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Darminto .
Dept. Of Physics, Fac. Of Math. And Natural Sciences-Sepuluh November Institute Of Tech. Surabaya Kampus ITS Sukolilo, Surabaya 60111, Indonesia
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Journal : JURNAL BIOLOGI INDONESIA

 1 
Seroepidemiologi Nipah Virus pada Kalong dan Ternak Babi di Beberapa Wilayah di Indonesia Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.164 KB) | DOI: 10.14203/jbi.v5i1.3205

Abstract

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology
Pemetaan Genetik Virus Avian Influenza di Indonesia 2007 Dharmayanti, NLP Indi; Indriani, R.; Hartawan, R.; Hewajuli, D.A.; Ratnawati, A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.831 KB) | DOI: 10.14203/jbi.v5i2.3195

Abstract

ABSTRACTGenetic Mapping of Indonesian Avian Influenza Viruses 2007.Department of agricultureimplements vaccination as one a tool to control of avian influenza disease. The vaccinationprogram use virus seed such as H5N1, H5N2 and H5N9. Such as vaccination program for otherdiseases, avian influenza vaccine program have lack implementation in the field. In 2007,department of agriculture evaluated the AI vaccination program such as the master seedvaccine that can be used. Result of the evaluation showed that 11 of AI vaccines that weredistributed in Indonesia did not give protection more than 60% to Indonesian isolates in 2006(A/Ck/Pwt-Wij/06). From this point and many AI cases in the field in 2007, the aim of thisstudy was to conduct genetic diversity of avian influenza viruses which have circulated inIndonesia region. We used virus isolation for propagate the viruses, RT-PCR for identificationand DNA sequencing on HA1 region to analysis genetic diversity for genetic mapping anduseful for master seed candidate. The result of the study showed that there were 6 group ofgenetic diversity in 2007; Isolates from group 1, 5 and 6 can be used for AI vaccine candidate.Key words: genetic mapping, genetic diversity and avian influenza viruses
Kemampuan Primer General CK4/CK2 Virus Infectious Bronchitis (IBV) untuk Mengaplifikasi Genom IBV Isolat Lapang Indonesia Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
Potensi Virus A/Ck/West Java/PWT-Wij/2006 Sebagai Vaksin Indriani, R.; Dharmayanti, NLP; Adjid, R.M.A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.541 KB) | DOI: 10.14203/jbi.v7i2.3117

Abstract

ABSTRACTPotential virus A/Ck/West Java/PWT-Wij/06 for Vaccin. Vaccination program for controllingavian influenza (AI) virus infection in poultry have been emerged during the past 5 years inIndonesia. However, due to the mutation character of this virus the available vaccines were nolonger effective. Therefore a new local isolate of avian influenza virus A/Ck/West Java/Pwt-Wij/2006 was studied. The virus, formulated as inactive vaccine, was injected in to 3 weeks oldof layer chickens intramuscularly. At 3 weeks after vaccination, vaccinated chickens werechallenged against seven local isolates of HPAI H5N1 intranasaly. Unvaccinated chickenswere included in the challenge test as control. Results showed that the vaccine produced100% protection against A/Ck/West Java/Pwt-Wij/2006 (homologous), A/Ck/West Java/1067/2003, A/Ck/West Java/Hj-18/2007, and A/Ck/Payakumbuh/BPPVRII/2007; produced 90%protection against A/Ck/BB149/5/2007, and 80% protection against A/Ck/West Java/Hamd/2006 isolates. The vaccine also stoppped viral shedding by day 5 to 7 after challange. Thisstudy indicate that the new local isolate of avian influenza A/Ck/West Java/Pwt-Wij/2006 hasgood potency as vaccine with broad spectrum against a range of AI viruses available inIndonesia.Key words: avian influenza, HPAI, H5N1,vaccine, poultry.
SEROEPIDEMIOLOGI NIPAH VIRUS PADA KALONG DAN TERNAK BABI DI BEBERAPA WILAYAH DI INDONESIA Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3205

Abstract

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology
PEMETAAN GENETIK VIRUS AVIAN INFLUENZA DI INDONESIA 2007 Dharmayanti, NLP Indi; Indriani, R.; Hartawan, R.; Hewajuli, D.A.; Ratnawati, A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i2.3195

Abstract

ABSTRACTGenetic Mapping of Indonesian Avian Influenza Viruses 2007.Department of agricultureimplements vaccination as one a tool to control of avian influenza disease. The vaccinationprogram use virus seed such as H5N1, H5N2 and H5N9. Such as vaccination program for otherdiseases, avian influenza vaccine program have lack implementation in the field. In 2007,department of agriculture evaluated the AI vaccination program such as the master seedvaccine that can be used. Result of the evaluation showed that 11 of AI vaccines that weredistributed in Indonesia did not give protection more than 60% to Indonesian isolates in 2006(A/Ck/Pwt-Wij/06). From this point and many AI cases in the field in 2007, the aim of thisstudy was to conduct genetic diversity of avian influenza viruses which have circulated inIndonesia region. We used virus isolation for propagate the viruses, RT-PCR for identificationand DNA sequencing on HA1 region to analysis genetic diversity for genetic mapping anduseful for master seed candidate. The result of the study showed that there were 6 group ofgenetic diversity in 2007; Isolates from group 1, 5 and 6 can be used for AI vaccine candidate.Key words: genetic mapping, genetic diversity and avian influenza viruses
KEMAMPUAN PRIMER GENERAL CK4/CK2 VIRUS INFECTIOUS BRONCHITIS (IBV) UNTUK MENGAPLIFIKASI GENOM IBV ISOLAT LAPANG INDONESIA Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
POTENSI VIRUS A/CK/WEST JAVA/PWT-WIJ/2006 SEBAGAI VAKSIN Indriani, R.; Dharmayanti, NLP; Adjid, R.M.A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3117

Abstract

ABSTRACTPotential virus A/Ck/West Java/PWT-Wij/06 for Vaccin. Vaccination program for controllingavian influenza (AI) virus infection in poultry have been emerged during the past 5 years inIndonesia. However, due to the mutation character of this virus the available vaccines were nolonger effective. Therefore a new local isolate of avian influenza virus A/Ck/West Java/Pwt-Wij/2006 was studied. The virus, formulated as inactive vaccine, was injected in to 3 weeks oldof layer chickens intramuscularly. At 3 weeks after vaccination, vaccinated chickens werechallenged against seven local isolates of HPAI H5N1 intranasaly. Unvaccinated chickenswere included in the challenge test as control. Results showed that the vaccine produced100% protection against A/Ck/West Java/Pwt-Wij/2006 (homologous), A/Ck/West Java/1067/2003, A/Ck/West Java/Hj-18/2007, and A/Ck/Payakumbuh/BPPVRII/2007; produced 90%protection against A/Ck/BB149/5/2007, and 80% protection against A/Ck/West Java/Hamd/2006 isolates. The vaccine also stoppped viral shedding by day 5 to 7 after challange. Thisstudy indicate that the new local isolate of avian influenza A/Ck/West Java/Pwt-Wij/2006 hasgood potency as vaccine with broad spectrum against a range of AI viruses available inIndonesia.Key words: avian influenza, HPAI, H5N1,vaccine, poultry.
Co-Authors A Ratnawati A Wiyono Abdullah Shahab abdurrouf Abdurrouf Adler Haymans Manurung Agung Suwandaru Agus Wiyono Ahzan, Sukainil Alimuddin Ali Anne Zulfia Arifin, Rizal Bambang Subali Budhi Priyanto Budi Priyanto Cahyoingrat, Suryo E. Cahyono, Yoyok D.A Hewajuli Dadan Hamdani, Dadan Daniels, Peter Daniels, Peter Dedi Supriadi Dharmayanti, N.L P.I. Dharmayanti, N.L P.I. Dharmayanti, NLP Dharmayanti, NLP Eddy S Siradj Eko Handoyo Endang Siti Astuti Endhah Purwandari Fahmi Astuti, Fahmi Fatimah Karunia Sultera Field, Hume Field, Hume Firdaus, Aulia Anisa Gatut Yudoyono H Hamid Haniffudin Nurdiansah Hartawan, R. Hartawan, R. Helmy Hamid Hesti Irnanta Hewajuli, D.A. Hewajuli, D.A. I Wayan T. Wibawan Ida Ayu Putu Sri Widnyani Ida Widaningrum, Ida Indra Sidharta Indrawati Sendow Intifadhah, Sahara Hamas Iwan Dini Iwan Yahya Jagoda Ignjatovic Johari, Norhasnidawani Joni Dwi Pribadi Kholis Nurhanafi Kolif Yulianto L Parede Lee, Vannajan Sanghiran Lintang Edityastono Malik Anjelh Baqiya Manik, Tetti Novalina Martin Malole Mashuri Mashuri Mochammad Zainuri Morrissy, Chris Morrissy, Chris Muhammad Taqiyuddin Muhammad Zainuri Muharam Saepulloh Munir, Rahmawati Muzakki, Fikrun Najib N. Suryana N.L.P Indi Dharmayanti N.L.P.I Dhamaryanti N.L.P.I Dharmayanti N.L.P.I. Dharmayanti Ninis Hadi Haryanti, Ninis Hadi NLP Indi Dharmayanti Nugroho, Ferry Anggoro Ardy Nuni Widiarti Nurma Sari, Nurma Nurshabrina, Atiqa Taqiyya P Ronohardjo Prayogi, Saiful Prayogi, Soni Puspita, Niniek Fajar R Damayanti R Indriani R M A Adjid R. Indriani R.M Abdul Adjid R.M. Abdul Adjid R.M.A. Adjid Ratnawati, A. Ratnawati, A. Retno Asih, Retno RISA INDRIANI Risa Indryani Risdiana, Risdiana Rizoputra, Ivan Rizqy Wahyu Septiono S Hardjoutomo Saleh, Muh Santoso, Tanto Budi Shaliha, Shaliha Shinta Maharani Trivena Sholih, Ahmad Simon Sadok Siregar, Simon Sadok Simson Tarigan Siti Ragil Handayani Sitorus, Mido Ester Sjamsul Bahri SRI WARDANI Suarso, Eka Suhadak Suhadak Suminar Pratapa Suryajaya Suryajaya, Suryajaya Susan M Noor Susi Soviana Sutikno Sutikno Syah, Rakhasoni Firman T Sjafriati T Syafriati Taher Al habsji Tatty Syafriati Totok Wianto Upik Kesumawati Hadi W. Asmara W. T. Artama, W. T. Wahyudi, Sriati Winardi, Yoyok Zainul Arifin Zainuri, M Zulkarnain, Zulkarnain