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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian Jurnal Hortikultura Indonesia (JHI) ACTA VETERINARIA INDONESIANA Jurnal Penyuluhan Media Gizi dan Keluarga Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Media Kesehatan Masyarakat Indonesia Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Medical Laboratory Technology Journal AcTion: Aceh Nutrition Journal Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Journal of Agribusiness and Local Wisdom Journal of International Conference Proceedings Jurnal Kedokteran Hewan
Muhamad Rizal Martua Damanik
Departemen Gizi Masyarakat, Fakultas Ekologi Manusia (FEMA), Institut Pertanian Bogor, Bogor 16680
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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THE ETHYL ACETATE FRACTION OF TORBANGUN (COLEUS AMBOINICUS L.) LEAVES INCREASING MILK PRODUCTION WITH UP-REGULATED GENES EXPRESSION OF PROLACTIN RECEPTOR Iwansyah, Ade Chandra; Damanik, Rizal Martua; Kustiyah, Lilik; Hanafi, Muhammad
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

This study aim was to determine lactagogue effect of torbangun leaves to plasma levels of lactogenic hormone and gene expression of their receptors in mammary glands of lactation rats. Lactagogue activity was evaluated by volume of milk was produced by the rats treated with commercial milk booster contained ?katuk' leaves extract (AF), ethyl acetate fraction of torbangun leaves (EA), water extraction of torbangun leaves (AQ) and kaempferol (KP). Lactating rats (n=5) of Sprague dawley with six pups were fed with AF, EA, AQ, and KP in the amount of 50 mg/kg, 30 mg/kg, 80 mg/kg and 60 mg/kg body weight, respectively. The feed was given orally every two days and starting from day 2 after giving birth until day 28. The volume of milk was estimated by the increment pup weight after breastfed. The levels of serum lactogenic hormones were determined by ELISA methods. Moreover, in order to measure the gene expression of the lactogenic hormone's receptors in the mammary gland a real time - reverse transcription polymerase chain reaction (RT-PCR) method was performed. The results showed that ethyl acetate fraction of torbangun leaves (EA) (a) was not significantly stimulating the synthesis of serum prolactin and estradiol at day 14 and day 28 lactation period, (b) down-regulated the gene expression of estradiol receptor (ER?) at day 28, and (c) up-regulated the gene expression of prolactin receptor (PRLR) in mammary gland at day 14 and day 28. This study indicated that ethyl acetate fraction of torbangun leaves was induced milk production, within up-regulated the gene expression of prolactin receptor (PRLR) in the mammary gland of lactation rats.
The Ethyl Acetate Fraction of Torbangun (Coleus amboinicus L.) Leaves Increasing Milk Production With Up-Regulated Genes Expression of Prolactin Receptor Ade Chandra Iwansyah; Rizal Martua Damanik; Lilik Kustiyah; Muhammad Hanafi
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aim was to determine lactagogue effect of torbangun leaves to plasma levels of lactogenic hormone and gene expression of their receptors in mammary glands of lactation rats. Lactagogue activity was evaluated by volume of milk was produced by the rats treated with commercial milk booster contained ‘katuk' leaves extract (AF), ethyl acetate fraction of torbangun leaves (EA), water extraction of torbangun leaves (AQ) and kaempferol (KP). Lactating rats (n=5) of Sprague dawley with six pups were fed with AF, EA, AQ, and KP in the amount of 50 mg/kg, 30 mg/kg, 80 mg/kg and 60 mg/kg body weight, respectively. The feed was given orally every two days and starting from day 2 after giving birth until day 28. The volume of milk was estimated by the increment pup weight after breastfed. The levels of serum lactogenic hormones were determined by ELISA methods. Moreover, in order to measure the gene expression of the lactogenic hormone's receptors in the mammary gland a real time - reverse transcription polymerase chain reaction (RT-PCR) method was performed. The results showed that ethyl acetate fraction of torbangun leaves (EA) (a) was not significantly stimulating the synthesis of serum prolactin and estradiol at day 14 and day 28 lactation period, (b) down-regulated the gene expression of estradiol receptor (ERα) at day 28, and (c) up-regulated the gene expression of prolactin receptor (PRLR) in mammary gland at day 14 and day 28. This study indicated that ethyl acetate fraction of torbangun leaves was induced milk production, within up-regulated the gene expression of prolactin receptor (PRLR) in the mammary gland of lactation rats.
Co-Authors . Triyani A.A. Ketut Agung Cahyawan W Abidah Nur Ade Chandra Iwansyah Ade Chandra Iwansyah Adhitya Aji Candra Adi, Fajar Ahmad Sulaeman Alfiah, Elma Amalia, Niken Rizki Anna Fatchiya Anwas, E. Oos M. Ardi Adji Aripin Ahmad Armina Puji Utari Arum Fitria Asri Sulistyawati Astri Ayu Novaria Aziz, Sandra A Budi Setiawan Budi Setiawan Budi Setiawan Clara M. Kusharto Clara M. Kusharto Dede Kusmana Devita Annisa Dewi Sukma Dian Ekawati Dias Indrasti Didik Hariyadi Dodik Briawan E. Oos M. Anwas, E. Oos Endang Warsiki Endang Warsiki dan Citra Dewi Wahyono Putri (E-Jurnal Agro-Industri Indonesia) Eny Rolenti Togatorop Eva Fitriyaningsih Evy Damayanthy Faisal Anwar Fitrah Reynaldi Hamidah, Hamidah Hardinsyah Hendrik Hendrik Hidayat Syarief Hidayat Syarief Husnah Husnah Iflan Naufal Ikeu Ekayanti Ikeu Tanziha Ilyatun Niswah Ita Djuwita ITA DJUWITA Izna Nurdianty Muhdar Junaidi Junaidi Karina Rahmadia Ekawidyani Katrin Roosita Kusai Kusai Leily Amalia Lilik Kustiyah Lilik N Yuliati Mahani Mahani Marisa Marisa Marissa Indreswari Marthin, Yodi Meilla Dwi Andrestian, Meilla Dwi Mokhamad Fahrudin Muhammad Hanafi Muhammad Hanafi Mulyana, Erik Mustafa Kamal Nancy Dewi Yuliana Nelly Marissa Nining Tyas Triatmaja Oktavianus Para Endro Ploeger, Angelika Pramadya Alfitra Pudji Muljono Renny Fridieyanti Rimbawan , Rimbawan R Risti Rosmiati Rusmiyati, Henny Sari, Dia Novita Sri Anna Marliyati Sri Yuliani Sukma, Dewi Sumali M Atmojo Syarifah Iis Aisyah Syuja' Rafiqi Tetty Herta Doloksaribu Teuku Muliadi Tiurma Sinaga Tjahja Muhamdri Utama, Lalu Juntra Vera Uripi Wahidin Wahidin Waras Nurcholis Wiwit Estuti