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All Journal Hemera Zoa Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Kedokteran Hewan
I Ketut Puja
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Karakterisasi Genetik Kambing Gembrong dari Karangasem Bali Menggunakan DNA Mikrosatelit I Ketut Puja; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2632

Abstract

The present study was undertaken with primary objective to characterize of Gembrong goat breeds. It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. DNA Genome was isolated from hairs. Nine microsatellite were amflified by PCR. PCR product were run on 6% bis-Acrylamide gel in automated DNA sequencer. Flourescent signals from the dye-labeled microsatellite were detecting using STRand software. The result show that all markers (INRA005, INRA063, INRA023, ILSTS19, ILSTS87, SRCRSP8, MAF65, OarFCB20, and McM547) successfully amplified in Gembrong goat microsatellite loci. The number of alleles per locus ranged from 1 (INRA005) to 4 (ILSTS87). All the microsatellites investigated were found to be highly polymorphic, except INRA063. In total, 23 alleles were observed for the 9 microsatellite loci. The allele sizes ranged from 99 bp (OarFCB20) to 240bp (SRCRSP8). The study can be extended to include large many microsatellites in different chromosome location to validate the results.
Peluang Penggunaan Spermatozoa Epididimis Yang Dikoleksi Setelah Kematian Sebagai Sumber Sel Gamet pada Anjing I Ketut Puja; IGN Trilaksana; Rudy Lontoh
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2846

Abstract

The purpose of this study was to determine the effect of postmortem time on percentage of life epididymal sperm from postmortem canine caudae epididymides. A total of 9 dog were used and divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6 hours postmortem. This way, samples were obtained at different times postmortem. Sperm were extracted from the caudae epididymis by means of cuts. The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33± 5.60 (T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life was affected by postmortem time. There was significant decrease life sperm recovered from epididymides postmortem (P<0.01). In conclusion, epididymal sperm from dog undergo decrease of percentage of life, but it could stay acceptable within many hours postmortem. We intepreted these data to indicate that it may still be possible to obtain viable spermatozoa many hours later.
Penampilan Reproduksi Sapi Bali yang Dipelihara di Sentra Pembibitan Sapi Bali Sobangan I Gede Hendi Saputra; I Gusti Ngurah Bagus Trilaksana; I Ketut Puja
Buletin Veteriner Udayana Vol. 14 No. 4 August 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.775 KB) | DOI: 10.24843/bulvet.2022.v14.i04.p05

Abstract

This study was conducted to investigate the reproductive performance of bali cattle in breeding instalation bali cattle of Sobangan, Badung, Bali. Totaling 288 heifers were use to represents the benchmark reproductive performance of bali cattle. Data collected were service per conception (S/C), gestation length, birth weight, and postpartum estrus. The overall means for service per conception (S/C), gestation length, birth weight, and postpartum estrus were 1,3 times; 284,63±10,73 kg; 17,89±1,04 kg; dan 101,71±32,31 days.
HUBUNGAN UKURAN TUBUH TERHADAP PERILAKU ANJING KINTAMANI Siswanto Siswanto; Nyoman Sadra Dharmawan; I Ketut Puja; I Gusti Agung Arta Putra
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p19

Abstract

The Balinese believe that body size (the ratio of body length to snout length) in dogs is related to behavior, therefore body size is used to predict dog character. How to predict by measuring body size is a local wisdom that is still trusted for choosing a good dog. This belief has been running for a long time from their ancestors and has become a culture. Dog behavior according to local wisdom in Bali in general and in Sukawana Village, Kintamani District, Bangli Regency in particular, is categorized as: guna, jaya, forced, tek and kyul. Guna is a dog with an obedient character, useful and suitable as a hunting dog. Jaya is a dog with good character, attractive, energetic, tends to be hyperactive, this type of dog is also good for hunting. Force is a dog that is difficult to manage, requiring special handling, suitable for keeping the house. Tek is a dog with dirty behavior and difficult to train or manage and Kyul is a dog with a lazy character. A total of 66 Kintamani dogs were used as experimental animals. The research design used a cross-sectional study design. Body size observation data was then compared with clinical observations in the same dog. The results show that there is a relationship between behavior and body size (ratio of body length to snout length). It was concluded that body size (body length to snout length ratio) can be implemented to predict behavior.
ASOSIASI POLIMORFISME GENETIKA LOKUS DEOXYNUCLEIC ACID (DNA) MIKROSATELIT GEN BOVINE LYMPHOCYTE ANTIGEN (BoLA) DENGAN KUALITAS SEMEN PADA SAPI BALI I Ketut Puja; I Nengah Wandia; Putu Suastika; I Nyoman Sulabda
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.094 KB) | DOI: 10.21157/j.ked.hewan.v7i2.932

Abstract

Tujuan penelitian ini adalah mendapatkan informasi dasar mengenai struktur genetika menggunakan marka melokuler DNA mikrosatelit gen bovine lymphocyte antigen (BoLA) dan hubungannya dengan kualitas semen sapi bali. Data diambil dari 8 ekor sapi jantan yang ada di Unit Pelaksana Teknis Daerah (UPTD) Inseminasi Buatan, Baturiti, Tabanan, Bali. Hasil pemeriksaan kualitas sperma menunjukkan bahwa volume semen sapi bali 3,0-6,5 ml, konsentrasi spermatozoa 800-11.000 juta sel/ml, dan persentase spermatozoa motil 70-75%. Hasil penelitian pada sapi bali menunjukkan bahwa ketiga lokus mikrosatelit yang digunakan teramplifikasi pada sapi bali dan jumlah alel pada lokus RM185 dan BM1815 adalah 2 sedangkan jumlah alel pada lokus DRB3 adalah 1. Heterozigositas per lokus berkisar 0-0,533. Nilai PIC per lokus antara 0-0,375. Dari hasil penelitian ini dapat disimpulkan bahwa ukuran alel pada ketiga mikrosatelit berpotensi sebagai marka molekuler yang berperan terhadap kualitas semen pada sapi bali.
Identification and Prevalence of Aspergillus sp Isolated from Bali Dog’s Skin Sudipa, Putu Henrywaesa; Puja, I Ketut; Dharmayuda, Anak Agung Gde Oka; Gunawan, I Wayan Nico Fajar; Sudimartini, Luh Made; Jayanti, Putu Devi; Sukernayasa, I Wayan; Mufa, Romy Muhammad Dary
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21594

Abstract

Aspergillus sp are fungi that commonly caused a systemic respiratory infection in Human and Dog. In Bali, most of the dog are free-roaming dogs, and has poor skin health condition. Some of them are suspected with fungal infection. Aspergillus sp has high risk to infect the dog, because they are available everywhere in environment. Therefore, this research is conducted for adding primary data of Aspergillus sp isolated from dog’s skin and their prevalence. The samples are 15 free-roaming Bali dogs with suspected fungal infection. Identification was carried out by using microscopic and macroscopic observation methods, skin swab was taken from the dog’s skin using the Mackenzie method, and the sterile toothbrush swab gently from the dog’s skin to the Sabouraud Dextrose Agar (SDA) media. The fungi were cultured on SDA media for macroscopic identification for approximately 3-4 days, mostly to observe how the fungal colony grew and its color. Microscopic identification are using adhesive tape, the tape was gently pressed into the fungal colony, the tape was stuck into the surface of object-glass with 3-4 drops of Methylene Blue staining, and the object glass was examined by using a light microscope, the findings of Aspergillus sp fungi are recorded and the data are descriptively presented. The result shows that most Aspergillus species that were found is Aspergillus fumigatus and Aspergillus niger according to their colonies growth and microscopic appearance, and the prevalence from 15 samples were 53.33%. Keywords: Aspergillus sp, fungi, Bali Dogs, Identification, Prevalence
ASOSIASI POLIMORFISME GENETIKA LOKUS DEOXYNUCLEIC ACID (DNA) MIKROSATELIT GEN BOVINE LYMPHOCYTE ANTIGEN (BoLA) DENGAN KUALITAS SEMEN PADA SAPI BALI Puja, I Ketut; Wandia, I Nengah; Suastika, Putu; Sulabda, I Nyoman
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.932

Abstract

Tujuan penelitian ini adalah mendapatkan informasi dasar mengenai struktur genetika menggunakan marka melokuler DNA mikrosatelit gen bovine lymphocyte antigen (BoLA) dan hubungannya dengan kualitas semen sapi bali. Data diambil dari 8 ekor sapi jantan yang ada di Unit Pelaksana Teknis Daerah (UPTD) Inseminasi Buatan, Baturiti, Tabanan, Bali. Hasil pemeriksaan kualitas sperma menunjukkan bahwa volume semen sapi bali 3,0-6,5 ml, konsentrasi spermatozoa 800-11.000 juta sel/ml, dan persentase spermatozoa motil 70-75%. Hasil penelitian pada sapi bali menunjukkan bahwa ketiga lokus mikrosatelit yang digunakan teramplifikasi pada sapi bali dan jumlah alel pada lokus RM185 dan BM1815 adalah 2 sedangkan jumlah alel pada lokus DRB3 adalah 1. Heterozigositas per lokus berkisar 0-0,533. Nilai PIC per lokus antara 0-0,375. Dari hasil penelitian ini dapat disimpulkan bahwa ukuran alel pada ketiga mikrosatelit berpotensi sebagai marka molekuler yang berperan terhadap kualitas semen pada sapi bali.
Co-Authors . Mutawadiah Anak Agung Gde Oka Dharmayuda Claudia Murti Andari Deborah Bianti Purwanto Desak Nyoman Dewi Indira Laksmi Evayana, Made Fania, Bella Glantiga, I Gede Jaya Rama Gunawan, I Wayan Nico Fajar I Gede Hendi Saputra I Gusti Agung Arta Putra I Gusti Ngurah Bagus Trilaksana I Ketut Berata I Ketut Suardita I Ketut Suatha I Ketut Wirata I Made Dwinata I Made Kardena I Made Sukada I Made Yoga Windu Pradana i Nengah Wandia I Nyoman Dibia I Nyoman Sulabda I Wayan Bebas Ida Bagus Made Oka IGNA Wisnu Adi Saputra IVAN M TARIGAN Jennifer Hood Kadek Ari Sindawati Lontoh, Rudy Luh Made Sudimartini Made Raysa Merliana Mahmud Siswanto Mahmud Siswanto Manuela, Ni Luh Maria Maliga Vernandes Sasadara Mufa, Romy Muhammad Dary Ni Komang Sri Puspaningsih Ni Made Devityasih Perayadhista Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Werdi Susari Ni Putu Vidia Tiara Timur Ni Putu VT Timur Ni Wayan Patmawati Ni Wayan Patmawati Ni Wayan Patmawati Nyoman S Dharmawan Nyoman Sadra Dharmawan Putu Ayu Sisyawati Putriningsih Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Suastika Rudy Lontoh Siswanto - Siswanto Suastika , Putu Sukernayasa, I Wayan Tessa Yuli Oktaviami Tjok Gde Oka Pemayun TJOKORDA ISTRI AGUNG Cintya Dalem Trilaksana, IGN Yesy Febnica Dewi ZAKIATUN MUHAMMAD