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PERBANDINGAN METODE SOMOGYI-NELSON DAN ANTHRONE-SULFAT PADA PENETAPAN KADAR GULA PEREDUKSI DALAM UMBI CILEMBU (Ipomea batatas L.) Hasanul Kiyan Al-kayyis; Hari Susanti
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 13, No 2 (2016)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.425 KB) | DOI: 10.24071/jpsc.00191

Abstract

Sweet potato cultivar Cilembu, kind of rancing from Cilembu Village, Sumedang, West Java has really sweet taste due to the high content of reducing sugar. The amount of reducing sugar needs to be proven scientifically. The study aimed to compare Somogyi-Nelson method and Anthrone-Sulfate method in order to determine reducing sugar level in Cilembu sweet potato. Cilembu sweet potato must be stored in freezer at 15C for 2 weeks before used as the sample. Analytical method validation was performed for the two methods. The determination results between two methods were compared. The amount of reduction sugar (glucose) from Cilembu sweet potato determined by Somogyi-Nelson method was 3.42%, the value of %recovery was 99.8%, LOD = 0.00167 (mg/mL), LOQ = 0.00557 (mg/mL), and value of RSD was 3.7%. While the average amount of reduction sugar determined by Anthrone-Sulfate method was 14.9%, the value of %recovery was 74.7%, LOD = 0.0198 (mg/mL), LOQ = 0.066 (mg/mL), and value of RSD = 8.6%. The Somogyi-Nelson method was more recommended for the analysis of the reducing sugar in Cilembu sweet potato compared with Anthrone-Sulfate method.
STABILITAS EPIGALOKATEKIN GALAT DALAM KRIM EKSTRAK TEH HIJAU DENGAN VARIASI KONSENTRASI ANTIOKSIDAN VITAMIN C 1% DAN VITAMIN E 1% Nining Sugihartini; Hari Susanti; Zaenab Zaenab; Hana Hanifah; Siti Ayu Marlina
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 13, No 2 (2016)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.558 KB) | DOI: 10.24071/jpsc.00186

Abstract

Epigallocatechingallate (EGCG) in green tea extract has activity as an anti-inflammatory agent. On the other hand the stability of EGCG is poor because of the oxidation. The aim of this study was to determine the influence of Vitamine C and Vitamine E in formulation of green tea extract cream to the stabiliy of EGCG. The green tea extract was obtained from the extraction process by infundation followed by fractination with ethyl acetate as the solvent. The three formulas were compiled in similar composition with the concentration of vitamine C 1% (FI), Vitamine E 1% (FII) and there was no Vitamine C and Vitamine E (FIII) as a control. The EGCG level was determinated by TLC-densitometry methode. The stability parameter was determinated by calculated of the Q10 of each formula. The result of this study showed that the parameter of t90 of EGCG with Vitamine C 1%, Vitamine E 1% and control addition were 0.0108 hours, 0.0087 hours, 0.0084 hours, respectively. Stability of EGCG in green tea leaf extract cream with addition of the vitamin C 1% was higher than it stability with the addition of vitamin E 1%. The concentration of Vitamin C 1% was the optimum concentration as antioxidant in formulation of green tea extract cream.
Free Radical Scavenger Activity of Green Algae Ethanolic Extract Spirogyra sp. and Ulva lactuca Using DPPH Method NINA SALAMAH; WAHYU WIDYANINGSIH; INNAYAH IZATI; HARI SUSANTI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.547 KB)

Abstract

Antioksidan merupakan senyawa yang dapat menghambat reaksi oksidasi dengan mengikat radikal bebas dan molekul yang sangat reaktif. Spirogyra sp. merupakan contoh spesies yang hidup di perairan tawar, sedangkan Ulva lactuca di perairan laut. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan sebagai penangkap radikal bebas ekstrak etanol ganggang hijau Spirogyra sp. dan Ulva lactuca dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Ekstrak etanol serbuk ganggang hijau Spirogyra sp. dan Ulva lactuca dibuat dengan dengan metode maserasi. Uji aktivitas antioksidan dilakukan dengan metode DPPH secara spektrofotometri visibel. Asam galat digunakan sebagai kontrol positif. Aktivitas penangkapan radikal bebas dinyatakan dalam ES50. Hasil penelitian menunjukkan bahwa ekstrak etanol Spirogyra sp. dan Ulva lactuca memiliki aktivitas sebagai penangkap radikal bebas. Nilai ES50 Spirogyra sp. sebesar (92,83±0,58) μg/mL dan Ulva lactuca sebesar (1376,65±7,80) μg/mL, sedangkan asam galat memiliki harga ES50 sebesar (0,73 ± 0,04) μg/mL
Total phenolic content and antioxidant activities of binahong (Anredera cordifolia.) Hari Susanti
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 2, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/10.20885/JKKI.Vol10.Iss2.art9

Abstract

Background: Binahong plants are reportedly containing polyphenols, flavonoids, and steroid compounds. The content of polyphenols and flavonoids plays an important role as antioxidants. Research using the method of FRAP (Ferric Reducing Ability of Power) indicates that the antioxidant content of the binahongleaves extract is 3.68 mmol/100 g in dried leaves. This study offers new proof of antioxidant activity of all the aerial part of binahong.Objective: This research aims to determine the total phenolic content from binahong extract and its ability as free radical scavenger using DPPH method.Methods: The plant used in this research is every part of plants on the ground. The binahong extract is obtained by a maceration method with various solvents (hexane, chloroform, and methanol). Total phenolic content in the extract is spectrophotometrically determined with the Folin Ciocalteu reagent. Antioxidant activity assay is performed in vitro by the method of DPPH, with Gallic acid as a comparator. ES50 parameter is determined from the linear regression equation between the concentration of extracts and % scavenging.Results: The results showed that the total phenolic content of hexane, chloroform and methanol extract of Binahong was 8.54 ± 0.49 GAE mg/g, 17.30 ± 0.47 GAE mg/g and 32.5 ± 1.11 GAE mg/g. Hexane extract, chloroform extract and methanol extract of Binahong have a free radical scavenger activity. The value of ES50 extracts of hexane, chloroform, methanol, and successive acid errors is 58.601 ± 2.533 µg/ml, 446.219 ± 2.268 µg/ml, 237.683 ± 13.373 µg/ml and 2.058 ± 0.002 µg/ml.Conclusion: The capability of the three of binahong extract as a free radicals DPPH scavenger were weaker than gallic acid.Background: Binahong plants are reportedly containing polyphenols, flavonoids, and steroid compounds. The content of polyphenols and flavonoids plays an important role as antioxidants. Research using the method of FRAP (Ferric Reducing Ability of Power) indicates that the antioxidant content of the binahongleaves extract is 3.68 mmol/100 g in dried leaves. This study offers new proof of antioxidant activity of all the aerial part of binahong.Objective: This research aims to determine the total phenolic content from binahong extract and its ability as free radical scavenger using DPPH method.Methods: The plant used in this research is every part of plants on the ground. The binahong extract is obtained by a maceration method with various solvents (hexane, chloroform, and methanol). Total phenolic content in the extract is spectrophotometrically determined with the Folin Ciocalteu reagent. Antioxidant activity assay is performed in vitro by the method of DPPH, with Gallic acid as a comparator. ES50 parameter is determined from the linear regression equation between the concentration of extracts and % scavenging.Results: The results showed that the total phenolic content of hexane, chloroform and methanol extract of Binahong was 8.54 ± 0.49 GAE mg/g, 17.30 ± 0.47 GAE mg/g and 32.5 ± 1.11 GAE mg/g. Hexane extract, chloroform extract and methanol extract of Binahong have a free radical scavenger activity. The value of ES50 extracts of hexane, chloroform, methanol, and successive acid errors is 58.601 ± 2.533 µg/ml, 446.219 ± 2.268 µg/ml, 237.683 ± 13.373 µg/ml and 2.058 ± 0.002 µg/ml.Conclusion: The capability of the three of binahong extract as a free radicals DPPH scavenger were weaker than gallic acid.
ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (BRASSICA OLERACEA L. VAR. CAPITATA F. RUBRA) AND WHITE CABBAGE (BRASSICA OLERACEA L. VAR. CAPITATA F. ALBA) ETHANOL EXTRACT USING DPPH METHOD (1,1-DIFENIL-2-PIKRILHIDRAZIL) Any Guntarti; Ratna Yuningtyas; Hari Susanti; Zainab Zainab
Jurnal Farmasi Sains dan Praktis Vol 7 No 2 (2021)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v7i2.4369

Abstract

Antioxidants are compounds that are needed by the body to protect the body from free radical attacks that can trigger the emergence of degenerative diseases. One of the compounds that has free radical scavenger activity by inhibiting Reactive Oxygen Species (ROS) is flavonoids found in purple cabbage and white cabbage. This study aims to determine the total levels of flavonoids expressed as quercetin equivalent (EK) and antioxidant activity using the DPPH method (1,1-diphenyl-2- picrylhydrazyl). Identification of flavonoid compounds by Willstater test and determination of total flavonoid levels using spectrophotometry, AlCl3 reagent. Flavonoid levels were calculated using linear regression equations. Qualitative test for the presence of antioxidant activity using TLC and antioxidant activity test using the DPPH method with the parameter value of ES50. The qualitative test results showed that the ethanol extract of purple cabbage and white cabbage contained flavonoids. The total flavonoid content of ethanol extract of purple cabbage was 5.17% w/w (EK) and ethanol extract of white cabbage was 3.84% w/w (EK). Qualitative test with TLC showed antioxidant activity. The standard antioxidant activity values ​​of quercetin, ethanol extract of purple cabbage and white cabbage were 2.138±0.064 µg/mL; 154.445±0,999 µg/mL and 373.546±1.336 µg/mL. The antioxidant activity of purple cabbage is weak and white cabbage is very weak.
The Comparison of Analytical Methods for Determination of Eugenol Content in Water Soluble Base from Clove Flower (Syzygium aromaticum) Essential Oil Ointment using UV-Vis Spectrophotometry and High Performance Liquid Chromatography Puput Herawati Said Hasan; Nining Sugihartini; Hari Susanti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4522

Abstract

The eugenol content in clove essential oil can be used as a parameter to determine the chemical stability of clove essential oil ointment. Both UV- Vis and HPLC spectrophotometry methods can be used to determine eugenol levels. However, no studies have compared the two methods. This study aims to find out which analytical method is more valid in determining the eugenol content of clove flower essential oil (Syzygium aromaticum) in water-sea based ointments, between the UV-Vis spectrophotometric method and the HPLC method. The parameters in the validation of the analytical method carried out include parameters of linearity, selectivity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The results of method validation using UV spectrophotometry showed linearity (R = 0.9998), precision (CV = 0.137%), LOD 0.190 µg/mL, LOQ 0.632µg/mL, accuracy (recovery = 100.05%). Method validation using HPLC produced parameters of linearity (R= 0.998), precision (CV = 0.533%), LOD 0.28 µg/mL, LOQ 0.93 µg/mL, accuracy (recovery = 106.43. Average levels of eugenol in water-soluble base MABC ointment using UV spectrophotometry (% concentration = 2.201 ± 0.037) while using HPLC (% concentration = 3.191 ± 0.023). Both methods met the validation requirements. The assay results using both methods met the requirements according to the Indonesian Pharmacopoeia Edition VI. The two methods did not have a significant difference based on the student test, both in the validation parameters and the results of determining the levels of eugenol.
Pengaruh Purifikasi Terhadap Kandungan Zat Aktif Dan Aktivitas Antioksidan Ekstrak Etanol 50% Daun Kelor (Moringa oleifera L.) Diyan Sakti Purwanto; Hari Susanti; Nining Sugihartini
CERATA Jurnal Ilmu Farmasi Vol 12 No 2 (2021): Cerata Jurnal Ilmu Farmasi
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/cerata.v12i2.196

Abstract

Purifikasi ekstrak etanol 50% daun kelor (Moringa oleifera L.) dilakukan untuk meningkatkan kandungan zat aktif flavanoid, β-karoten, saponin dan tanin yang memiliki aktivitas sebagai antioksidan. Tujuan penelitian ini adalah mengetahui jenis pelarut yang paling optimal antara etil asetat dan n-heksan dalam fraksinasi ekstrak etanol 50% daun kelor. Penelitian dimulai ekstraksi etanol 50% daun kelor dengan maserasi dan dilanjutkan evaporator kemudian waterbath sampai diperoleh ekstrak kental. Ekstrak kental yang diperoleh dari ekstraksi selanjutnya dilakukan purifikasi. Fraksinasi dengan melarutkan ekstrak kental etanol 50% daun kelor (E1) dan aquades pada suhu 70ºC kemudian ditambahkan etil asetat pada corong pisah hingga di peroleh fraksi etil asetat (E2), ekstrak kental etanol 50% daun kelor (E1) ditambahkan n-heksan pada corong pisah hingga terjadi pemisahan fase sehingga diperoleh fraksi n-heksana (E3). Ekstrak dan fraksi dievaluasi parameter meliputi kadar air ekstrak dengan gravimetri, kadar β-karoten dengan HPLC dan aktivitas antioksidan dengan DPPH. Data dianalisis statistik dengan taraf kepercayaam 95%. Hasil Penelitian menunjukkan bahwa kadar air ekstrak etanol 50% daun kelor adalah 4,16 dengan CV 4,62%. Kadar β-karoten paling optimal pada fraksi n-heksana (E3) kemudian etil asetat (E2) dan etanol 50% (E1) yaitu 1.48 ± 0.01%, 1.19 ± 005% dan 0.73 ± 0.01%. Sedangkan aktivitas antioksidan (IC50) paling optimal pada fraksi n-heksana (E3) kemudian etanol 50% (E1) dan etil asetat (E2) yaitu 40.83 ± 0.04 ug/ml, 47.75 ± 0.09 ug/ml dan 58.79 ± 0.10ug/ml. Nilai aktivitas antioksian menunjukkan pada fraksi n-heksana dan ekstrak etanol 50% termasuk dalam kategori sangat kuat sedangkan fraksi etil asetat termasuk dalam kategori kuat sehingga berpotensi sebagai antioksidan.
FORMULASI SEDIAAN FACE WASH EKSTRAK DAUN KELOR (Moringa oleifera L.) DAN EKSTRAK BIJI KOPI ROBUSTA (Coffea canephora P.) DALAM SEDIAAN GEL DAN CAIR Rahmat Hidayat; Nining Sugihartini; Hari Susanti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.762

Abstract

Sediaan pembersih wajah (face wash) berfungsi untuk membersihkan sekaligus mencegah timbulnya masalah kulit wajah. Penambahan bahan aktif berupa ekstrak daun kelor dan ekstrak biji kopi diharapkan akan meningkatkan manfaat sediaan face wash tersebut. Tujuan dari penelitian ini untuk mengetahui jenis sediaan yang paling optimal antara sediaan gel dan cair serta variasi komposisi yang optimal antara kedua ekstrak dalam formulasi face wash. Face wash diformulasikan dalam bentuk sediaan gel dan cair dengan variasi komposisi ekstrak daun kelor (Moringa oleifera L.) dan ekstrak biji  kopi robusta (Coffea canephora P.) secara berturut-turut yaitu 1:1, 1:2, 2:1. Sediaan dievaluasi berdasarkan parameter organoleptis, pH, viskositas, tinggi busa dan stabilitas busa serta daya hambat terhadap bakteri Staphylococcus aureus. Data yang diperoleh kemudian di analisa Two Way Anova dalam aplikasi SPSS versi 23. Berdasarkan uji diketahui bahwa face wash gel lebih baik pada uji organoleptis, pH, tinggi busa dan stabilitas busa, viskositas dan daya hambat terhadap bakteri. Peningkatan komposisi ekstrak biji kopi robusta menyebabkan peningkatan pH pada jenis sediaan face wash gel maupun cair. Berdasarkan data maka dapat disimpulkan jenis sediaan face wash gel pada varian komposisi zat aktif ekstrak biji kopi robusta 10% dan ekstrak daun kelor 20% lebih baik dan paling sesuai standar yang ditetapkan SNI (Standar Nasional Indonesia). Kata kunci : Face wash, Ekstrak daun kelor, Ekstrak kopi robusta, gel
Pemanfaatan Ampas Kopi sebagai Sediaan Body Scrub di SMK Muhammadiyah 3 Yogyakarta Santoso, Blegoh Iwan; Rohmah, Siti; Susanti, Hari
Jurnal Inovasi Pengabdian dan Pemberdayaan Masyarakat Vol 4 No 1 (2024): JIPPM - Juni 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jippm.408

Abstract

Dalam menghadapi era modern yang berkembang pesat saat ini khususnya dalam pemanfaatan kopi, maka diperlukan suatu inovasi baru yang bertujuan meningkatkan nilai ekonomis dan keterampilan pada siswa smk muhamadiyah 3 yogyakarta. Selama ini, pemanfaatan kopi hanya sebagai produk minuman sehingga harus memiliki inovasi untuk mampu bersaing. Salah satu usaha untuk meningkatkan nilai tambah produk kopi adalah memanfaatkan ampas kopi hasil seduhan untuk dijadikan produk body scrub. Kopi banyak mengandung antioksidan sehingga baik untuk Kesehatan kulit, begitu juga dengan sediaan body scrub yang mampu membantu menghaluskan dan mengangkat sel kulit mati pada tubuh. Metode yang digunakan pada pelaksanaan kegiata pengabdian masyarakat adalah metode survei lapangan, pelatihan, praktek, diskusi, tanya jawab dengan sasaran siswa smk muhamadiyah 3 yogyakarta khusunya kelas XI Farmasi. Dengan diadakannya kegiatan pengabdian masyarakat melalui pelatihan dalam membuat sediaan body scrub dari ampas kopi maka dapat menambah wawasan dan keterampilan siswa smk muhamadiyah 3 yogyakarta dalam mengolah produk kopi dalam bidang kosmetik dengan melihat nilai posttest yang lebih baik atau meningkat dari nilai pretest.
STABILITY TEST OF CLOVE FLOWER ESSENTIAL OIL OINTMENT (SYZIGIUM AROMATICUM L) WATER SOLUTION BASED IN PLASTIC AND GLASS PACKAGING Afriyani, Neti; Susanti, Hari; Sugihartini, Nining
Jurnal FARMASIMED (JFM) Vol 5 No 1 (2022): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v5i1.1254

Abstract

Cengkeh (Syzygium aromaticum L) merupakan salah satu tanaman herbal yang sering digunakan masyarakat karena kandungan utama minyak atsiri bunga cengkeh (Syzigium aromaticum) yang utama adalah eugenol yang memiliki khasiat sebagai antiinflamasi. Formulasi salep telah dilakukan dengan membuat sediaan salep dalam basis larut air (PEG). Salah satu persyaratan sediaan salep adalah stabil. Penggunaannya secara tradisional memerlukan waktu penyiapan yang lama sehingga perlu formulasi sediaan yang lebih praktis dan stabil dalam penyimpanan. Tujuan penelitian ini untuk mengetahui stabilitas salep minyak atsiri bunga cengkeh (Syzigium aromaticum) dalam basis larut air (PEG) yang baik dan optimal. Minyak atsiri bunga cengkeh diformulasi dalam bentuk sediaan salep basis larut air dalam kemasan tube plastik dan gelas. Evaluasi dilakukan pada uji stabilitas sifat fisika meliputi organoleptik, viskositas, pH, sentrifuga, freezer thaw Uji stabilitas dilakukan penyimpanan pada Climatic chamber dengan kondisi suhu 40°C ± 2°C kelembaban 75% RH ± 5% selama 90 hari kemudian diuji pada hari ke-0, ke-7, ke-14, ke-30, ke-60 dan hari ke-90. Analisis data secara statistik menggunakan SPSS metode two way ANNOVA. Hasil penelitian menunjukkan bahwa pengemas plastik dapat menurunkan viskositas dan pH. Kombinasi polietilen glikol (PEG) 400 dan polietilen glikol (PEG) 4000 dapat membentuk formula optimum dengan perbandingan 67% polietilen glikol (PEG) 400 dan 28% polietilen glikol (PEG) 4000 dalam rancangan formula.