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Journal : Journal of Global Pharma Technology

Hepatitis B Virus Surface Antigen Subtype in Positive HBsAg Subjects in Mengwi District, Badung, Bali-Indonesia Made Agus Hendrayana
Journal of Global Pharma Technology Volume 10 Issue 12.
Publisher : Journal of Global Pharma Technology

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Abstract

Indonesia is a country with a high prevalence of hepatitis B virus infection and can develop into chronic hepatitis in the community. Indonesia is a multiethnic country with a vast territory, so it is estimated that the prevalence of each hepatitis B virus subtype varies greatly in different regions. Sequences in the S region gene encoding amino acids can show hepatitis b virus subtypes through exclusive variations in subtype determinants of HBsAg. This hepatitis B virus subtype is epidemiologically important, because it can show differences in geography and ethnicity in its spread. This study aims to analyze the hepatitis B virus subtype in HBsAg positive subjects in Mengwi, Badung, and Bali-Indonesia. The subjects of the study were 75 patients who came to the Mengwi I Badung Primary Health Center. All serum samples were examined for HBsAg by the ELISA method. In HBsAg positive serum samples, hepatitis B virus subtypes are determined. The DNA is extracted from serum samples that are HBsAg positive. Amplification of portions of DNA S gene from serum HBsAg positive was performed by polymerase chain reaction first-round and second-round. The nucleotide sequence of the hepatitis B virus is converted into amino acid sequences and multiple alignments are performed. The hepatitis B virus subtype was determined by amino acid substitution analysis at positions 122, 127, 134, 159, 160 and 177 in the S gene assisted by using a computer program. The subjects with HBsAg positive were obtained from 11 out of 75 samples (14.7%). The results of hepatitis B virus subtype analysis found that almost all samples, 10 of 11 samples (90.9%) had adw2 subtypes, while one sample (9.1%) had adrq + subtype.Keywords: HBsAg, Subtype, HBV, Mengwi.
Co-Authors Agastiya, I Made Cahyadi Agus Eka Darwinata Amy Yelly Chandra, Katherine Silvania Chindyvita Darung, Maria Dennis Yulianto Desak Made Hari Wijayanti Dewa Ngurah Suprapta Dewa Ngurah Suprapta Dwija, Ida Bagus Nyoman Putra Gandari, NK. Matalia Govinda Vittala Gusti Ngurah Juniartha Handayani, I Gusti Ayu Nastiti Wahyu HARI MULYAWAN Hearty Indah Oktavian Hendrawan, Gresya I Dewa Ayu Made Dian Lestari I Dewa Ayu Rayna Nareswari Wikananda I Dewa Gde Bagus Panji Palaguna I Dewa Made Sukrama I G A Aristi Dhika Pranani I G. A. Ngurah Aswin Panji Sanjaya I Gede Agus Darsana Palgunadi I Gede Bayu Adi Pratama I Gede Pradnya Wisnu Murthi I Gede Raka Adhyatma I Gusti Ngurah Juniartha I Kadek Aditya Nugraha I Kadek Serisana Wasita I Made Ady Wirawan I Made Jawi I Made Nugraha Gunamanta Sabudi I Made Pande Dwipayana I Made Reza Pramudya I Made Sathya Vijayananda I Made Wira Kusuma I P Ananta WS I Putu Bayu Mayura, I Putu Bayu I Wayan Windi Artha I.A.A. Widhiartini Ichlazul Ma’ruf Ida Ayu Andhira Dewi Suarisavitra Ida Bagus Aditya Bhaskara Ida Bagus Nyoman Putra Dwija Ida Bagus Nyoman Putra Dwija Ida Sri Iswari IGM Wijaya P IGP Dhinarananta IP Ananta WS Kadek Adit Wiryadana Kadek Surya Atmaja Ketut Indah Karina Mahardika Komang Januartha Putra Pinatih Kusumajaya, I Gusti Bagus Ari Adi Laksmi, Ida Ayu Agung LUH MERTASARI . Luh Seri Ani Luh Seri Ani Made Indra Wijaya Made Kurnia Widiastuti Giri Made Kurnia Widiastuti Giri Made Widianantara Nathasia Ngurah Agus Sanjaya ER Ni Kadek Fiora Rena Pertiwi Ni Komang Sulyastini Ni Made Dian Kurniasari Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Sri Budayanti Ni Putu Tesi Maratni Ni Wayan Mega Sri Wahyuni Ni Wayan Prabasiwi Kirana Nila Wahyuni P Ananta WS P Yuniadi A Paramyta, I Gusti Ayu Cintya Pramadya PN Putra, I Dw. Gd. Bayu Artha Pratama Putu Agung Satvika Pradnyadevi Putu Gitanjani Mahadewi Semadhi Putu Harrista Indra Pramana Putu Yuniadi Antari Rena Sari Megayanthi Santhi Y, Ni Putu Kusuma Saranova, Hilda Saraswati P. Yogita Sieny Veronica Sonia Elvira Salim Vittala, Govinda Wahyuni, Ni Wayan Mega Sri Wayan Citra Wulan Sucipta Putri Wibisana, I Dewa Nyoman Adi Ningrat Winatha, I Gde Pangestu Putrama Winaya, Made Illene Wira Guna, I Gede Bhima