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Journal : Berkala Penelitian Hayati

Quercetin, rutin, proanthocyanidin, catechin and epitacethin as fatty acid synthase inhibitor using virtual screening Candra Rini Hasanah Putri; Sutiman Bambang Sumitro; Setyawati Karyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.501 KB) | DOI: 10.23869/62

Abstract

Fatty acid synthase is known to participate in the occurrence of malignancies, so fatty acid synthase inhibition is expected can restrain malignancy. In this research, virtual screening is done by molecular docking between the active ingredients in Tamarindus indica with three domains of FAS. In thioesterase domain, it turns out all of the active ingredients in Tamarindus indica can bind thioesterase domain right in the place where orlistat (as a reference inhibitor) bonded, with a higher strength than the orlistat. In enoyl [acyl-carrier-protein] reductase domain, it turns out the binding affinity of quercetin, rutin, catechin and epicatechin against fatty acid synthase are greater than the reference inhibitor, triclosan. In malonyl-CoA / acetyl-CoA-ACP-transacylase domain, it turns out the binding affinity of quercetin, rutine, proanthocyanidin, and catechin against fatty acid synthase is greater than the natural substrate, Malonyl-CoA. The high binding affinity of the active ingredients in Tamarindus indica against the two domains of fatty acid synthase that may also can be occupied by reference inhibitors, showed the ability of Tamarindus indica as an inhibitor of fatty acid synthase. While the high ability of active ingredients in Tamarindus indica to bind to a domain that should be occupied by the natural substrate of fatty acid synthase (malonyl-CoA) demonstrated the ability of Tamarindus indica to inhibit fatty acid synthase's work in a way to compete with the natural substrate. This study shows that Tamarindus indica may serve as anti-malignancy through its ability to inhibit fatty acid synthase.
The UV-Vis spectrum of antioxidant complex from frozen-dried egg white protein and tomato extract Siti Imama Khoiriyah; Mohammad Mabrur; Sutiman Bambang Sumitro; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.22 KB) | DOI: 10.23869/110

Abstract

In this study, we want to characterize egg white protein-tomato extract complex based on the UV-Vis spectrum. The formulation of antioxidant complex Non-Freeze-drying (NFD) was made at a ratio of 1:10 (v/v) while the Freeze-drying (FD) sample was made at a ratio of 1:2 (v/v). The results showed that the absorption peak of FD complex profile value was lower than the single ingredient, while the tomato extract was higher than the complex profile. On the contrary, the NFD complex showed a higher peak absorption value than to every single ingredient. Based on each UV-Vis spectrum profile, the tomato extract profile shifted the absorption peak, and the absorbance value of the FD sample was changing higher than the NFD sample, while the egg white protein spectrum profile did not change significantly in the FD and NFD samples. The absorption peak of the complex is higher than the single ingredient indicated that the characterization of the complex had been formed. In the NFD complex profile, the absorption peaks formed at the wavelength regions of 230 nm and 280 nm with absorbance values of 2.51 and 0.76 and its number has higher than those of the FD complex with absorbance values of 2.47 and 1.82 at wavelengths of 220 and 265 nm.
HUMAN SPERM PROTEIN 116 KDA: A CANDIDATE ANTIGEN FOR IMMUNOCONTRACEPTION TECHNOLOGY Umi Lestari; Aulanni’am Aulanni’am; Basuki B. Purnomo; Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.499 KB) | DOI: 10.23869/139

Abstract

Spermatozoa membrane antigenic protein utilized as the substance candidate of immunocontraception has important criteria, that are only expressed in spermatozoa, cannot be found in the other tissue, and also should not have kinase activities. The observations done finally conclude 116kD, a sperm head’s protein is within the above criteria. It’s antigenic characters are shown with ability of it’s polyclonal antibody to bind the human spermatozoa, and interestingly this polyclonal antibody enable to block fertilization of other species in an in-vitro test experiments. This reseach also acquired that 116 kDa protein is specific and exists only in spermatozoa, and not in the other tissue such as spleen, kidney, pancreas, and epidydimic. This 116 kDa protein distribute along the whole area of the sperm, but mostly accumulated around the head up to the the neck. The imunohistological staining of the testis also indicate that this protein exists in the spermatid of the testes, but not be found in other somatic tissues, and it’s antibody is evidently was recognised by the goat and cow spermatozoa membrane protein resulted in blocking the fertilization of both species respectively. And the conclussion, 116 kD is Non-kinase protein specific only exists in the area of the head of human spermatozoa. It is therefore believed to be adequate candidate for antigen substance of immunocontraception technology.
SELF ASSEMBLY AND MAGNETISM OF LIVING BIOLOGICAL MOLECULES Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.515 KB) | DOI: 10.23869/162

Abstract

Biological molecules are essentially nano size structure. All of them are complex structure with specifi c function dedicated to perform normal ordered organizational system. The forces for their work are non-covalent interactions; include spontaneous folding of proteins, DNA, RNA and other bio-macromolecules, ligand-receptors interactions, assembly-disassembly of macromolecule, andtransportation or movement of many other nano size sub cellular components. The non-covalent interactions are weak bonds system that is low energetic chemical and physical forces. The energetic forces are mainly atomic forces such as electromagnetic force emergence from electron spinning and transitions at every atom of the complex macromolecular structure. The energy will work along with different level of energy, and atomic positioning within macromolecules. This paper review and discuss the role of magnetism on molecular working process as part of thermodynamically open systems to develop order, which is constantly receiving, transforming and dissipating energy, can and do continually exhibit self assembly and organization, along with the self repairing, and perpetuation.