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All Journal Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Media Kedokteran Hewan ARSHI Veterinary Letters Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Buletin Veteriner Udayana
i Nengah Wandia
Laboratorium Anatomi Bagian Anatomi Fakultas Kedokteran Hewan Universitas Udayana, Denpasar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Polimorfisme Genetik DNA Mikrosatellite GEN BoLA Lokus DRB3 pada Sapi Bali (Bos indicus) I Ketut Puja; I Nengah Wandia; Putu Suastika; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.116

Abstract

Tujuan penelitian ini adalah untuk mendapatkan informasi dasar mengenai distribusi frekuensi lokus DRB3 gen BoLa (bovine lymphocyte antigen) pada sapi Bali. Untuk isolasi DNA digunakan sampel darah sapi Bali yang diambil dari populasi sapi Bali yang berasal dari Bali dan sapi Bali yang berasal dari Nusa Penida. Jumlah sampel untuk sapi Bali yang berasal dari Bali adalah 22 ekor dan sapi yang berasal dari Nusa Penida 21 ekor. Jumlah allel lokus DRB3 pada sapi Bali asal Bali adalah 7 dan 9 allel dari sapi Bali asal Nusa Penida. Rataan heterozigositas perlokus adalah 0,7967 pada sapi Bali asal Nusa Penida dan 0,7863 pada sapi Bali asal Bali. Nilai PIC lokus DRB3 pada sapi Bali asal Nusa Penida adalah 0,7417 dan 0,742 pada sapi Bali asal Bali. Dapat disimpulkan dari hasil penelitian ini adalah lokus DRB3 pada sapi Bali sangat polimorfik.
Karakteristik Mikrosatelit Gen BoLA dengan Penanda Primer RM 185 pada Sapi Bali (Bos indicus) di Nusa Penida Putu Suastika; I Ketut Puja; I Nengah Wandia; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i2.131

Abstract

Penelitian ini bertujuan menjelaskan distribusi frekuensi lokus gen bovine lymphocyte antigen (BoLA) memakai primer RM 185 pada sapi bali dari Nusa Penida. Sebanyak 21 ekor sapi bali yang berasal dari Nusa Penida diambil secara acak. Jumlah alel gen BoLA pada lokus RM 185 ada 7 macam alel yaitu 76 pb, 84 pb, 86 pb, 90 pb, 98 pb, 100 pb, dan 104 pb. Frekuensi tiap- tiap alel yang teramati adalah 0,02%; 0,09%; 0,31%; 0,07%; 0,12%; 0,28%; dan 0,09%. Rata- rata heterozigositas yang didapat adalah 0,804. Dapat disimpulkan, bahwa lokus RM 185 pada gen BoLA sapi bali di Nusa Penida adalah sangat polimorfik di antara populasi.
ASOSIASI POLIMORFISME GENETIKA LOKUS DEOXYNUCLEIC ACID (DNA) MIKROSATELIT GEN BOVINE LYMPHOCYTE ANTIGEN (BoLA) DENGAN KUALITAS SEMEN PADA SAPI BALI I Ketut Puja; I Nengah Wandia; Putu Suastika; I Nyoman Sulabda
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.094 KB) | DOI: 10.21157/j.ked.hewan.v7i2.932

Abstract

Tujuan penelitian ini adalah mendapatkan informasi dasar mengenai struktur genetika menggunakan marka melokuler DNA mikrosatelit gen bovine lymphocyte antigen (BoLA) dan hubungannya dengan kualitas semen sapi bali. Data diambil dari 8 ekor sapi jantan yang ada di Unit Pelaksana Teknis Daerah (UPTD) Inseminasi Buatan, Baturiti, Tabanan, Bali. Hasil pemeriksaan kualitas sperma menunjukkan bahwa volume semen sapi bali 3,0-6,5 ml, konsentrasi spermatozoa 800-11.000 juta sel/ml, dan persentase spermatozoa motil 70-75%. Hasil penelitian pada sapi bali menunjukkan bahwa ketiga lokus mikrosatelit yang digunakan teramplifikasi pada sapi bali dan jumlah alel pada lokus RM185 dan BM1815 adalah 2 sedangkan jumlah alel pada lokus DRB3 adalah 1. Heterozigositas per lokus berkisar 0-0,533. Nilai PIC per lokus antara 0-0,375. Dari hasil penelitian ini dapat disimpulkan bahwa ukuran alel pada ketiga mikrosatelit berpotensi sebagai marka molekuler yang berperan terhadap kualitas semen pada sapi bali.
Macroscopic Features of the Intugementary System of Geriatric Kintamani Dogs Indrayani, Ni Putu Anggita; Wandia, I Nengah; Gunawan, I Wayan Nico Fajar; Jayanti, Putu Devi
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.212-219

Abstract

Observing the health of Kintamani dogs, especially at geriatric age, is important as an effort to preserve their lives. Observations of the integumentary system can provide information related to clinical animal health. This study aims to determine the integumentary condition of Kintamani dogs macroscopically at geriatric age. This study used 5 Kintamani dogs of geriatric age with a healthy clinical condition. The research procedure was carried out by macroscopic observation of hair and skin by inspection and palpation methods. The results showed that the hair of geriatric dog samples experienced changes in color and texture when compared to productive age Kintamani dogs. Observation of skin flexibility showed that the skin flexibility of geriatric dogs was lower when compared to productive-age Kintamani dogs. Observations of the hair strength of Kintamani dogs showed that at the average geriatric age it was difficult to pull out during manual combing with the observer's hand.  This observation shows that the integumentary system of geriatric Kintamani dogs is macroscopically not much different from productive-age Kintamani dogs.
Hemangioma vulva pada anjing Kintamani Sewoyo, Palagan Senopati; Teja, Putu Tessa Hariys Septianda; Wandia, I Nengah; Purbantoro, Steven Dwi
ARSHI Veterinary Letters Vol. 7 No. 2 (2023): ARSHI Veterinary Letters - Mei 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.2.23-24

Abstract

Hemangioma is a common benign tumor that originates from blood and lymphatic vessels, occurring in both humans and animals. In dogs, these tumors are primarily found on the skin. This case report highlights the first reported incidence of vulvar hemangioma in a Kintamani dog. The complete blood count showed the animals had leukocytosis. Total excision of the tumor mass was performed, and postoperatively, the dog was given analgesic and antibiotic with tolfenamic acid and amoxicillin, respectively for 5 consecutive days. Topical antibiotic neomycin sulfate was also given on the surgical site. Prognosis for this case was considered good based on the nature of the tumor and its location. Based on postoperative monitoring, it can be concluded that the treatment was successful without any recurrence.
ASOSIASI POLIMORFISME GENETIKA LOKUS DEOXYNUCLEIC ACID (DNA) MIKROSATELIT GEN BOVINE LYMPHOCYTE ANTIGEN (BoLA) DENGAN KUALITAS SEMEN PADA SAPI BALI Puja, I Ketut; Wandia, I Nengah; Suastika, Putu; Sulabda, I Nyoman
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.932

Abstract

Tujuan penelitian ini adalah mendapatkan informasi dasar mengenai struktur genetika menggunakan marka melokuler DNA mikrosatelit gen bovine lymphocyte antigen (BoLA) dan hubungannya dengan kualitas semen sapi bali. Data diambil dari 8 ekor sapi jantan yang ada di Unit Pelaksana Teknis Daerah (UPTD) Inseminasi Buatan, Baturiti, Tabanan, Bali. Hasil pemeriksaan kualitas sperma menunjukkan bahwa volume semen sapi bali 3,0-6,5 ml, konsentrasi spermatozoa 800-11.000 juta sel/ml, dan persentase spermatozoa motil 70-75%. Hasil penelitian pada sapi bali menunjukkan bahwa ketiga lokus mikrosatelit yang digunakan teramplifikasi pada sapi bali dan jumlah alel pada lokus RM185 dan BM1815 adalah 2 sedangkan jumlah alel pada lokus DRB3 adalah 1. Heterozigositas per lokus berkisar 0-0,533. Nilai PIC per lokus antara 0-0,375. Dari hasil penelitian ini dapat disimpulkan bahwa ukuran alel pada ketiga mikrosatelit berpotensi sebagai marka molekuler yang berperan terhadap kualitas semen pada sapi bali.
Surgical Removal of Air Rifle Projectiles in Long-Tailed Macaques (Macaca fascicularis) in the Ubud Monkey Forest Sewoyo, Palagan Senopati; Wirata, I Wayan; Gorda, I Wayan; Wardhita, Anak Agung Gde Jaya; Bebas, I Wayan; Soma, I Gede; Putra, I Gusti Agung Arta; Wandia, I Nengah; Brotcorne, Fany
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.158-162

Abstract

Background: Long-tailed macaques (Macaca fascicularis) frequently share their habitat with humans, leading to conflicts, especially when habitat disturbances force them to exploit human food sources. In response, humans sometimes use air rifles to deter these animals. Preventive measures such as sterilization programs, habitat restoration, and public education about responsible interactions with macaques are essential to reduce the occurrence of such incidents and promote coexistence. Purpose: This report describes the anesthesia and surgical management of gunshot wounds in long-tailed macaques. Case(s): Two adult free-ranging male long-tailed macaques in the Ubud Monkey Forest, Bali, Indonesia, were presented with gunshot wounds on the inguinal region and right caudal pelvic area, respectively. These macaques were captured and anesthetized for a scheduled vasectomy. A detailed clinical examination indicated that the projectiles were lodged in the subcutaneous tissue. Based on their shape and size, the projectiles were suspected to be 0.177 caliber (4.5 mm) air rifle pellets. Case Management: Surgical removal was performed to extract the air rifle pellets. Preoperatively, the macaques received amoxicillin (15 mg/kg BW, IM), ketorolac (2.5 mg/kg BW, IM), and Visine® eye lubricant. Propofol was used for anesthetic maintenance (1 mg/kg BW, IV). Following pellet removal, the wound was sutured with monofilament absorbable poliglecaprone 25, and the planned vasectomy was conducted using the bilateral incision method. Postoperatively, the macaques were administered atipamezole (0.02 mg/kg BW, IM) for anesthesia reversal, placed in recovery cages in lateral recumbency, and closely monitored. Conclusion: Once the macaques showed clear signs of recovery from anesthesia, they were transported back to the capture site and released into their original group.
CASE REPORT: TREATMENT OF OBLIQUE DIAPHYSIS FEMUR FRACTURE IN A 3-MONTH-OLD LOCAL DOG Devi, Made Vidia Saraswati; Pemayun, I Gusti Agung Gde Putra; Wandia, I Nengah
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p27

Abstract

Femoral fracture is damage or loss of continuity of femoral bone tissue with or without displacement of bone fragments. Femoral fractures are often caused by mechanical trauma. A 3-month-old crossbreed dog, weighing 4 kg and male, complained of swelling, unable to walk and the right leg could not support. Based on owner information, the case dog was hit by a vehicle two days earlier. The results of the radiographic examination, the dog had an oblique fracture of the right femoral diaphysis with a prognosis of fausta. Treatment was carried out by surgery using internal fixation of intramedullary pins using general anaesthesia, a combination of xylazine and ketamine. Postoperatively, the antibiotic cefotaxime was given at a dose of 20 mg/kg/q12h (IV), followed by cefadroxil syrup at a dose of 25 mg/kg/q12h analgesic meloxicam tablets at a dose of 0.1 mg/kg/q24h. One week after surgery, a radiographic examination was carried out, the results showed that the pin was pushed up. The second operation was performed to correct the installation of the first pin. After the second operation, the antibiotic amoxicillin syrup (R/Amoxsan) was given at a dose of 20 mg/kgq8h, analgesic mefenamic acid tablets at a dose of 30 mg/kg/q12h and calcium carbonate (R/Osteocal) at a dose of 66 mg/kg/q24h to accelerate the callus formation process. Thirteen days after the operation, the wound had dried well and the dog was able to set its legs and run although it was still limping. The results of the X-Ray radiography thirty-four days after the operation showed that callus had formed on the fracture fragment of the femur bone. It is recommended that surgery be performed immediately to avoid the formation of callus at the end of the fracture and after the installation of the intramedullary pin, periodic radiographic examinations are carried out to determine the position of the pin in the bone marrow.
Morphology of Erythrocytes and Leukocytes of the Lesser Sunda Islands Pit Viper (Trimeresurus insularis): The Green Coloration from Bali Island and the Blue Coloration from Komodo Island Diza Pakom, Muhammad Sofi; Wandia, I Nengah; Setiasih, Ni Luh Eka; Komala Sari, Tri; Kardena, I Made; Suartini, I Gusti Ayu Agung
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.111802

Abstract

Indonesia is a tropical country with a diverse range of herpetofauna, one of which is the Trimeresurus insularis snake, a highly venomous species endemic to Indonesia. This snake is distributed across Java, Bali, West Nusa Tenggara, East Nusa Tenggara, and Wetar Island in Maluku Province. Despite its wide distribution, this species exhibits variations in body coloration. T. insularis from Bali is green, while those from Komodo Island are blue; however, both share the same red tail. This study aims to examine the morphology and morphometry of erythrocytes and leukocytes in T. insularis from different localities and color variations. The research utilized blood smear preparations from six healthy T. insularis individuals—three green coloration from Bali and three blue coloration from Komodo Island—analyzed using a microscope and Optilab Viewer camera. Cell morphometry was measured using ImageJ software. The morphometric measurements of erythrocytes included the length and width of the cytoplasm and nucleus, while leukocyte morphometry covered the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, azurophils, lymphocytes, and monocytes. The erythrocyte morphology of T. insularis from both Bali and Komodo Island showed no differences. Morphometric data were analyzed using the Independent-Samples T Test. The results showed significant differences in the length and width of the erythrocyte nucleus, as well as in the cytoplasmic and nuclear diameters of lymphocytes. Meanwhile, no significant differences were found in erythrocyte length and width, as well as in the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, and monocytes.
Co-Authors Abd. Rasyid Syamsuri Agus Wawan Darmawan Aida Lousie Tenden Rompis Alda dasril lumban gaol An'nisafitri Lutviana Anak Agung Ayu Mirah Adi Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Wisnu Kusuma Putra Andini, Ni Made Ria Ayu Ratnasari BASYOFI DWIWANDANA Bellantari, Melinda Brotcorne, Fany Darmawan, I Putu Gede Buda Daud Steven Triyomi Hariyanto Debora Selfia Br Manurung Devi, Made Vidia Saraswati DEWA AYU PUTU ARIE SERATHAN SUPARTHA Dini Maharani Diza Pakom, Muhammad Sofi Evi kumala dewi Febio Tomasini Marciano Meus Fedri Rell Gde Angga Caka Primanditha, Gde Angga Caka Gunawan, I Wayan Nico Fajar Gurning, Santri Devita Sari Hasanah, Putri Nur Hidayah, Dinda Nur I GA Artha Putra I Gede Soma I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Gede Putra Pemayun I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I K. SUATHA I Ketut Anom Dada I Ketut Puja I Ketut Suatha I Made Bagus Arya Permana Ardiana Putra I Made Dwinata I Made Kardena I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Nyoman Sulabda I Putu Gede Yudhi Arjentinia I Wayan Batan I Wayan Bebas I Wayan Gorda I Wayan Wirata I. B. WINDIA ADYANA Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Indrayani, Ni Putu Anggita Jefri Ndawa Rumba Made Rahayu Kusumadewi Made Raysa Merliana Maharani, Nisa Mahmud Siswanto Mahmud Siswanto MIKEU PAUJIAH MUR MUAZIN MUAZIN Muhadjir, Iin Mutmainnah Murdayasa, I Wayan Gde Ni Luh Eka Setiasih Ni Luh Made Ika Yulita Sari Hadiprata Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Wayan Patmawati Ni Wayan Patmawati Ni Wayan Patmawati Nugraha, Elisabeth Yulia Purbantoro, Steven Dwi Putu Devi Jayanti Putu Suastika Rahmiati, Nur Ilmi Risha Catra Pradhany Rostiani Silta RUSMI AKIRA S. K. WIDYASTUT Saputra, Muhammad Rama Imam Saulina, Renata Sendana, Lois Sewoyo, Palagan Senopati Sihombing, Tri Indra Erikson Silaban, Root Elisa Sri Kayati Widyastuti Suastika , Putu Teja, Putu Tessa Hariys Septianda Theresa Utami Tjokorda Sari Nindhia TRI KOMALA SARI WAODE SANTA MONICA Wibisono, Hanif Wahyu Wulandari Wulandari Yesy Febnica Dewi Yulianty, Syifaurrachmah ZAKIATUN MUHAMMAD