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Journal : HAYATI Journal of Biosciences

DNA Barcoding of Six Commercially Important Groupers (Epinephelidae) from Langsa, Aceh, Indonesia Fadli, Nur; Jumiati, Sri; Razi, Nanda Muhammad; Damora, Adrian; Muchlisin, Zainal A.; Dewiyanti, Irma; Ramadhaniaty, Mutia; Harnelly, Essy; Habib, Ahasan; Siti-Azizah, Mohd Nor
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.328-335

Abstract

Groupers are among the fish groups that are difficult to recognize due to their high morphological similarities. Therefore, molecular techniques, particularly DNA barcoding, are extensively utilized to differentiate this fish group. This study aimed to analyze and validate six grouper species belonging to the Epinephelidae family that were harvested from Langsa district waters in Aceh province, Indonesia, based on DNA barcode data. It was conducted from June to December 2021, with the fish specimens collected from fishers at fish landing sites and the fish market in Langsa City, Aceh province. A total of 22 grouper sequences belonging to six species were generated, namely Epinephelus coioides, E. bleekeri, E. malabaricus, E. erythrurus, E. sexfasciatus and Mycteroperca poecilonotus (formerly Epinephelus poecilonotus). Genetic distance within these species ranged from 0.10 to 0.73% (average: 0.40%). Notably, E. malabaricus and E. coioides exhibited the closest genetic kinship (4.07%), while E. sexfasciatus and M. poecilonotus displayed the greatest genetic distance (19.33%). This study provides the first DNA reference for grouper in Langsa district, Indonesia, with significant implications for future sustainable grouper management.
DNA Barcoding of Commercially Important Groupers (Epinephelidae) in Simeulue and Banyak Islands, Aceh, Indonesia Fadli, Nur; Razi, Nanda Muhammad; Damora, Adrian; Muchlisin, Zainal A.; Ramadhaniaty, Mutia; Rahayu, Sri Riska; Harnelly, Essy; Habib, Ahasan; Siti-Azizah, Mohd Nor
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1080-1091

Abstract

The groupers are a group of demersal fish that live in tropical and subtropical areas and are mostly linked to coral reefs. The fish are highly valued in international markets and are subjected to overfishing in the wild. Accurate fish resource identification knowledge is essential for sustainable fisheries management. This research is aimed to generate a reference COI sequence library of grouper species caught in Simeulue and Banyak Islands, Aceh. The study was conducted from April-September 2021 at sixteen fish landing sites in Simeulue and Banyak Islands. In total, this study generated 70 COI sequences representing 20 grouper species. Epinephelus (54%) was the most prevalent grouper genus at the study site, followed by Cephalopholis (19%), Plectropomus (13%), Variola (12%), Hyporthodus (1%), and Anyperodon (1%). According to the IUCN classification, 17 species (85%) fall into the category of Least Concern, two (10%) fall into the category of Vulnerable, and one (5%) falls into the category of Data Deficient. The average genetic distance based on the Kimura-2-Parameter (K2P) between specimens was 0.51% at the species level and 8.34% at the genus level. Overall, this study has provided the COI sequence database of grouper for the Simeulue and Banyak Islands.
Co-Authors Adrian Damora Agus Sara Alim Misbullah Amirunnas , Amirunnas Annisa Ammalia Kiti Ardhana Yulisma Arif Habibal Umam Ariqah, Nada Cici Ariska A Dina Firmadiana Fahira, Cut Nathasya Faradhila, Jihan Fauzi, Fazlin Mohd Fauziah . Fenty Ferayanti Fikrul Akhyar Gani, Fadli A. Habib, Ahasan Hairul Basri Hawati Hawati HENDRIX INDRA KUSUMA Hermanto, Feri Eko Idroes, Ghifari Maulana Iqbar Iqbar Irma Dewiyanti Irma Sari ISKANDAR ZULKARNAIN SIREGAR Isnaini, Nadia Itawarnemi, Hilmina Khairan Khairan Kiti, Annisa Ammalia Kusuma, Hendrix Lenni Fitri Lydia Septa Desiyana, Lydia Septa M. Satria Fitri Malahayati T. Hanafiah Martunis - Martunis Martunis Maysarah, Hilda Meutia Zahara Mira Humaira Misrahanum Misrahanum MUHAMMAD ADRIYAN FITRA MUHAMMAD BAHI Muhammad Irfan Muhammad Rusdi Muhammad Subianto Munira, Alya Mutia Zahara Nafissa, Naja Nanda Muhammad Razi Nazaruddin Nir Fathiya NITA TAUHIDA NITA TAUHIDA Nur Fadli Nurur Rahmy Oktaviana, Nurul Onny Ulfa Rahayu Prajaputra, Vicky Rahayu, Sri Riska Ramadhaniaty, Mutia Rauzana, Anita Razief Perucha Fauzie Afidh RIDHA UL FAHMI Rina Sriwati Rinaldi Idroes Rizki Amelia Rizki, Alia Rizky Amelia Rusita Fitri Saida Rasnovi SAIDA RASNOVI Saida Rasnovi Samingan Samingan Sari, Febia Saudah Saudah SITI MARYAM Siti-Azizah, Mohd Nor Sreeramanan Subramaniam Sri Jumiati, Sri Sri Wati Suryadi Suryadi Syaharani, Cut Puspita Salsabila Syahraini, Aigia Syaifullah Muhammad Teuku Rizky Noviandy Ulfa hansri Ar-Rasyid Wira Dharma Wira Dharma Yekki Yasmin Yudi Haditiar Yunita Yunita Yurinda Yurinda ZAINAL ABIDIN MUCHLISIN Zairin Thomy Zairin Thomy Zairin Thomy Zairin Thomy ZAIRIN THOMY Zairin Thomy Zairin Thomy Zairin Thomy Zairin Thomy Zakaria, Rahmad Zulfan Zulkarnain Zulkarnain Zulwanis, Zulwanis Zumaidar Zumaidar Zumaidar