glucose. Blood was taken from orbitalis sinus at minute (-90), (-60), 0, 30, 60,120, 180, 240, and 300. Blood glucose level was determined with GOD PAP enzymaticmethod, absorbance was observed using spectrophotometer at 500 nm. The result of the study was performed by giving ethanolic extract of kacapiring leaf dose 500 mg/kgW and 250 mg/kgW had antihiperglycemic effect. Ethanolic extract of kacapiring leaf dose500 mg/kgW and 250 mg/kgW could reduce blood glucoce level 58,97% and 80,60% compared with glibenklamid dose 1,35 mg/kgW could reduce blood glucoce level73,93%.

PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KELOPAK BUNGA ROSELLA MERAH (Hibiscus sabdariffa Linn) DENGAN VARIASI TEMPAT TUMBUH SECARA SPEKTROFOTOMETRI Riza Alfian; Hari Susanti
Pharmaciana Vol 2, No 1 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The purpose of this research is to determine the total phenolic content of Hibiscussabdariffa calyx in variations of growing area. Red Rosell calyxs were collected fromGlagah, Kediri, and Samigaluh. Phenolic compounds of Hibiscus sabdariffa calyx wereextracted using maceration method with methanol. Total phenolics content weredetermined using visible spectrophotometry method with Folin Ciocalteau reagent. Theprinciple of this method is the formation of blue complex compound fromphospomolybdate-phosphotungstate reduced by phenolic compound in the basiccondition, which can be measured by spectrophotometry. Galic acid was used ascomperator in this research. Total phenolic content in red calyx Glagah, Kediri andSamigaluh were respectively 1.40 g GAE/100 g extract, SD 0.06 (n=12), 1.41 gGAE/100 g extract, SD 0.07 (n=12) dan 2.12 g GAE/100 g extract, SD 0.05 (n=15).Based on this results it could be concluded that growing area affected total phenoliccontent in the methanol extract of red calyx Rosell.

PENGHAMBATAN AKTIVITAS XANTHINE OXIDASE OLEH EKSTRAK ETANOL SARANG SEMUT (Myrmecodia tuberosa (non Jack) Bl.) SECARA IN VITRO Ernawati Ernawati; Hari Susanti
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Uric acid is the end product of purine metabolism that can settle in the joints and form smallcrystals, causing inflammation known as gout. Gout is a disease caused by high levels of uric acid inthe blood (hyperuricemia). One of the drugs used to treat gout was allopurinol to inhibite the activityof xanthine oxidase mechanism. Myrmecodia tuberosa (non Jack) Bl. known to contain flavonoids andempirically proven to treat rheumatic complaints and gout. Therefore, research needs to examinewhether the ethanol extract of the sarang semut to inhibite xanthine oxidase activity. Allopurinol isused as a comparison. The active substances of the Myrmecodia tuberosa (non Jack) Bl. was extractedwith ethanol using maceration method after soaked with petroleum ether. The xanthine oxidaseinhibitor activity of the Myrmecodia tuberosa (non Jack) Bl production ethanol extract wasspectrophotometrically determined by monitoring the reducing of uric acid at a wavelength(Î») 295 nm with xanthine as substrate. Rate values obtained subsequently used to calculate the valueof the activity. Then determined the concentration of the ethanol extract can inhibit the activity ofof xanthine oxidase by 50% (IC50). The results of the research showed that the ethanol extracts of sarang semut inhibited xanthine oxidase activity with IC50 112.40 Âµg/ml, while allopurinol was3.16 Âµg/ml.

EFEK ANTIANGIOGENESI EKSTRAK ETANOL GANGGANG HIJAU (Spirogyra sp.) BERDASARKAN EKSPRESI COX-2 PADA SEL T47D Wahyu Widyaningsih; Nina Salamah; Hari Susanti; Dwi Fitriani
Majalah Obat Tradisional Vol 19, No 3 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Cancer is a group of diseases that arise when a cell or group of cells that regulate out of control growth. Green algae (Spirogyra sp.) is one of the medicinal plants used in traditional medicine for the treatment of cancer. Green algae (Spirogyra sp.) has active substances such as melatonin. Melatonin which is a compound that has been examined by researchers world as anticancer drugs and antioxidants. This study aims to determine the effect of ethanol extract of green algae (Spirogyra sp.) on the expression of COX - 2 in T47D cells. Green algae was extracted using a Soxhlet apparatus with 96 % ethanol. This study used three groups: control cells , ethanol extract of green algae concentrations 247.668 ug / mL and 123.834 mg / mL. To ensure the expression of caspase-3 and Bcl-2, test was conducted using indirect immunocytochemistry. Observations were carried out using a light microscope to see and count the percent expression of COX - 2. The results obtained showed significant difference, decreased expression of COX - 2 in each group. It can be concluded that the ethanol extract of green algae (Spirogyra sp.) decreased COX - 2 expression in T47D cells.

PENETAPAN KADAR DIOSGENIN DALAM EKSTRAK AIR Costus speciosus SECARA HPLC Hari Susanti; Subagus Wahyuono; Ratna Asmah Susidarti; Ika Puspita Sari
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Costus speciosus has been reported to have biological activity among antidiabetic, antihyperlipidemia, antioxidants. One of the chemical constituents that is responsible for some of the biological activity is diosgenin. This study aimed to determine diosgenin content of Costus speciosus water extract (CS). Water extract of CS was obtained with infundation method. The extract was dried using freeze dryer. The assay of diosgenin was performed by HPLC method. HPLC system used: LiChrospher® 100 RP-18 endcapped (5 µm) column length 25cm, colimn id 4 mm as stationary phase, acetonitrile-water (9: 1 v/v) as mobile phase, flow rate 2mL/min and UV detection at 205 nm. HPLC method developed was linear in the range 20-200 μg mL with R = 0.9999 ; slope = 6423.7; and intercept = -4280.5; LOD = 2.14 ug/mL and LOQ = 6.50 mg/mL; and recovery 100.63 %. Diosgenin levels in CS was 0.279 %. The developed HPLC method is relatively simple, rapid, sensitive, and accurate to determine the content of diosgenin in water extracts of Costus speciosus.

Co-Authors Aditya Noviadi Rakhmatullah Afriyani, Neti Ajeng Dian Pertiwi, Ajeng Dian Alfian, Riza Anggita Devi Anita Wening Sejati Any Guntarti Any Guntarti Aprilia Kusbandari, Aprilia Aris Asahi Baroroh, Farida Chotimah, Aditya Nurul Desyandri Desyandri Diyan Sakti Purwanto Dwi Fitriani Dwi Fitriani Ernawati Ernawati Ernawati, Ernawati Farida Baroroh Faridah Baroroh Hana Hanifah Hasanul Kiyan Al-kayyis Heldin, Siti Salamiah Putri Ika Puspita Sari INNAYAH IZATI Jaswir, Irwandi Jufri, Sayyidah Luthfiyah Laela Hayu Nurani Mohamed, Farahidah Nina Salamah Nining Sugihartini Nisa Putri Mujaadillah Araaf Nurfina Aznam Pratiwi, Tiara Bella Puput Herawati Said Hasan Rahayu, Titi Pudji Rahmat Hidayat Ratna Asmah Susidarti Ratna Yuningtyas Riza Alfian Rozak, Miftahul Santoso, Blegoh Iwan Sapto Yuliani Septianingsih, Ulfah Siti Ayu Marlina Siti Rohmah Subagus Wahyuono Sultan, Siti Fatimah Supriyanto, Sugeng Tri Octaviani Ulfah Septianingsih Ulfah Septianingsih Verda Farida Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Yogo Dwi Prasetyo Yuningtyas, Ratna Zaenab Zaenab Zahra Alya Putri Zainab Zainab Zainab Zainab

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