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All Journal PROSIDING SEMINAR NASIONAL Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Sainteknol : Jurnal Sains dan Teknologi Biosfer: Jurnal Tadris Biologi Bioeksperimen: Jurnal Penelitian Biologi Journal of Biology Education Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education Martabe : Jurnal Pengabdian Kepada Masyarakat Journal of Innovative Science Education Journal of Maternity Care and Reproductive Health Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) Borneo Nursing Journal (BNJ) JURNAL PENELITIAN PERAWAT PROFESIONAL Pasundan Food Technology Journal (PFTJ) Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Peduli Masyarakat PROFESI (Profesional Islam): Media Publikasi Penelitian Mattawang: Jurnal Pengabdian Masyarakat Jurnal Ilmiah Wahana Pendidikan Mejuajua Life Science Journal Of Human And Education (JAHE) Borneo Community Health Sevice Journal Journal Keperawatan SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT Jurnal Ekonomi, Teknologi dan Bisnis Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Bookchapter Alam Universitas Negeri Semarang Journal of Advanced Health Informatics Research Java Nursing Journal GEMASSIKA: Jurnal Pengabdian kepada Masyarakat Unnes Science Education Journal Jurnal Ilmiah Kesehatan Mandira Cendikia Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) Journal of Community Empowerment Life Science GANAPATI
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Journal : Life Science

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Pengaruh Variasi Metode Ekstraksi Metabolit Sekunder Bakteri Endofit Daun Rambutan (Nephelium lappaceum L.) terhadap Pertumbuhan Escherichia coli dan Bacillus subtilis Hasyim Abdurrasyid; Ibnul Mubarok; Dewi Mustikaningtyas; Pramesti Dewi
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.2327

Abstract

Rambutan (Nephelium lappaceum L.) is one of the many tropical plants found in Indonesia. The leaves of the rambutan plant are known to be used as medicine, so they have the potential to be a source of endophytic bacteria that can produce beneficial compound. This study aims to isolate, identify of endophytic bacteria from rambutan leaves, and test their activity as an antibacterial with two different secondary metabolite extraction methods, namely maceration with 70% methanol and without maceration. This study used experimental research with a Completely Ramdomized Design (CRD). Isolation of endophytic bacteria from rambutan leaves produced two isolates (D1 and D2). The results of identification through macroscopic, microscopic, and biochemical test showed that two pure isolates had similarities to genus Bacillus sp. Antibacterial activity testing was carried out using well diffusion method. Based on the results of antibacterial test showed that extraction method with 70% methanol produced an extract that was more effective as an antibacterial with average diameter of clear zone formed for Escherichia coli bacteria were 13.55 mm (Isolate D1) and 12.13 mm (Isolate D2) and that of Bacillus subtilis bacteria were 16.27 mm (Isolate D1) and 19.69 mm (Isolate D2). This finding contributes to the fact that the type of secondary metabolite extraction method of rambutan leaf endophytic bacteria influences the sensivity as an antibacterial.
Pengaruh Lama Penyimpanan Biji Kopi Liberika pada Suhu Kamar terhadap Mutu Mikrobiologis dan Kadar Okratoksin A: The Effect of Storage Time of Liberica Coffee Beans at Room Temperature on Microbiological Quality and Ochratoxin A Levels Ramadani, Mutiara Bintang; Dewi Mustikaningtyas; Siti Harnina Bintari; Pramesti Dewi
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.5810

Abstract

Liberica coffee is one of the leading commodities in several regions so it has quite high economic potential. Coffee beans can be stored for quite a long time, more than one year, even more than 4 years before going through the roasting process. However, during long storage periods coffee beans can be attacked by microorganisms such as mold (ochratoxigenic and non-ochratoxigenic). This research aims to determine the effect of storage time on the quality of Liberica coffee beans, both microbiologically and chemically. The benefit of this research is to provide information on the quality of Liberica coffee beans. This research is a laboratory experimental research. The long storage time for coffee beans was 36 and 39 weeks. The results of the research showed that the yeast mold rate (AKK) on Liberica coffee beans stored for 39 weeks was greater than those stored for 36 weeks. The increase in AKK during storage is thought to be due to the presence of insects, temperature and humidity factors. A total of three mold isolates were found at week 36 and there were six mold isolates at week 39. The percentage of fungal isolate attacks at both weeks 36 and 39 was dominated by fungi of the Mucorales order (Mucor sp. and Absidia sp.). In the 36th week the attack of Mucor sp. amounted to 36.67% while in the 39th week the Mucor sp attack. 20%. and Absidia sp. 23.33%. Ochratoxin A (OTA) levels from week 36 to week 39 did not increase but instead decreased. This occurs due to OTA degradation by ochratoxin-degrading molds which are thought to be Aspergillus sp. during storage.
Antibacterial Activity Test of the Combination of Bay Leaf (Syzygium polyanthum) Extract and Nanochitosan Against Escherichia coli and Bacillus subtilis Astrid Farmayati; Siti Harnina Bintari; Pramesti Dewi; Dewi Mustikaningtyas
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.24777

Abstract

Indonesia is one of the world’s most biodiverse countries, including in medicinal plants that contain active compounds with potential as sources of modern medicine, particularly for treating bacterial infections. The use of herbal plants as antibacterial agents offers an alternative to overcome antibiotic resistance and the side effects of synthetic drugs. Bay leaf (Syzygium polyanthum) and nanochitosan are known for their antibacterial properties due to their bioactive compounds. However, studies on their combined effects are still limited. This study aimed to evaluate the antibacterial effectiveness of combinations of bay leaf extract (15%, 25%, and 35%) and nanochitosan (0.5% and 1%) against Escherichia coli and Bacillus subtilis. A Completely Randomized Design (CRD) was applied, using sterile distilled water as a negative control and 0.1% chloramphenicol as a positive control. The antibacterial activity test was performed using the Kirby-Bauer disk diffusion method. Results showed the positive control produced inhibition zones of 25.45 ± 1.27 mm for E. coli and 25.05 ± 0.39 mm for B. subtilis. The most effective combination was K3 (35% bay leaf extract and 0.5% nanochitosan), which produced inhibition zones of 12.65 ± 0.52 mm and 12.91 ± 0.55 mm, respectively. Statistical analysis using the Tukey HSD post hoc test confirmed significant differences among treatments. These findings suggest that the combination of bay leaf extract and nanochitosan holds promise as an alternative antibacterial agent
Comparison of the Rate and Level of Biodegradation of Leaf Waste by Lignocellulolytic Molds from Universitas Negeri Semarang Campus Walandipa, Ghani; Mustikaningtyas, Dewi; Widiatningrum, Talitha; Dewi, Pramesti
Life Science Vol. 14 No. 2 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i2.25828

Abstract

Biodegradation is a physical decomposition of the substrate caused by the activity of microorganisms by producing products that have benefits for humans. Lignocellulose degrading fungi are microorganisms that play a role in the process of decomposing organic waste. The biodegradation rate of each type of lignocellulose degrading mold is different. The purpose of this research is to comprehensively analyze and compare the rate and level of biodegradation of leaf waste in lignocellulolytic molds isolated from the campus environment of Universitas Negeri Semarang, as well as to identify and evaluate the type of lignocellulolytic mold that shows the highest efficiency in the rate and level of biodegradation of leaf waste. The method used in this research is quantitative method, by measuring the mass ratio and pH of leaf waste media before inoculation and after inoculation. The results of this study are the rate of leaf waste biodegradation based on the ratio of leaf waste mass by Trichoderma koningiopsis mold isolate of 0.015 grams/day, while by Trichoderma erinaceum mold isolate of 0.002 grams/day. Then the rate of leaf waste biodegradation based on the pH change value of leaf waste by Trichoderma koningiopsis mold isolate is higher with a pH change value of 0.3 when compared to the Trichoderma erinaceum mold isolate with a pH change value of 0.1. The superior performance of Trichoderma koningiopsis highlights its potential application in optimizing organic waste decomposition systems and provides a scientific basis for selecting lignocellulolytic fungi in sustainable waste management programs.
Co-Authors Adiratna Sekar Siwi Adystia, Yusdike Agus Prasetyo, Ahmad Ahmad Agus Prasetyo Anam, M. Khairul Ananda, Rahimma Artantia Andiniyati, Fitri Ari Yuniastuti Arvidhea Safira Gunawan Astrid Farmayati Atun Raudotul Ma'rifah Atun Raudotul Ma’rifah Atun Raudotul Ma’rifah Azzakiyah, Kholifah Nur Burhan, Asmat Cahyaningrum, Etika Dewi Danang Dwi Saputro Devi Nisa Hidayati Dewi Mustikaningtyas Dewi Mustikanintyas Dhimas Fajar Eka Purnama Dyah Rini Indriyanti Elawati, Nunung Eni Enni Suwarsi Rahayu Esti Isussilaning Setiawati Fadhiatul Mufrihah Fajarsari, Nurida A’sha Fauziah Hanum Nur Adriyani Felintina Yuniarti, Felintina Ghani, Farid Gunawan, Arvidhea Safira Hartini, Tuti Hasyim Abdurrasyid Hidayatullah, Riki Hilmi, Aanisah Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Indrati, Retno Indri Heri Susanti Isnaeni Karunia Annisa Khairul Anam Kirana, Swastika Nur La’ali, Shaimaa Alma Lestishiyami, Lesy Lina Herlina Lintang Suryani, Roro Lisdiana Lisdiana Made Suandika Maria Ayu Puspita Mariah Ulfah Martien Herna Susanti Maya Safitri Maya Safitri meidina rahmawati Melinda, Aura Tirsa Murniati Nindia Sefi Utami Ning Setiati Noor Rochmah Ida Ayu Trisno Putri Noor Yunida Triana Nur Rohmah Tria Romadhoni Opty Prastika, Luvi Prapto Sasongko, Didik Priyantini Widiyaningrum Purwaningsih, Indri Puspita Diana Arumawati rahmawati, meidina Rahmaya Nova Handayani Rakhmasari, Pramesti Regita Ramadani, Mutiara Bintang Reni Dwi Setyaningsih Retno Indrati Retno Sri Iswari Ria Millati Ria Millati Rusuli, Alvin Maulana Sadikin, Nadya Audina Nurkhafiya Sardjono Sardjono Sardjono Sardjono Sekar Dwi Ardianti Sigit Saptono Silvia Nurbaiti, Silvia Siti Haniyah Siti Harnina Bintari Siti Wahidatun Nisa Soniawati, Diah Steffani Fransisca Suci Khasanah Sugiarta, Nyoman Sulasfiana Alfi Raida, Sulasfiana Alfi Sunyoto Sunyoto SURTININGSIH, SURTININGSIH Suryani, Roro Lintang Susanto, Amin Suwarsi Rahayu, Enni Syamsul Aryanto Talitha Widiatningrum Tin Utami Tri Retno Nurasih Tri Sumarni Tri Yudono, Danang Triana, Noor Yunida Tyas Agung Pribadi, Tyas Agung Utami, Pambajeng Walandipa, Ghani Wardana, Irfan Ihza Wibowo, Tophan Heri Wirakhmi, Ikit Netra Woung-Ru Tang Wulan Christijanti Yance Anas Yati, Ernallah Yusdike Adystia Yustinus Ulung Anggraito Zahwa, Iana Zulfa Rofiqoh