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Phenotypic evaluation of gamma-induced dwarf mutants in Dendrobium crumenatum at M2 generation DWIYANI, RINDANG; DARMAWATI, IDA AYU PUTRI; KAWURI, RETNO; FITRIANI, YUYUN; KAMUHI, RINI HERMINA; HANIFAH, WAFA’ NUR; SANTIKA, I PUTU BENNY
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100150

Abstract

Abstract. Dwiyani R, Darmawati IAP, Kawuri R, Fitriani Y, Kamuhi RH, Hanifah WN, Santika IPB. 2026. Phenotypic evaluation of gamma-induced dwarf mutants in Dendrobium crumenatum at M2 generation. Asian J Agric 10 (1): g100150. https://doi.org/10.13057/asianjagric/g100150. Bali, Indonesia, has strong potential for the development of ornamental orchids, particularly compact cultivars with high commercial value. Dendrobium crumenatum, a native Indonesian orchid, is widely appreciated for its white, fragrant flowers; however, its tall growth habit limits its suitability as a potted ornamental plant. This study aimed to induce and evaluate dwarf phenotypes in D. crumenatum using gamma irradiation. Plantlets were exposed to 0, 5, 10, 15, and 20 Gy, arranged in a completely randomized design with five replicates and three plantlets per replicate (n=75). Morphological traits were assessed after in vitro culture and acclimatization. Data were analyzed using analysis of variance followed by the Least Significant Difference (LSD) test at a 5% significance level (p<0.05). Gamma irradiation significantly affected all measured traits. The 5 Gy treatment caused severe growth suppression, reducing plant height, leaf number, and shoot number by approximately 53.7%, 81.7%, and 84.4%, respectively, compared to the control. In contrast, the 10 Gy treatment achieved a similar reduction in plant height (~52.6%) while maintaining higher leaf number (~83.3% of control) and shoot number (~62.2% of control), indicating a more balanced morphological response. The 15 Gy treatment showed minimal deviation from the control, whereas 20 Gy produced semi-compact plants with moderate shoot retention. These results demonstrate a non-linear dose-response relationship, where intermediate doses (particularly 10 Gy) optimize the trade-off between dwarfism and vegetative performance. These findings indicate that medium gamma irradiation doses are effective for generating dwarf D. crumenatum mutants suitable for potted ornamental use and demonstrate the potential of mutation breeding to support orchid improvement and the floriculture industry.
A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture Dwiyani, Rindang; Fitriani, Yuyun; Ana, Francis Geren Sta; Bimantara, Putu Oki
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 2 (2024): October
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i2.85776

Abstract

Acclimatization is the final stage of propagation by in vitro culture. This phase is crucial in micropropagation, as it will determine the survival of plantlets outside culture jars. This phase’s efforts should ensure optimal conditions, including the media type. This study sought the best media for acclimating strawberry plantlets produced under meristem culture. The research employed the randomized block design, utilizing 7 various media treatments, which include husk charcoal; perlite; cocopeat; perlite + husk charcoal; soil + sand; husk charcoal + cocopeat; and perlite + cocopeat. The media mix was set at 1:1 a ratio (weight/weight), with 9 replicates. The chemical properties of the media were then analyzed, including several parameters such as organic C, total N, and water content. Several parameters were observed for plantlet’s growth parameters, including plant survival rates, number of leaves per plant, average leaf area, and root fresh weight. The results found that cocopeat was the best medium for acclimating strawberry plantlets. In cocopeat media, the plant survival rate reached 96.68%, with the leaves number of 7.67 plant-1, an average leaf area of 120.92 cm2 plant-1, and root fresh weight of 4.30 g plant-1. These results indicate that cocopeat is a medium derived from coconut fiber powder, a natural resource that can be renewed sustainably and produces better plant plantlet growth.
Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH Ana, Francis Geren Sta ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Bimantara, Putu Oki Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO ENDANG SEMIARTI Endang Semiarti Endang Semiarti ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti Hanifah, Wafa’ Nur HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti IDA AYU PUTRI DARMAWATI Ida Ayu Putri Darmawati Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KAMUHI, RINI HERMINA KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada Listihani, Listihani LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Pradana, I Kadek Wira Purba, Reza Stefany Purwantoro, A - Putu Suwardike Redana, I Made Basma Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu SANTIKA, I PUTU BENNY Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani