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Kartika Sari Dewi
Fakultas Psikologi, Universitas Diponegoro
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Journal : HAYATI Journal of Biosciences

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Medium Optimization for Recombinant Human Papillomavirus Type 52 L1 Protein Production in Pichia pastoris GS115 Platform on Bioreactor Scale Mustopa, Apon Zaenal; Nur Amani, Febriyanti; Irawan, Herman; Novianti, Ela; Swasthikawati, Sri; Ekawati, Nurlaili; Nurfatwa, Maritsa; Joko Wahyono, Daniel; Juanssilfero, Ario Betha; Mamangkey, Jendri; Purnomo, Yudi; Hertati, Ai; Wijaya, Hans; Dewi, Kartika Sari; Ningrum, Ratih Asmana
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1283-1294

Abstract

Human papillomavirus (HPV) stands as the primary etiological agent in the development of invasive cervical cancer worldwide. The L1 protein is a pivotal constituent of prophylactic HPV vaccines. Notably, HPV type 52 is one of the most prevalent genotypes found in squamous cell carcinoma cases in Indonesia. This research endeavor aims to enhance the productivity of recombinant HPV-52 L1 protein by optimizing the culture conditions of P. pastoris GS115 cells. In this study, we conducted trials employing 17 different media variants to optimize the expression of recombinant HPV-52 L1 protein. The results from small-scale experiments revealed three media, namely SYN6.10, BMMY, and SYN6.1, which exhibited promising yields of recombinant HPV-52 L1 protein as assessed through ELISA or immunoassay analysis. We succeeded in refining the SYN6.10 derivative, denoted as SYN6.10b, specifically designed for use in 1-L and 5-L bioreactors. This achievement was realized by adjusting Trace Element Solution (TES) and Vitamin Solution (VS) concentrations and implementing a methanol fed-batch phase with the addition of 0.3% methanol after 24 and 48 hours of fermentation in the P. pastoris medium. Further visualizations through SDS-PAGE and western blot analysis confirmed the protein after 72 hours of fermentation in a 1-L bioreactor using the SYN6.10b medium. In conclusion, the SYN6.10b medium required a 72 hours fermentation period to successfully express recombinant HPV-52 L1 protein in the P. pastoris platform.
Co-Authors Achmad Fauzan Franzfabian Adriyan Wicaksono Afindaningtryas Warapsari Ahmad Nur Mizan Ai Hertati, Ai Amiruddin Anastasia Christie Silaen Anggita, Devina Annisa Mei Verticilla Apon Zaenal Mustopa Ario Betha Juanssilfero Arsanti Oktawati Suseno Asrul Muhamad Fuad, Asrul Muhamad Chairunnisa Erlan Cholila Shinta Jatisari Costrie G. Widayanti Costrie Ganes Widayanti Desvina, Della Devana Agripinata Dian Ratna Sawitri Dinda Putri Perdana Dwi Hersinta Putri Dzulhaq, Adefia Larasati Ekawati, Nurlaili ERLINA Ernasari Ernasari Ervina Safitri Evi Pujiyanti Fithria Wardanie Gesya Arsih Windhani Gilang Kartika Adi Perdana Ginanjar, Adriana Ginanjar, Adriana Soekandar Hadistia, Ananda Hafni Hilda Nafeesa Hapsari, Annita Dwi Hasoloan, Aswan Heny Herawati I Ketut Mahardika Indraswari, Tuti Indraswari, Tutri Irawan, Herman Isnanur Khurotul Aini Jendri Mamangkey Joko Wahyono, Daniel Karel K. Himawan, Karel K. Karyono Karyono, Lela TA Lestiyadi, Ayu Puspa Lestyadi, Ayu Puspa Livy, Sharyn Martono, Adi Mega Tala Harimukthi Nailul Fauziah Novandra, Rio Novianita Ayu Pramestuti Novianti, Ela Nugroho, Fentiny Nur Amani, Febriyanti Nurfatwa, Maritsa Pradipta Christy Pratiwi RATIH ASMANA NINGRUM Riswan Tarigan, Riswan Riyona Desvy Pratiwi, Riyona Desvy Ryani Nugrahwati Sandra Handayani Sutanto Sari, Shela Puspita Sari, Shella Puspita Sekar Paramitha Hanafi Sinta Dewi Parahita Siti Amalia Sri Handono Sri Lestari Sri Swasthikawati, Sri Suryono Suryono Teuku Fahmi Unika Prihatsanti Viddy Agustian Rosyidi Wijaya, Hans Yudi Purnomo Zanjabilla, Sabighoh