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Journal : Proceeding Biology Education Conference

DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN DAN KULIT BATANG SAWO KECIK (Manilkara kauki L Dubard)TERHADAP BAKTERI Escherichia coli Prayudhani, Maya Firdausi; Hastuti, Utami Sri; Suarsini, Endang
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Potensi Sawo kecik (Manilkara kauki  L. Dubard) sebagai sumber obat alami untuk diare belum terbukti secara ilmiah.Sawo kecik mengandung flavonoid yakni senyawa yang bersifat sebagai antibakteri, sehingga sawo kecik diasumsikan dapat menghambat pertumbuhan bakteri penyebab diare Escherichia coli. Tujuan dari penelitian ini ialah: 1) untuk menganalisis pengaruh perbedaan konsentrasi ekstrak etanol daun dan kulit batang sawo kecik terhadap penghambatan pertumbuhan Escherichia coli secara in vitro, 2) untuk menentukan kombinasi ekstrak etanol daun dan kulit batang sawo kecik dengan variasi konsentrasi yang paling efektif dalam menghambat pertumbuhan  Escherichia coli    secara  in vitro. Daya antibakteri ekstrak etanol daun dan kulit batang sawo kecik dalam konsentrasi 0%, 5%, 15%, 25%, 35%, 45%,  55%, 65%, 75%, 85%, 95% dan 100% diuji terhadap bakteri Escherichia coli secara in vitro dengan metode difusi agar dengan cara Kirby Bauer Test. Antibiotik Levofloxacin  digunakan sebagai kontrol positif.  Data yang diperoleh dibandingkan dengan kontrol positif dan  dianalisis menggunakan analisis varian ganda. Hasil penelitian ini menunjukkan bahwa: 1) perbedaan konsentrasi ekstrak etanol daun dan kulit batang sawo kecik berpengaruh terhadap penghambatan pertumbuhan  Escherichia coli  secara in vitro, 2) ekstrak etanol kulit batang sawo kecik pada konsentrasi  55% merupakan kombinasi yang paling efektif dalam menghambat pertumbuhan  Escherichia coli  dengan rerata diameter zona hambat yang dihasilkan sebesar 12.3 mm (51% terhadap Levofloxacin). Kata kunci: Daya antibakteri, Metode difusi agar, Ekstrak etanol, Sawo kecik (Manilkara kauki  L  Dubard), Escherichia coli.
Observasi Histologik dan Identifikasi Fungi Endofit yang Diisolasi dari Cananga odorata (Lam.) Hook.F. & Thomson Hastuti, Utami Sri; Rakhmawati, Dwi; Sari, Ria Yustika
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Some endophytic fungi live in medicinal plant tissue, include medicinal plant. This research was done to: 1) determine the endophytic fungi position in the Cananga odorata  plant tissue by histological observation; 2) identify the endophytic fungi species isolated from the leaf, twig, and petal flower of C. odorata. The position determination of endophytic fungi in the C. odorata plant tissue is done by microscopic observation. The endophytic fungi were isolated from the leaf, twig, and flower petal of C. odorata  plant by cutted these plant parts, then inoculated on Potato Dextrose Agar (PDA) medium and incubated in 27oC during 7 x 24 hours, each fungi that grow on the PDA medium were isolated, then the morphology and microscopic characteristic of each fungi colony isolates were described. Each endophytic fungi isolates were identified. The research result were:  1) the endophytic fungi position are on the epidermis cell wall and on the stomata guard cell wallof the leaf tissue, on the parenchymal cell wall of the twig tissue, and on the epidermis cell wall of the flower tissue; 2) Nine endophytic fungi species were isolated from the C. odorata leaf, twig, and flower petal are: Nigrospora sphaerica, Colletotrichum alienum,Mycellia sterilia1, C. kahawae, Rhizoctonia sp., C. aotearoa, Micellia sterilia2, C. alatae, dan C. queenslandicum
Kajian Antagonis Trichoderma Spp. terhadap Fusarium Solani Penyebab Penyakit Layu Pada Daun Cabai Rawit (Capsicum frutescens) Secara in Vitro Ningsih, Husdiani; Hastuti, Utami Sri; Listyorini, Dwi
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Penelitan ini bertujuan untuk mengetahui daya antagonis dari beberapa spesies kapang Trichoderma yaitu T. harzianum, T. viride dan T. koningii dalam menghambat pertumbuhan kapang patogen Fusarium solani penyebab penyakit layu pada daun cabai rawit (Capsicum frutescens). Penelitian ini dilakukan secara in vitro di Laboratorium Mikrobiologi Universitas Negari Malang (UM) pada bulan November 2015 sampai dengan Mei 2016 menggunakan rancangan acak lengkap (RAL) dengan 3 perlakuan dan 6 ulangan. Pengujian antagonis dilakukan dengan metode dual culture pada medium PDA. Hasil penelitian menunjukkan bahwa ketiga kapang antagonis yang diujikan memiliki daya antagonis yang berbeda dalam menghambat pertumbuhan kapang F. solani. Daya antagonis tertinggi dimiliki oleh kapang T. harzianum dengan rata-rata persentase daya antagonis sebesar 60,00%, diikuti oleh kapang T. viride sebesar 52,00%, dan kapang T. koningii dengan daya antagonis terkecil yaitu sebesar 43,33%.Kata kunci:        Kajian Antagonis, T. harzianum, T. viride, T. koningii, F. solani, Patogen pada Cabai Rawit
PENGARUH LIMBAH KULIT BUAH KAKAO SEBAGAI CAMPURAN MEDIA TANAM TERHADAP PRODUKTIVITAS DAN KANDUNGAN GIZI JAMUR TIRAM COKLAT (Pleurotus cystidiosus) Imam Mudakir; Utami Sri Hastuti; Fatchur Rohman; Abdul Gofur
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract - The development of the plantation sector has increased impact of which waste wood sawdust and cocoa fruit peel. These wastes can cause environmental problems if not treated, but they would be beneficial if they are used as growing media mixture in oyster mushroom cultivation. Growing media (baglog) oyster mushrooms are supported physically and chemically will affect the productivity and nutrient content of oyster mushrooms. The study aims to analyze and assess the effect of the cocoa fruit peel waste as  growing media mixture on productivity and nutrient content of brown oyster mushroom (Pleurotus cystidiosus) that has been carried out using a completely randomized design (CRD) with 4 kinds of treatment comparisons sawdust and fruit peel waste cocoa, ie SL0(ratio of 100% wood sawdust and 0% fruit peel waste cocoa)/control, SL1 (ratio of 80% sawdust and 20% fruit peel waste cocoa), SL2 (ratio of 75% sawdust and 25% fruit peel waste cocoa) and SL3 (ratio of 70% sawdust and 30% fruit peel waste cocoa). The research data in the forms of number and weight of fruit body production, as well as protein and fat content of brown oyster mushrooms, were then analyzed by one-way anova and followed by DMRT (Duncan Multiple Range Test)  at 5%. The results showed that the use of cacao fruit peel waste by ratio comparison  75% wood sawdust and 25% cacao fruit peel (SL2) is the best to produce the number and weight of fruit body production as well as protein and fat content of brown oyster mushrooms. Therefore, it is recommended to be applied in the cultivation of brown oyster mushroom. Keywords: wood sawdust , growing media, productivity, nutrition, brown mushrooms
Kajian Daya Antagonisme Kapang Trichoderma spp. terhadap Colletotrichum capsici dan Erysiphe cichoracearum Secara In Vitro Dwi Rahmawati; Nurul Yanuarsih; Utami Sri Hastuti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Colletotrichum capsici dan Erysiphe cichoracearum are two species pathogenic molds species on cultivation plants especially vegetables plant. The fungus can be controlled by the antagonist molds Trichoderma spp.. This research used Trichoderma viride, T. atroviride, and T. harzianum as antagonist molds. This research was done for: 1) analize the difference antagonism effect of antagonist molds Trichoderma spp. towards pathogenic molds C. capsici dan E. cichoracearum, 2) determine the Trichoderma species that have highest antagonism effect towards C. capsici danE. cichoracearum, 3) examine the antagonism mechanism of Trichoderma spp. towards kapang C. capsicidanE. cichoracearum based on the microscopic observation. The research methods use dual culture method by inoculate the antagonist mold : Trichoderma viride, T. atroviride, and T. harzianum  and pathogenic mold, are: C. capsici dan E. Cichoracearumcolony pieceson PDA plate medium in pairs, then incubated on 25o-27oC for 4 x24 hours. Afterwards the antagonism effect of Trichoderma spp. towardsC. capsici andE. cichoracearumwere analyzed quantitative descriptive. The antagonism mechanism observed by Scanning Electron Microscope (SEM).  The research results are: 1) there was difference antagonism effect of Trichoderma spp. towards C. capsici dan E. cichoracearum. 2) T. harzianum has the highest antagonism effect towards C. capsici as well as towards  E. cichoracearum, 3) The antagonism mechanism of Trichoderma spp. towards C. capsici and E. cichoracearum by are mycoparasitism,  the Trichoderma hyphae attach, penetrate, and coiling the C. capsici andE. cichoracearum hyphae
Kajian Kualitas Nata de Nira Siwalan (Borassus flabelliver L.) dengan Variasi Macam Gula dalam Beberapa Konsentrasi sebagai Materi Handout Biologi Kelas XII MAN Pamekasan Chandra Kirana; Utami Sri Hastuti; Endang Suarsini
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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The purpose of this study include: (1) analyze the quality of nata de sap of palm with a wide variety and concentration of sugar in terms of weight and fiber content nata; (2) develop a handout materials for students of class XII Biotechnology MAN Pamekasan based on research results; and (3) analyze the quality of handouts developed as the implementation of research results. The research method there are two stages of experiment and development. Research conducted at the Laboratory of Microbiology Faculty UM Malang in January-March 2013. The results showed that: (1) there is a wide influence on the quality of sugar palm sap nata de nata either by weight or fiber content; (2) No effect of sugar concentration on the quality of nata de sap siwalan both based on the weight of nata and fiber content; (3) handout Biotechnology has been eligible for use in helping Biology teaching and learning activities in MAN Pamekasan with excellent qualifications based on an assessment by experts validator Microbiology and excellent materials based on an assessment by experts validator teaching materials.Keywords:        nata de nira siwalan, quality, research based handout
PENGARUH MEDIUM AIR CUCIAN BERAS TERHADAP KECEPATAN PERTUMBUHAN MISELIUM BIAKAN MURNI JAMUR TIRAM PUTIH Sugeng Handiyanto; Utami Sri Hastuti; Sitoresmi Prabaningtyas
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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Biakan murni Jamur tiram putih(Pleurotus ostreatus var. florida) merupakan pembibitan tahap pertama yang berperan sangat penting dalam budidaya jamur tiram. Pertumbuhan miselium biakan murni jamur tiram putih membutuhkan karbohidrat, protein, mineral dan vitamin. Air cucian  beras berpotensi sebagai medium biakan jamur tiram putih karena mengandung karbohidrat, protein, mineral, dan vitamin yang dibutuh-kan dalam pertumbuhan jamur tiram putih. Penelitian ini bertujuan untuk: 1) menguji pengaruh medium air cucian beras dalam beberapa konsentrasi terhadap kecepatan pertumbuhan miselium jamur tiram putih; 2) menentukan konsentrasi medium air cucian beras terbaik yang menghasilkan kecepatan pertumbuhan miselium paling tinggi. Penelitian ini dilakukan di laboratorium Mikrobiologi, jurusan Biologi FMIPA Universitas Negeri Malang pada bulan Februari-April  2013. Jenis penelitian ini ialah penelitian eksperimental. Rancangan yang digunakan ialah Rancangan Acak Lengkap (RAL) dengan perlakuan macam konsentrasi  air cucian beras 0% (sebagai  kontrol negatif), 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, dan medium Potato Dekstrose Agar  (sebagai kontrol positif) yang diulang sebanyak tiga kali. Pengukuran diameter koloni dilakukan pada hari ke-4, hari ke-7 dan hari ke-10 setelah inokulasi. Diameter koloni diukur berdasarkan rerata diameter koloni dengan arah horisontal dan vertikal. Kecepatan pertumbuhan miselium diperoleh dengan cara menghitung selisih diameter akhir dengan diameter awal kemudian dibagi rentang hari.Data  kecepatan pertumbuhan miselium dianalisis dengan menggunakan Analisis Varians Tunggal 5% dan dilanjutkan dengan uji Beda Nyata Terkecil (BNT) 5%.Hasil penelitian ini menunjukkan bahwa: 1)  terdapat perbedaan pengaruhmacam konsentrasi medium air cucian beras terhadap kecepatan pertumbuhan miselium jamur tiram putih; 2) konsentrasi medium air cucian beras terbaik ialah konsentrasi 90% yang menghasilkan kecepatan pertumbuhan miselium jamur tiram putih paling tinggi. Perlakuan kontrol positif dengan medium PDA menghasilkan diameter koloni lebih kecil dibandingkan diameter koloni pada perlakuan medium air cucian beras konsentrasi 90%. Kata Kunci:air cucian beras, kecepatan pertumbuhan, biakan murni, jamur tiram
Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah Hesti Nur Choirunnisa; Ria Yustika Sari; Utami Sri Hastuti; Agung Witjoro
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Wadi is a sort of traditional foods that made of fish from Dayak tribe in Central Kalimantan. Wadi is made bythe addition of salt and lumu. People make wadi as a supply of side dishes during difficult seasons of fish andstock for farming, hunting, or collecting forest products. In wadi, there are several bacteria species that capablein degrading amylum and protein. The bacteria capability in amylum and protein degradation can be determinedby calculating the hydrolysis index. The aims of this study is to: (1) identify the amylolytic bacteria andproteolytic bacteria species from wadi, (2) determine the amylum and protein hydrolysis index on eachamylolytic bacteria and proteolytic bacteria species isolated from wadi, and (3) determine the amylolyticbacteria and proteolytic bacteria species that have the highest hydrolysis index. This research is descriptiveexplorative research. The research results showed that: (1) there were four isolates of amylolytic bacteriaisolated from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Acinetobacter baumannii, andMicrococcus varians, and there were five species of isolated proteolytic bacteria from wadi, i.e: Enterobacteragglomerans, Pseudomonas fluorescens, Nitrococcus mobilis, Acinetobacter baumannii, and Micrococcusvarians, (2) the amylum hydrolysis index of Enterobacter agglomerans is 3.77; on Pseudomonas fluorescensis 3.00; on Acinetobacter baumannii is 4.16; and on Micrococcus varians is 7.23. The protein hydrolysis indexof Enterobacter agglomerans is 3.22; on Pseudomonas fluorescens is 2.25; on Nitrococcus mobilis is 1.67; onAcinetobacter baumannii is 3.42; and on Micrococcus varians is 3.45, (3) Micrococcus varians has the highestamylum, i.e: 7.23 and protein hydrolysis index is 3.45.
ISOLASI DAN IDENTIFIKASI MIKOFLORA KAPANG KONTAMINAN PADA KUE PIA YANG DIJUAL DI KOTA MALANG Utami Sri Hastuti; Yulia Venicreata Dipu; Mariyanti Mariyanti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 8, No 1 (2011): Prosiding Seminar Nasional VIII Biologi
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ABSTRAK Kue pia merupakan salah satu macam makanan yang cukup dikenal oleh masyarakat. Kue pia yang tidak langsung dikonsumsi, seringkali disimpan selama beberapa hari oleh para konsumen. Lama waktu penyimpanan dapat mempengaruhi kerusakan kue pia akibat aktivitas kapang kontaminan. Hal ini dapat mengakibatkan kue pia ditumbuhi oleh kapang, sehingga tidak layak dikonsumsi. Penelitian ini bertujuan untuk : 1) mengetahui spesies-spesies kapang kontaminan yang tumbuh pada kue pia; 2) mengetahui kualitas mikrobiologi kue pia yang disimpan selama 5 x 24 jam berdasarkan Angka Lempeng Total (ALT) koloni kapang. Sampel kue pia diperoleh dari toko kue di kota Malang. Sebelum diberi perlakuan sampel kue pia disimpan dalam botol selai steril selama 5 x 24 jam. Sampel kue pia sebanyak 10 gram dihaluskan dan dilarutkan dalam 90 ml larutan air pepton 0,1% sehingga diperoleh suspensi dengan tingkat pengenceran 10-1. Kemudian suspensi diencerkan lagi dalam larutan air pepton 0,1% secara bertahap sehingga diperoleh suspensi dengan tingkat pengenceran 10-2, 10-3, 10-4, 10-5, 10-6. Suspensi pada masing-masing tingkat pengenceran diinokulasikan pada medium Cazpek Agar (CA) sebanyak 0,1 ml dan diinkubasikan pada suhu 250C selama 7 x 24 jam. Perlakuan sampel dilakukan dalam 3 ulangan. Selanjutnya dilakukan penghitungan Angka Lempeng Total (ALT) koloni kapang pada kue pia yang disimpan selama 5 x 24 jam dan penentuan kualitas mikrobiologi kue pia berdasarkan ALT koloni kapang kemudian dirujukkan pada ketentuan dari DIRJEN POM ; selain itu dilakukan pula isolasi, deskripsi ciri-ciri morfologi dan mikroskopis, serta identifikasi terhadap tiap-tiap spesies kapang kontaminan yang tumbuh pada medium lempeng CA. Hasil penelitian ini menunjukkan bahwa : 1) terdapat 11 spesies kapang kontaminan dalam sampel kue pia, yang termasuk dalam 6 genus, yaitu : Penicillium, Aspergillus, Fusarium, Cladosporium, Trichoderma, Eurotium, dan ordo khusus Mycelia sterilia.; 2) ALT koloni kapang dalam sampel kue pia yang disimpan selama 5 x 24 jam ialah 1,7 x 105 cfu/gram sampel. Hal ini menunjukkan bahwa sampel kue pia yang disimpan selama 5 x 24 jam tidak layak dikonsumsi.   Kata kunci: Mikoflora, kapang kontaminan, kue pia.
Identifikasi dan Penentuan Indeks Hidrolisis Protein pada Bakteri Proteolitik dari Tanah Mangrove di Margomulyo, Balikpapan Utami Sri Hastuti; Febriani Sarwendah Asri Nugraheni; Putri M. Al Asna
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
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Mangrove has a function as a physical protector of the seashore and also play a role as a habitat of various typesof aquatic fauna. The decaying mangrove stems contain vegetable protein and mixed with animal proteinderived from dead animals in mangrove soil. Proteolytic bacteria play a role in the process of proteindegradation into more simple and soluble compounds. This research aims to: 1) identify species of proteolyticbacteria in mangrove soil, 2) count the Protein Hydrolysis Index on each species of proteolytic bacteria, 3)determine the most potential proteolytic bacteria species to decompose the protein. Soil samples were takenfrom 10 points of mangrove area Margomulyo, Balikpapan. After that, soil samples were diluted in 10-1 to 10-10 and 0,1 mL of each level dilution is inoculated to the NA medium, incubated at 37°C for 1 x 24 hours. Eachcolony sorts were isolated, identified and determined the proteolytic bacteria isolates, then the ProteinHydrolysis Index on the Skim Milk Agar medium were count. The research results showed that: 1) there are 7species of proteolytic bacteria from mangrove soil, 2) the Protein Hydrolysis Index of each species ofproteolytic bacteria ranged from 1.62 to 2.94, and 3) Vibrio parahaemolyticus is the most potential proteolyticbacteria with the Protein Hydrolysis Index is 2,94
Co-Authors Abdini, Aulia Abdul Fattah Noor Abdul Ghofur Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Agung Witjoro Ahmad Najib Ajeng Daniarsih Ali Mustofa Ana Syarifatun Nisa Anggia Oktantia Anindya Nirmala Permata Arif Rahman Arini Zahrotun Nasichah Ary Maf’ula Asna, Putri Moortiyani Al Aswal Salewangeng Aziz Tanama Balqis Balqis Betty Lukiati Chandra Kirana Chandra Kirana Chandra Kirana Choirunnisa, Hesti Nur Chomisatut Thoyibah Dina Istia’nah Dwi Listyorini Dwi Rahmawati Dwi Rahmawati Dwi Rakhmawati Dwi Rakhmawati Endang Suarsini Erna Wijayanti Faiza Nur Imawati Ningsih Fatchur Rohman Fatchur Rohman Fatchur Rohman Febriani Sarwendah Asri Nugraheni Febriani Sarwendah Asri Nugraheni Fitri, Rizka Diah Fitria Maulita Fitria Maulita Frida Kunti Setiowati Galina Istiqhfarini Gani, Abdul Rasyid Fakhrun Hadi Suwono Hamidah, Rodiah Amin Hanzen, W.F Edi Hendra Susanto Henny Nurul Khasanah Henny Nurul Khasanah Henny Nurul Khasanah Herawati Susilo Herdina Rahma Zalita Hesti Nur Choirunnisa Ibrohim Imam Mudakir Indah Sari Dewi Indriana Rahmawati Indriana Rahmawati Indriana Rahmawati Intan Rezki Kurniasari Istamar Syamsuri Izzalqurny, Tomy Rizky Izzatinnisa’ Izzatinnisa’ Khusnul Khotimah Kuniasari, Intan Rezki La Arlan Labibah, Sylvana Bilqis Lely Hermawati Linda Hapsari Linda Hapsari M. Ainur Yaqin M. Amin M. Nidhamul Maulana Mafluhah, Luluk Rofiatul Mahesti, Tika Maknuna, Durrotul Mareta Arisswara Edy Mariyanti Mariyanti Mashuri Saputra Mastika, Laily Maghfiro Kamil Maya Firdausi Prayudhani Muhammad Andry Prio Utomo Muhammad Syamsussabri Murni Sapta Sari Natalia Rosa Keliat Naufal Wima Al Fahri Ningsih, Husdiani Nugraheni, Febriani Sarwendah Asri Nursyahbani Saraha Nurul Yanuarsih Nuzalifa, Yossie Ulfa Otavia Dewi Permata Ika Hidayati Pratama, Ade Wahyu Pratama, Aris Yudha Puspitasari, Dela Reni Putri M. Al Asna Putri Moortiyani Al Asna Qorry Aulya Rohmana Rahel Natalia Saragih Munthe Rahmadyah Kusuma Putri Ria Yustika Sari Ria Yustika Sari Ria Yustika Sari Rina Kristina Maria Rizky, Mirza Yanuar Rofiqoh Lailatul Fitriyah Rozana, Kennis Safrudin M. Abidin Saidil Mursali Saidil Mursali Sari, Ria Yustika Siti Aisaroh Siti Annisaa'ul Kariimah Siti Hartina Pratiwi Siti Zubaidah Siti Zubaidah Sitoresmi Prabaningtyas Sri Rahayu Lestari Sueb Sugeng Handiyanto Sugi Hartono Suhadi Suhadi Sulisetijono Sulisetijono Sundari, Syifa Syafitri, Nur Laila Umi Lestari Umu Fatonatul Hidayah W. F. Edi Hanzen W.F Edi Hanzen Wa Ode Nurhawa Yahmi Ira Setyaningrum Yessi Hermawati Yesy Maulina Nadhifah Yheni Sapitri Yulia Venicreata Dipu Yulia Venicreata Dipu Yunita Putri Irsadul Ummah Yunita Rakhmawati Zahida, Nadila Sekar