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Induksi pemijahan ikan wader pari (Rasbora argyrotaenia) menggunakan Ovaprim TM dengan dosis berbeda Dwi Retna Kumala Ningrum; Darmawan Setia Budi; Laksmi Sulmartiwi
Depik Vol 8, No 2 (2019): August 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.8.2.14076

Abstract. Silver rasbora (Rasbora argyrotaenia) has become one of the freshwater culture commodities that have high economic value. Research on the use of Ovaprim™ to the induction of spawning of silver rasbora needs to be done as an effort to develop the technology of fish hatchery. This study aims to determine the effect of using Ovaprim™ and its optimal dosage on the induction of silver rasbora spawning. This research was conducted at the Wet Laboratory Airlangga University Banyuwangi campus in March to May 2018. Ovaprim ™ was applicated using intramuscular injection method following the treatment design was used a Completely Randomized Design (CRD) with 4 treatments and 5 replications namely P0: Without Ovaprim ™ (control); P1: dose of 0,3 mL /kg fish weight; P2: dose of 0,5 mL/kg fish weight; P3: dose of 0,7 mL/kg weight of fish. The parameters observed in this study were fecundity, egg diameter, fertilization rate, hatching rate and larval survival rate. Induction of silver rasbora spawning using Ovaprim ™ has an effect on fecundity and fertilization rate but has no effect on hatching rate, survival rate and egg diameter. The optimal dose of Ovaprim ™ on the induction of spawning of silver rasbora is 0,7 mL/kg weight of fish with the highest fecundity value compared to other treatments.Keywords: fecundity, egg diameter, hatching rate, survival rate Abstrak. Ikan wader pari (Rasbora argyrotaenia) telah menjadi salah satu komoditas budidaya air tawar yang memiliki nilai ekonomis tinggi. Penelitian mengenai penggunaan Ovaprim™ dalam induksi pemijahan ikan wader pari perlu dilakukan sebagai upaya pengembangan teknologi pembenihan ikan tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan Ovaprim™ dan menentukan dosis optimalnya pada induksi pemijahan ikan wader pari. Penelitian ini dilakukan di Laboratorium Basah PSDKU Universitas Airlangga Banyuwangi pada bulan Maret sampai Mei 2018. Aplikasi Ovaprim™ menggunakan metode injeksi intramuskular mengikuti desain perlakuan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu P0 : Tanpa pemberian Ovaprim™ (kontrol), P1 : dosis 0,3 mL/kg bobot ikan, P2 : dosis 0,5 mL/kg bobot ikan, P3 : dosis 0,7 mL/kg bobot ikan. Parameter yang diamati dalam penelitian ini adalah fekunditas, diameter telur, derajat fertilisasi, derajat penetasan telur dan tingkat kelangsungan hidup larva. Induksi pemijahan ikan wader pari menggunakan Ovaprim™ memberikan pengaruh terhadap fekunditas dan derajat fertilisasi namun tidak memiliki pengaruh terhadap derajat penetasan telur, tingkat kelangsungan dan diameter telur. Dosis optimal Ovaprim™ pada induksi pemijahan ikan wader pari yaitu 0,7 mL/ kg bobot ikan dengan nilai fekunditas tertinggi dibanding perlakuan lainnya. Kata kunci: fekunditas, diameter telur, derajat penetasan telur, tingkat kelangsungan hidup

Induksi pemijahan ikan wader pari (Rasbora argyrotaenia) menggunakan Ovaprim TM dengan dosis berbeda Dwi Retna Kumala Ningrum; Darmawan Setia Budi; Laksmi Sulmartiwi
Depik Vol 8, No 2 (2019): August 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.8.2.14076

Abstract. Silver rasbora (Rasbora argyrotaenia) has become one of the freshwater culture commodities that have high economic value. Research on the use of Ovaprim™ to the induction of spawning of silver rasbora needs to be done as an effort to develop the technology of fish hatchery. This study aims to determine the effect of using Ovaprim™ and its optimal dosage on the induction of silver rasbora spawning. This research was conducted at the Wet Laboratory Airlangga University Banyuwangi campus in March to May 2018. Ovaprim ™ was applicated using intramuscular injection method following the treatment design was used a Completely Randomized Design (CRD) with 4 treatments and 5 replications namely P0: Without Ovaprim ™ (control); P1: dose of 0,3 mL /kg fish weight; P2: dose of 0,5 mL/kg fish weight; P3: dose of 0,7 mL/kg weight of fish. The parameters observed in this study were fecundity, egg diameter, fertilization rate, hatching rate and larval survival rate. Induction of silver rasbora spawning using Ovaprim ™ has an effect on fecundity and fertilization rate but has no effect on hatching rate, survival rate and egg diameter. The optimal dose of Ovaprim ™ on the induction of spawning of silver rasbora is 0,7 mL/kg weight of fish with the highest fecundity value compared to other treatments.Keywords: fecundity, egg diameter, hatching rate, survival rate Abstrak. Ikan wader pari (Rasbora argyrotaenia) telah menjadi salah satu komoditas budidaya air tawar yang memiliki nilai ekonomis tinggi. Penelitian mengenai penggunaan Ovaprim™ dalam induksi pemijahan ikan wader pari perlu dilakukan sebagai upaya pengembangan teknologi pembenihan ikan tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan Ovaprim™ dan menentukan dosis optimalnya pada induksi pemijahan ikan wader pari. Penelitian ini dilakukan di Laboratorium Basah PSDKU Universitas Airlangga Banyuwangi pada bulan Maret sampai Mei 2018. Aplikasi Ovaprim™ menggunakan metode injeksi intramuskular mengikuti desain perlakuan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu P0 : Tanpa pemberian Ovaprim™ (kontrol), P1 : dosis 0,3 mL/kg bobot ikan, P2 : dosis 0,5 mL/kg bobot ikan, P3 : dosis 0,7 mL/kg bobot ikan. Parameter yang diamati dalam penelitian ini adalah fekunditas, diameter telur, derajat fertilisasi, derajat penetasan telur dan tingkat kelangsungan hidup larva. Induksi pemijahan ikan wader pari menggunakan Ovaprim™ memberikan pengaruh terhadap fekunditas dan derajat fertilisasi namun tidak memiliki pengaruh terhadap derajat penetasan telur, tingkat kelangsungan dan diameter telur. Dosis optimal Ovaprim™ pada induksi pemijahan ikan wader pari yaitu 0,7 mL/ kg bobot ikan dengan nilai fekunditas tertinggi dibanding perlakuan lainnya. Kata kunci: fekunditas, diameter telur, derajat penetasan telur, tingkat kelangsungan hidup

Potensi Sedasi Minyak Atsiri Daun Bandotan (Ageratum conyzoides) terhadap Ikan Koi (Cyprinus carpio) [Sedation Potential Essential Oil of Bandotan Leaf (Ageratum conyzoides) to Koi Fish (Cyprinus carpio)] Laksmi Sulmartiwi; Boedi Setya Rahardja; Ade Wahyu Pratama
Jurnal Ilmiah Perikanan dan Kelautan Vol. 9 No. 2 (2017): Jurnal Ilmiah Perikanan dan Kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v9i2.7639

AbstrakTransportasi ikan berhubungan dengan metode pembiusan. Pembiusan ini dilakukan untuk menurunkan metabolisme atau keaktifan (sedative). Metabolisme yang tinggi selama transportasi dapat diminimalkan dengan menggunakan metode imotilisasi. Dengan suhu atau senyawa metabolik, bahan antimetabolik alami yang dapat digunakan adalah tanaman bandotan (Ageratum conyzoides). Bandotan merupakan tanaman obat yang mengandung minyak atsiri dan saponin (Kardono, 2003). Pada bidang perikanan, untuk mengurangi stres pada ikan dan juga digunakan menenangkan serta penurunan keaktifan (sedative) atau obat analgesik yang digunakan pada hewan vertebrata (Neiffer and Stamper, 2009). Tujuan dari penelitian ini untuk mengetahui potensi sedasi pemberian minyak atsiri daun bandotan terhadap ikan koi (Cyprinus carpio). Hasil analisis varian (ANAVA) menunjukkan bahwa setiap perlakuan minyak atsiri daun bandotan memberikan pengaruh terhadap waktu pingsan, waktu pulih sadar dan tachiventilasi (p<0,05). Rentang waktu pingsan tercepat pada perlakuan I saat menit ke- 27-30 dan rentang waktu pingsan terlama pada perlakuan D saat menit ke- 55. Rentang waktu pulih sadar tercepat pada perlakuan D saat menit ke- 5 dan rentang waktu pulih sadar terlama pada perlakuan I saat menit ke- 15-18. Rata-rata tachiventilasi tertinggi pada perlakuan A pada pengamatan menit ke- 60 (810 bit/5 menit) dan terendah pada I (343 bit/5 menit) pada pengamatan menit ke- 60. Berdasarkan pengukuran kualitas air yaitu suhu air antara 29-31o C, oksigen terlarut 6 ppm, pH berkisar 8-8,3 dan amoniak 0,02-0,27 mg/l. AbstractTransportation of fish associated with the method of anesthesia. Anesthesia is done to decrease metabolism or liveliness (sedative). A high metabolism during transport can be minimized by using imotilisasi. Imotilisasi can be done by using a low temperature or chemical or natural metabolic compounds (Soedibya and Pramono, 2006). Antimetabolic natural ingredients that can be used is a plant bandotan (Ageratum conyzoides). Bandotan is a medicinal plant that contains essential oils and saponins (Kardono, 2003). In the field of fisheries, to reduce stress on the fish and also used a calming as well as a decrease in the activity (sedative) or analgesic drugs used in vertebrate animals (Neiffer and Stamper, 2009). The purpose of this study to determine the potential for sedation administration bandotan leaf essential oil to the koi (Cyprinus carpio). Results of analysis of variance (ANOVA) showed that each treatment bandotan leaf essential oil to give effect to the time unconscious, conscious and tachiventilasi recovery time (p<0.05). Range fastest time in treatment I fainted while 27-30 minutes and the longest time span in treatment D fainted while 55 minute span of the fastest recovering conscious at the time of treatment D 5 minutes and recovered aware longest time span in treatment I while 15-18 minutes. The highest average tachiventilasi on treatment A on the 60 minute observation (810 bits / 5 min) and the lowest in the I (343 bits / 5 min) on minute 60 observation. Based on the measurement of water quality is the water temperature between 29-31o C, dissolved oxygen is 6 ppm, pH ranged from 8 to 8.3 and ammonia 0.02 to 0.27 mg / l

Analisis Secara Makroanatomi dan Mikroanatomi pada Gonad Ikan Puso (Harpadon nehereus) yang Ditangkap di Perairan Ujung Pangkah dan Perairan Weru, Jawa Timur [Macroscopical and Microscopical Analysis on the Gonad of Bombay Duck Fish (Harpadon nehereus) Caught in Ujung Pangkah and Weru Waters, East Java] Laksmi Sulmartiwi; Rr. Juni Triastuti; Merdeka Agus Saputra
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i2.11178

Abstrak Survei langsung yang dilakukan pada bulan September menunjukkan bahwa ikan puso merupakan salah satu ikan yang menjadi hasil tangkapan di Perairan Ujung Pangkah dan Perairan Weru. Larangan penangkapan ikan di breeding ground (daerah memijah) dan spawning ground (daerah bertelur) merupakan upaya untuk mencegah terjadinya penangkapan berlebihan (over exploitation). Berdasarkan hal tersebut perlu dilakukannya studi aspek reproduksi ikan puso secara makroanatomi dan mikroanatomi agar dapat dijadikan sebagai dasar untuk mengelola dan mengembangkan ikan puso, sehingga ikan tersebut dapat dipertahankan keberadaanya. Penelitian ini bertujuan untuk mengetahui aspek reproduksi dan tahap perkembangan gonad ikan puso secara makroanatomi dan mikroanatomi pada Perairan Ujung Pangkah dan Perairan Weru. Metode penelitian yang digunakan yaitu metode deskriptif, pada penelitian dibuat deskripsi tentang aspek reproduksi pada gonad ikan puso secara makroanatomi dan mikroanatomi. Berdasarkan hasil penelitian menunjukkan bahwa aspek reproduksi pada gonad ikan puso dari Perairan Ujung Pangkah dan Perairan Weru secara makroanatomi dan mikroanatomi meliputi tahap maturing terjadi pada pada kisaran panjang 28,6-31 cm, tahap mature terjadi pada kisaran panjang 26,1-27,2 cm dan tahap resting terjadi pada panjang 30,5 cm.Tahap perkembangan gonad ikan puso secara makroanatomi pada Perairan Ujung Pangkah dan Perairan Weru meliputi TKG I, TKG II menuju TKG III dan TKG III, sedangkan tahap perkembangan gonad ikan puso secara mikroanatomi pada Perairan Ujung Pangkah dan Perairan Weru meliputi tahap TKG II, TKG III dan TKG V. Abstract The direct surveillance conducted in month of September indicated that bombay duck fish is one of the fish catch yields in Ujung Pangkah and Weru Waters. The prohibition regulation of catching fish in breeding ground and spawning ground is the effort to prevent fish from over-exploitation. Therefore, it requires a research about reproduction aspects study of the bombay duck fish in macroscopical and microscopical way in order to obtain information stood for exploring and developing the bombay duck fish so as the fish can keep sustainable. This research is aimed to identify the reproduction aspects and the development stage of bombay duck fish gonad in macroscopical and microscopical way in Ujung Pangkah and Weru Waters. The research method applied was descriptive method by obtaining the data regarding reproduction aspects of bombay duck fish in macroscopical and microscopical way. As the result, the research showed that the reproduction aspects of bombay duck fish including maturing stage in the range of length between 28,6 cm, mature stage in the range of length between 26,1-27,2 cm and the resting stage occured 30,5 cm. The development of bombay duck fish gonad in macroscopical way in Ujung Pangkah and Weru Waters includes first maturity rate, second maturity rate to third maturity rate and third maturity rate. In contrast, The development of bombay duck fish gonad in microscopical way in Ujung Pangkah and Weru Waters includes second maturity rate, third maturity rate and fifth maturity rate.

Patologi Anatomi Ikan Komet (Carassius auratus auratus) Akibat Infestasi Argulus japonicus Jantan dan Betina Pada Derajat Infestasi yang Berbeda [Anatomic Pathology of Comet Fish (Carassius auratus auratus) As Result of Males and Females Argulus Japonicus Infestation in Different Degree ] Kismiyati Kismiyati; Laksmi Sulmartiwi; Selvi Oktora Mahanani
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i2.11181

Abstrak Usaha perikanan terutama ikan hias air tawar merupakan alternatif usaha untuk menjalankan perekonomian. Salah satu kendala dalam budidaya ikan komet adalah penyakit, terutama parasit. Infestasi ektoparasit Argulus japonicus dapat mengakibatkan kematian dan kerugian ekonomi bagi pembudidaya. Tujuan dari penelitian ini adalah untuk dapat mengetahui perubahan atau kelainan pada kulit ikan komet yang disebabkan oleh ektoparasit Argulus japonicus jantan dan betina. Penelitian ini dilaksanakan pada bulan Agustus-September 2015 di Laboratorium Pendidikan Fakultas Perikanan dan Kelautan Universitas Airlangga. Metode yang digunakan dalam penelitian ini adalah metode eksperimental. Pengaruh perlakuan terhadap perubahan patologi anatomi diolah dengan analisis statistik Analisis Variansi Rancangan Acak lengkap Pola Faktorial. Penelitian ini menggunakan dua kelompok perlakun yaitu kelompok satu menggunakan Argulus japonicus jantan dan kelompok dua menggunakan Argulus japonicus betina. Masing-masing kelompok menggunakan empat perlakuan dan lima ulangan. Variabel yang diamati dalam penelitian ini adalah perubahan patologi anatomi pada kulit ikan komet. Skoring dilakukan untuk menentukan derajat kerusakan patologi anatomi kulit ikan komet. Hasil penelitian ini menunjukkan bahwa infestasi ektoparasit Argulus japonicus jantan dan betina dapat mengakibatkan perubahan patologi anatomi pada ikan komet, tingkat kerusakan patologi anatomi ikan komet yang terjadi mengikuti derajat infestasi ektoparasit Argulus japonicus, perlakuan infestasi masing-masing Argulus japonicus jantan dan betina juga berpengaruh terhadap kerusakan patologi anatomi ikan komet, dan kondisi pemeliharaan ikan komet yang baik selama penelitian dapat meminimalkan pengaruh lain di luar pengaruh infestasi Argulus japonicus. Abstract Fisheries especially freshwater fish is an alternative attempt to run the economy. One of the obstacles in comet fish farming is a disease, especially parasites. Argulus japonicus ectoparasites infestations can be lead to death and economic loss to farmers. The purpose of this study was to determine the transformation or abnormalities in comet fish skin caused by ectoparasite Argulus japonicus male and female. This study was conducted in August-September 2015 in the Laboratory of Education Faculty of Fisheries and Marine Airlangga University. The method used in this study is the experimental method. Effect of treatment of pathological changes in anatomy processed with statistical analysis Variance Analysis Patterns Factorial Complete Randomized Block Design. This study used two groups of treatments namely the one using Argulus japonicus two groups of males and females using Argulus japonicus. Each group uses four treatments and five replications. The variables were observed in this study is the change in the anatomic pathology comet fish skin. Scoring is done to determine the degree of damage to fish skin anatomic pathology comet. The results showed that the infestation of ectoparasites Argulus japonicus males and females may lead to changes in anatomical pathology in fish comet, the extent of damage of anatomic pathology comet fish that occurs following the degree of infestation of ectoparasites Argulus japonicus, treatment of infestation Argulus japonicus both males and females that also affects the damage in anatomic pathology of comet fish, and the condition of pisciculture comet good throughout the study to minimize the influence of other outside influence Argulus japonicus infestations.

Perubahan Hematologi Ikan Mas Komet (Carassius auratus auratus) Akibat Infestasi Argulus japonicus Jantan Dan Argulus japonicus Betina [Hematologic Changes Comet Goldfish (Carassius auratus auratus) as a Result of Infestation Argulus japonicus Males And Argulus japonicus Females] Kismiyati Kismiyati; Laksmi Sulmartiwi; Norma Isnawati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11192

Abstract Diseases of the ornamental fish farming is mainly caused by ectoparasites. Ectoparasites are one cause of the declining value of the commodity selling ornamental fish in Indonesia. Argulus japonicus acute infestation levels could cause death and economic loss to farmers.The aim of this study was to determine the hematologic changes comet goldfish (Carassius auratus auratus) were infested A. japonicus males and females at different degrees of infestation. Research conducted at the Laboratory of Fisheries and Marine Laboratory of Airlangga University and the University of Airlangga Veterinary clinic and Center for Health Laboratory Surabaya in October 2014. The method used in this study is the experimental method. Data hemoglobin, hematocrit, erythrocytes, leukocytes were analyzed using ANOVA to determine the effect of treatment in the study group. This study used four treatments and five replicates with two groups. The variables were observed in this study were hematologic changes due to infestations of A. japonicus. Scoring is done to determine the hematologic changes comet goldfish.

Pengaruh Kedalaman Sarang Penetasan Penyu Hijau (Chelonia mydas) terhadap Masa Inkubasi dan Persentase Keberhasilan Penetasan di Pantai Sukamade, Taman Nasional Meru Betiri, Banyuwangi Jawa Timur [Effect of Nesting Site Depth of Green Turtle (Chelonia mydas) on the Incubation Period and Hatching Success Percentage in Sukamade Beach, Meru Betiri National Park, Banyuwangi in East Java] Laksmi Sulmartiwi; Wahju Tjahjaningsih; Abang Aldhian R. Putera
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11206

Abstract Indonesia is an archipelago that is rich in diversity of flora and fauna. One of these is a wealth of fauna species of sea turtles. All species of sea turtles in the world by the Convention on International Trade in Endangered Species of Wild Flora and Fauna (CITES) put in Appendix I as endangered and protected and not allowed to be traded. The purpose of this study was to determine effect the ratio of the depth from nest egg incubation and hatching percentage of green turtles (Chelonia mydas) nests at different depths in Sukamade, Betiri Meru National Park, Banyuwangi, East Java. The design of the study is a randomized block design (RGD). The groups as replicates for RGD, between groups or experimental material media are considered uniform. The results showed that the average incubation period was 58.87 +0.39 days, then the results were followed by Duncan's multiple range test, which showed that the highest yield obtained by the depth of 50 cm wasn't significantly different (p> 0.05) with depth of 30 cm and 70 cm. While the results obtained at the lowest depth of 90 cm was significantly different (p <0.05) with the other depth (30 cm, 50 cm and 70 cm). While the percentage of hatching success obtained in average hatching was 88.12 + 0.38%. The Duncan's multiple range test analysis, showed that at a depth of 70 cm were not significantly different (p <0.05) from depth of 50 cm, but 70 and 50 cm depth was significantly different (p> 0.05) from another depth. Whereas the lowest hatching results obtained on depth of 90 cm are significantly different (p> 0.05) from the other depth (30 cm, 50 cm and 70 cm). The conclusion of this study was different depth of green turtle nests affect the egg incubation period and the percentage of egg hatching success of green turtles.

Pengaruh Pemberian Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Dalam Transportasi Tertutup Benih Ikan Koi (Cyprinus Carpio) [The Effects Of Bandotan Leave's (Ageratum conyzoides) Essential Oil within Closed System Transportation of Koi Carp (Cyprinus carpio) ] Laksmi Sulmartiwi; Ida Bagus Putu Oka Dantika; Rr. Juni Triastuti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11311

Abstract In the ornamental fish business, transportation has an important role to distributing fish up into the costumers. The distance between the farm and the customers location, require a transportation method which can maintain the quality and quantity of ornamental fish during the transportation. The biggest challenge in live fish closed system transportation is to reduce the stress level of fish during the process. According to Harmon (2009), fish are often exposed to multiple stressor during the transportation. Stressed fish can be seen through the increasing of tachiventilation (rapid opercula movement) and blood glucose levels (Evans and Claiborne, 2006). One of the method to reduce stress of fish during transportation is using essential oils from Bandotan's (A. conyzoides) leaves. A. conyzoides essential oils know has an analgesic activity (Okunade, 2002; Shekhar and Anju, 2012; Singh et al., 2013). A chemical compound with known analgesic effect, used in human and veterinary medicine as local analgesic dan be used to fish for the purpose of sedation, immobilization, and analgesia as well as general anaesthesia (Zahl and Samuelsen, 2012). This research was conducted to see the effect of A.conyzoides essential oils toward the survival rate, blood glucose levels, and tachyventilation of koi carp juveniles during closed system transportation. This research using completely randomized design method which consist of 5 different treatments with 4 replications. Treatment that used in this research is the variation of administration doses of A. conyzoides essential oils during the koi carp juvenile closed system transportation. The treatment such as A (5 ppm), B (10 ppm), C (15 ppm), D (20 ppm), and E (0 ppm). Main parameter of this research is survival rate, blood glucose levels, and tachyventilation of koi carp juvenile during 8 hours transportation. The result shows that A. conyzoides essential oils give a significant result toward survival rate, blood glucose levels, and tachiventilation of koi carp juvenile during transportation. There is no mortality in treatment A, B, and E, during transportation. The lowest blood glucose levels and tachiventilation found in treatment A (5 ppm).

Bioaktivitas Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Terhadap Respon Fisiologis (Glukosa Darah Dan Tachiventilasi) Ikan Koi (Cyprinus carpio) [Bioactivity Essential Oil Of Bandotan Leaf (Ageratum conyzoides) On Koi (Cyprinus carpio) Physiological Responses (Blood Glucose And Tachiventilation) ] Laksmi Sulmartiwi; Mardiah Rahma Umami; Moch. Amin Alamsjah; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11388

Abstract Koi (C. carpio) is one of famous ornamental fish in Indonesia. Fish often be subject of change or stessor like physical, chemical and biology on culture. Stress in fish caused released cortisol hormone and cathecolamine hormone. Cathecolamine increase glicogenolisis, cardiovascular regulation and respiration fungtion. Blood glucose and tachiventilation (opercular movement) can indicate stress respon in fish. Essential oil of A. conyzoides have central analgesic activity. Analgesic drugs in vertebrae can reduce stress in fish. Essential oil shown inhibitory effect of GABA transminase, an enzime GABA (gammaaminobutryic acid) degradation which can provoking sedation. Sedation effect in fish reduce blood glucose and tachiventilation. Potency bioactive essential oil of bandotan leaf compound haven't been know in aquaculture. The potency based of LC50 (Lethal Consentration 50 %) value Purpose of this research determined effect of essential oil Ageratum conyzoides leaf on Koi (Cyprinus carpio) Phsycology Response (Blood Glucose and Tachiventilation) every 4 hours durung 24 hours and LC50-24 hours. Research was conducted at Laboratory of Faculty of Fisheries and Marine Airlangga University Surabaya. Research design used Completely Randomized Design and followed by Duncan's Multiple range test. Results of analysis of variance (ANAVA) from early observation and every 4 hours during 24 hours day showed that each treatment of essential oil Ageratum conyzoides leaf gave a significantly different effect on the blood glucose and tachiventilation (P <0.05). The highest average of blood glucose was found in D treatment at second observation (443,67 mg/dl) and the lowest was found in C treatment (60,67 mg/dl). The highest average of tachiventilation was found in D treatment at first observation (1730 bit/9 minute) and the lowest was found in A treatment (437,67 bit/9 minute) at early observation. LC50-24 hours essenial oil Ageratum conyzoides leaf is 34,047 ppm. Based on measurements of water quality, treatments was still viable and good to supported viability of Cyprinus carpio during research, water temperature range between 29-31oC, range of dissolved oxigen 4-6 mg/l, pH ranges 7,5-8,3 and 0,0060,27 mg/l on ammonia.

Distribusi Penyakit Infectious Myo Necrosis Virus (IMNV) Pada Udang Vannamei (Litopenaeus vannamei) Di Pantai Utara Jawa Timur [ Distribution Of Infectious Myo Necrosis Virus (IMNV) In White Shrimp Vannamei (Litopenaeus vannamei) In North Coast, East Java] Laksmi Sulmartiwi; Andre Rekasana; Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11424

Abstract White shrimp vannamei (Litopenaeus vannamei) is the main commodities among shrimp farmers because it has a rapid growth, can be cultivated with high density and world market demand is large enough .Since being introduced in Indonesia, vannamei shrimp has spread to the north of East Java pesisisr like Probolinggo, Pasuruan, Lamongan and Tuban. Shrimp growth factors are must be faced on vannamei shrimp culture, including the problems of disease. One type of virus disease is an Infectious Myo Necrosis Virus (IMNV). IMNV has affecting to the 70% of shrimp mortality (Tang et al., 2008). This study aims to determine the distribution of IMNV on white shrimp vannamei from the northern coast of East Java. This research uses descriptive method by taking samples from five different location which 75 samples totaly. In this research also take the water quality parameter including pH, DO, salinity and temperature. Whole of the samples performed the PCR test to determine how many samples which is indicating of IMNV. Based on measurements of water quality at five different locations, the plots I, II and III obtained the temperature range between 29.3-30.2 °C, salinity 90-30 ppt, pH 7.5-7.9, and DO 3.8-5.7 ppm. Temperature, pH and DO parameters show normally on white shrimp vannamei, while salinity shows different valve from normal conditions. Abnormallity happened in Situbondo with the salinity betwen 32-33 ppt and Pasuruan betwen 9-10 ppt. Results showed that the number of the lowest infection is Lamongan (53%) following Probolinggo (60%), Tuban (67%), Pasuruan (73%) and the highest is from Situbondo (100%)

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