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Journal : BERITA BIOLOGI

REGENERASIPROTOPLAS TANAMAN TERUNG DAN KETAHANAN REGENERAN TERHADAPPENYAKIT BAKTERILAYU Husni, Ali
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.025 KB) | DOI: 10.14203/beritabiologi.v7i6.862

Abstract

Protoplasts regeneration was studied on 3 cultivars of eggplant (pupel, green, ang white fruits) and the regenerant were tested for their resistance to bacterial wilt. The researh was conducted in different steps, including protoplast isolation, protoplast purification, protoplast culture, cell suspention dilution, shoot regeneration, plantlet aclimatization, and testing for the resistant to bacterial wilt. Observations were made for protoplast number of each cultivar, protoplast cell wal formation, cell division, number of cell colony, microcalli, and shoot, percent of plantlet survival, in acclimatization, and the resistance of regenerants to bacterial wilt. Data was analized by calculating the average, percentage, and standart deviation. Results show that the combination of 0.5% cellulase Onozuka RS, 0.5% macerozyme R-10, 0.05% MES, and 9,1% mannitol in CPW solution was able to isolateds alot of sprotoplast (10 ) in each cultivars. The first culture incubation with out lighty in both (KM8P and VKM) media and reach which 0,2 mg/1 2,4-D, 0,5 mg/1 zeathin, 1 mg/1 IAA, promoted the formation of cell wall and cell division developed to microcalli. Regeneration media of MS, applied with vitamin MW, 0,1 mg/1 NAA, and 2 mg/1 zeathine produced 13 shoots.. From 21 regenerants acclimatized only 15 regenerants survive. There were differences among the regenerants in their resistance to bacterial wilt,3 regenerants resistant, 2 regenerant recovery (tolerant) and 10 regenerants were susceptibel.
Co-Authors Adhitia, Fabian Agus Joko Santoso Agus Purwito Annisa, Ajeng Nur Antika, Dea Mela Ariesta, Wiwin Arif Qisthon Arzakiyah, Fina Badruttamam, Badruttamam Baroya, Mushlihatun Beninda, Adellia Budiman, Edy Budiyanto, Ferly Rahmat Chaireni Martasari Dakhlan, Akhmad Daniatur, Arya Desyandri Desyandri Dewi Sukma Dewi, Anggi Derma Tungga Dian Puji Rahayu Dian Septinova Diny Dinarti Disa, Putra Rama edy santosa, purnama Eka Rosyida Erwanto Erwanto Farida Fathul Fathur Rahmi, Atika fatimah Fatimah Febriyanti, Leni Nur Fitri Yulianti Fransiska Hamdani, M Dima Iqbal Hobir, NFN Ika Mariska Ilhamsyah, Alifudin Indonant, Hassem Muhamad Inka Aulia Karsinah - Karyanti ,, Karyanti Kirani, Wahyu Sasi Kohir, Muhammad Zahir Alwan Komang Trisna Wirakusuma KRISTIANTO NUGROHO, KRISTIANTO Kurniantha, Muhammad Aldi Kusuma Adhianto Liman Liman Liman, Liman M. Kosmiatin Madi Hartono Maemunah Maemunah Maliya, Mirwa Siti Maulidiyah, Salamatul Mia Kosmiatin Muhammad Habibi Muhammad Mirandy Pratama Sirat Muhtarudin Musthofa, Arfan Fahrizki Permata Sari, Lilis Pramana, Weldi Adi Pratama Adi Prayoga Pratama, Ridho PUJI LESTARI Purnama Edy Santosa Putra, Alvin Permana Putri Pramudita Putri, Elly Yani Medyas Raden Mohamad Herdian Bhakti Ramadani, Fajar Ratna Ermawati Reni Indrayanti Reza Ramdan Rivai Rosita Rosita Rr Riyanti, Rr Rudy Sutrisna Samliok Ndobe Saputri, Nenti Sari, Berliana Setio, Slamet Setyadi, Hario Jati Slamet Susanto Sukmadjaja, D. Sulastri Sulastri Sulistiawan, Dicky Syinwani, Billy M Taruk, Medi TRI JOKO SANTOSO Tri Muji Ermayanti Triyani Dewi Veithzal Rivai Zainal Veronica Wanniatie Widiasri, Ni Luh Putu Widiawati, Arlintia Wulandari, Dyah Retno Wulandari, Dyah Retno Zakirah Raihani Ya’la