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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Berkala Perikanan Terubuk JBIO: jurnal biosains (the journal of biosciences) Biogenesis: Jurnal Ilmiah Biologi Jurnal Biologi Universitas Andalas Jurnal Biologi Tropis Best Journal (Biology Education, Sains and Technology) Indonesian Journal of Laboratory Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat
Dewi Imelda Roesma
Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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In-silico Design and Testing of Tetra-Primer Arms-PCR for SNPs-Based Identification of Sumatran Tiger (Panthera tigris sumatrae): ARMS-PCR SNP Identification of Sumatran Tiger Asrori, Ikrima; Novarino, Wilson; Tjong, Djong Hon; Roesma, Dewi Imelda
Journal of Tropical Life Science Vol. 16 No. 01 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.01.03

Abstract

The Sumatran tiger (Panthera tigris sumatrae) is a subspecies of tiger classified as critically endangered by the International Union for Conservation of Nature (IUCN) due to poaching and illegal trade. In the law enforcement process, it is very important to ensure that the sample is indeed from a Sumatran tiger. Seized samples from illegal trade cannot be identified based on their morphology because they have been degraded and processed into other forms. Previous studies have reported the use of molecular markers for Sumatran tiger identification, which involve lengthy procedures and analyses. Therefore, for a more effective identification process, faster and more accurate techniques are needed. This study aims to design tetra primers for the identification of Sumatran tiger subspecies, particularly from incomplete confiscated samples, using the Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) technique based on Single Nucleotide Polymorphism (SNP) markers. The primers were designed using the Primer1 application and validated in silico with CloneManager and NCBI BLAST, as well as through direct testing on Sumatran tiger blood samples. The design results showed one pair of primers with optimal specifications, producing G allele fragments (452 bp), A allele fragments (408 bp), and internal control fragments (819 bp). Specificity testing results indicated that all samples were Sumatran tigers, with amplification bands at 452 bp and 819 bp. These results demonstrate that the developed tetra-primer ARMS-PCR assay provides a rapid and practical molecular approach for identifying Sumatran tiger samples, particularly for forensic verification of confiscated wildlife materials.
Co-Authors - Syaifullah Aadrean Aadrean, Aadrean Agesi, Anugrah Viona Aida Fitriani Sitompul Aidil, Dyta Rabbani Annisya Fitri Anugrah Viona Agesi Arum Setiawan Asrori, Ikrima Aurora, Dhea Apriano Dahelmi Dahelmi Dahelmi Dian Juliadmi Djong Hon Tjong Dwinda K Fitri Dyta Rabbani Aidil Elida Hafni Siregar Fauzania, Salsa Huda, Nadyatul Khaira Indra Junaidi Zakaria Jabang Nurdin Janra, Muhammad Nazri Mairawita Mida Yulia Murni Mildawati Nofrita Nofrita Nurul Aida Prawira, Furqan Dwiki Lintang Salis, Viola Mutiara Sidik, Muhamad Rayhan Silvia Indra Solfiyeni Solfiyeni Syaifullah Syaifullah Syaifullah Syaifullah Syaifullah Taufik Rahman Tjong, Djong Usio, Nisikawa Vera Pujani Wilson Novarino Yuliwan Saputra Yusron Ritonga Zainal Zainal Zulkarnain, Alivia