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All Journal Journal of Food and Pharmaceutical Science Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Pharmaceutical Science and Technology Majalah Obat Tradisional Science and Technology Indonesia Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal Ilmu Kefarmasian Indonesia Pharmaceutical Sciences and Research (PSR) Jurnal Farmasi Sains dan Praktis Journal of Halal Science and Research Journal of Food and Pharmaceutical Sciences
Any Guntarti
Fakultas Farmasi Universitas Ahmad Dahlan, Yogyakarta
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Other

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Journal : Science and Technology Indonesia

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Analysis of Hydrolyzed Collagen in Facial Serum for Halal Authentication Using FTIR Spectroscopy and Multivariate Calibration Salamah, Nina; Firdaus, Rizqi Amalia; Guntarti, Any; Ahda, Mustofa; Uddin, Abm Helal; Kurniawan, Muhammad Fariez
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1270-1279

Abstract

Cosmetic trends in Indonesia are on the rise, particularly for facial serum products. However, most of these products lack halal certification, posing an important concern for Muslim consumers due to the potential use of haram substances, such as pork gelatin. This study seeks to identify the source of gelatin in serum products using the Fourier Transform Infrared (FTIR) spectroscopy and chemometric analysis. The samples used in this study include a reference facial serum formulated with pure bovine and porcine gelatin concentrations in the ratios of 1:0, 8:2, 5:5, 2:8, and 0:1, three hydrolyzed collagen serums that are not yet labeled halal and available on Shopee, and one serum that is labeled halal. The method used was FTIR combined with PLS chemometrics and PCA. The isolation of facial serum gelatin was performed using acetone at -20oC, followed by analysis via FTIR at wave numbers ranging from 4000 to 400 cm-1. FTIR results indicated the presence of functional groups in gelatin constituents, including C O, N H, C N, and C H aliphatic. The wavelengths employed for PLS and PCA analysis ranged from 1631 to 1430 cm-1. Calibration results showed R2=0.9936 and RMSEC=3.0445%. Internal validation yielded RMSECV=0.1674% and R2=0.9994 whilst external validation yielded RMSEP=0.9981% and R2=0.9910. Lastly, PCA analysis revealed that one halal-labeled serum sample contained bovine gelatin, whereas the three examined commercial serums were free from both pork and bovine gelatin.
Co-Authors Abdul Rohman Abidin, Muhamad Ali Zainal Abidin, Muhamad Ali Zainal Agsari, Aulia Dwi Agustinus Yuswanto, Agustinus Ajeng Dian Pertiwi, Ajeng Dian Aldri Frinaldi Alifiya, Fhahira Anwar, Shinta Desy Anwar, Shinta Desy Aprilia Kusbandari, Aprilia Ar Rahma, Nur Aulia Ardianti, Agustina Aristiani, Windy Atmadja, Dimas Azzahra, Fara Citra Ariani Edityaningrum, Citra Daud, Intan Dian Prasasti Efiana, Nuri Ari farida, fitri FATMAWATI, ARUM Firda Rahmadani, Firda Firdaus, Rizqi Amalia fitri farida Hari Susanti Hari Susanti Haryati, Husnul Hidayati, Sholihatil Hikmah, Nazila Nur Husna, Muhimmatul Ibnu Gholib Gandjar Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Ichwan Ridwan Rais Iin Narwanti Iis Wahyuningsih Irfan, Nawwar Islamy, Muhammad Rido Islamy, Muhammad Rido Jamhari Jamhari Jaya, Farida Kamal, Zainul Khairurrizki, Amanda Khayatulisma, Dika Kurniawan, Muhammad Fariez Laela Hayu Nurani Lalu Muhammad Irham Mahendra, Muhammad Reza Ma’ruf, Muhammad Milanie, Rida Dwi Moch Saiful Bachri Muhammad Ma'ruf Muhammad Yusron Maulana El-Yunusi Mukti Utomo, Adhitya Ilham Mustofa Ahda Narwanti, Iin Nina Salamah Nur Ismiyati Prativi, Seshilia Rarasati Putri, Hilma Putri, Hilma Rasyidah, Ilmiyatun Ria Maulida Rini Sulistyawati Rizki Amelia Rohmiati, Rohmiati Rohmiati, Rohmiati Ruliyani, Anita Rustandi, Tedi Sembiring, Rinawati Siti Rofida Sudibyo Martono Sugiyanto - Sulistyorini, Dwi Agustin Tri Octaviani Uddin, ABM Helal Wahyuningtyas, Nurma Warsi Warsi Warsi, Warsi Wati, Wahyu Irna Widea Rossi Desvita Widi Astuti, Silvia Ferry Wulandari, Rizkiyanti Yuningtyas, Ratna Zainab Zainab Zainab, Zainab