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All Journal Hemera Zoa Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Kedokteran Hewan
I Ketut Puja
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Asosiasi Keragaman Lokus DNA Mikrosatelit DRB3 Gen Bola dengan Berat Badan Induk dan Berat Lahir Pedet pada Sapi Bali Ni Nyoman Trinayani; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

Genomic selection has a high potency to improve the genetic quality of Bali cattle. In effort to find out a molecular marker or DNA segment which is linked with a certain phenotype, we examined the association between BoLa-DRB3 microsatellite polymorphism and dam body weight and birth weight of calves in Bali cattle. A total of 40 dams from Abuan Village, the District of Susut was investigated. The total DNA was extracted with QIAamp DNA blood mini kit. The locus was amplified using PCR technique with a 54OC annealing temperature.. Alleles were separated through PAGE 6%, and visualized with silver staining. We found 10 alleles which their length varied from 145 bp to 233 bp. Allele 195 had the highest frequency that was 35.0%. The HE, HO, PIC were 0.821, 0.875, and 0.791 consecutively. Statistical analysis showed that the polymorphism of DRB3 microsatellite had a marginally significant association (p < 0.1) to the dam body weight. However, it had no significant association to the birth weight of the calves. The results of the research reflect that the DRB3-BoLA gene has a weak potency in controlling the body weight.
Hubungan Frekuensi dan Waktu Kawin dengan Jumlah Anak Sekelahiran pada Anjing Golden Retriever yang Dipelihara Di Kota Denpasar Tessa Yuli Oktaviami; Desak Nyoman Dewi Indira Laksmi; I Ketut Puja
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Abstract

This retrospective study was conducted to know the relation between time and frequency of mating on litter size of Golden Retriver  in Denpasar Region. Mating incident records of 19 Golden Retrievers from 8 kennels were compiled to be analyzed. These data can be use as a reference in effort to maximize the amount of  Golden Retriever clans. The result showed that the three times of mating on the 10th , 12th and 14th day after onset of proestrus produced the most litter size 10 ± 2.82. However, mating twice on wice on 9th and 11th after onset of proestrus produced a litter size of 8.50 ± 0.70. The statistic analysis depicted that there was no significant relationship between mating frequencies and mating time on litter size.
Polimosfisme Lokus Mikrosatelit BM1329 dan Hubungannya dengan Calving Interval pada Sapi Bali Made Raysa Merliana; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle plays an important role in supplying beef in Indonesia because it has good quality, high fertilization and low fat percentage. The aim of this research was to characterize the Bali cattle genetic using molecular marker of microsatellite BM1329 and its relation with calving interval. The data was taken from 19 cattles that belong to UD Sari Laba, Bangli, Bali. The association between BM1329 marker and calving interval was analyzed using General Linear Models concept. The result showed that the duration of calving interval for the Bali cattle was 314 – 451 days. There were 4 alleles found in the locus of microsatellite BM1329. The alleles size varied from 144 to 150 bp, in which, the allele 146 had the highest frequency (71.05%). The observed heterozigosity (Ho) and the expected heterozigosity (He) and the PIC were 0.474, 0.478, and 0.435 respectively. The statistical analysis showed that microsatellite BM1329 has no assocition with the calving interval (P>0.05).
The Effect of Soyabean Extender on Viability and DNA Integrity of Kintamani Dog Sperm on Cold Storage I Made Yoga Windu Pradana; Wayan Bebas; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

The objective of this study was to evaluate the viability and integrity of canine semen diluted in egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. Semen was collected by manual manipulation from an apparently healthy kintamani dog stud. The semen was subjected to gross and microscopic examination to determine its viability following dilution in different extenders. Only semen with motility rate of 60% or higher was used in this study. Semen samples were diluted in Tris-citrate-glucose-soya bean and egg yolk glucose-citrate extenders at two levels of dilution; sperm to extenders ratio of 1:2 and 1:3. Following semen dilution, evaluations were made on sperm motility, percentage of live sperm, and DNA integrity at 0 hour, 3 hours, and 6 hours under 5oC of storage. Data was analyzed by multivariate analysis of variance (MANOVA).The present results showed a high significant different (p<0.01) in sperm motility, percentage of live and DNA integrity between treatment with egg yolk glucose-citrate and Tris-citrate-glucose-soya bean.  The result also showed that percentage of live sperm and DNA integrity of semen at 1:2 dilution difference significantly (p<0.01) to that of 1:3 dilution. The sperm viability and integrity were significantly difference (p<0,01) between the duration of storage at 5°C. Thus, the current study indicated that Tris-citrate-glucose- soya bean extenders  was sufficient to maintain motility, viability, and DNA integrity of kintamani dog  spermatozoa during storage at 5 oC. Further research should be conducted to evaluate the fertility of spermatozoa following dilution with these extenders.
Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus) I Ketut Suardita; I Ketut Puja; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus). This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD) Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals
Asosiasi Lokus Mikrosatelit BMS1282 dengan Kejadian Kawin Berulang pada Sapi Bali Yesy Febnica Dewi; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle has an important role to fulfill the meat demands in Indonesia. The improvement of molecular technology will facilitated bali cattle genomic selection. The aim of this research was to verify association between BMS1282 microsatellite locus and repeat breeding in bali cattle. Totalling 52 bali cattle blood samples was taken from UD Sari Laba, Bangli, Bali as DNA resources. DNA was extracted using blood mini Kit qiagen and microsatellite locus was amplified by Polymerase Chain Reaction (PCR). Alleles were separated with 6% PAGE and visualized using silver stain. There were 5 alleles in BMS1282 locus which the alleles size varied from 148 to 156 bp. Allele 152 was the higest frequency (54.81%) and allele 156 was the lowest frequency (1.92%). The value of heterozygosity (Ho), He, and PIC was 0.346, 0.626, and 0.569. consecutively. Statistical analysis revealed that BMS1282 locus has no assosiation with the repeat breeding (P>0.05). This result indicates that BMS 1282 locus has no important role in regulating the repeat breeding.
Prevalence of Ear and Eye Disorders in Kintamani Dog Ni Made Devityasih Perayadhista; I Ketut Suatha; I Ketut Puja
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

This observational study was undertaken to evaluate the prevalence of ear and eye disorders in Kintamanidog. All data were gathered from registers of veterinary clinic located at Denpasar, Badung, and Gianyar.Various aspects concerning ear and eye disorders were collected by questionnaires. The total of Kintamanidog recorded from 2009 until 2013was 477 dogs. The result of this study showed that the prevalence ofexophthalmos (protrusion of the eye ball) and glaucoma were rare (0%), the cataract was occurred in onedog (0.2%), otitis was occurred in two dogs (0.4%), and conjunctivitis was occurred in three dogs (0.6%).All Kintamani dogs with ear and eye disorders were the male and thier ages were more than 12 months. Inconclusion, this study indicates  that the Kintamani dog has a lower problem of ear and eye disorders. In thefuture, this low prevalence of the problem hopefully can be maintained or even decreased by giving goodbreeding management.
Coconut Water Based Extender Effects on Motility,Viability, and DNA Integrity of Chilled Kintamani Dog Semen I Wayan Nico Fajar Gunawan; I Made Kardena; I Ketut Suatha; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 50C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p<0.01), whereas it had no effect on the percentage of dead sperm (p> 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.
Prevalence of Musculoskeletal Disorders in Kintamani Dog Deborah Bianti Purwanto; Ni Nyoman Werdi Susari; I Ketut Puja
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

Kintamani dog is the emerging breed of dog from Bali Indonesia. The study was conducted to determinethe prevalence of the type of musculoskeletal disorders in Kintamani dogs based on the data presented atmany veterinary clinics in Bali during a period of five years from January 2009 to December 2013. A totalof 475 samples was obtained and their variations in prevalence were analyzed on the basis of age and sex.The prevalence of musculoskeletal disorders in Kintamani dogs included accidental wound (3.2%), arthritis(1.3%), lameness (0.8%), nail injury (0.2%), and hip dysplasia (0.0%). Based on this study we concludedthat Kintamani dog could be expected not susceptible to musculoskeletal disorders.
Seleksi Awal Pejantan Sapi Bali Berbasis Uji Performans Ni Wayan Patmawati; Ni Nyoman Trinayani; Mahmud Siswanto; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Abstract

Performance test is a preliminary selection to choose Bali cattle stud based on qualitative and quantitative characteristics, including measurement, weighing and observation. Performance test is  applied  on rapid growth of bull  (about 1 year of age) by comparing individual performance with the performance of the group average. The number of bulls that were subjected  the  Performance test  in 2011  was 61  bulls.  Based on Performance test, the best three of the performances  namely the score of 113.30, 109.46,and 104.2  were s selected to be potential stud candidates for next Zuriat test (progeny test).
Co-Authors . Mutawadiah Anak Agung Gde Oka Dharmayuda Claudia Murti Andari Deborah Bianti Purwanto Desak Nyoman Dewi Indira Laksmi Evayana, Made Fania, Bella Glantiga, I Gede Jaya Rama Gunawan, I Wayan Nico Fajar I Gede Hendi Saputra I Gusti Agung Arta Putra I Gusti Ngurah Bagus Trilaksana I Ketut Berata I Ketut Suardita I Ketut Suatha I Ketut Wirata I Made Dwinata I Made Kardena I Made Sukada I Made Yoga Windu Pradana i Nengah Wandia I Nyoman Dibia I Nyoman Sulabda I Wayan Bebas Ida Bagus Made Oka IGNA Wisnu Adi Saputra IVAN M TARIGAN Jennifer Hood Kadek Ari Sindawati Lontoh, Rudy Luh Made Sudimartini Made Raysa Merliana Mahmud Siswanto Mahmud Siswanto Manuela, Ni Luh Maria Maliga Vernandes Sasadara Mufa, Romy Muhammad Dary Ni Komang Sri Puspaningsih Ni Made Devityasih Perayadhista Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Werdi Susari Ni Putu Vidia Tiara Timur Ni Putu VT Timur Ni Wayan Patmawati Ni Wayan Patmawati Ni Wayan Patmawati Nyoman S Dharmawan Nyoman Sadra Dharmawan Putu Ayu Sisyawati Putriningsih Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Suastika Rudy Lontoh Siswanto - Siswanto Suastika , Putu Sukernayasa, I Wayan Tessa Yuli Oktaviami Tjok Gde Oka Pemayun TJOKORDA ISTRI AGUNG Cintya Dalem Trilaksana, IGN Yesy Febnica Dewi ZAKIATUN MUHAMMAD