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All Journal Nexus Pendidikan Kedokteran dan Kesehatan Nexus Kedokteran Komunitas Nexus Kedokteran Klinik Nexus Biomedika Jurnal Gizi dan Kesehatan Journal of Epidemiology and Public Health Indonesian Journal of Medicine Journal of Health Policy and Management Journal of Health Promotion and Behavior Journal of Maternal and Child Health Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Farmasi
RUBEN DHARMAWAN
Faculty of Medicine, Sebelas Maret University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

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MODIFIKASI PRINSIP PEMERIKSAAN β-D-GLUCAN UNTUK MENDETEKSI CANDIDA ALBICANS DALAM SERUM Dharmawan, Ruben; Darukutni, Darukutni; Haryati, Sri; Murkati, Murkati; Sari, Yulia; Prasetyo, Afiono Agung
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.419

Abstract

Candida albicans β-D-glucans examination has been used for invasive fungal detection in human blood and was approved by the Food and Drug Administration. However, the method is rarely used in Indonesia and also because the cost is hardly affordable. A modification of the method using enzymatic reaction is hoped will provide a simple and affordable measurement in human blood serum. β-D-glucans as heterogeneous molecules constitute the major carbohydrates fractions of cell wall and readily detected in supernatants of Candida albicans cultures are hydrolyzed by β glucanase to form D-glucose. This additional glucose is measured using Megazyme GOPOD-Format Procedure® at 510 nm. Candida albicans were identified and cultured was derived from a patient of Dr. Moewardi General Hospital, Surakarta in July, 2012. The results show that β-D-glucans from Candida albicans is measureable to the amount of μg/100 μL serum using this modification principle
Effects of Chronic Disease Control Club on Physical Activity, Anxiety, and Blood Sugar Level: A Path Analysis Evidence Putra, Fajar Alam; Indarto, Dono; Dharmawan, Ruben
Journal of Health Promotion and Behavior Vol 4, No 2 (2019)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Diabetes mellitus (DM) is a serious chronic disease with a tendency to worsen. The number of DM cases worldwide in 2015 was 415 million people and is expected to increase by 642 million cases in 2040. Indonesia ranks 7th in the world with 10 million DM cases in 2015, around 90% of which are Diabetes Mellitus Type 2. This study aimed to determine the effects of chronic disease control club on physical activity, anxiety, and blood sugar level.Subjects and Method: This was a cross sectional study conducted at Sukoharjo health center, Sukoharjo, Central Java, from August to September 2018. A sample of 245 type 2 DM patients was selected for this study. The independent variables were physical activity, anxiety, chronic disease control club, and complications. Data were collected using HARS, physical activity, complication and club activitiesquestionnaires. Data were analyzed by using path analysis.Results: Anxiety directly increased with com­plication (b= 12.55; 95% CI= 111.98 to 13.90; p<0.001). Anxiety directly de­cre­ased with chronic disease control club (b= -3.29; 95% CI= -4.72 to -1.85; p<0.001). Fasting blood sugar directly incre­ased with anxiety (b= 2.78; 95% CI= 1.32 to 4.24; p<0.001) and complication (b= 33.14; 95% CI= 8.40 to 57.87; p= 0.009). Fasting blood sugar directly decre­ased with physical activity (b= -15.12; 95% CI= -19.89 to -10.35; p<0.001) and chro­nic disease control club (b= -30.14; 95% CI= -48.80 to -11.48; p= 0.002).Conclusion: Anxiety directly increases with com­plication. Anxiety directly de­cre­ases with chronic disease control club. Fasting blood sugar directly incre­ases with anxiety and compli­cation. Fasting blood sugar directly decre­ases with physical activity and chro­nic disease control club.Keywords: blood sugar level, physical activity, anxiety, chronic disease control clubCorrespondence: Fajar Alam Putra. Masters Program in Public Health, Universitas Sebelas Maret. Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java. Email: bolodewe88@gmail.comJournal of Health Promotion and Behavior (2019), 4(3): 137-144https://doi.org/10.26911/thejhpb.2019.04.02.06
Geographical Satellite and Survey Data for Prediction of Dengue Cases in Sukoharjo, Indonesia Kusumawati, Dyah; Prayitno, Adi; Dharmawan, Ruben
Journal of Epidemiology and Public Health Vol. 1 No. 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Dengue fever is a disease based on environment and still a health problem. Problems related to the dengue fever vector distribution factor in terms of the spread of vector space with the use of geographic data and survey data in order to predict the incidence of dengue in the region.Subjects and Methods: This study used analytic observational with cross sectional approach using modeling Geographical Information Systems (GIS). The sampling technique in this research is saturated sampling of secondary data Sukoharjo District Health Profile in 2011-2014, population data and data Geographic, then all the data were analyzed using multiple linear regression.Results: There is a positive relationship between the area per Km2 with the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI -0.01 - 0:02; p = 0.310). There is a positive relationship between population density per soul / Km2dengan number of new cases of dengue fever, a significant relationship between population density with DHF cases. (B = <0:01; CI <0:01 to 0:01; p = 0.013). There is a negative relationship between topography per masl by the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI -0.02 - 0:01; p = 0.335). There is a positive correlation between rainfall per mm / yr with the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI <0:01 to 0:01; p = 0101). There is a positive relationship between river flow per ha by the number of new cases of dengue fever, although the relationship was not statistically significant. (B = 0:02; CI -0.01 - 0:03; p = 0318). There is a negative correlation between% Non Flick figure by the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI -0.02 - 0:01; p = 0764).Conclusions: The increase in land area, population density, rainfall, river flow is predicted to affect the increase in dengue cases, whereas the increase ABJ predicted topography and affecting the decline of dengue cases in the district of Sukoharjo in 2011-2014.Keywords: geographical data and survey data, prediction of dengue casesCorrespondence:
DETEKSI VIRUS DEN3 PADA SEMUA STADIUM Aedes aegypti (TRANSOVARIAL) DENGAN TEKNIK IMUNOSITOKIMIA SECARA INVITRO Kumoro Setya, Adhi; Dharmawan, Ruben
Jurnal Farmasi (Journal of Pharmacy) Vol. 5 No. 1 (2016): Jurnal Farmasi (Journal of Pharmacy), October 2016
Publisher : Jurnal Farmasi (Journal of Pharmacy)

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Abstract

Many dangerous parasites transmitted by the mosquito, one of which is dengue virus which causes dengue fever. The previous study said that in immunocytochemistry, dengue virus can be found in A. aegypti that does not suck blood. The basic principle of this examination was done by a technique of determining the existence (location) of antigen in cells in a tissue using antigen-antibody reaction. The purpose of this study was to determine the result of positive value of A. aegypti breeding stages in laboratory scale. DEN3 virus was mixed into the blood for trial then infected orally on the A. aegypti for breeding. All stages of breeding results was tested for DEN3 virus from the body tissue. DEN3 antigen in the body of the vector will bind with specific antibody from reagent and by the presence of the enzyme and chromogen substrate, it will give a brown color to the cells and granule surrounding the cells. Such color reaction was checked using a light microscope with 400x magnification with the positive control of DEN3 breed and the negative control of normal blood as a comparison. The findings showed that immunocytochemistry with SBPC method was capable of detecting DEN3 at all stages of metamorphosis of A. aegypti with the results of the stage respectively; egg: 82%, larva: 89%, pupa: 94% and adult: 100%. These results showed how immunocytochemistry technique as an alternative method which is relatively cheaper than molecular technique can be used for surveillance on dengue will appear.
Co-Authors -, Muryanti . Soetrisno . Suhanantyo A.A. Ketut Agung Cahyawan W Adi Prayitno Afiono Agung Prasetyo Anggraeni, Fatimah Dewi Argyo Demartoto, Argyo Arinto Hadi, Arinto Aris Sudiyanto Asri Sukawati Putri Atika Puspita Hapsari Atma Sanggani Tunikasari Aviria Ermamilia Bhisma Murti Darukutni, Darukutni Dono Indarto Dyah Kusumawati Dyah Mustikaningtyas Dewanti Endang Sutisna Sulaeman Eti Poncorini Pamungkasari Fajar Alam Putra, Fajar Alam Febrika Devi Nanda Hastuti, Nunik Maya Hervindita Dinda Siswandwika, Hervindita Dinda I Gusti Bagus Wiksuana Icha Dithyana Ike Nurrochmawati Irene Yunita Prihandini JB. Suparyatmo Suparyatmo, JB. Suparyatmo Kharisma Setya Harnani Krisna Rendi Awalludin Kumoro Setya, Adhi Mardiatmi Susilohati Mashuri, Yusuf Ari Mira Rizki Ramadhan, Mira Rizki Murkati, Murkati Narzah, Norma Arista Ningrum, Dhian Nurayni Sulistyo Okid Parama Astirin Pawito -, Pawito Pradana, Riska Chandra Purwanto - Rahardjo, Setyo Sri Rahmah, Anindita Hasniati Rasyiid, Astika Sofi Ariani Sofiyani, Maya Sri Haryati Sugiarto S Supriyadi Hari Respati Syarifah Syarifah, Syarifah Tamaulina Br Sembiring Umi Nadhiroh Wati, Emita Dewi Lilis Angkasa winarni - yoseph Indrayanto