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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Penyuluhan Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kesehatan Lingkungan indonesia JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan

Hadri Latif

IPB University

Author-ID : 2480267

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Hazard Analysis Critical Control Point (HACCP) sebagai jaminan keamanan produk Sarang Burung Walet Tujuan Ekspor ke Tiongkok Rizal Eko Kurniawan; Chaerul Basri; Hadri Latif
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.72-81

ABSTRACT Edible Bird Nest (EBN) is a food product of animal origin that obtains many benefits for Indonesia's export commodities. EBN contains many nutrients in which it is widely utilized in the health sector. EBN products have been exported to various countries and one of them is China. EBN products exported to China have potential harms such as physical, biological, and chemical hazards that pose risks to human health. Therefore, every product of animal origin needs food safety assurance. Hazard Analysis Critical Control Point (HACCP) is a food safety system developed to identify, evaluate, and control food safety hazards. HACCP is a system developed to prevent or reduce hazards to an acceptable extent during the globally adopted production. Through the implementation of a food safety assurance system in the EBN, it is expected to lower the risk of food hazards. This paper discussed HACCP in ensuring food safety of animal origin, especially Edible Bird Nest to fulfill the export requirements of Edible Bird Nest to China. Keywords: Animal-origin Food Safety, HACCP, Edible Bird Nest

Intervensi Berbasis Partisipasi Masyarakat untuk Peningkatan Pengetahuan dan Sikap Kader Kesehatan dalam Pengendalian Rabies di Kabupaten Sukabumi Fitri Hidayati; Etih Sudarnika; Hadri Latif
Jurnal Penyuluhan Vol. 15 No. 1 (2019): Jurnal Penyuluhan
Publisher : Department of Communication and Community Development Sciences and PAPPI (Perhimpunan Ahli Penyuluh Pertanian Indonesia)

Penelitian ini bertujuan untuk mengukur tingkat pengetahuan dan sikap kader posyandu sebelum dan setelah intervensi. Penelitian ini juga membandingkan perbedaan metode ceramah dan buzz grup dalam meningkatkan pengetahuan dan sikap kader. Penelitian ini menggunakan rancangan quasi eksperimen dengan pre-test and post-test design. Sampel terdiri dari 87 kader posyandu dari 4 desa terpilih di Kecamatan Jampang Tengah. Penelitian dilaksanakan dengan mengintervensi 43 kader menggunakan metode ceramah dan mengintervensi 44 kader menggunakan metode buzz. Kedua metode tersebut dilengkapileaflet, poster dan banneruntuk setiap desa. Data dianalisis dengan menggunakan uji sign dan U Mann Whitney. Penelitian ini menunjukkan bahwa ada perbedaan yang nyata antara tingkat pengetahuan dan sikap kader posyandu sebelum dan sesudah intervensi menggunakan metode ceramah (p=0,000 pengetahuan, p=0,000 sikap). Hasil intervensi menggunakan metode buzz menunjukkan perbedaan yang nyata pada tingkat pengetahuan (p=0,003), tetapi tidak ada perbedaan yang nyata pada tingkat sikap (p=0,096). Hasil penelitian ini juga menunjukkan bahwa metode ceramah dan buzz tidak berbeda nyata dalam meningkatkan pengetahuan dan sikap kader posyandu (P>0,05). Penelitian ini membuktikan bahwa KASIRA mampu meningkatkan pengetahuan kader posyandu.Kata Kunci: Intervensi, kader posyandu, metode ceramah dan buzz, pengetahuan dan sikap, rabies

AQ-9 Identification of Sumateran Wild Boar Meat (Sus scrofa vittatus) by Restriction Fragment Length Polymorphism (RFLP) Analysis of Cytochrome b Gene Melani Wahyu Adiningsih; Retno Damayanti Soejoedono; Trioso Purnawarman; Hadri Latif; Rahmat Setya Adji; Okti Nadia Poetri; Dwi Desmiyeni Putri
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.42 KB)

Sumateran wild boars have been super abundant in Sumateran forest. In Indonesia, this wildlife condition has led to the exploitation for commercial purpose. The high number of Sumateran wild boars population increases wild boar hunting resulting in an abundant availability of wild boar meat in the food market with extremely cheap price. The macroscopic similarity of wild boar meat and beef has prompted the local people to abuse this situation by selling wild boar meat in traditional market as beef. Based on annual record from Cilegon Class II Quarantine Office in 2014, there were nine smuggling cases or a total of 21.556 kg of wild boar meat smuggling effort that were prevented by Cilegon Quarantine officers. The number of food safety concerns related to smuggling of wild boar or counterfeiting beef with wild boar is a very detrimental condition for consumers, especially consumers in traditional markets.The checking of genuineness or validity of food products is an important effort to protect people from consuming unhealthy food and to indicate whether the food is halal or not. Studies of meat detection should be continuously developed as an effort to protect consumers. Genetic method is the most specific and sensitive method to check food ingredients authenticity by detecting the presence of genetic material or deoxyribonucleic acid (DNA). It results from the specific character of the structure of DNA particles and the possibility of using the information included in them. The most frequent loci used for species identificationin phylogenetics and biodiversity studies are mitochondrial cytochrome b (cyt b).Genetic method is the most specific and sensitive tool for analyzing the authenticity of food ingredients in a molecular level by means of detecting the presence of genetic material or deoxyribonucleic acid (DNA). One of the various methods could be used to detect genetic material is polymerase chain reaction (PCR). Specifically, one of such method frequently used in food industry to observe animal derived product fabrication is PCR restriction fragment length polymorphism (RFLP). PCR-RFLP is based on the comparison of the bands profile generated after certain enzymes digest the DNA target. PCR-RFLP is appropriate for meat testing due its ability in exploiting sequence variation in designated DNA region that allows species differentiation even from closely related species through DNA fragment restrictions selected by suitable restriction enzyme. PCR-RFLP is advantageous since it is simple, cheaper, and easier to be adjusted for routine big-scale studies such as surveillance program.

Ancaman terhadap Masuknya Virus Penyakit Mulut dan Kuku melalui Daging Ilegal di Perbatasan Darat Indonesia-Malaysia Risma Juniarti Silitonga; Retno Damayanti Soejoedono; Hadri Latif; Etih Sudarnika
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk menganalisis keberadaan daging ilegal di perbatasan darat Indonesia-Malaysia sebagai ancaman risiko masuknya virus PMK ke wilayah Indonesia. Data primer diambil menggunakan teknik pengumpulan pendapat pakar dengan kuisioner, wawancara mendalam (in-depth interview) dan pengamatan langsung di lapang. Data sekunder diperoleh dari publikasi ilmiah dan tulisan atau data yang tidak dipublikasi (statistik, dokumen dan laporan dari instansi berwenang). Penentuan responden secara purposive sampling. Hasil studi menunjukkan bahwa daging ilegal diperkirakan berasal dari berbagai negara termasuk dari negara/zona yang berstatus endemis PMK seperti Semenanjung Malaysia, Thailand, India dan negara/zona yang dinyatakan tidak diketahui oleh responden. Jenis daging ilegal yang masuk ke Entikong berisiko sebagai sumber infeksi PMK seperti daging beku bertulang tanpa limfoglandula dan jeroan beku tanpa limfoglandula. Berdasarkan jalur dan frekuensi pengangkutan, perkiraan volume pemasukan daging ilegal menunjukkan kemungkinan daging masih bisa lolos melalui jalur non-kendaraan. Kondisi-kondisi tersebut mengindikasikan bahwa pemasukan daging ilegal dapat sebagai ancaman risiko masuknya virus PMK ke Indonesia khususnya di perbatasan darat Indonesia-Malaysia, Entikong. Perlu dilakukan upaya pencegahan untuk mengurangi ancaman risiko yaitu dengan melakukan pengawasan yang lebih ketat di pintu-pintu pemasukan dengan koordinasi lintas instansi di perbatasan untuk bersama-sama mencegah pemasukan daging ilegal.

Tingkat Kejadian Escherichia coli Penghasil Extended Spectrum Β-Lactamase yang Diisolasi dari Feses Broiler di Kota Bogor Cholilia Abadiatul Masruroh; Mirnawati Bachrum Sudarwanto; Hadri Latif
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The use of antibiotics in food-producing animals may encourage the occurence of bacterial resistance named Escherichia coli that produces Extended Spectrum β-lactamase (ESBL). Antibiotical resistance is an important issue in the animal health and human health. This study focused on the presence of ESBL-producing E.coli in chicken feces. The number of sample feces was 100. E. coli isolates were obtained from broiler chicken feces in Bogor. Isolates obtained were identified using API 20E kit. Confirmation of ESBL in this study used Double disc method using antibiotical disc namely cefpodoksim, ceftazidime and cefotaxime. The existence ofESBL-producing E. coli which isolated from the feces of broiler chickens in the city of Bogor is 25% (4/16).

Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW) Rinaldi Ghurafa; Denny Widaya Lukman; Hadri Latif
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.

Phenotypic and Serotypic Characterization of Staphylococcus aureus Strains from Subclinical Mastitis Cattle (KARAKTERISASI SECARA FENOTIPE DAN SEROTIPE STAPHYLOCOCCUS AUREUS YANG BERASAL DARI MASTITIS SUBKLINIK PADA SAPI) Siti Gusti Ningrum; Wyanda Arnafia; Sylvia Oscarina; Retno Damajanti Soejoedono; Hadri Latif; Mohammad Ashraf; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.659 KB)

Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also studied, such as erythromycin, streptomycin, tetracycline, cefepime,nitrofurantoin, amikacin, chloramphenicol, and ciprofloxacin. About 78% of the isolated were moderatelysensitive to nitrofurantoin, while 89% were highly resistant to cefepime and ciprofloxacin. Using thevarious mammals’ sera, seven isolates out of 104 revealed the presence of Spa.

Residu Zeranol dalam Daging Sapi yang Diimpor dari Australia dan Selandia Baru Melalui Pelabuhan Tanjung Priok (ZERANOL RESIDUE IN BEEF MEAT IMPORTED FROM AUSTRALIA AND NEW ZEALAND THROUGH THE PORT OF TANJUNG PRIOK) Siti Khadijah; Hadri Latif; Agatha Winny Sanjaya
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.737 KB)

Zeranol is one of the synthetic growth hormone produced from mycotoxin that could affect humanhealth. The objective of this study was to determine zeranol residue in beef meat imported from Australiaand New Zealand using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed that zeranolresidue was detected in 5 of 59 meat samples (8,5 %) of Australia and 1 of 59 meat samples (1,7 %) of NewZealand, with mean concentration of 0,644±0,157 ppb and 0.680±0.00 ppb, respectively. There were nosignificant differences in the concentration of zeranol residue between the meat from both countries(p>0,005). In addition the concentration of zeranol residue was below the National Standardization Agencyof Indonesia Maximum Residue Limit (MRL) which is 2 ppb.

Komposisi Kimiawi dan Fraksinasi Protein Susu Kuda Sumba (THE CHEMICAL COMPOSITION AND PROTEIN FRACTIONATION OF SUMBA MARE’S MILK) Annytha Ina Rohi Detha; Mirnawati Sudarwanto; Hadri Latif; Frans Umbu Datta
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.579 KB)

The aims of this study were to determine both chemical composition and fraction of the proteincompounds of sumba mare’s milk. Determination of the chemical compositions of sumba mare’s milk havedone by analyzing protein content using the Kjeldahl method, fat content using Gerber method, lactosecontent and the total solids content. Identification of antimicrobial compounds of whey proteins in milkusing high performance liquid chromatography (HPLC) method. The results showed that the average ofsumba mare’s milk contained protein, fat, lactose and total solids were; 1.82%, 1.67%, 6.48% and 11.37%respectively. The average value of protein and fat in sumba mare’s milk was decrease significantly at fifthmonth of lactation period. Based on identification of antimicrobial compounds using HPLC method, thereare six main peaks with different polarities and retention times. In conclusion, sumba mare’s milk havea balance composition that can be used as a source of nutritious food and the milk likely also has six mainantimicrobial compounds in its whey protein.

Resistensi Antibiotik Terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat (ANTIBIOTIC RESISTANCE TO ESCHERICHIA. COLI ISOLATED FROM BROILER FARMS IN SUBANG DISTRICT, WEST JAVA PROVINCE) Aji Barbora Niasono; Hadri Latif; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic resistance in avian bacterial pathogens is a common problem in the Indonesia poultry industry. The present study aimed to provide information on the present status of antibiotic resistance in Escherichia coli (E. coli) from a broiler farm environment. The research method uses a cross sectional study with a stratified random sampling pattern. E. coli was collected from litter using boot swab method at the broiler house farms in Subang District. E. coli isolates (n = 74) were obtained from 74 samples of boot swabs. The sample was isolated and identified morphologically and biochemically using the IMVIC test to obtain E. coli isolates. The test was followed by a sensitivity test for nine antibiotics, namely tetracycline, sulfamethoxazole, trimethoprim, ampicillin, nalidixic acid, ciprofloxacin, enrofloxacin, gentamicin and chloramphenicol by using the agar dilution method. The interpretation of the results of this sensitivity test refers to the Clinical and Laboratory Standards Institute CLSI VET01S (2015) and CLSI M100 (2018). Measurement of the level of knowledge is done using a structured questionnaire that has been tested for validity and reliability. E. coli was isolated from 74 samples of boot swab. 93.2% of E. coli samples were found to be MDR with the highest prevalence in four types of antibiotics at 40.5%. The most common resistances observed were against tetracycline 97.3%, sulfamethoxazole 87.8%, trimethoprim 74.3%, ampicillin 68.9%, nalidixic acid 64.8%, ciprofloxacin 45.9%, enrofloxacin 40.5%, gentamicin 28.4% and chloramphenicol 10.8%. The findings of this study revealed the high level of antibiotic resistance in broiler environment is worrisome and have negative implications for human and animal health.

Co-Authors . Sumitro . Yudi AGATHA WINNY SANJAYA Agik Suprayogi Agustin Indrawati Ahmad, Hasniah Aji Barbora Niasono Annytha Ina Rohi Detha Asep Yayan Ruhyana B. Sudarwanto, Mirnawati Chaerul Basri Cholilia Abadiatul Masruroh Debby Fadhilah Pazra Dede Sri Wahyuni Denny Lukman Denny W. Lukman Denny Widaya Lukman Diyan Cahyaningsari Dwi Desmiyeni Putri Etih Sudarnika Fitri Hidayati Heru Susilo Heru Susilo Herwin Pisestiyani Herwin Pisestyani I wayan Teguh Wibawan Julia Rosmaya Riasari koekoeh santoso Lina Noviyanti Sutardi Loisa Loisa Loisa, Loisa Melani Wahyu Adiningsih Mirnawati B Sudarwanto Mirnawati B Sudarwanto Mirnawati B. Sudarwanto Mirnawati B. Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Bachrun Sudarwanto Mirnawati Sudarwanto Mohammad Ashraf Nadhear Nadadyanha Dannar Navasuriya Radha Krisnan Nugraha, Arifin Budiman Okti Nadia Poetri Platika Widiyani Qing Wen, Jyn Soong Rahmat Setya Adji Raphaella widiastuti Ratna Normaliska Retno Damajanti Soejoedono Retno Damayanti Soejoedono Rifky Danial Rinaldi Ghurafa Risma Juniarti Silitonga Rizal Eko Kurniawan Saimah Saimah Saimah, Saimah Siti Gusti Ningrum Siti Khadijah Sudarwanto, Mirnawati Bachrum Susanti, Oli Sylvia Oscarina Trioso Purnawarman Umi Cahyaningsih Undari, Fajar Okta Wahyudi, Ading Widiyani, Platika Wyanda Arnafia Yessy Anastasia Yunindika, Thufeil Yusuf Ridwan

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