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Abdi Dharma
Universitas Andalas
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Journal : Microbiology Indonesia

 1 
Selection and Identification of Cellulase-Producing Bacteria Isolated from the Litter of Mountain and Swampy Forest . WIZNA; HAFIL ABBAS; YOSE RIZAL; ABDI DHARMA; I PUTU KOMPIANG
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.383 KB) | DOI: 10.5454/mi.1.3.7

Abstract

The isolation and selection of cellulase-producing bacteria was conducted to identify the species of cellulolytic Bacillus. The bacteria were isolated from the litter of swampy forest in Pesisir Selatan and mountain forest in Lembah Anai Tanah Datar. These bacteria were cultivated in selective media to obtain bacteria from the genus Bacillus. Six Bacillus isolates were obtainedfrom swampy forest and three Bacillus isolates from mountain forest. These isolates were cultivated in agar medium with carboxymethylcellulose as the carbon source. Colonies which produced clear zones were assumed to be cellulolytic Bacillus. Based on biochemical and morphological examinations the result indicated that these two isolates were Bacillus coagulans and B. amyloliquefaciens. The cellulase activity of B. coagulans and B. amyloliquefaciens were 0.812 and 1 200 unit ml-1 to C1(b-exoglucanase) respectively, 0.368 and 0.488 unit ml-1 to Cx(b-endoglucanase) respectively.
Characterization of Extracellular Chitinase from Bacterial Isolate 99 and Enterobacter sp. G-1 from Matsue City, Japan MARIA ENDO MAHATA; ABDI DHARMA; IRSAN RYANTO; YOSE RIZAL
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.725 KB) | DOI: 10.5454/mi.2.1.7

Abstract

One hundred and twenty isolates of chitosanase producing bacteria were screened from water and soil from localies around Matsue city, Japan. In previous experiments, four isolates (isolates 96, 97, 99, and 100 strain ) were analyzed for their chitosanase characteristics, and one of the isolates (99) was detected as being both a chitosanase and a chitinase producer. Characteristics of the chitinase enzyme were analyzed in this study. Chitinase from bacterial isolate 99 showed higher activities compared to that Enterobacter sp. G-1 (isolated from water in Matsue city, Japan), the activity was 0.039 U/ml and the specific activity was 0.56 U/mg protein, while those from Enterobacter sp. G-1 were 0.029 U/ml and 0.48 U/mg protein respectively. Chitinase from isolate 99 was stable in a pH range between 4-7, while that from Enterobacter sp. G-1 was stable in pH range 3-7. Optimum pH of the chitinase produced by isolate 99 was 5 whereas the chitinase from Enterobacter sp. G-1 it was pH 7. Chitinase from isolate 99 was stable at temperature 20-60°C, while that from Enterobacter sp. G-1 at 20-50°C. Chitinase secreted by isolate 99 showed optimum temperature of 50°C while chitinase from Enterobacter sp. G-1 was optimal at 40°C. Several ions (Fe2+, Ba2+, Co2+) increased the activity of the enzyme from isolate 99 whereas Ca2+ and Co2+ increased activity of the Enterobacter sp. G-1 chitinase..
Co-Authors - Afrizal Admin Alief Admin Alif Admin Alif Afriyanti Azhar Alif, Admin Amir Mahmud Husein, Amir Mahmud Andika, Ahmad Zaki Andrian Anggela Marta Tasman Arif Juliari Kusnanda Arjon Turnip Armaini - Baharuddin Shaleh Barus, Samuelta Bayu Afnovandra Perdana Candra, Windy Chandra, Angelia Ayu Chandra, Suwito Christnatalis Chrysia, Celine Crispin, Andrian Reinaldo Dedi Nofiandi Delima Sitanggang, Delima Dewantoro, Rico Wijaya Dicky DS, Jumri Habbeyb Edbert, Edbert Edison Munaf Edison Munaf Elida Mardiah Eri Sulyanti Eti Farda Husin Eti Farda Husin Giawa, Priskila Veronika Hafil Abbas HAFIL ABBAS Harvianti, Yuniar Hazli Nurdin Heyneker, Daniel Husni Mukhtar I PUTU KOMPIANG I. P. Kompiang Indah Indah Indrawati - Indrawati Indrawati IRSAN RYANTO Jabang Nurdin Jamsari Jamsari Jefika, Meliy Kamble, Pratik Bibhisan Kosasi, Hendrick Laoli, Vincensius Yaatulo Lazuardya , Kevin Lee Wah Lim Luis, Matthew MARBUN, ADVENT TORAS Mardi Turnip, Mardi MARIA ENDO MAHATA Marniati Salim Mawaddah Harahap, Mawaddah Melona Siska Musifa, Eva Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhamidah Oktaf Rina Oktoriza, Ghifarizka Pasaribu, Beriman Periadnadi - Periadnadi Periadnadi Prayogi, Gali PULUNGAN, JURMIDA PURBA, JOICE ANGELINA Putra, Adya Zizwan Rahmadani Wulandari Rahmatika Yani Rahmiana Zein Randi, Albert Refilda Refilda Riska Hernandi Romaito pane, Jesi Saragi, Yosua Morales Sekatresna, Widiyanti Shaleh, Baharuddin Silaban, Elvin Josafat Simbolon, Billy Sirbu, Loredana Ioana Siregar, Yakob Mulia Siti Aisyah Siti Hajjir, Siti Sulyanti, Eri Sulyanti, Eri Sumaryati Syukur Sunnia, Cecilia Suryani Suryani Syafriza Yanti Syafrizayanti, Syafrizayanti Syafrizayanti, Syafrizayanti Syafrizayanti, Syafrizayanti Syukri Arief Talib, Ramanisa Muliani Tania, Alinda Tarigan, Julio Putra Telaumbanua, Christina Angelina Toyohide Takeuchi TRIMURTI HABAZAR Turnip, Josua Presen Vagga, Cherry Piya Valentino, Bue Vanness, Jeff Veron, Veron Victor Trismanjaya Hulu Wahida Nia Elfiza Warni, Mega Waruwu, Jefrin Widiyanti Sekatresna Wijaya, Davin Wijaya, Eko Bambang Wijaya, Jeremy Wijaya, Kenrick Alvaro Wijaya, Vincent William Wizna (Wizna) Wulandari, Rahmadani Yetria Rilda Yohana Samosir, Devi Yose Rizal Yoserizal Yoserizal Yunazar Manjang Yunazar Manjang Yunazar Manjang Zulkarnain Chaidir