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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi Jurnal BALIRESO ad-Dawaa : Journal of Pharmaceutical Sciences Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Dikmas: Jurnal Pendidikan Masyarakat dan Pengabdian Pharmaceutical Reports JURNAL PENGABDIAN MASYARAKAT YAMASI Celebes Journal of Community Services Journal of Pharmaceuticals and Natural Sciences MPSJ Jurnal Balireso: Jurnal Pengabdian pada Masyarakat
Asriani Suhaenah
Fakultas Farmasi, Universitas Muslim Indonesia, Makassar
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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EFEK ANTI INFLAMASI IN VITRO EKSTRAK ETANOL DAUN KARET KEBO (Ficus elastica) DENGAN METODE STABILISASI MEMBRAN Red Blood Cell (RBCs) Suhaenah, Asriani; Widiastuti, Harti; Aprianti, Tiara
As-Syifaa Jurnal Farmasi Vol 17, No 1 (2025): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v17i1.1299

Abstract

The rubber kebo plant (Ficus elastica) is traditionally known to have various benefits, including as an anti-inflammatory agent. This study aimed to evaluate the anti-inflammatory effect of ethanol extract of rubber kebo leaves using the red blood cell (RBCs) membrane stabilisation method in vitro. Kebo rubber leaves were extracted by a maceration method using 96% ethanol, and anti-inflammatory activity was tested by measuring the stability of RBCs membrane against hypotonic solution-induced haemolysis. The results showed that ethanol extract of rubber kebo leaves had anti-inflammatory activity with a percentage of membrane stabilisation of 31.156% at a concentration of 40 ppm and reached 51.998% at a concentration of 200 ppm. This activity is caused by the content of secondary metabolites such as flavonoids and tannins that work by stabilising the red blood cell membrane. Compared to the positive control of diclofenate sodium, the extract showed competitive effectiveness, albeit lower
ANALISIS KANDUNGAN SENYAWA FENOLIK DAN TANIN PADA ISOLA FUNGI ENDOFIT (IFEBK20) BUNGA KERSEN (Muntingia calabura L) DENGAN METODE SPEKTROFOTOMETRI UV-VIS p, irmawan jamal; Maryam, St.; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 2 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i2.33

Abstract

Bunga Kersen (Muntingia calabura L.) mengandung fungi endofit yang mampu menghasilkan metabolit sekunder yang sama dengan sel inangnya, dimana metabolit sekunder ini dari isolat fungi endofit IFEBK20 seperti senyawa fenol dan tanin yang merupakan kelompok senyawa fenolik terbesar. Pada penelitian ini bertujuan untuk mengetahui berapa kadar senyawa fenolik dan tanin yang dihasilkan dari isolat fungi endofit bunga kersen (Muntingia calabura L.) secara kuantitatif menggunakan metode spektrofotometri UV-Vis. Dari hasil penelitian menunjukkan bahwa ekstrak isolat IFEBK20 bunga kersen (Muntingia calabura L.) mengandung senyawa fenol dan tanin. Secara kuantitatif ekstrak isolat IFEBK20 bunga kersen (Muntingia calabura L.) diukur pada panjang gelombang maksimum 739,901 nm untuk fenol dengan baku pembanding asam galat, dan diukur pada panjang gelombang maksimum 738,578 nm untuk tanin dengan baku pembanding asam tanat. Hasil penelitian menunjukkan bahwa kadar rata-rata ekstrak isolate IFEBK20 bunga kersen (Muntingia calabura L.) mengandung senyawa fenol sebesar 21,447 mgGAE/g ekstrak atau 2,144 % b/b GAE, dan senyawa tanin sebesar 31,584 mgTAE/g ekstrak atau 3,158 %
UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAUN KARET KEBO (Ficus elastica) DENGAN MENGGUNAKAN METODE FRAP Hasyim, Amita; Suhaenah, Asriani; Tahir, Masdiana
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 3 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i3.76

Abstract

Free radicals are atoms, molecules or compounds that can stand alone, having one or more unpaired electrons in their outermost orbitals. Antioxidants are molecules that are able to deactivate or stabilize free radicals. The kebo rubber leaf plant is known to have benefits as an antioxidant because it contains ingredients such as glycosides, flavonoids, phenolic acids, alkaloids, steroids, saponins, coumarins, tannins, and triterpenoids. The aim of this research was to test the antioxidant activity of the ethyl acetate fraction of kebo rubber leaves (Ficus elatica) using the FRAP (Ferric Reducing Antioxidant Power) method. The rubber kebo leaf extraction method uses the maceration method with 96% ethanol solvent. The ethanol extract was fractionated using the liquid-liquid partition method to obtain the ethyl acetate fraction. the sample fraction was added with several FRAP reagents and then measured using a UV-Vis spectrophotometer at a maximum wavelength of 712 nm with quercetin as a comparison. The results showed that the ethyl acetate fraction of kebo rubber leaves for antioxidant activity was 26.692 mgQE/g fraction.
Analisis Kandungan Rhodamin B Pada saus Tomat Yang Beredar DiKabupaten Wajo Kecamatan Belawa Menggunakan Metode Spektrofotometri UV-Vis Hudayanti, Andi Isti; Widiastuti, Harti; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 2 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i2.104

Abstract

Rhodamin B is a synthetic dye that is used as a dye for the textile industry which is dangerous. Rhodamin B is often misused as a food and cosmetic coloring agent, where Rhodamin B can cause irritation to the digestive tract, poisoning and can even cause cancer. This study aims to determine the presence or absence of Rhodamin B content in tomato sauce samples circulating in Wajo District, Belawa District. In this study used six samples marked with S1, S2, S3, S4, S5, and S6. Qualitative analysis used the TLC test and quantitatively for positive samples used the Uv-Vis spectrophotometry method. From the research results, it was found that S1, S2, S3, S4, S5 were negative for Rhodamin B while S6 was positive for Rhodamin B where the Rf value was 0.68, the color of the pink stain matched the Rhodamin B comparison.
ANTIOXIDANT ACTIVITY TEST OF THE ETHANOL EXTRACT OF AVOCADO SEED (Persea americana Mill.) USING THE FRAP METHOD Ohorela, Indah Kartika; Maryam, St.; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.221

Abstract

Avocado seeds (Persea americana Mill.) are a plant that is useful as a traditional medicine. The ethanol extract of avocado seeds contains several secondary metabolite compounds, namely alkaloids, triterpenoids, tannins, flavonoids and saponins. The aim of this research was to determine the antioxidant activity contained in avocado seed extract (Persea americana Mill.). using the FRAP method which was measured with a UV-Vis spectrophotometer at a wavelength of 794 nm. Extraction of avocado seeds (Persea americana Mill.) using the maceration method using 96% ethanol solvent. The results show that the antioxidant activity of the ethanol extract of Avocado Seeds is 42.448 mgQE/g of extract, which means that every 1 gram of ethanol extract of avocado seeds (Persea americana Mill.) is equivalent to 42,488 mg of quercetin.
Analisis aktivitas perlindungan sinar UV ekstrak etanol daun kakao (Theobroma cacao L.) berdasarkan nilai Sun Protection Factor. Rustam, Julis Tina; Maryam, St.; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.243

Abstract

UV rays have a bad impact on the skin, but they can be minimized by using ingredients that are UV protective. Sunscreen can be obtained from natural ingredients, one of which is Cocoa Leaves (Theobroma cacao L.) which contains flavonoid and phenolic compounds. The aim of this research was to determine the UV protection activity of ethanol extract of cocoa leaves (Theobroma cacao L.) originating from the Malino, Jeneponto and Wajo areas based on the Sun Protection Factor value. Measurement of the Sun Protection Factor (SPF) value using the UV-Vis Spectrometry method was measured at wavelengths of 290, 295, 300, 305, 310, 315 and 320 nm. The results showed that the ethanol extract of cocoa leaves (Theobroma cacao L.) had UV protection activity for the Malino area at a concentration of 0.01% of 3.25 (minimum protection), a concentration of 0.03% of 10.34 (maximum protection). , 0.05% concentration of 17.10 (ultra protection). For the Jeneponto area, the 0.01% concentration is 3.88 (minimum protection), the 0.03% concentration is 12.3 (maximum protection), the 0.05% concentration is 20.18 (ultra protection) and the Wajo area has a value at a concentration of 0.01% it is 3.38 (minimum protection), a concentration of 0.03% is 10.77 (maximum protection), a concentration of 0.05% is 17.05 (ultra protection). Ethanol extract of cocoa leaves (Theobroma cacao L.) originating from Jeneponto has the greatest UV protection activity with a value of 20.18 with ultra protection ability.
ANALISIS KADAR SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN KAKAO (Theobroma cacao L.) DENGAN PERBANDINGAN DAERAH TEMPAT TUMBUH Mahmud, Nurul Faiza; Maryam, St; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.246

Abstract

Cocoa leaves (Theobroma cacao L.) contain active compounds that have many health benefits. Flavonoids are one of the active compounds contained therein that can prevent the formation of free radicals and reduce tissue damage due to inflammation. This study aims to determine the flavonoid content of ethanol extract of cacao leaves (Theobroma cacao L.) based on the comparison of growing regions using the UV-Vis spectophotometric method. Samples were taken from three regions namely Jeneponto, Wajo and Malino. This research method begins with the preparation of samples by maceration with 96% ethanol solvent, then qualitative analysis is carried out by Thin Layer Chromatography (KLT) method and quantitative analysis is carried out measuring quercetin standards and determining flavonoid levels with a UV-Vis spectrophotometer at a maximum wavelength of 430 nm. The results showed that the ethanol extract of cocoa leaves (Theobroma cacao L.) positively contained flavonoids and had flavonoid levels in the Jeneponto, Wajo, Malino regions of 45.812 mgQE/g extract; 41.556 mgQE/g extract, and; 38.343 mgQE/g extract, respectively. Where the ethanol extract of cocoa leaves (Theobroma cacao L.) from the Jeneponto region has higher flavonoid levels than the other two regions.
Perbandingan Kadar Perbandingan Kadar Fenolik Ekstrak Daun Karet Kebo (Ficus elastica) Hasil Ekstraksi Bertingkat Menggunakan Metode Spektrofotometri UV-Vis mondo, zhafira anggraini; Suhaenah, Asriani; Tahir, Masdiana
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.278

Abstract

ZHAFIRA ANGGRAINI MONDO. Comparison of Phenolic Content of Rubber Kebo (Ficus elastica) Leaf Extract Results from Multilevel Extraction Using the UV-Vis Spectrophotometry Method (Supervised by Asriani Suhaenah and Masdiana Tahir). The kebo rubber plant (Ficus elastica) is used by Indonesian people as traditional medicine, namely medicine to lower blood pressure, lower cholesterol, stroke, and reduce joint pain. Kebo rubber leaves (Ficus elastica) contain secondary metabolites such as saponins, polyphenols, flavonoids and tannins. This research aims to determine the comparison of phenolic compound levels of kebo rubber leaf extract (Ficus elastica) resulting from multilevel extraction using the UV-vis spectrophotometric method. The extraction process uses 3 different types of solvents, namely n-hexane (nonpolar), ethyl acetate (semipolar) and ethanol (polar) using a sonicator. Determination of phenolic content using the UV-Vis spectrophotometric method, with a maximum wavelength of 772 nm and using gallic acid as a comparison. The results showed that the levels were influenced by the solvent used in comparing the phenolic compound levels of kebo rubber leaf extract (Ficus elastica) with the multilevel extraction method, namely n-hexane extract 3,774 mgGAE/g, ethyl acetate extract 12,696 mgGAE/g, ethanol extract 74,396 mgGAE/g g.
PENENTUAN KADAR TANIN PENENTUAN KADAR TANIN PADA EKSTRAK ETANOL DAUN KARET KEBO (Ficus elastica Roxb. ex Hornem) DENGAN MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-Vis renhoran, aulia azahra; Suhaenah, Asriani; Muflihunna, A.
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.281

Abstract

This research aims to determine the tannin content in the ethanol extract of kebo rubber leaves (Ficus elastica Roxb. ex Hornem). Rubber plants (Ficus elastica Roxb. ex Hornem) are plants that are widely used as traditional medicine, the leaves of this plant are used as medicine to treat diarrhea. , stops bleeding and treats hemorrhoids. Many of the benefits and properties of the kebo rubber plant (Ficus elastica Roxb. ex Hornem) cannot be separated from the compounds contained in it, such as phenolics, tannins, flavonoids and saponins. Simplicia was extracted using the maceration method with 96% concentration of ethanol solvent for 3x24 hours. Determination of tannin content using the UV-Vis spectrophotometric method, with a maximum wavelength of 772 nm, using gallic acid as a comparison. The research results showed that the total tannin content in the ethanol extract was an average of 31.708mgQE/g.
Comparasion Of Levels Perbandingan Kadar Flavonoid Ekstrak Daun Karet Kebo (Ficus elastica)Hasil Ekstraksi Bertingkat Menggunaka evatiara, nur; Suhaenah, Asriani; Tahir, Masdiana
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.282

Abstract

NUR EVATIARA. Comparison of flavonoid levels of rubber plant leaf extract (Ficus elastica) resulting from multilevel extraction using the UV-Vis spectrophotometric method. (Supervised by Asriani Suhaenah and Masdiana Tahir). Rubber plant (Ficus elastica) is a plant in the Moraceae family whose leaves have been empirically stated to contain secondary metabolite compounds such as saponins, polyphenols, flavonoids and tannins which have been used as medicine for coughing up blood, improving urine flow, relieving pain and improving blood circulation. The aim of this research was to determine the comparison of levels of flavonoid compounds in rubber plant leaf extract (Ficus elastica) resulting from multilevel extraction using the UV-Vis spectrophotometric method. The multistage extraction process uses 3 different types of solvents, namely n-hexane (nonpolar), ethyl acetate (semipolar), and ethanol (polar) using a sonicator. Determination of flavonoid levels using the UV-Vis spectrophotometric method, with a maximum wavelength of 432 nm and quarcetin as a comparison. The research results were influenced by the solvent used in comparing the levels of flavonoid compounds, namely rubber plant leaf extract (Ficus elastica) with a multilevel extraction method, namely N-hexane extract 7.161 mgQE/g, Ethyl acetate extract 7.663 mgQE/g, 96% Ethanol extract 10.592 mgQE/g g.
Co-Authors A. Muflihunna, A. A.Hesti Wulandasari Amir Abd. Malik Abdul Malik Ahmad, Akstar Roskiana Ahmad, Aktsar Roskiana Aminah Aminah Aminah Aminah Amir, A. Hesti Wulandasari Aprianti, Tiara arifin, nurrizky afiah Aris, Arru'nnisa Ika Nur Ilmi Asri, Fitriani Bahari, Dita Wulan Bayu Putra, Bayu Damang, Irda Binti Dewi Yuliana Evatiara, Nur Fadlina Chany Saputri Gusmiati Gusmiati Hamsinah Hamsinah Harti Widiastuti Harti Widiastuti Hartina, Suki Hasrianti, Hasrianti Hasyim, Amita Hexatrianto Fatul Rahman Hudayanti, Andi Isti Inayanti Fatwa Ismail, Normawati Kamase, Jeni Mahmud, Nurul Faiza Mamat Pratama Mamat Pratama, Mamat Masdiana Tahir mondo, zhafira anggraini Muammar Fawwaz Mujtahid, Izzatul Fikri Mulyana, Imam Dwiyan Muslimin Arafat Muzakkir Baits Muzakkir Baits, Muzakkir Nasra Nasra Nurlina Nurmaya Effendy Nurul Fadhillah Amrullah Nurul Ilmi Ainun Nissa Nurwijayanti Ohorela, Indah Kartika p, irmawan jamal Pratama, Shinta Indah Rachmat Faisal Syamsu Rais Razak, Rais renhoran, aulia azahra Rezky M, Mega Rini Andriani Riski Mentari Jahri Rizqi Nur Azizah Rusniyanti, Rusniyanti Rustam, Julis Tina Safitri, Ineks Sandyna, Muhammad Ivan Saripa Indira Syahraini Latuconsina Saskia, Eka Putri Siska Siska Nuryanti Siska Nuryanti, Siska St Maryam, St St. Maryam St. Maryam Maryam St. Maryam, St. Tadjuddin Naid Virsa Handayani Yani, Indah Ahmad Yulinda Rahim Zainal Abidin Zainal Abidin