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All Journal Hemera Zoa Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Kedokteran Hewan
I Ketut Puja
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Keberhasilan Inseminasi Buatan (IB) Pada Sapi Bali di Kecamatan Mengwi, Badung, Bali Fania, Bella; Trilaksana, I Gusti Ngurah Bagus; Puja, I Ketut
Indonesia Medicus Veterinus Vol 9 (2) 2020
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.68 KB) | DOI: 10.19087/imv.2020.9.2.177

Abstract

Inseminasi buatan (IB) merupakan suatu rangkain proses terencana dan terpogram karena menyangkut kualitas genetik ternak di masa yang akan datang.. Penelitian ini bertujuan untuk mengetahui keberhasilan IB dan faktor yang berpengaruh terhadap keberhasilan inseminasi buatan pada sapi bali di Mengwi. Jenis penelitian yang dilaksanakan yaitu penelitian observasional dengan rancangan crossectional data diambil dalam suatu waktu di Mengwi, Badung, Bali. Pengumpulan data dalam penelitian yaitu teknik pemeliharaan data diambil menggunakan kuisioner, dan untuk pengambilan data pelaksanaan diambil dari ISIKHNAS. Hasil penelitian dapat disimpulkan bahwa inseminasi buatan di Mengwi, sudah berhasil dan faktor peternak yang berpengaruh terhadap keberhasilan inseminasi buatan di Mengwi, terdiri dari pengetahuan inseminasi buatan, pengetahuan keunggulan inseminasi buatan, pengetahuan tentang kapan sapi dikawinkan, sedangkan faktor petugas inseminasi buatan seluruh faktor saling mempengaruhi terhadap keberhasilan inseminasi buatan di Mengwi, Badung, Bali.
Karakterisasi Genetik Anjing Kintamani Menggunakan Petanda Mikrosatelit Puja, I Ketut
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.2546

Abstract

Karakterisasi molekuler anjing Kintamani berperan penting dalam penentuan status trah dan mencegah penurunan kemurnian akibat perkawinan silang dengan trah anjing lain. Anjing Kintamani sangat popular di Bali. Anjing Kintamani dipercaya berasal dari Kintamani, Bangli, tetapi asal usulnya sampai kini belum jelas. Mikrosatelit merupakan runutan nukleotida terulang yang didistribusikan secara acak dalam gen vertebrata. Lokus mikrosatelit ini telah diketahui sangat polimorfik, karena keragaman jumlah nukleotida yang terulang. Mikrosatelit merupakan petanda allel utama yang dapat digunakan untuk pemetaan gen, genetika populasi dan identifikasi individu serta alat untuk mengungkap karakter genetik hewan. Pada penelitian ini diungkap karakteristik genetik anjing Kintamani menggunakan mikrosatelit. Jumlah dan ukuran allel pada 425 anjing Kintamani dianalisis menggunakan 116 macam primer mikrosatelit. DNA diisolasi dari sel hasil swab pipi. Amplifikasi 116 lokus mikrosatelit menggunakan PCR dalam 12 multiplek. Produk PCR dipisahkan dengan gel bis-akrilamid 6% dalam automated DNA sequencer. Flurosesnsi yang dihasilkan dideteksi dengan program Genescan 3.1, dan program Strand versi 2.2.39 digunakan untuk menghitung jumlah allel. Hasil penelitian menunjukkan bahwa jumlah allel yang didapat adalah 1128. Jumlah allel perlokus berkisar antara 3 (AHT136) sampai 41 (FH2138). Rataan PIC adalah 0,68 dan semua lokus bersifat polimorfik.
VPH-1 Turning Research into Results: Characterizing the Dog Meat Trade in Bali to Help End the Trade Maria Maliga Vernandes Sasadara; Ni Putu VT Timur; I Ketut Puja; Nyoman S Dharmawan; Jennifer Hood
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The dog meat trade (DMT) in Bali is a black-market trade that threatens international tourism on both animal welfare and human health grounds. Outlets selling dog meat often advertise this by displaying ‘RW’ signs as this abbreviation stands for ‘rintek wuuk’ (soft fur) in Manadonese. The DMT is notoriously cruel and uses inhumane methods to catch, transport, and slaughter dogs, many of which are believed to be stolen.Dog meat is not a food under Indonesian law and as such is not subject to food safety or zoonotic disease regulation. In particular, the DMT is believed to contribute to the transmission of rabies, which is endemic in Bali.Despite this, there was little factual information about the DMT in Bali, and this was hindering Government efforts to close the trade. We believe our study is the first to characterize the DMT in Bali and provides insights as to how and why the trade exists, and what actions are needed to end it.
Prevalence of Skin Disorders in Kintamani Dog Ni Putu Vidia Tiara Timur; Putu Ayu Sisyawati Putriningsih; I Ketut Puja
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.654 KB)

Abstract

The aim of this study was to determine the prevalence of skin disorders in Kintamani Bali dog from 2009 to2013 using an observational study with purposive sampling technique. The data was taken from the patientrecords in veterinary clinics in Badung Regency, Gianyar Regency and Denpasar City which thereafteranalyzed descriptively. From 475 dogs, 99 dogs (20.8%) were infected by skin disorder. Skin disordersobserved in this study was caused by parasites (15.2%), fungus (2.1%), virus (2.1%) and bacteria (1.5%).The case of skin disorders frequently occurred in the dry season (53.1%) than in rainy season (46.9%), witha variation in age from 3 months to 12 years old.
Sensitifitas dan Spesifisitas Teknik Imunohistokimia Rabies I Ketut Wirata; I Ketut Berata; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 1 (2014)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.653 KB)

Abstract

Fluorescent Antibody Test (FAT) is a golden standard for rabies diagnosis, but it requires many sample preconditions before testing. Immunohistochemical technique (IHC) has the same principle as the FAT, however, it is more simple. This study was conducted to examine the sensitivity and specificity of IHC compare to FAT. The sensitivity and specificity were calculated using the formula of 2 x 2 contingency table and statistical analysis of the chi-square. Out of 40 samples that rabies positive on FAT, 27 samples (67.5%) showed rabies positive on IHC and 13 samples (32.5%) showed rabies negative on IHC. All samples that rabies negative on FAT were also rabies negative on IHC. The IHC has the sensitivity and specificity level 67.5% and 100% respectively. The IHC has a lower sensitivity than FAT, however, IHC has many advantages such as it can be performed on formalin fixed-samples. A further research is necessary to improve the sensitivity of IHC on detecting the rabies.
A Survey on Cases of Canine Transmissible Venereal Tumor (TVT) in Kintamani Dog Claudia Murti Andari; I Made Kardena; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

The cases of canine transmissible venereal tumor (TVT) in Kintamani Bali Dog were studied by evaluating the cases in clinical records from some veterinary clinics in the two regencies of Badung, Gianyar and Denpasar City. A total of 486 clinical records on Kintamani Bali Dogs gathered from 2010 to 2014 was used in this study. Among those, the specific cases on TVT diagnostic were observed. The study showed that the prevalence of TVT was catagorised low. Three of Kintamani Bali Dogs were diagnosed  to have TVT (0.62%). Canine transmissible venereal tumor affected both male and female dogs. The tumor cases were recorded in varied ages,  from 2 to 5 years. This result indicates that TVT in Kintamani Bali Dogs is likely to be associated with the dog’s sexual maturity.
Seroprevalensi Leptospirosis pada Anjing Kintamani di Bali . Mutawadiah; I Ketut Puja; Nyoman Sadra Dharmawan
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

The aim of this study was to determine the seroprevalence of leptospirosis in Kintamani dogs in Bali. Bloodsamples were collected from 55 Kintamani dogs. The sera examination was conducted in the VeterinaryResearch Centre, Bogor. Microscopic Agglutination Test was performed to detect the presence ofantibodies against 14 leptospira antigens. The results showed that the prevalence of leptospirosis inKintamani dogs in Bali was 18.18% (10/55). Two out of 10 positive sera samples (3.64%) reacted withthree serovar; 5 (9.09%) reacted with two serovar; and 3 (3.64%) reacted with one serovar. There were fourtypes of serovars detected in this study, namely: Leptospira interrorgans serovar Celledoni, Leptospirainterrorgans serovar Canicola, Leptospira interrorgans serovar Cynopteri dan Leptospira interrorgansserovar Ichterohemorrhagiae. The research found that Leptospira interrorgans serovar Cellodini was verycommon in Kintamani dog.
Penampilan Reproduksi Sapi Bali pada Peternakan Intensif di Instalasi Pembibitan Pulukan Mahmud Siswanto; Ni Wayan Patmawati; Ni Nyoman Trinayani; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Abstract

This studi was conducted to investigate the reproductive performances  of Bali cattle under intensive management in breeding instalation Pulukan Bali from  2008  to  2010.  Totaling 298 2 - 6.5 year old heifers were use to represents the benchmark reproductive performances of Bali cattle. Data collected were age at first heat (AFH), age at first calving (AFC), calving interval (CI), and services per conception (SPC). The overall means for  AFH, AFC,and  CI  were  718.57 ± 12.65; 1104.51 ±  23.82, and 350.46 ± 27.98 days  respectively,  and SPC was 1.65 ± 0.87.
Asosiasi Polimorfisme Mikrosatelit DRBP1 Gen BoLa (Bovine Leucocyte Antigen) dengan Ukuran Tubuh pada Sapi Bali Ni Wayan Patmawati; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Genomic selection has a potency for improving the genetic quality of livestock. In effort to find out a molecular marker or DNA segment which is linked with a certain phenotype, we examined the association of the polymorphism of microsatellite DRBP1 with the body size of Bali cattle. A total of 55 blood samples of sires was collected from the Breeding Center of Bali Cattle Improvement Office in District of Jembrana. Total DNA was extracted with QIAamp DNA blood mini kit. The locus was amplified using PCR technique and alleles were separated through PAGE 6%, and visualized with silver staining. We found 5 alleles which their length varied from 108 bp to 136 bp. Allele 122 had the highest frequencies (25.45%). The expected heterozygositiy (HE) and observed heterizygosities (HE), and the PIC were 0.783, 0.891 and 0.738 consecutively. Statistic analysis showed that the polymorphism of microsatellite DRBP1 had no significant association (p > 0.05) with body size of male Bali cattle. This indicates that the DRBP1 does not play a role in determining the body size.
Risk Factors and Spatial Distribution of Rabies in Bali in the Year of 2014 IGNA Wisnu Adi Saputra; I Nyoman Dibia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

The aims of this study was to determine the spatial distribution and the risk factors associated with theincidence of rabies in Bali on the year of 2014. A total of 1286 brain samples from all over the Bali wasexamined.  Out of  all brain samples, 130 samples were diagnosed positive for rabies. Based on these data,we collected the dogs location coordinates using a tool of Global Positioning System (GPS). Thosecoordinates  were, then, analyzed with GIS software. The results showed that spatial distribution of theincidence of rabies in each regencies in Bali varied, however, it had a same pattern. The factors ofcounseling, breed, trade, and geographical condition associated to the incidence of rabies. Further researchis needed to map the incidence of rabies over a longer time (more than 3 years), to improve theeffectiveness of surveillance in connection with the eradication of rabies in Bali.
Co-Authors . Mutawadiah Anak Agung Gde Oka Dharmayuda Claudia Murti Andari Deborah Bianti Purwanto Desak Nyoman Dewi Indira Laksmi Evayana, Made Fania, Bella Glantiga, I Gede Jaya Rama Gunawan, I Wayan Nico Fajar I Gede Hendi Saputra I Gusti Agung Arta Putra I Gusti Ngurah Bagus Trilaksana I Ketut Berata I Ketut Suardita I Ketut Suatha I Ketut Wirata I Made Dwinata I Made Kardena I Made Sukada I Made Yoga Windu Pradana i Nengah Wandia I Nyoman Dibia I Nyoman Sulabda I Wayan Bebas Ida Bagus Made Oka IGNA Wisnu Adi Saputra IVAN M TARIGAN Jennifer Hood Kadek Ari Sindawati Lontoh, Rudy Luh Made Sudimartini Made Raysa Merliana Mahmud Siswanto Mahmud Siswanto Manuela, Ni Luh Maria Maliga Vernandes Sasadara Mufa, Romy Muhammad Dary Ni Komang Sri Puspaningsih Ni Made Devityasih Perayadhista Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Werdi Susari Ni Putu Vidia Tiara Timur Ni Putu VT Timur Ni Wayan Patmawati Ni Wayan Patmawati Ni Wayan Patmawati Nyoman S Dharmawan Nyoman Sadra Dharmawan Putu Ayu Sisyawati Putriningsih Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Suastika Rudy Lontoh Siswanto - Siswanto Suastika , Putu Sukernayasa, I Wayan Tessa Yuli Oktaviami Tjok Gde Oka Pemayun TJOKORDA ISTRI AGUNG Cintya Dalem Trilaksana, IGN Yesy Febnica Dewi ZAKIATUN MUHAMMAD