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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi Jurnal BALIRESO ad-Dawaa : Journal of Pharmaceutical Sciences Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Dikmas: Jurnal Pendidikan Masyarakat dan Pengabdian Pharmaceutical Reports Celebes Journal of Community Services Journal of Pharmaceuticals and Natural Sciences MPSJ
Asriani Suhaenah
Fakultas Farmasi, Universitas Muslim Indonesia, Makassar
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Hydroquinone Levels in Whitening Body Lotion Circulating in Makassar City by the UV-Vis Spectrophotometry Method Rezky M, Mega; Suhaenah, Asriani; Baits, Muzakkir
Pharmaceutical Reports Vol 2, No 2 (2023): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v2i2.272

Abstract

Hydroquinone is an active compound that is able to control pigment production to inhibit melanin formation and has activity as a skin whitening agent, so hydroquinone is widely used in cosmetic products. Hydroquinone has adverse side effects if used for a long time. BPOM RI has issued a regulation on the prohibition on the use of hydroquinone as a bleach in cosmetics. The purpose of this study was to identify the hydroquinone content in whitening handbody samples circulating in Makassar City and to find out the level of hydroquinone contained in the sample. Qualitative analysis is carried out using FeCl₃ reagents and quantitative analysis using the UV-VIS spectrophotometry method. The number of samples used in this study was as many as 4 samples that did not have a registration number from BPOM RI. The results showed that the 4 samples studied positively contained hydroquinone. The results of the sample test measured at a maximum wavelength of 292 nm, obtained hydroquinone levels at the sample code HB1 = 1.267%; sample code HB2 = 0.912%; sample code HB3 = 0.379% and sample code HB4 = 0.449%.
Formulation of Seaweed (Eucheuma cottonii Doty) Lotion Hamsinah, Hamsinah; Suhaenah, Asriani; Mulyana, Imam Dwiyan
Pharmaceutical Reports Vol 2, No 2 (2023): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v2i2.274

Abstract

Seaweed (Eucheuma cottonii Doty) has potential as an antioxidant because it contains kappa-carrageenan which can counteract free radicals. Formulation of seaweed (Eucheuma cottonii Doty) into lotion preparations can minimize the negative effects of sun exposure, because it can reflect solar radiation or work as a physical sunscreen. To give the maximum effect, particle size reduction is carried out, so that the smaller the particle size of the seaweed powder, the surface area increases. This study aims to determine the formulation and evaluate the preparation of lotion from seaweed powder. This research was conducted using an experimental method. The lotion was made with three variations of seaweed powder concentration (Eucheuma cottonii Doty), namely 2.5%, 3% and 5%. Tests were carried out before and after accelerated conditions at 5oC and 35oC for 12 hours each for 12 cycles with test parameters including organoleptic, homogeneity, pH, spreadability, viscosity and flow type. The results obtained in the organoleptic test showed no change in the three formulas both before and after the accelerated condition. The pH test showed that Formula 2 and Formula 3 still showed that the preparation remained in the skin pH range for sunscreen. In the homogeneity test, all preparations were homogeneous, as well as the spreadability test met the test requirements criteria. Based on the tests carried out, it can be concluded that formulas 2 and 3 have optimum stability.
Analysis of Artificial Sweeteners, Sodium Cyclamate, in Packaged Beverages and Powders by the Gravimetric Method Pratama, Shinta Indah; Suhaenah, Asriani; Tahir, Masdiana
Pharmaceutical Reports Vol 2, No 2 (2023): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v2i2.267

Abstract

Most people like instant beverage products because of their practical processing and superior taste and creative packaging, thus attracting consumers to consume them. One of the food additives that is often used in instant beverage products is artificial sweeteners such as sodium cyclamate which has a sweetness level of 3.94 kcal/g or 30 times that of sucrose. This study aims to determine the levels of sodium cyclamate in packaged and powdered drinks circulating on the campus of the Indonesian Muslim University using the gravimetric method. The sampling method used a purposive technique and obtained 6 test samples, qualitative analysis used the precipitation method and obtained all positive samples containing sodium cyclamate. The results of the quantitative analysis obtained the levels of sodium cyclamate for each sample A; B; C; D; E; F as much as 37.07 mg/kg; 30.17 mg/kg; 12.93 mg/kg; 4.31 mg/kg; 340 mg/kg; 258 mg/kg. Thus, these results indicate that all samples do not exceed the levels set by BPOM regulation No. 11 of 2019 concerning food additives, namely 350 mg/kg, so they are safe for consumption.
Determination of Phenolic and Flavonoid Content of Ethanol Extract of Manila Sawo Leaf (Manilkara zapota L.) from Wajo District by Uv-Vis Spectrophotometry Suhaenah, Asriani; Hartina, Suki; Aminah, Aminah
Pharmaceutical Reports Vol 3, No 2 (2024): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v3i2.311

Abstract

Sapodilla Manila (Manilkara zapota L.) is a plant that is widely known by the public. People usually use sapodilla leaves as traditional medicine. Efficacy obtained from the presence of phenolic compounds and flavonoids in these plants. This study aims to determine the phenolic and flavonoid content of ethanol extract in sapodilla leaves (Manilkara zapota L.) from Wajo district. This research method used UV-Visible spectrophotometry and the reference standard used were gallic acid (phenolic) and quarcetin (flavonoid). The results showed that the average phenolic content of the ethanol extract of sapodilla leaves at a concentration of 100 ppm was 150.28 mgGAE/g extract and the average flavonoid content of the ethanol extract of sapodilla leaves at a concentration of 300 ppm was 36.577 mgQE/g extract.
Aktivitas Antiinflamasi Ekstrak Etanol Dan Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) Dengan Menggunakan Metode Spektrofometri UV-Vis Suhaenah, Asriani; Nuryanti, Siska; Abidin, Zainal; Evatiara, Nur
Jurnal Fitofarmaka Indonesia Vol 11, No 2 (2024): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v11i2.1288

Abstract

Ficus is a species that is rich in polyphenolic compounds, such as flavonoids. These compounds are strong antioxidants that can aid in the prevention and treatment of various diseases caused by oxidative stress. They also protect cell structures, have anti-inflammatory properties, and can act as antibiotics. The aim of this study was to evaluate the anti-inflammatory properties of the ethanol extract and ethyl acetate fraction of rubber kebo (Ficus elastica) leaves using the Bovine Serum Albumin (BSA) protein denaturation method. The measurements were taken on a Uv-Vis spectrophotometer at a wavelength of 660 nm.  The study consisted of four experimental groups: a negative control, a positive control of diclofenac sodium, and test groups of ethanol extract and ethyl acetate fraction. The study revealed that the positive control (IC50) had an anti-inflammatory value of 9.2 μg/mL. Additionally, the ethanol extracts and ethyl acetate fractions of rubber kebo leaves (Ficus elastica) demonstrated anti-inflammatory activity with IC50 values of 31.24 μg/mL and 37.89 μg/mL, respectively. These findings suggest that the ethanol extracts and ethyl acetate fractions of rubber kebo leaves possess anti-inflammatory properties.
ANALISIS KANDUNGAN SENYAWA FENOLIK DAN TANIN PADA ISOLA FUNGI ENDOFIT (IFEBK20) BUNGA KERSEN (Muntingia calabura L) DENGAN METODE SPEKTROFOTOMETRI UV-VIS irmawan jamal p; St. Maryam; Asriani Suhaenah
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 2 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

Bunga Kersen (Muntingia calabura L.) mengandung fungi endofit yang mampu menghasilkan metabolit sekunder yang sama dengan sel inangnya, dimana metabolit sekunder ini dari isolat fungi endofit IFEBK20 seperti senyawa fenol dan tanin yang merupakan kelompok senyawa fenolik terbesar. Pada penelitian ini bertujuan untuk mengetahui berapa kadar senyawa fenolik dan tanin yang dihasilkan dari isolat fungi endofit bunga kersen (Muntingia calabura L.) secara kuantitatif menggunakan metode spektrofotometri UV-Vis. Dari hasil penelitian menunjukkan bahwa ekstrak isolat IFEBK20 bunga kersen (Muntingia calabura L.) mengandung senyawa fenol dan tanin. Secara kuantitatif ekstrak isolat IFEBK20 bunga kersen (Muntingia calabura L.) diukur pada panjang gelombang maksimum 739,901 nm untuk fenol dengan baku pembanding asam galat, dan diukur pada panjang gelombang maksimum 738,578 nm untuk tanin dengan baku pembanding asam tanat. Hasil penelitian menunjukkan bahwa kadar rata-rata ekstrak isolate IFEBK20 bunga kersen (Muntingia calabura L.) mengandung senyawa fenol sebesar 21,447 mgGAE/g ekstrak atau 2,144 % b/b GAE, dan senyawa tanin sebesar 31,584 mgTAE/g ekstrak atau 3,158 %
UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAUN KARET KEBO (Ficus elastica) DENGAN MENGGUNAKAN METODE FRAP Amita Hasyim; Asriani Suhaenah; Masdiana Tahir
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 3 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

Free radicals are atoms, molecules or compounds that can stand alone, having one or more unpaired electrons in their outermost orbitals. Antioxidants are molecules that are able to deactivate or stabilize free radicals. The kebo rubber leaf plant is known to have benefits as an antioxidant because it contains ingredients such as glycosides, flavonoids, phenolic acids, alkaloids, steroids, saponins, coumarins, tannins, and triterpenoids. The aim of this research was to test the antioxidant activity of the ethyl acetate fraction of kebo rubber leaves (Ficus elatica) using the FRAP (Ferric Reducing Antioxidant Power) method. The rubber kebo leaf extraction method uses the maceration method with 96% ethanol solvent. The ethanol extract was fractionated using the liquid-liquid partition method to obtain the ethyl acetate fraction. the sample fraction was added with several FRAP reagents and then measured using a UV-Vis spectrophotometer at a maximum wavelength of 712 nm with quercetin as a comparison. The results showed that the ethyl acetate fraction of kebo rubber leaves for antioxidant activity was 26.692 mgQE/g fraction.
Analisis Kandungan Rhodamin B Pada saus Tomat Yang Beredar DiKabupaten Wajo Kecamatan Belawa Menggunakan Metode Spektrofotometri UV-Vis Andi Isti Hudayanti; Harti Widiastuti; Asriani Suhaenah
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 2 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

Rhodamin B is a synthetic dye that is used as a dye for the textile industry which is dangerous. Rhodamin B is often misused as a food and cosmetic coloring agent, where Rhodamin B can cause irritation to the digestive tract, poisoning and can even cause cancer. This study aims to determine the presence or absence of Rhodamin B content in tomato sauce samples circulating in Wajo District, Belawa District. In this study used six samples marked with S1, S2, S3, S4, S5, and S6. Qualitative analysis used the TLC test and quantitatively for positive samples used the Uv-Vis spectrophotometry method. From the research results, it was found that S1, S2, S3, S4, S5 were negative for Rhodamin B while S6 was positive for Rhodamin B where the Rf value was 0.68, the color of the pink stain matched the Rhodamin B comparison.
ANTIOXIDANT ACTIVITY TEST OF THE ETHANOL EXTRACT OF AVOCADO SEED (Persea americana Mill.) USING THE FRAP METHOD Indah Kartika Ohorela; St. Maryam; Asriani Suhaenah
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

Avocado seeds (Persea americana Mill.) are a plant that is useful as a traditional medicine. The ethanol extract of avocado seeds contains several secondary metabolite compounds, namely alkaloids, triterpenoids, tannins, flavonoids and saponins. The aim of this research was to determine the antioxidant activity contained in avocado seed extract (Persea americana Mill.). using the FRAP method which was measured with a UV-Vis spectrophotometer at a wavelength of 794 nm. Extraction of avocado seeds (Persea americana Mill.) using the maceration method using 96% ethanol solvent. The results show that the antioxidant activity of the ethanol extract of Avocado Seeds is 42.448 mgQE/g of extract, which means that every 1 gram of ethanol extract of avocado seeds (Persea americana Mill.) is equivalent to 42,488 mg of quercetin.
Analisis aktivitas perlindungan sinar UV ekstrak etanol daun kakao (Theobroma cacao L.) berdasarkan nilai Sun Protection Factor. Julis Tina Rustam; St. Maryam; Asriani Suhaenah
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

UV rays have a bad impact on the skin, but they can be minimized by using ingredients that are UV protective. Sunscreen can be obtained from natural ingredients, one of which is Cocoa Leaves (Theobroma cacao L.) which contains flavonoid and phenolic compounds. The aim of this research was to determine the UV protection activity of ethanol extract of cocoa leaves (Theobroma cacao L.) originating from the Malino, Jeneponto and Wajo areas based on the Sun Protection Factor value. Measurement of the Sun Protection Factor (SPF) value using the UV-Vis Spectrometry method was measured at wavelengths of 290, 295, 300, 305, 310, 315 and 320 nm. The results showed that the ethanol extract of cocoa leaves (Theobroma cacao L.) had UV protection activity for the Malino area at a concentration of 0.01% of 3.25 (minimum protection), a concentration of 0.03% of 10.34 (maximum protection). , 0.05% concentration of 17.10 (ultra protection). For the Jeneponto area, the 0.01% concentration is 3.88 (minimum protection), the 0.03% concentration is 12.3 (maximum protection), the 0.05% concentration is 20.18 (ultra protection) and the Wajo area has a value at a concentration of 0.01% it is 3.38 (minimum protection), a concentration of 0.03% is 10.77 (maximum protection), a concentration of 0.05% is 17.05 (ultra protection). Ethanol extract of cocoa leaves (Theobroma cacao L.) originating from Jeneponto has the greatest UV protection activity with a value of 20.18 with ultra protection ability.
Co-Authors A. Muflihunna A.Hesti Wulandasari Amir Abd. Malik Abdul Malik Ahmad, Akstar Roskiana Ahmad, Aktsar Roskiana Aminah Aminah Aminah Aminah Aminah Aminah Amir, A. Hesti Wulandasari Amita Hasyim Andi Isti Hudayanti Arru'nnisa Ika Nur Ilmi Aris aulia azahra renhoran Bayu Putra, Bayu Dewi Yuliana Dita Wulan Bahari Evatiara, Nur Fadlina Chany Saputri Fitriani Asri Gusmiati Gusmiati Hamsinah Hamsinah Harti Widiastuti Harti Widiastuti Hartina, Suki Hasrianti, Hasrianti Hexatrianto Fatul Rahman Inayanti Fatwa Indah Ahmad Yani Indah Kartika Ohorela irmawan jamal p Izzatul Fikri Mujtahid Julis Tina Rustam Kamase, Jeni Mamat Pratama Mamat Pratama, Mamat Masdiana Tahir Masdiana Tahir Masdiana Tahir, Masdiana Mochammad Imron Awalludin Muammar Fawwaz Muhammad Ivan Sandyna Mulyana, Imam Dwiyan Muslimin Arafat Muzakkir Baits Muzakkir Baits Muzakkir Baits, Muzakkir Nasra Nasra Normawati Ismail nur evatiara Nurmaya Effendi Nurmaya Effendy nurrizky afiah arifin Nurul Fadhillah Amrullah Nurul Faiza Mahmud Nurul Ilmi Ainun Nissa Pratama, Shinta Indah Rachmat Faisal Syamsu Rais Razak, Rais Rezky M, Mega Rini Andriani Riski Mentari Jahri Rizqi Nur Azizah Rusniyanti, Rusniyanti Safitri, Ineks Saripa Indira Syahraini Latuconsina Siska Siska Nuryanti Siska Nuryanti, Siska St. Maryam St. Maryam Maryam St. Maryam, St. Tadjuddin Naid Virsa Handayani Yulinda Rahim Zainal Abidin Zainal Abidin zhafira anggraini mondo