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All Journal PROSIDING SEMINAR NASIONAL Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Sainteknol : Jurnal Sains dan Teknologi Biosfer: Jurnal Tadris Biologi Bioeksperimen: Jurnal Penelitian Biologi Journal of Biology Education Biosaintifika: Journal of Biology & Biology Education Martabe : Jurnal Pengabdian Kepada Masyarakat Journal of Innovative Science Education Journal of Maternity Care and Reproductive Health Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) Borneo Nursing Journal (BNJ) JURNAL PENELITIAN PERAWAT PROFESIONAL Pasundan Food Technology Journal (PFTJ) Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Peduli Masyarakat PROFESI (Profesional Islam): Media Publikasi Penelitian Mattawang: Jurnal Pengabdian Masyarakat Jurnal Ilmiah Wahana Pendidikan ABDIMAS Jurnal Pengabdian Kepada Masyarakat Mejuajua Life Science Journal Of Human And Education (JAHE) Borneo Community Health Sevice Journal Journal Keperawatan SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT Jurnal Ekonomi, Teknologi dan Bisnis Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Bookchapter Alam Universitas Negeri Semarang Journal of Advanced Health Informatics Research Java Nursing Journal SmartComp TRIAGE GEMASSIKA: Jurnal Pengabdian kepada Masyarakat Unnes Science Education Journal Jurnal Ilmiah Kesehatan Keperawatan Bioeksperimen Jurnal Ilmiah Kesehatan Mandira Cendikia Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) Journal of Community Empowerment Life Science GANAPATI
Pramesti Dewi
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Journal : Bioeksperimen

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Isolation, Characterization, and Identification of Biosurfactant-Production Bacteria from Used Cooking Oil Waste Fisky Aditya Pratama; Pramesti Dewi; Ibnul Mubarok; Dewi Mustikaningtyas
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11115

Abstract

Waste cooking oil constitutes a significant environmental concern in Indonesia. It has the potential to be utilized as a substrate for biosurfactant-producing bacteria. Biosurfactants are amphiphilic compounds produced by microorganisms, containing both hydrophilic and hydrophobic components that enable them to dissolve in both water and fats, as well as to reduce surface tension. This study aims to isolate, characterize, and identify biosurfactant-producing bacteria from waste cooking oil. Isolation proccess was conducted using Mineral Salt Medium enriched with cooking oil as an inducer and Carboxy Methyl Cellulose as a carbon source and emulsifier. Two isolates were successfully obtained and purified. Their biosurfactant-producing ability was evaluated through drop collapse test and oil spreading test. One isolate demonstrated superior performance, showing positive activity in both tests, including a 0.7 cm oil spreading zone. Identification involved macroscopic observation, Gram staining, endospore staining, and a catalase test. The isolate exhibited characteristics of a Gram-positive, rod-shaped, spore-forming, catalase-positive bacterium with small, circular, milky-white colonies and a smooth surface. This identification is non-molecular and based on a phenotypic approach according to Bergey’s Manual for preliminary bacterial classification. Based on these features, the isolate is presumed to belong to the genus Bacillus, known for its robust biosurfactant production. The findings suggest that waste cooking oil can be a promising source of indigenous biosurfactant-producing bacteria, contributing to both microbial conservation and potential development of eco-friendly biotechnological products.
Co-Authors Adiratna Sekar Siwi Adystia, Yusdike Agus Prasetyo, Ahmad Ahmad Agus Prasetyo Anam, M. Khairul Ananda, Rahimma Artantia Andiniyati, Fitri Ari Yuniastuti Arvidhea Safira Gunawan Astrid Farmayati Atun Raudotul Ma'rifah Atun Raudotul Ma’rifah Atun Raudotul Ma’rifah Azzakiyah, Kholifah Nur Burhan, Asmat Cahyaningrum, Etika Dewi Danang Dwi Saputro Devi Nisa Hidayati Dewi Mustikaningtyas Dewi Mustikanintyas Dhimas Fajar Eka Purnama Dyah Rini Indriyanti Elawati, Nunung Eni Enni Suwarsi Rahayu Esti Isussilaning Setiawati Fadhiatul Mufrihah Fajarsari, Nurida A’sha Fauziah Hanum Nur Adriyani Felintina Yuniarti, Felintina Fisky Aditya Pratama Ghani, Farid Gunawan, Arvidhea Safira Hartini, Tuti Hasyim Abdurrasyid Hidayatullah, Riki Hilmi, Aanisah Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Indrati, Retno Indri Heri Susanti Isnaeni Karunia Annisa Khairul Anam Kirana, Swastika Nur La’ali, Shaimaa Alma Lestishiyami, Lesy Lina Herlina Lintang Suryani, Roro Lisdiana Lisdiana Made Suandika Maria Ayu Puspita Mariah Ulfah Martien Herna Susanti Maya Safitri Maya Safitri meidina rahmawati Melinda, Aura Tirsa Murniati Nindia Sefi Utami Ning Setiati Ningrum, Ema Wahyu Noor Rochmah Ida Ayu Trisno Putri Noor Yunida Triana Nur Rohmah Tria Romadhoni Opty Prastika, Luvi Prapto Sasongko, Didik Priyantini Widiyaningrum Purwono Purwono, Purwono Puspita Diana Arumawati rahmawati, meidina Rahmaya Nova Handayani Rakhmasari, Pramesti Regita Ramadani, Mutiara Bintang Reni Dwi Setyaningsih Retno Indrati Retno Sri Iswari Ria Millati Ria Millati Rusuli, Alvin Maulana Sadikin, Nadya Audina Nurkhafiya Safar Dwi Kurniawan Sardjono Sardjono Sardjono Sardjono Sekar Dwi Ardianti Sigit Saptono Silvia Nurbaiti, Silvia Siti Haniyah Siti Harnina Bintari Siti Wahidatun Nisa Soniawati, Diah Steffani Fransisca Suci Khasanah Sugiarta, Nyoman Sulasfiana Alfi Raida, Sulasfiana Alfi Sunyoto Sunyoto SURTININGSIH, SURTININGSIH Suryani, Roro Lintang Susanto, Amin Suwarsi Rahayu, Enni Syamsul Aryanto Talitha Widiatningrum Tin Utami Tri Retno Nurasih Tri Sumarni Tri Yudono, Danang Triana, Noor Yunida Tyas Agung Pribadi, Tyas Agung Utami, Pambajeng Walandipa, Ghani Wibowo, Tophan Heri Wirakhmi, Ikit Netra Woung-Ru Tang Wulan Christijanti Yance Anas Yati, Ernallah Yusdike Adystia Yustinus Ulung Anggraito Zahwa, Iana Zulfa Rofiqoh