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Cut Nila Thasmi
Fakultas Kedokteran HEwan Universitas Syiah Kuala, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Agripet

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Pengaruh Pemberian PGF2 Sebelum Koleksi terhadap Peningkatan Kualitas Semen dan Level Testosteron Sapi Aceh Eka Meutia Sari; Saifan Nur; Mulkan Mulkan; Gholib Gholib; Cut Nila Thasmi; Tongku Nizwan Siregar
Jurnal Agripet Vol 21, No 1 (2021): Volume 21, No. 1, April 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i1.17778

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian hormon PGF2 sebelum koleksi terhadap peningkatan kualitas semen dan level testosteron sapi Aceh di UPT. Hewan Coba Universitas Syiah Kuala. Penelitian ini dilaksanakan dari bulan Januari sampai dengan Maret 2019. Data hasil pemeriksaan kualitas semen dianalisis secara deskriptif. Dalam penelitian ini digunakan satu ekor sapi Aceh jantan sebagai kontrol dengan 2 perlakuan, yaitu P1 (tanpa pemberian PGF2 sebelum koleksi) dan P2 (pemberian PGF2 30 menit sebelum koleksi) dengan dosis 25 mg tiap perlakuan. Setiap perlakuan dilakukan sebanyak lima kali dan dilakukan secara bergantian setiap minggunya. Rata-rata volume (ml); konsentrasi (106 sel/ml); motilitas (%), viabilitas (%); abnormalitas (%); dan level testosteron (ng/ml) pada P1 vs P2 masing-masing adalah 5,21,30 vs 5,20,97 (P0,05); 145,436,58 vs 172,8 46,27 (P0,05); 63,876,44 vs 75,050,08 (P0,05); 69,300,22 vs 75,050,08 (P0,05); 16,900,08 vs 10,460,09 (P0,05); dan 28,0714,37 vs 24,196,11 (P0,05). Disimpulkan bahwa pemberian PGF2 30 menit sebelum koleksi dapat meningkatkan kualitas spermatozoa tetapi tidak dapat meningkatkan konsentrasi testosteron. (Case study the effect of giving pgf2 before the collection of the quality of Aceh cattle semen) ABSTRACT. This study aims to determine the effect of giving PGF2 hormone before collection to improve the quality of Acehnese semen in UPT. Hewan Coba Syiah Kuala University. This research was conducted from January to March 2019. Data on semen quality examination were analyzed descriptively. In this study, one Aceh cattle was used as a control with 2 treatments, namely P1 (without administration of PGF2 before collection) and P2 (administration of PGF2 30 minutes before collection) at a dose of 25 mg per treatment. Each treatment was carried out five times and carried out alternately every week. The results showed that the average volume of cement (ml) and pH P1 and P2 respectively 5.2 1.30 and 5.2 0.97, and 6.60.55 and 6.5 0.50. While the average concentration of spermatozoa (106 cells / ml) in P1 and P2 were 145.4 36.58 and 172.8 46.27 respectively. The average percentage (%) of progressive spermatozoa motility, circularity, non motility, viability, and abnormalities in P1 and P2 respectively: 63.87 6.44 and 73.63 9.42, 11.34 3, 70 and 9.0 7.01, 24.79 4.04 and 17.37 4.44, 69.30 0.22 and 75.05 0.08, 16.90 0.08 and 10.46 0.09. It was concluded that administration of PGF2 30 minutes before collection can increase the quality of spermatozoa but cannot increase the concentration of testosterone.
Isolasi dan Identifikasi Bakteri Gram Negatif pada Uterus Sapi Aceh yang Mengalami Repeat Breeding Indi Rafika; Cut Nila Thasmi; Herrialfian Herrialfian; Rosmaidar Rosmaidar; Hafizuddin Hafizuddin
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.17017

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui jenis bakteri Gram negatif pada uterus sapi Aceh yang mengalami kawin berulang. Dalam penelitian ini digunakan enam ekor sapi Aceh betina yang terdiri atas tiga ekor sapi fertil (K1) dan tiga ekor yang mengalami kawin berulang (K2, repeat breeding, RB). Pengambilan sampel bakteri dalam uterus sapi dilakukan dengan menggunakan swab steril yang disimpan dalam media nutrient broth (NB) untuk isolasi dan identifikasi jenis bakterinya. Berdasarkan hasil penelitian, baik K1 dan K2, positif terinfeksi bakteri dan diperoleh 16 isolat bakteri di antaranya enam isolat berasal dari K1 dan sepuluh isolat berasal dari K2. Dari 10 isolat bakteri Gram negatif yang ditemukan pada sapi RB terdiri atas Escherichia coli (E. coli) (30,00%), Pseudomonas sp. (30,00%), Enterobacter sp. (20,00%), Klebsiella sp. (20,00%). Disimpulkan bahwa bakteri yang paling banyak menginfeksi uterus sapi RB adalah E. coli dan Pseudomonas sp.(Isolation and identification of gram negative bacteria in uterine Aceh cow with repeat breeding) ABSTRACT. The study determined the type of Gram negative bacteria of the Aceh cattle uterus which has been diagnosed with repeat breeding. The study was conducted on six Aceh cows consists of 3 normal fertile cows (K1) and 3 repeat breeding cows (K2, RB). The bacteria in uterine samples were collected using sterile swab, kept into nutrient broth (NB) for further isolation and identification of bacteria. The result of the study showed that the total of 16 isolates of bacteria was observed in all cows : 6 isolates from group K1 and 10 isolates from group K2. A total of 10 isolates from cattle with repeat breeding consist of Escherichia coli (E. coli) (30.00%), Pseudomonas sp (30.00%), Enterobacter sp (20.0%), and Klebsiella sp (20.0%). It can be concluded that the most bacteria that infect uterine cows with repeat breeding are E. coli and Pseudomonas sp.
Co-Authors Abdullah Hamzah Arman Sayuti Awaluddin Awaluddin Azhar Azhar Budianto Panjaitan Dasrul Dasrul Dasrul Dasrul Dasrul Dasrul Dasrul Dasrul Denny Irmawati Dewi Ratna Sari Dian Masyitha Dina Maulidya Siregar Dwinna Aliza Eka Meutia Sari Eka Meutia Sari Elfi Satria Suryani Gholib Gholib Ginta Riady Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Herrialfian . Husnurrizal . Idawati Nasution Indi Rafika Indra Sitorus M Nur Salim M. Hasan M. Ikhsanuddin M. Isa Mauridatun Ramli Mefrianti Efendi Mudhita Zikkrullah Ritonga Mulkan Mulkan Mulyadi Adam Muslim Akmal Muslim Akmal Nazaruddin N Novi Afriani Nur Afriani Nur Nuzul Asmilia Priyo Sambodo Raja Nurul Huda Rasmaidar Rasmaidar Razali Daud Razali Razali Rosmaidar Rosmaidar Saifan Nur Satria Tanjung Sri Wahyuni Sri Wahyuni Sri Wahyuni Tongku Niswan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Triva Murtina Lubis Wahyuni, Sri Widya Zahara Winaruddin Winaruddin Yola Alifa Yudha Fahrimal Zamma Khasyarif