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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Jurnal Medik Veteriner ARSHI Veterinary Letters Jurnal Kedokteran Hewan Asian Journal of Social and Humanities

Anita Esfandiari

IPB University

Author-ID : 2444361

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Veterinary

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Profil Imunoglobulin-G Serum Kambing Peranakan Etawah Bunting yang Diberi Imbuhan Pakan Mineral Seng (SERUM IMUNOGLOBULIN-G LEVEL ON PREGNANT ETTAWAH CROSSBRED WERE GIVEN ZINC MINERAL) Sus Derthi Widhyari; Anita Esfandiari; I Ketut Sutama; Setyo Widodo; I Wayan Teguh Wibawan; Rizal Rahadian Ramdhany
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this study was to find out the effect of zinc supplementation on the profile of immunoglobulin-G (IgG) in the pregnant etawah-cross ewes. Fifteen etawah-cross ewes (3-6 years old and 30-50 kg body weight) were used in this experiment and they were divided into three groups each of which consisted of 5 ewes. The first group (Zn40) received 40 ppm Zn as control, the second group (Zn60) received 60 ppm Zn, and the third group (Zn80) received 80 ppm Zn. Blood samples were collected from jugular vein every two weeks, started at twelve weeks of pregnancy up to eight weeks post partum for immunoglobulin-G analysis. IgG level were analyzed by competitive Enzyme Linked Immunosorbent Assay (ELISA) technique. Results showed that there were no significant difference of immunoglobulin-G level (P>0,05) among the three treatment groups ( Zn40, Zn60, and Zn80). However, IgG level of Zn40 and Zn80 groups tended to decrease during the last staget of pregnancy until two weeks post partum. In conclusion, supplementation of 60 ppm Zn showed higher IgG level than Zn 80 ppm. ABSTRAK Penelitian ini bertujuan untuk mengetahui efek suplementasi seng (Zn) terhadap imunoglobulin-G (IgG) pada kambing peranakan etawah (PE) bunting. Penelitian ini menggunakan 15 ekor kambing PE umur sekitar tiga sampai enam tahun, bobot badan sekitar 30-50 kg dibagi ke dalam tiga kelompok, masing-masing terdiri dari lima ekor. Kelompok Zn40 (kontrol) diberi pakan mengandung mineral Zn 40 ppm, kelompok Zn60 mengandung mineral Zn 60 ppm, dan kelompok Zn80 mengandung mineral Zn 80 ppm. Sampel darah diambil melalui vena jugularis untuk menganalisis konsentrasi IgG. Pengambilan sampel darah dilakukan setiap dua minggu mulai umur kebuntingan 12 minggu sampai delapan minggu setelah melahirkan. Hasil penelitian menunjukkan bahwa konsentrasi IgG serum tidak menunjukkan perbedaan secara nyata antar kelompok (P>0,05) antara kelompok Zn 0, Zn 40 dan Zn 80 ppm. Kelompok Zn 40 ppm dan 80 ppm cendrung memperlihatkan konsentrasi IgG mengalami penurunan pada akhir kebuntingan sampai dua minggu setelah melahirkan. Pada kelompok Zn 60 ppm memperlihatkan konsentrasi IgG lebih tinggi dibanding kelompok Zn 80 ppm.

Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Anita Esfandiari; Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; Dondin Sajuthi; I Ketut Sutama
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.331 KB)

This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.

Performans Produksi dan Profil Metabolik Darah Domba Garut dan Jonggol yang Diberi Limbah Tauge dan Omega-3 (PRODUCTION PERFORMANCE AND BLOOD METABOLIC PROFILES OF GARUT AND JONGGOL RAMS THAT WAS FED MUNG BEAN SPROUT WASTE AND OMEGA-3) Gagah Hendra Wijaya; Mohamad Yamin; Henny Nuraini; Anita Esfandiari
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.722 KB)

The research objectives were to evaluate and compare of production performances and blood metabolicprofiles of garut and jonggol rams fed with mung bean sprout waste and omega-3. This research used 24rams aged of 1-2 years, consisted of 12 garut rams weighed of 36,43±1,45 kg (CV=13,87%) and 12 jonggolrams weighed of 23,09±0,57kg (CV=8,64%). Research used Completely Randomized factorial Design(CRD 2x3) with 2 main treatments x 3 factorials and four replications. The first factors as the maintreatments were different breeds of garut and jonggol. The second factors as the factorial treatments werethree different feed percentages which consisted of P0 (40% grass+60% concentrate I), P1 (40% mung beansprouts waste+60% concentrate II), and P2 (40% mung bean sprouts waste+60% concentrate II+omega-3).Data were analyzed by analysis of variance (ANOVA) and proceeded by Duncan Range Test. The resultsshowed that P1 had the highest performance on feed efficiency and average daily gain (ADG) parameters.Blood metabolic test showed that blood total protein, cholesterol, and glucose of all rams were normal. Inconclusion feeding rams with mung bean sprout waste as grass substitution have a positive effect on theperformance of the rams production performance.

Profil Elektrolit Serum Pedet Sapi yang Diinfeksi Escherichia coli K-99 dan Diberi Mikrokapsul Imunoglobulin-G Anti E. coli Arief Purwo Mihardi; Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Escherichia coli (E. coli) K-99 infection cause acute diarrhea in calves which may reduce electrolyte, such as sodium and potassium. The experiment was conducted to study the sodium and potassium profiles in calves infected by E. coli and received immunoglobulin G anti E. coli K99 microcapsules. Sixteen calves, 4 – 6 days years old, were grouped into four groups, consists of four calves, i.e. negative control/NC group (the calves were not infected by E. coli K-99 and no treatments); positive control/PC group (the calves were infected by E. coli K-99 and no treatments); treatment 1/P1 group (the calves were infected by E. coli K-99 and received colostrum suspension of IgG anti E. coli K-99); and treatment 2/P2 group (the calves were infected by E. coli K-99 and received microcapsules of IgG anti E. coli K-99). Escherichia coli K-99 were infected to all calves except the NC group orally with the dosage of 5 × 1010 cfu/ml. Suspension and microcapsules of IgG anti E. coli K-99 for each calf were given orally with dosage of 1.76 gram IgG anti E. coli K-99 per day. Serum were collected from jugular vein at 0, 24, 48, 72, and 168 hours after E. coli K99 infection. The sodium and potassium concentrastion were analyzed using a spectrophotometer. Results of the experiment indicated that the concentration of sodium and potassium were not significantly different among groups. Sodium concentration of P2 group was relatively higher than PC or P1 and showed increased concentration 168 hours after infection. Potassium concentration of P2 were higher than the PC group, but lower than NC and P1 group. In conclusion, the administration of IgG anti E. coli K-99 microcapsules was not significantly change the sodium and potassium concentration, but may maintain the stability of sodium level in calves infected by E. coli K-99.

Studi Histologi Tubulus Ginjal Tikus Model Hipertensi yang Diterapi Menggunakan Bone Marrow Mesenchymal Stem Cell Tiara Widyaputri; Erni Sulistiawati; Dondin Sajuthi; Anita Esfandiari; Setyo Widi Nugroho
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Akhir-akhir ini banyak peneliti yang mempelajari terapi berbasis sel sebagai terapi regeneratif untuk melindungi atau memperbaiki jaringan yang rusak. Bone marrow mesenchymal stem cell (BMMSC) memiliki kemampuan yang menjanjikan dalam memperbaiki ginjal. Tujuan penelitian ini adalah untuk mengetahui apakah induksi yang dilakukan dapat menyebabkan kerusakan tubulus ginjal dan BMMSC mampu melindungi atau memperbaiki kerusakan yang terjadi. Penelitian ini menggunakan dua belas tikus jantan umur 10-12 minggu dengan rata-rata tekanan darah sistolik minimum mencapai 140-150 mmHg yang dibagi menjadi kelompok kontrol dan kelompok terapi. Tekanan darah yang tinggi diinduksi dengan mengangkat ginjal kanan, mengikat arteri karotis komunis kiri, serta memberikan NaCl 1%, DOCA 2,5 mg/100gBB, dan BAPN 0,12%. Bone marrow mesenchymal stem cell disuntikkan setelah 16 minggu induksi. Evaluasi histopatologi ginjal yang didukung dengan evaluasi darah dilakukan dua minggu setelah injeksi BMMSC. Berdasarkan evaluasi histopatologi, kerusakan tubulus ginjal pada dua kelompok tidak menunjukkan hasil yang berbeda. Demikian juga regenerasi pada kedua kelompok menunjukkan hasil sama. Kadar blood urea nitrogen (BUN) dan kreatinin pada kedua kelompok berada dalam kisaran kadar normal. Hasil yang didapat menunjukkan bahwa kerusakan yang terjadi tidak parah dan BMMSC memperbaiki tubulus ginjal akan tetapi belum dapat melindungi tubulus ginjal dari kerusakan.

Dinamika Total Protein Serum Tikus Putih (Rattus novergicus) yang Diberi Mikrokapsul Imunoglobulin-G Anti-H5N1 (DYNAMICS OF TOTAL PROTEIN SERUM OF RATTUS NOVERGICUS GIVEN MICROCAPSULES OF IMMUNOGLOBULIN G ANTI H5N1) Kathirina Beatrik Riwu Wolo; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Immunoglobulin G (IgG) from colostrum of hyperimmune colostrum obtained from vaccinated cattle with H5N1 vaccine is potential to be developed into passive immunotherapy product for “bird flu” infection. The administration of passive therapy can be done orally, but the environmental conditions of the digestive tract can cause IgG damage, so the microencapsulation technique was used in this study to protect IgG. This study aimed to evaluate the absobrption of H5N1 IgG anti-Avian Influenza which was microencapsulated using rats (Rattus novergicus) as animals model. This study was conducted using 108 adult male white rats (Rattus norvegicus), Wistar strain, approx. 250 grams of body weight. Rats were divided into three treatment groups (N = 36), i.e. control group (K) were given aqua distilled water, suspension group (S) were given anti-H5N1 IgG suspension, and microcapsules group (M) were given microcapsules equivalent to suspension with the titer of 25. Necropsy was carried out at four observation points (2, 4, 6 and 8 hours) to collected blood through the heart and collected in vacutainer without anticoagulants to obtain serum. The blood serum then analyzed for total protein and globulin and albumin/globulin ratio (A/ G). The results showed that the level of total protein and globulin group M was higher and significantly different (P> 0.05) compared to K and S at the end point of observation. The A/G ratio in group K showed a significant increase (P> 0.05) at the end point of observation while in group M there was a marked decrease (P> 0.05) at the time point of 2 hours to 4 hours and then constant until the end of observation. These results showed that administration of anti-H5N1 IgG microcapsules can increase total levels of protein and globulin in the blood.

Profil Kadar Kortisol dan Seng pada Kambing Peranakan Etawah Saat Melahirkan yang Diberi Tambahan Seng dalam Pakannya Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; I Ketut Sutama; Anita Esfandiari
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.536 KB)

The objective at this experiment was to study the role of zinc supplementation (Zn) in minimizingstress at parturition. Thirty etawah cross breed age 3-6 years with average body weight 30-50 kg were usedin this study and divided into three groups ; i) animal were given grass + feed concentrate + ZnSO4 40 mg/kg dry matter ( control group) ; (ii) grass + feed concentrate + ZnSO4 60 mg/kg dry matter (treatment 1), and(iii) grass + feed concentrate + ZnSO4 80 mg/kg dry matter (treatment 2), respectively drinking water weregiven ad libitum. To monitor zinc and cortisone level, blood samples were withdrawn from each animalevery two weeks starting at age of pregnancy 3 months up to two months post parturition, in addition, tomonitor the cortisone level blood samples were also collected at 1,2,3 and 7 days post parturition. The Znand cortisone serum were analyzed using atomic absorbent spectro photometric (AAS) andradioimmunoassay (RIA), respectively the result showed that generally pregnancy at the beginning andget serum level at cortisone was relatively similar between the three animal groups and there was atendency to increase and reached peak at parts then gradually decrease until the and of the observation. Atparturition the lowest serum level at cortisone (19.00±18.72 mg/ml) was observed in animals receiving the60/ dry matter zinc supplement compared to animal receiving 80 mg/ dry matter zinc ( 52.65 ± 30.83 mg/ml) and control animal ( 75.92 ± 42.88 mg/ml). in addition serum level at Zn was significantly higher inanimal receiving 60 mg and 80 mg Zn in their diets compared to control animals. The best profiles wereseen in animal given 60 mg/kg dry matter Zn supplement. In conclusion, the addition of Zn in the diets hadsignificant effect in minimizing stress as pictured by the low serum level at cortisone at parturition.

Profil Leukosit Serta Imbangan Neutrofil dan Limfosit pada Kambing Peranakan Etawah yang Sedang Bunting Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; Anita Esfandiari; Chusnul Choliq
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This research aimed to determine the profiles of total leukocytes and neutrophil -lymphocyte ratio (N/L) in pregnant etawah crossbreed goats. Six etawah crossbreed goats around 3-6 years old and body weight around 30-50 kg were used in this study. Blood samples were taken from jugulars vein in non pregnant (K), and at gestational age 12, 14, 16 and 18 weeks. Blood is inserted into a tube that containsEDTA (Ethylene Diamine Tetraacetic Acid) anticoagulant to obtain whole blood. Whole blood were analyzed to total leukocyte cells and leukocyte types. The results showed that total leukocytes profiles tend to increase during pregnancy. Lymphocytes stable until 16 weeks of pregnancy and tends to decrease at the end of pregnancy. The N/L ratio increase at the end of pregnancy, this condition indicates an increase in the number of neutrophils accompanied by decreased lymphocyte cells and can be found in etawah crossbreedat 18 weeks of gestation.

KONDISI NEONATUS ANAK BABI YANG DILAHIRKAN OLEH INDUK YANG DISUNTIK GONADOTROPIN SEBELUM PENGAWINAN Friska Mery Montolalu; Anita Esfandiari; Damiana Rita Ekastuti; Wasmen Manalu
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Injections of sows with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) prior to mating have been shown to improve endogenous secretions of pregnant hormones that improve and optimize the uterine environment and condition during pregnancy that eventually lead to give birth to superior piglets. This experiment was designed to study the effects of PMSG and hCG injections of sows prior to mating on prenatal growth as indicated by the conditions of the the neonate piglets. Thirty mature female Landrace pigs were divided into 2 groups i.e., 15 pigs were injected with PMSG and hCG prior to mating, and the other 15 pigs were injected with physiological NaCl solution as a control. Before mating, the estrus cycles of the experimental sows were synchronized by injecting prostaglandin two times with 14 days interval. The experimental pigs were injected with PMSG and hCG or 0.90% NaCl solution at the same time with the second prostaglandin injection and were further mated naturally. During pregnancy, the experimental sows were maintained with the normal management condition. The results showed that piglest born to sows injected with PMSG and hCG prior to mating had shorter and faster birth process with higher activities and survivals as compared to those born to control sows. Piglets born by sow that were injected with PMSG and hCG before mating had a more optimum birth weight accompanied by greater body length, limb height, and higher rear limb height. Piglest born to by sows that were injected with PMSG and hCG prior to mating showed superior conditions that would contribute to optimum and superior preweaning and postweaning growths until maturity.

Keberhasilan Hiperimunisasi Pada Sapi Bunting: Kajian Terhadap Ketepatan Waktu Vaksinasi Safitria Wulandari; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk melihat pengaruh umur kebuntingan induk sapi saat vaksin pertama kali diberikan terhadap keberhasilan hiperimunisasi. Sebanyak 12 ekor induk sapi bunting jenis sapi Friesian Holstein, dibagi menjadi kelompok kontrol dan vaksin, dengan masing-masing kelompok terdiri dari umur kebuntingan enam bulan (n=3) dan tujuh bulan (n=3). Induk sapi diinjeksi vaksin Avian influenza (AI) H5N1 komersial (killed vaccine) sub-kutan, sebanyak 5 dosis/ekor (106 EID50/dosis). Vaksinasi dilakukan dua kali dengan interval antar vaksinasi selama dua minggu. Sebelum vaksinasi, induk sapi diberi imunomodulator 0,1 mg/kg bb per oral selama tiga hari berturut-turut kemudian dilakukan priming dengan menyuntikkan antigen AI H5N1 inaktif tanpa adjuvant (intravena) tiga hari berturut-turut (10x28 HAU/ekor). Sampel darah dikoleksi melalui vena coccygea sebelum vaksinasi pertama, dua minggu pascavaksinasi I dan dua minggu pascavaksinasi II untuk dianalisis terhadap parameter leukosit (jumlah total leukosit dan limfosit), kadar total protein, globulin dan rasio albumin/ globulin (A/G), serta titer IgG anti AI H5N1 dalam darah dan kolostrum. Hasil pengamatan menunjukkan tidak terdapat perbedaan yang signifikan pada jumlah total leukosit dan limfosit, kadar total protein dan globulin, rasio A/G, serta titer IgG anti AI H5N1 dalam darah dan kolostrum, baik antar kelompok maupun antar waktu pengamatan (p>0.05). Namun demikian, pada induk sapi kelompok vaksin umur kebuntingan enam bulan menunjukkan parameter leukosit, kadar total protein dan globulin serta titer IgG anti AI H5N1 dalam darah dan kolostrum lebih tinggi dibandingkan kelompok induk sapi umur kebuntingan tujuh bulan. Umur kebuntingan pada saat vaksinasi pertama kali dilakukan tidak memengaruhi keberhasilan hiperimunisasi pada induk sapi bunting trimester akhir.

Co-Authors . Herlina . Suryono Aditia Dwi Cahyono Agus Lelana Agus Setiyono Agus Wijaya Agus Wijaya Ali Hujarat Amrozi Arief Purwo Mihardi Arif Purwo Mihardi Arifianto, Dinar Azzahrah, Putri Bambang Pontjo Priosoeryanto Bayu Febram Bayu Firmala Kusuma Chusnul Choliq Chusnul Choliq Damiana Rita Ekastuti Darsono Darsono Deni Noviana Deny Setyo Wibowo Dewi, Fitriya Nur Annisa Dondin Sajuthi Dwi Utari Rahmiati Effendi, Siti Aisyah Rahmalia Erni Sulistiawati Fitri, Arni Diana Friska Mery Montolalu Gagah Hendra Wijaya Gunanti Gunanti . Henny Nuraini Huda Shalahudin Darusman I Ketut Sutama I wayan Teguh Wibawan Ida Zahidah Irfan IETJE WIENTARSIH Iman, Bintang Nurul Joan Elviyanti Joesoef, Jayusman Arsiyanti Kartjito, Nicolas Edward Christanto Kathirina Beatrik Riwu Wolo Khairita, Lia Kusumawati, Nina Tri Leni Maylina Lia Khairita Lina Noviyanti Sutardi Lingga Surya Maret Daulay Madyastuti, Rini Maharani Maharani, Maharani Maret Daulay, Lingga Surya Mohamad Yamin Nina Kusumawati Ningtias, Putri Indah Nuzul Asmilia Nuzul Asmilia Retno Wulansari Rizal Arifin Akbari Rizal Rahadian Ramdhany Robby Wienanto Robby Wienanto Safitria Wulandari Setiadi, Hirawan Setyo Widi Nugroho Setyo Widodo Setyo Widodo Soesatyoratih, Rr Solinda, Gamelita Rizkawandi sri murtini . Sri Wahyuni Subangkit, Mawar Suryo Saputro Sus Derthi Widhyai Sus Derthi Widhyari Sus Derthi Widhyarti Tiara Widyaputri Virgilius Martin Kelake Kedang Virgilius Martin Kelake Kedang Wasmen Manalu Wiwin Winarsih Wolo, Kathirina Beathrik Riwu

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