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Cultivating Microalgae Botryococcus braunii in Tofu Whey Medium Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 1 (2022): April 2022
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.507 KB) | DOI: 10.14710/jbes.2022.14234

Abstract

Tofu waste water is still being a significant issue in Indonesia owing to its level of BOD (Biological Oxygen Demand) and COD (Chemical Oxygen Demand) (Chemical Oxygen Demand). However, this waste also includes significant ammonia (230 mg/L) which is needed for microalgae development. Microalgae are photosynthetic microorganism which need nitrogen supply for their development. Among others, microalgae Botryococcus braunii is the one with large oil amount within their cells (25 – 75 %). This study was meant to examine the usage of tofu whey for culture medium for algae development. The experiment was done by adjustment of whey volume (5-20 %) in fresh medium and algal biomass was continually measured. The findings revealed that Botryococcus braunii obtained optimum growth in 10 % volume with biomass generated at 2.4 g/L and 0,8716 g/L of lipid production. At this circumstance, COD might be lowered up to 83.33 %.
Palm Oil Milling Effluent (POME) Waste Processing by Using Microalgae Chlamydomonas sp. Riky Yonas; Uray Irzandi; Hantoro Satriadi; W. Widayat; Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.15152

Abstract

Along with the growth in oil palm output, the amount of trash produced will also increase. Every palm oil mill is responsible for the disposal of liquid waste known as palm oil mill effluent (POME). POME includes very high levels of BOD and COD, which may hinder the development of microalgae. Before POME may be utilized as a medium for the growth and development of microalgae, a detailed investigation is required to establish the pretreatment measures necessary to reduce the BOD and COD levels. The purpose of this investigation of POME waste as a substrate for the growth and development of microalgae is to examine the POME processing procedure utilizing wild microalgae. The experimental technique consisted of adding POME and microalgae to the Erlemeyer in accordance with the required proportion. Research demonstrates that POME pond IV waste may be utilized as a substrate for the development of wild microalgae to lower POME waste BOD and COD levels. The variables used were the ratio of POME to microalgae volume and the quantity of nutrients supplied. Microalgae growth at a ratio of 1:4 produced the greatest decreases in BOD and COD, namely 61.66 ppm and 173.33 ppm from 110.6 ppm and 496.67 ppm, respectively. The impact of adding nutrient C at a concentration of 120 ppm led to the greatest decrease of BOD and COD, namely 65.33 ppm and 186.67 ppm, whereas adding nutrient N at a concentration of 40 ppm led to the greatest reduction of BOD and COD, namely 55.41 ppm and 158.33 ppm.
Effect of Storage on The Chemical Quality of Pasteurized Milk with Supplemented Soybean Oil and Phycocyanin Angela Nitia Nefasa; Ega Zahrotun Nisa; Marcelinus Christwardana
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14728

Abstract

The interaction between the addition of soybean oil and phycocyanin extract on the protein, fat, and solid non-fat content of pasteurized milk after a one-week storage period is highly important to investigate. Soybean oil is used to improve the quality of pasteurized milk, particularly its chemical quality, consisting of fat, protein, and Solid Non-Fat (SNF). Phycocyanin extract provides a source of protein, whereas soybean oil contains healthy fatty acids. Before the fresh milk is pasteurized, soybean oil and phycocyanin are added. The LTLT (Low Temperature Long Time) technique was used to pasteurize the milk at 63 °C for 30 minutes. After the pasteurization procedure was completed, the milk samples were refrigerated for one week at a temperature of 4 °C. Following the storage period, the chemical composition of pasteurized milk is examined. With the addition of soybean oil and phycocyanin extract, the protein content of milk rose, as shown by the findings. The sample T1 with a concentration of 0.45% soybean oil and 0.50% phycocyanin extract had the greatest amount of protein (3.58). The sample T2 with 0.45% soybean oil concentration and phycocyanin extract (1%) had the greatest fat content (6.4%). Adding soybean oil and phycocyanin extract enhanced the total SNF concentration. On the basis of the study conducted, it can be concluded that the addition of soybean oil and phycocyanin extract to pasteurized milk has an influence and interaction on the milk's protein, fat, and SNF content. The addition of soybean oil and phycocyanin extract to pasteurized milk held for one week may boost protein, fat, and SNF concentrations.
Cultivation of Microalgae Spirulina platensis in Palm Oil Mill Effluent (POME) Media with Variations of POME Concentration and Nutrient Composition Fitria Yuli Anggita Sari; I Made Aditya Suryajaya; Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.15052

Abstract

Indonesia and Malaysia are recognized as the world's leading producers of palm oil. Along with the growth of the palm oil industry in Indonesia, the amount of Palm Oil Mill Effluent has increased. Palm Oil Mill Effluent (POME) is a liquid byproduct of the palm oil production process. POME has been treated using aerobic and anaerobic ponds to lower Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD) levels, but it still includes nutrients like as C,N,P that are beneficial to the development of microalgae. On this study, Spirulina platensis was grown in POME medium with 20%, 40%, and 60% V. concentrations. Every two days, the nutrients Urea, NaHCO3, and TSP were administered. Seven days of aeration and 24-hour lights are used throughout the cultivation phase. The results indicate that POME with a 20 % concentration is the optimal medium for plant growth. Add 25 ppm Urea, 50 ppm TSP, and 200 ppm NaHCO3 for the optimal nutritional composition. At the same treatment on various medium, the highest growth rate of Spirulina platensis is determined to be µ = 0.128% per day, with an optical density of 0.648. Carbon reductions range from 83.03 % to 84.10 %, while Nitrogen savings range from 78 % to 79.55 % when POME is used as a growing medium. This study also shown that the C, N, and P concentrations of POME fall by 93 to 98 %, 99 to 99.5 %, and 92 to 97 %, respectively.
Evaluation of Drying Air Conditions for Antiozonant Wax Drying Process in the Spray Drying Tower Marcelinus Christwardana; Ifa Miftahushudury
ASEAN Journal of Chemical Engineering Vol 21, No 2 (2021)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ajche.64352

Abstract

The drying technique of Antiozonant Wax (AOW) using drying air in the spray drying tower has a considerable effect on the produced AOW powder. In this study, the drying air flow rate was measured in such a way that AOW can transform into a powder with a size of 800 mesh. The diameter and height of the spray drying tower are 1 and 6 m, respectively. Meanwhile, the AOW flow rate to the spray drying tower varies from 100 kg/hour to 500 kg/hour. The intake AOW temperature was 70 °C and at the outlet was 40 °C, while the drying air temperatures in and out of the spray drying tower were 30 and 55 °C, respectively. From the calculation results, the flow rate of the drying air is directly proportional to the flow rate of the AOW into the spray drying tower but inversely proportional to the speed of the AOW powder down the spray drying tower. In the meantime, the drying period for AOW to become a powder is between 1.033 – 1.279 s, not significantly different. It gives insight into the need to dry air in the spray drying tower configuration so that the findings will conform to the predetermined requirements.
Zinc Removal from ZnO Industrial Wastewater by Hydroxide Precipitation and Coagulation Methods: The Role of pH and Coagulant Dose Ratnawati Ratnawati; Marcelinus Christwardana; Sudirman Sudirma; Enjarlis Enjarlis
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 11 No. 2 (2020)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2020.v11.no2.p35-42

Abstract

Liquid waste from the ZnO industry must be treated to meet the quality standards of wastewater into water bodies, according to the Minister of Environment Regulations No.5, 2014. It still contains 79 mg/L of Zn metal, cloudy with turbidity above 500 NTU, and COD value around 222 mg/L. This study aims to determine the effect of pH on reducing Zn metal and the coagulant dose to minimize turbidity and COD in liquid waste produced by the ZnO factory in Depok, West Java. The waste treatment has been carried out by adding alkaline to neutralize the acid conditions in the equalization basin. However, the results have not met the requirements. It is necessary to vary the pH (8.5; 9.0; 9.5; 10.0 and 10.5) to precipitate of Zn optimally, modify the dose of coagulants (50; 100 and 150 mg/L) and reaction times (10; 15 and 20 minutes) to reduce its turbidity and COD concentration. The best results were obtained at a pH of 9.5 with a coagulant dose of 50 mg/L and a reaction time of 10 minutes. This condition can reduce Zn concentration (79 to 3.71 mg/L), turbidity (557 to 1.42 NTU), COD (222 to 68 mg/L) with a removal efficiency of 95.3%; 99.7%; and 69.4% respectively. These values have met the standard requirements according to government regulations.
Cultivating Microalgae Botryococcus braunii in Tofu Whey Medium Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 1 (2022): April 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14234

Abstract

Tofu waste water is still being a significant issue in Indonesia owing to its level of BOD (Biological Oxygen Demand) and COD (Chemical Oxygen Demand) (Chemical Oxygen Demand). However, this waste also includes significant ammonia (230 mg/L) which is needed for microalgae development. Microalgae are photosynthetic microorganism which need nitrogen supply for their development. Among others, microalgae Botryococcus braunii is the one with large oil amount within their cells (25 – 75 %). This study was meant to examine the usage of tofu whey for culture medium for algae development. The experiment was done by adjustment of whey volume (5-20 %) in fresh medium and algal biomass was continually measured. The findings revealed that Botryococcus braunii obtained optimum growth in 10 % volume with biomass generated at 2.4 g/L and 0,8716 g/L of lipid production. At this circumstance, COD might be lowered up to 83.33 %.
Palm Oil Milling Effluent (POME) Waste Processing by Using Microalgae Chlamydomonas sp. Riky Yonas; Uray Irzandi; Hantoro Satriadi; W. Widayat; Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.15152

Abstract

Along with the growth in oil palm output, the amount of trash produced will also increase. Every palm oil mill is responsible for the disposal of liquid waste known as palm oil mill effluent (POME). POME includes very high levels of BOD and COD, which may hinder the development of microalgae. Before POME may be utilized as a medium for the growth and development of microalgae, a detailed investigation is required to establish the pretreatment measures necessary to reduce the BOD and COD levels. The purpose of this investigation of POME waste as a substrate for the growth and development of microalgae is to examine the POME processing procedure utilizing wild microalgae. The experimental technique consisted of adding POME and microalgae to the Erlemeyer in accordance with the required proportion. Research demonstrates that POME pond IV waste may be utilized as a substrate for the development of wild microalgae to lower POME waste BOD and COD levels. The variables used were the ratio of POME to microalgae volume and the quantity of nutrients supplied. Microalgae growth at a ratio of 1:4 produced the greatest decreases in BOD and COD, namely 61.66 ppm and 173.33 ppm from 110.6 ppm and 496.67 ppm, respectively. The impact of adding nutrient C at a concentration of 120 ppm led to the greatest decrease of BOD and COD, namely 65.33 ppm and 186.67 ppm, whereas adding nutrient N at a concentration of 40 ppm led to the greatest reduction of BOD and COD, namely 55.41 ppm and 158.33 ppm.
A Mini Review on Technique of Milk Thermization Angela Nitia Nefasa; Marcelinus Christwardana; Zakaria Hussein Abdurrahman; Fatkur Rohman; Agus Afif
Journal of Bioresources and Environmental Sciences Vol 2, No 3 (2023): December 2023
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2023.19916

Abstract

Thermization is a preheating procedure in which milk is heated at low temperatures for a brief time prior to further processing. It is often used in the dairy processing industry to make a variety of products, including pasteurized milk, milk powder, and cheese. Eliminating psychrophilic bacteria, avoiding milk spoiling, and halting the changes generated by lipase and protease enzymes are the objectives of this method. Despite the fact that thermization enhances the shelf life of milk, it may also result in sensory changes and the germination of B. cereus spores. The goal of the thermization method is to prepare milk for the upcoming processing stage, and the temperature and length of the process might vary. Thermization does not affect the flavor of milk or dairy products such as yogurt, but it may enhance the quality of cheese by decreasing the occurrence of stale and rancid flavors. The process may be carried out in a plate heat exchanger or as a pre-pasteurization treatment for raw milk to protect milk quality during lengthy storage in insulated silos.
Effect of Storage on The Chemical Quality of Pasteurized Milk with Supplemented Soybean Oil and Phycocyanin Angela Nitia Nefasa; Ega Zahrotun Nisa; Marcelinus Christwardana
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14728

Abstract

The interaction between the addition of soybean oil and phycocyanin extract on the protein, fat, and solid non-fat content of pasteurized milk after a one-week storage period is highly important to investigate. Soybean oil is used to improve the quality of pasteurized milk, particularly its chemical quality, consisting of fat, protein, and Solid Non-Fat (SNF). Phycocyanin extract provides a source of protein, whereas soybean oil contains healthy fatty acids. Before the fresh milk is pasteurized, soybean oil and phycocyanin are added. The LTLT (Low Temperature Long Time) technique was used to pasteurize the milk at 63 °C for 30 minutes. After the pasteurization procedure was completed, the milk samples were refrigerated for one week at a temperature of 4 °C. Following the storage period, the chemical composition of pasteurized milk is examined. With the addition of soybean oil and phycocyanin extract, the protein content of milk rose, as shown by the findings. The sample T1 with a concentration of 0.45% soybean oil and 0.50% phycocyanin extract had the greatest amount of protein (3.58). The sample T2 with 0.45% soybean oil concentration and phycocyanin extract (1%) had the greatest fat content (6.4%). Adding soybean oil and phycocyanin extract enhanced the total SNF concentration. On the basis of the study conducted, it can be concluded that the addition of soybean oil and phycocyanin extract to pasteurized milk has an influence and interaction on the milk's protein, fat, and SNF content. The addition of soybean oil and phycocyanin extract to pasteurized milk held for one week may boost protein, fat, and SNF concentrations.
Co-Authors . Widayat A Abdullah Adrianus Kristyo Prabowo Adrianus Kristyo Prabowo, Adrianus Kristyo Agam Duma Kalista Wibowo Agnes Priska Tiarasukma Agnes Priska Tiarasukma Agus Afif Andin Vita Amalia, Andin Vita Andri Cahyo Kumoro Angela Nitia Nefasa Angela Nitia Nefasa Angela Nitia Nefasa Aniek Sri Handayani Athanasia Amanda Septevani, Athanasia Amanda Berkah Fajar Tamtomo kiono Boy Frando Sijabat Danny Soetrisnanto Deni Shidqi Khaerudini Dessy Ariyanti Dessy Ariyanti Didik Setiyo Widodo E. Enjarlis Ega Zahrotun Nisa Eudia Christina Wulandari Fadhilla, Putri Fania, Adhista Fatkur Rohman Fitra Pradhita Fitria Yuli Anggita Sari Gunawan Gunawan H Hadiyanto H. Hadiyanto H. Hadiyanto H. Hadiyanto H. Hadiyanto H. Hargono Hamid Hamid Hantoro Satriadi Haris, Amnan Hendrawan, Muhammad Andiri Heriyanti, Andhina Putri Heru Cahyana Huda, Muhammad Badrul I Made Aditya Suryajaya Ifa Miftahushudury Ismiyarto Ismitarto Ismojo, I. Joelianingsih . Linda Aliffia Yoshi, Linda Aliffia Linda Suyati Lisa Aditya Margaretha Praba Aulia Maulana, Achmad Yanuar Muhamad Zaini Mahdi Ngadiwiyana M.Si. S.Si. Nur Rokhati Ode Resa Fatlan Pina Tiani Purbowatiningrum Ria Sarjono Putra, Alam Eka Ratnawati Ratnawati Retno Ariadi Lusiana Rifa’atunnisa, Rifa’atunnisa Riky Yonas Rinaryadi, Kemilau Permata Hati Rizkan, Muhammad Rizki Nor Amelia S. Slamet Satrio Kuntolaksono Sidik Marsudi Singgih Hartanto Sri Widodo Agung Suedy Sudirman Sudirma Syachrir, Guntur Tony Suryo Utomo Tri Retnaningsih Soeprobowati Udi Harmoko Udi Harmoko Uray Irzandi Wahyu Zuli Pratiwi Widharyanti, Ika Dyah Yasinta Nikita Titisari Yuli Amalia Husnil Zakaria Hussein Abdurrahman