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Effects of Different Acetic Acid Immersion Time on the Properties of Collagen from Pangasius Skin Clara Amelia Kusumawinahyu; Sharilla Aryananti Abidin; Patmawati; Dwi Yuli Pudjiastuti; Dwitha Nirmala; Mochammad Amin Alamsjah; Laksmi Sulmartiwi; Raseetha Vani Siva Manikam
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.33532

Abstract

Highlight Research Experimental use of pre-treated pangasius skin as collagen raw material using different acetic acid immersion time Effects of immersion time on the yield of collagen from pangasius skin Effects of immersion time on the proximate content of collagen from pangasius skin Presenting SDS-Page profile of collagen from pangasius skin   Abstract Pangasius sp. production in Indonesia has reached 384,310 tons in 2019. One of the main waste in pangasius fillet industry is the skin, which reached to 6% of body mass. Fish skins are alternative for making collagen because it has no restrictions for certain religions and ethnicities. Collagen is a protein biomaterial which acts as the main component of connective tissue. Extracting collagen using organic acids such as acetic acid is the most common extraction method. Herein, we report the effects of different immersion time of acetic acid to collagen properties from pangasius skin. In this study, pangasius skin was treated using 10% alcohol with the ratio 1:10 (w/v) for defatting and 0.1M NaOH with the ratio 1:10 (w/v) for eliminating non-collagenous protein. The immersion of 0.5M acetic acid was carried out on pangasius skin with the ratio of 1:20 (w/v), for 24, 48, and 72h at temperature less than 15oC. The sample was salted-out for 24 hours with NaCl until the concentration reached 2.5M, then the sample was freeze-dried at -40oC. This study investigated that longer immersion time affected the properties of pangasius skin collagen. Different immersion time significantly affected the yield of collagen from pangasius skin (p<0.05). Pangasius skin has a potential to be used as collagen raw material, and 72h of immersion time (P3) is suggested to produce pangasius skin collagen with the highest yield and protein content (6.15 % and 9.26 %). Production of collagen from the fish skin will contribute to increase pangasius waste valorization in pharmaceutical industry.
Effect of Acetic Acid Pretreatment on Hydro-extraction of Water-Soluble Collagen from Skin of Alaska Pollock (Theragra chalcogramma) Patmawati; Aliffiansyah Rizky Ergion; Laksmi Sulmartiwi; Raseetha Vani Siva Manikam; Dwitha Nirmala; Yaowapha Waiprib; Sapta Wijayanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.41274

Abstract

Highlight Research The optimal condition for production of water-soluble collagen from Alaska pollock fish skin was the pretreatment with 0.15 M acetic acid followed by hydro-extraction at 40oC with 150 rpm for two hours. Higher acetic acid concentration at a pretreatment step enhanced the higher yield of water-soluble collagen via hydro-extraction process. Hydro-extraction was an environmentally friendly method for converting Alaska pollock fish skin to water-soluble collagen. Alaska pollock fish skin could be used as an alternative source for collagen production. Abstract To date, there is no information on skin of Alaska Pollock (Theragra chalcogramma) being used as a source for collagen. In order to produce water-soluble collagen from the skin of Alaska Pollock, a process known as hydro-extraction is utilized. This technique does not need a long extraction time or a large amount of chemical reagent. The purpose of this study was to investigate the effect of acetic acid pretreatment on hydro-extraction of water-soluble collagen from Alaska Pollock skin. The skin samples were pretreated using acetic acid at different concentrations (0.01 M; 0.05 M; 0.1 M, 0.15 M) for two hours at chilling temperature. The pretreated skin samples were further processed to produce water-soluble collagen using the hydroxy-extraction method. The obtained collagen was analyzed for proximate compositions, yield, and amino acids compositions with high performance liquid chromatography. It was found that the proximate compositions of the collagen products, specifically the protein content (75%) and fat content (1%), met the requirements of the Indonesian National Standard (SNI 8076:2014). The collagen yield ranged from 2.6 to 3.13%. The predominate amino acids in collagen were glycine, arginine, proline, glutamic acid, serine, and alanine. Pretreatment of skin sample with 0.15 M acetic acid resulted in the highest yield of water-soluble collagen (3.13%) and protein content (91.13%). The skin of Alaska Pollock fish could be used as an alternative raw material to produce water-soluble collagen for medical, pharmacy or food processing applications.
Gambaran Histopatologi Kulit Ikan Kerapu Cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus) yang Terinfestasi Zeylanicobdella arugamensis Setelah Perendaman Menggunakan Perasan Daun Pepaya (Carica papaya) Bernathdo Mahendra Robbi Putra; Gunanti Mahasri; Laksmi Sulmartiwi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10575

Abstract

Increasing cultivation activities and intensifying ponds has the potential to give rise to problems, namely increasing cases of disease in grouper fish, namely Zeylanicobdella, causing mass deaths of up to 100%. This study aims to analyze the histopathological picture of grouper skin infested with Zeylanicobdella arugamensis after administering papaya leaf juice with different soaking times and concentrations of papaya leaf juice. The method used was experimental Completely Randomized Design (CRD) with a factorial pattern consisting of two factors, namely factor A, different concentrations of papaya leaf juice consisting of 0% (A1), 0.1% (A2), 0.3% (A3) , and 0.5% (A4), as well as factor B for grouper rearing time consisting of the 0th minute (B1), 60th minute (B2), and 120th minute (B3) with five repetitions. The results of the study showed that the soaking time of papaya leaf juice in grouper fish had an effect on reducing the degree of Zeylanicobdella infestation, there were changes in the histopathological picture and water quality values before and after soaking. Treatments A2, A3, and A4 experienced a decrease in the degree of ectoparasite infestation at 60 and 120 minutes of immersion. The histopathological scoring values for treatments A2, A3, and A4 decreased at 60 and 120 minutes of maintenance. The best treatment combination was 0, 3% and soaking time at 120 minutes. In conclusion, giving papaya leaf juice with a soaking time of 120 minutes with a concentration of 0.3% can reduce the degree of infestation and the histopathological scoring value of Zeylanicobdella in grouper fish.
Margarine as a Value-Added Product from Fish Oil, A By-Product of Lemuru Fish Canneries Citra Rachmania Wardhani; Mochammad Amin Alamsjah; Laksmi Sulmartiwi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10715

Abstract

Lemuru fish (Sardinella lemuru) is rich in omega-3 so it is widely used as raw material for fisheries production such as fish canning which produces by-products in the form of crude fish oil which needs to be purified by adding adsorbents so that it becomes pure fish oil. The adsorbent that can be used in the purification of fish oil is activated charcoal. Fish oil can be used as raw material for making added value products such as margarine. The aim of this study is to analyse the characteristics of margarine as a value-added product made from fish oil. Research shows that purification with activated charcoal produces fish oil with %FFA and PV that are in accordance with IFOS. Fish oil margarine in this study showed organoleptic value of colour 7.4 and aroma 7.07, emulsion stability 100%, %FFA content 0.28 ± 0.08, PV 1.6 ± 0.173, and higher 0.39% EPA and 2.58% DHA content compared to commercial margarine. In previous study showed margarin from sardine fish oil contains 19.5% EPA and 10.5% DHA which is also not found in commercial margarine from palm oil. The difference in EPA and DHA content between this study and previous study may be due to the fact that the fish oil in this study was obtained from by-products of canning processing with high temperatures which may cause a decrease in fatty acid levels due to protein denaturation.
Karakteristik Fisikokimia, Organoleptik, dan Kandungan Gizi Mayones Minyak Ikan Lemuru (Sardinella sp.) Nazhry Zahra Arifah; Mochammad Amin Alamsjah; Laksmi Sulmartiwi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10712

Abstract

Mayonnaise is a semi-solid emulsion processed product that has a very high fat content because the basic ingredients consist of vegetable oil (70-80%) and egg yolk (15-20%). Generally, mayonnaise has a high fat and calorie content. High fat content if consumed excessively will cause various diseases such as cholesterol, coronary heart disease and the risk of obesity. The use of lemuru fish oil as a substitute for mayonnaise is still rarely done. The protein content in lemuru fish is high enough that can be used as a source of protein. In addition to protein, lemuru fish oil is also enriched by the PUFA content of DHA of 30.10%. The aim of this research was to determine the quality of mayonnaise based on the organoleptic value and stability of the lemuru fish oil mayonnaise emulsion after a shelf life test. Proximate fat analysis using the Soxhlet method, protein analysis using the Kjeldahl method, ash analysis using a furnace, crude fiber analysis using the gravimetric method, water content analysis by evaporating the water in the material using an oven, carbohydrate analysis using the differential method. Shelf life test using the Accelerated Shelf Life Test method. Organoleptic testing uses a hedonic scale (1-5), with a total of 30 panelists. Emulsion stability test using a centrifuge by calculating the volume of oil separated. This study used a factorial randomized block design with two factors, namely temperature (5, 25, 35°C) and time (7, 14, 21 days). Mayonnaise proximate results from all parameters have met SNI except for fat and calorie parameters which tend to be lower than SNI standards. The ANOVA test results showed significant differences in organoleptic color and aroma, but it did not show significant differences in organoleptic texture and mold. The best organoleptic results at one-week storage. The emulsion stability results showed significant differences due to temperature and storage time factors.The best emulsion stability results at one week storage at 5, 25 and 35°C with a value of 100%.
Increased THC, DHC, PA and Survival of White Shrimp (Litopenaeus vannamei) by Feeding Porphyridium cruentum Nanoparticle Saniya Lailatul Qodriyah; Satyantini, Woro Hastuti; Nova Erika; Laksmi Sulmartiwi
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.67769

Abstract

A common disease in white shrimp is vibriosis which can cause acute hepatopancreatic necrosis or Acute Hepatopancreatic Necrosis Disease (AHPND). Immunostimulants are one of the alternatives to defend against pathogen infection. Porphyridium cruentum is a natural substance that can be used as an immunostimulant. The development of nanotechnology is progressing rapidly today due to its important role in various fields. The use of P. cruentum nanoparticles enhances the absorption and efficiency of feed, making their application more effective. The purpose of this study is to determine the effect of the addition of P.cruentum nanoparticles in feed on the enhancement of the immune response of white shrimp after administration for one month of rearing.  Shrimp were divided into four groups, one group was not fed with P.cruentum nanoparticles (K), three other groups were fed with P.cruentum nanoparticles 0.8; 1.0; 1.2% per kg of feed respectively. The results showed that the treatment with the addition of P.cruentum nanoparticles provided an increase in Total Hemocytes Count (THC), Differential Hemocyte Cells (DHC), and phagocytic activity (PA) after administration for one month of rearing and at the end of rearing provided a higher survival rate than the control.
Molecular Identification of Snapper (Perciformes: Lutjanidae) Landed at Pondokdadap Fishing Port of Sendang Biru, Malang, Indonesia Sapto Andriyono; Novian Aji Pradana; Laksmi Sulmartiwi; Andi Aliah Hidayani; Md. Jobaidul Alam; Adrian Damora; Ahasan Habib
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 4 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.4.307-314

Abstract

Snapper is a type of demersal marine fish from the Lutjanidae family. The Lutjanidae family spread throughout the world and currently has 123 species in 21 genera, one of which is the Lutjanus genus (Miller and Thomas, 2007). To this day, the records of capture fisheries production data for snapper in Malang are still very limited to certain types. Morphological identification that has been carried out so far is still challenging to obtain accurate results because of the many similarities between the observed species or the loss of characteristics. Therefore, molecular identification is necessary to determine the types of snappers in this area and their conservation status. This study aims to determine the types of snappers using a molecular approach by Cytochrome Oxidase subunit I (COI) gene marker.  Phylogenetic tree reconstruction and genetic distance calculations were performed using Mega X software through the neighbour-joining (NJ) algorithm. The results of the identification snapper based on a molecular approach with DNA barcoding revealed that the four snapper samples were L. gibbus, L. rufolineatus, L. bengalensis, and L. erythropterus. Based on the results of the compilation of the phylogenetic tree, it can be seen that the L. bengalensis sample is closely related to L. rufolineatus while L. gibbus, and L. erythropterus each form a separate clade from the two previous Lutjanus species. Based on their conservation status at the IUCN, the four species of snapper found are in the Least Concern category.
Pengaruh Konsentrasi Protein yang Berbeda dalam Suspensi Dedak sebagai Pakan terhadap Diameter Kista Artemia salina : Pengaruh Konsentrasi Protein yang Berbeda dalam Suspensi Dedak sebagai Pakan terhadap Diameter Kista Artemia salina Ahmad Shofy Mubarak; Laksmi Sulmartiwi; Muhammad Daffa Al Rasyid Nurhayati
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.278

Abstract

Artemia salina is a natural food that is very important in hatcheries. The need for Artemia as a natural feed for fish or shrimp seeds is high in Indonesia, so it is necessary to develop local Artemia cultivation. One way to do this is to increase the production of Artemia salina cysts by paying attention to the quality and quantity of feed, namely enrichment with ISP (Isolated Soya Protein) flour in the feed given. ISP is a protein isolated from soybeans with the final product in the form of flour, easy to digest and has high nutritional value. Enrichment with ISP flour was carried out with the aim of increasing the protein content that affects the formation of the shell and the size of the good Artemia salina cyst. This study aimed to determine the effect of different protein concentrations using ISP (Isolated Soya Protein) in bran suspension on the diameter of Artemia salina cysts. The research method used was experimental with a completely randomized design (CRD) using 5 treatments and 4 replications. The results showed that different protein concentrations by enriching ISP flour (Isolated Soya Protein) in bran suspension as feed had a significant effect (P<0.05) on Artemia salina cyst diameter in the third week. The administration of ISP with a protein concentration of 40% and 45% showed the highest Artemia salina cyst diameter.
Fekunditas, Diameter Telur, Persentase Fertilitas, Dan Daya Tetas Pada Strain Ikan Cupang (Betta Splendens) Yang Berbeda Darmawan Setia Budi, S.Pi., M.Si; Dian Pebianti; Akhmad Taufiq Mukti; Laksmi Sulmartiwi; Lailatul Lutfiyah; Mohammad Faizal Ulkhaq
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.273

Abstract

Betta fish (Betta splendens) has export and import potential. Several popular betta fish strains include halfmoon, crown tail, double tail, and placard. We used the observation method to observe four betta fish strains with five replications. The four fish strains were spawned and will be dissected to obtain the female betta fish's gonads. The parameters observed in this study were fecundity, egg diameter, fertility, and hatchability. This study's data analysis method was analysis of variance (ANOVA) with Duncan's multiple range test. Based on the findings of the observations, it is known that the results of betta fish spawning and gonadal dissection on these various betta fish strains show a significant difference in fish fecundity (P 0.05). However, no significant difference in egg diameter, fertility, or hatchability was observed (P > 0.05). Betta splendes strains differ in terms of fecundity, fertility, hatching rate, and egg size.
Kadar Glukosa Darah dan Infestasi Ektoparasit Flagellata pada Ikan Mas Koki (Carrasius auratus) yang diberi Suspensi Whole Cell Lernaea dengan Dosis dan Waktu Pemeliharaan yang Berbeda Netty Sreani; Laksmi Sulmartiwi; Gunanti mahasri
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.241

Abstract

The high consumer interest in ornamental fish such as goldfish causes aquaculture to try to meet the highest production so that large scale aquaculture activities appear accompanied by problems due to imbalances between the host, the environment and diseases such as pathogens. The purpose of this study was to determine the effect of giving Whole Cell Lernaea as an immunostimulant development material as an effort to prevent pathogen attacks in aquaculture. This study used a completely randomized design (CRD) with a factorial pattern, namely factor A was different doses of Whole cell Lernaea and factor B was different length of maintenance. The results showed that the highest blood glucose levels of goldfish occurred in the 0 ppm treatment on day 0 with a range between 53.60-87.60 mg/dL and the lowest in the 5 ppm treatment on day 21 with a range between 44.20-86.60 mg/dL. Ichthyobodo spp. ectoparasite infestation. occurred on days 0 and 14 with a dose of 0 ppm which is about 2-3 ind/head different from giving a dose of 5 ppm during maintenance for 21 days there were no cases of goldfish that were attacked by ectoparasites. There was an interaction between dose and duration of maintenance on blood glucose levels of goldfish given Whole cell lernaea suspension, while there was no interaction with flagella ectoparasite infestation. The best treatment to reduce blood glucose levels to normal is the administration of Whole Cell Lernaea suspension at a dose of 5 ppm and a maintenance time of 21 days.
Co-Authors A. Shofy Mubarak Abang Aldhian R. Putera Ade Wahyu Pratama Adrian Damora Ahasan Habib Akhmad Taufiq Mukti Alfian Rahmadhani Satria Hani Saputra Aliffiansyah Rizky Ergion Andi Aliah Hidayani Andre Rekasana Arif Habib Fasya Bernathdo Mahendra Robbi Putra Bintang Eka Nugraha Satia Aditama Boedi Setya Rahardja Budiatin, Aniek Setiya Citra Rachmania Wardhani Clara Amelia Kusumawinahyu DARMAWAN SETIA BUDI Desi Nawang Purnamasari Diah Anggraini Wulandari Diah Trie Ridyaning Tias Dian Pebianti Diana Meritasari Dwi Retna Kumala Ningrum Dwi Yuli Pudjiastuti Dwi Yuli Pujiastuti Dwitha Nirmala Dyah Wahjuning Listyarini Eka Ainurrohmah Eka Saputra Endang Dewi Masithah Fatih Riantono Firdha Farah Pramesti Gunanti Mahasri Hairul Hafiz Bin Mahsol Hari Suprapto Harryes, Regil Kentaurus Herlina Maya Saputri Himna Sayyyidatul Islamiyah Ida Bagus Putu Oka Dantika Indah Permata Sari Isnatul Umu Shohifah Khadijah Zai Kismiyati , Lailatul Lutfiyah, Lailatul Lailatul Mubarokah Mardiah Rahma Umami Mardya Syaifudin S Marwah Sifa Safira Mosyafwa Maulida Agustina Md. Jobaidul Alam Md. Jobaidul Alam Merdeka Agus Saputra Mochammad Amin Alamsjah Mohamad Akmal Alwi Husein Mohammad Faizal Ulkhaq Money Carattri Kusuma Werdani Muhammad Arief Muhammad Daffa Al Rasyid Nurhayati Muhammad Dzaky Muhammad Fauzan Muhammad Rahmad Royan Nazhry Zahra Arifah Netty Sreani Nida'an Khofiyyah Nindhita Yusvantika Norma Isnawati Nova Erika Novian Aji Pradana Oemar Moechthar Oktantia Frenny Anggani Patmawati Patmawati Pradityo Dwi Giartama Prayogo Prayogo Prayogo Pristita Widyastuti Puput Puspitasari Putri Garnet Endo Mahata Rahayu Kusdarwati Raseetha Siva Raseetha Vani Siva Manikam Reza Istiqomatul Hidayah Rizky Aprilia Chrisanti Rr. Juni Triastuti Saltsa Arinda Putri Saniya Lailatul Qodriyah Sapta Wijayanti Sapto Andriyono Selvi Oktora Mahanani Setyantini, Woro Hastuti Sharilla Aryananti Abidin Shofy Mubarok Siska Adelia Sitompul Sri Harweni Sri Subekti Sudarno, Sudarno Syifa Fauziyah Tasyarrafa Naf'a Solakhiyah Tjahjaningsih, Wahju Uswatun Khasanah Veryl Hasan Widjiati Widjiati, Widjiati Yaowapha Waiprib Yenta Kusuma Wardhani Yusma Latifatul Ummah