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All Journal Hemera Zoa Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Kedokteran Hewan

I Ketut Puja

Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 2538057

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Perilaku Bermasalah pada AnjingKintamani I Wayan Nico Fajar; I Made Sukada; I Ketut Puja
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.224 KB)

Anjing Kintamani adalah sebutan sekelompok anjing yang habitat aslinya di daerahKintamani. Penampilan dan karaketristik yang menarik menyebabkan anjing Kintamanisangat populer sebagai hewan kesayangan dan sekarang sedang diajukan ke FederationCynologique Internationale untuk penetapan sebagai anjing ras. Tujuan penelitian iniadalah mengevaluasi perilaku bermasalah pada anjing Kintamani. Penelitian ini dilakukandari bulan April sampai Mei 2011. Beberapa aspek yang berkaitan dengan masalah perilakudikumpulkan dengan menggunakan quesioner. Sebanyak 46 ekor anjing dari 75 anjing yangdigunakan sebagai sampel menunjukkan perilaku bermasalah (61.3%) dan 29 anjing tidakmenunjukkan perilaku bermasalah (38.7%). Di antara anjing yang digunakan sebagaisampel rata-rata umur anjing adalah 1- 2tahun dan hampir semua anjing belum disterilkan(92%). Juga didapat bahwa pemilik anjing memelihara anjingnya di halaman rumah (36%).Anjing berturut turt menunjukkan suara berlebihan (36%), perilaku merusak (17%), responberlebihan (6,7%), perilaku tidak pantas (34%) dan perilaku agresive (10,7%). Hasilpenelitian ini mendukung pendapat bahwa anjing kintamani tidak mempunyai perilakubermasalah, sehingga diharapkan sifat anjing Kintamani ini tetap dapat dipertahankan danuntuk dijadikan standar perilaku pada anjing kintamani

Faktor-Faktor yang Mempengaruhi Tidak Tercapainya Target Pelaksanaan Inseminasi Buatan pada Upsus Siwab di Kabupaten Jembrana Ni Komang Sri Puspaningsih; Gusti Ngurah Bagus Trilaksana; I Ketut Puja
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Bali cattle are a type of beef cattle that are widely developed in Indonesia because of their superiority. UPSUS SIWAB is an effort to accelerate the increase of livestock populations by intensifying the breeding of female cattle by utilizing artificial insemination technology. Based on the artificial insemination’s rate of success, Jembrana Regency has 31.87% the success rate of artificial insemination which is not optimal. This study aimed to determine the factors that influence the failure to achieve the target of artificial insemination at UPSUS SIWAB in Jembrana Regency. The method that used to collecting data in this study is by using a questionnaire and analyzed by analysis of factors. The results of this study are used the breeding variables, always called the officiers, and knowing the benefit of artificial insemination is main factors that influence the failure to achieve the target of artificial insemination in Jembrana Regency. Counseling need to be made about the benefit of artificial insemination so that more breeders can accept the existence of this technology and they can achieve the target of implementing artificial insemination in Jembrana Regency specially.

PERCENTAGE OF VIABLE SPERMATOZOA COLLECTED FROM THE EPIDIDYMES OF DEATH LOCAL DOG I Nyoman Sulabda; I Ketut Puja
Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purpose of this study to determine the effectof post mortem time on percentage of lifeepididymessperm from postmortem dog caudae epididymides. A total of 9 dog were usedand divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6hours postmortem. This way, samples were obtained at different times postmortem. Spermwere extracted from the caudae epididymes by means of cuts.The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33 ± 5.60(T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life wasaffected by postmortem time. There was significant decrease life sperm recovered fromepididymes postmortem (P<0.01). In conclusion, epididymes sperm from dog undergodecrease of percentage of life, but it could stay acceptable within many hours postmortem.We intepreted these data to indicate that it may still be possible to obtain viablespermatozoa many hours later.

PENGARUH SUBSTITUSI AIR KELAPA MUDA DENGAN PENGENCER SITRAT KUNING TELUR TERHADAP MOTILITAS DAN PERSENTASE HIDUP SPERMATOZOA ANJING I Nyoman Sulabda; I Ketut Puja
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purpose of this study was to determine the effect of tender coconut water substitutionon egg yolk citrate diluent with different doses on local breeds dog sperm motility and livespermatozoa. Semen was manually collected. Progressive motility and percentage of livespermatozoa were evaluated under a microscope utilizing a drop of semen between awarmed glass slide and coverslip, both at a temperature of 38 0C. The percentage of motileand live spermatozoa were examined by counting 100 spermatozoa using the classificationof Christiansen (1984). Sperm viability was assessed by eosinnegrosin staining. The result showed that coconut water substitution has significant effect on the motility and livespermatozoa . Combination between the levels of coconut water in the egg yolk citratediluent could be applied as an alternative diligent instead of egg yolk diligent for dogsemen up to 75%.

Peran Manajemen Populasi Anjing dalam Pemberantasan Rabies: Studi Kasus di Desa Pejeng, Kecamatan Tampaksiring, Kabupaten Gianyar, Provinsi Bali Kadek Ari Sindawati; I Ketut Puja; I Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Since 2008, rabies is still endemic in Bali. Dog Population Management is an effort to stabilize the dog population consisting of six components: education, legislation, identification and registration, vaccination, sterilization, and waste management in eradicating rabies. This program was implemented in Pejeng Village, Tampaksiring District, Gianyar Regency, Bali Province in November 2016, funded by Food and Agriculture Organization. This study aims to determine changes in knowledge, attitudes and ways of raising dogs in the community in the village of Pejeng after the application of Dog Population Management. This observational study uses a cross-sectional study through observation and dissemination of questionnaires directly to the field. The sampling technique uses probability sampling, a proportional stratified sampling technique with the number of samples was 313 respondents. It was conducted in November 2016 (before the Dog Population Management was implemented) and in September 2018 after the Dog Population Management was applied). The collected data were analyzed descriptively. The results before and after the application of Dog Population Management were analyzed by non-parametric analysis using the Wilcoxon test. Based on the results of this study, it was concluded that the Application of Dog Population Management significantly (P<0,05) could change knowledge, attitudes and ways of raising dogs in the community in Pejeng Village better than before.

Anjing Kintamani sebagai Model Pada Penelitian Biomedik: Aspek Hematologi (THE KINTAMANI DOGS AS A MODEL FOR BIOMEDICAL RESEARCH : HEMOTOLOGICAL ASPECTS) I Ketut Puja
Jurnal Veteriner Vol 1 No 1 (2000)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Anjing Kintamani sebagai Model Pada Penelitian Biomedik: Aspek Hematologi (THE KINTAMANI DOGS AS A MODEL FOR BIOMEDICAL RESEARCH : HEMOTOLOGICAL ASPECTS)

Profil Hormon Progesteron pada Anjing Kintamani Fase Anestrus Setelah Disuntik Pregnant Mare Serum Gonadotrophin (THE PROGESTERONE PROFILE IN ANESTRUS KINTAMANI BITCHES AFTER INJECTED USING PREGNANT MARE SERUM GONADOTROPIN) I Ketut Puja
Jurnal Veteriner Vol 2 No 2 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Polimorfisme Genetik DNA Mikrosatellite GEN BoLA Lokus DRB3 pada Sapi Bali (Bos indicus) I Ketut Puja; I Nengah Wandia; Putu Suastika; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.116

Tujuan penelitian ini adalah untuk mendapatkan informasi dasar mengenai distribusi frekuensi lokus DRB3 gen BoLa (bovine lymphocyte antigen) pada sapi Bali. Untuk isolasi DNA digunakan sampel darah sapi Bali yang diambil dari populasi sapi Bali yang berasal dari Bali dan sapi Bali yang berasal dari Nusa Penida. Jumlah sampel untuk sapi Bali yang berasal dari Bali adalah 22 ekor dan sapi yang berasal dari Nusa Penida 21 ekor. Jumlah allel lokus DRB3 pada sapi Bali asal Bali adalah 7 dan 9 allel dari sapi Bali asal Nusa Penida. Rataan heterozigositas perlokus adalah 0,7967 pada sapi Bali asal Nusa Penida dan 0,7863 pada sapi Bali asal Bali. Nilai PIC lokus DRB3 pada sapi Bali asal Nusa Penida adalah 0,7417 dan 0,742 pada sapi Bali asal Bali. Dapat disimpulkan dari hasil penelitian ini adalah lokus DRB3 pada sapi Bali sangat polimorfik.

Karakteristik Mikrosatelit Gen BoLA dengan Penanda Primer RM 185 pada Sapi Bali (Bos indicus) di Nusa Penida Putu Suastika; I Ketut Puja; I Nengah Wandia; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i2.131

Penelitian ini bertujuan menjelaskan distribusi frekuensi lokus gen bovine lymphocyte antigen (BoLA) memakai primer RM 185 pada sapi bali dari Nusa Penida. Sebanyak 21 ekor sapi bali yang berasal dari Nusa Penida diambil secara acak. Jumlah alel gen BoLA pada lokus RM 185 ada 7 macam alel yaitu 76 pb, 84 pb, 86 pb, 90 pb, 98 pb, 100 pb, dan 104 pb. Frekuensi tiap- tiap alel yang teramati adalah 0,02%; 0,09%; 0,31%; 0,07%; 0,12%; 0,28%; dan 0,09%. Rata- rata heterozigositas yang didapat adalah 0,804. Dapat disimpulkan, bahwa lokus RM 185 pada gen BoLA sapi bali di Nusa Penida adalah sangat polimorfik di antara populasi.

Karakterisasi Genetik Anjing Kintamani Menggunakan Petanda Mikrosatelit I Ketut Puja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2546

Karakterisasi molekuler anjing Kintamani berperan penting dalam penentuan status trah dan mencegah penurunan kemurnian akibat perkawinan silang dengan trah anjing lain. Anjing Kintamani sangat popular di Bali. Anjing Kintamani dipercaya berasal dari Kintamani, Bangli, tetapi asal usulnya sampai kini belum jelas. Mikrosatelit merupakan runutan nukleotida terulang yang didistribusikan secara acak dalam gen vertebrata. Lokus mikrosatelit ini telah diketahui sangat polimorfik, karena keragaman jumlah nukleotida yang terulang. Mikrosatelit merupakan petanda allel utama yang dapat digunakan untuk pemetaan gen, genetika populasi dan identifikasi individu serta alat untuk mengungkap karakter genetik hewan. Pada penelitian ini diungkap karakteristik genetik anjing Kintamani menggunakan mikrosatelit. Jumlah dan ukuran allel pada 425 anjing Kintamani dianalisis menggunakan 116 macam primer mikrosatelit. DNA diisolasi dari sel hasil swab pipi. Amplifikasi 116 lokus mikrosatelit menggunakan PCR dalam 12 multiplek. Produk PCR dipisahkan dengan gel bis-akrilamid 6% dalam automated DNA sequencer. Flurosesnsi yang dihasilkan dideteksi dengan program Genescan 3.1, dan program Strand versi 2.2.39 digunakan untuk menghitung jumlah allel. Hasil penelitian menunjukkan bahwa jumlah allel yang didapat adalah 1128. Jumlah allel perlokus berkisar antara 3 (AHT136) sampai 41 (FH2138). Rataan PIC adalah 0,68 dan semua lokus bersifat polimorfik.

Co-Authors . Mutawadiah Anak Agung Gde Oka Dharmayuda Claudia Murti Andari Deborah Bianti Purwanto Desak Nyoman Dewi Indira Laksmi Evayana, Made Fania, Bella Glantiga, I Gede Jaya Rama Gunawan, I Wayan Nico Fajar I Gede Hendi Saputra I Gusti Agung Arta Putra I Gusti Ngurah Bagus Trilaksana I Ketut Berata I Ketut Suardita I Ketut Suatha I Ketut Wirata I Made Dwinata I Made Kardena I Made Sukada I Made Yoga Windu Pradana i Nengah Wandia I Nyoman Dibia I Nyoman Sulabda I Wayan Bebas Ida Bagus Made Oka IGNA Wisnu Adi Saputra IVAN M TARIGAN Jennifer Hood Kadek Ari Sindawati Lontoh, Rudy Luh Made Sudimartini Made Raysa Merliana Mahmud Siswanto Mahmud Siswanto Manuela, Ni Luh Maria Maliga Vernandes Sasadara Mufa, Romy Muhammad Dary Ni Komang Sri Puspaningsih Ni Made Devityasih Perayadhista Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Werdi Susari Ni Putu Vidia Tiara Timur Ni Putu VT Timur Ni Wayan Patmawati Ni Wayan Patmawati Ni Wayan Patmawati Nyoman S Dharmawan Nyoman Sadra Dharmawan Putu Ayu Sisyawati Putriningsih Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Suastika Rudy Lontoh Siswanto - Siswanto Suastika , Putu Sukernayasa, I Wayan Tessa Yuli Oktaviami Tjok Gde Oka Pemayun TJOKORDA ISTRI AGUNG Cintya Dalem Trilaksana, IGN Yesy Febnica Dewi ZAKIATUN MUHAMMAD

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