Article Per Year (5 Year)
p-Index From 2021 - 2026
2.998
P-Index
This Author published in this journals
All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Jurnal Penelitian Saintek WARTA Biomedika Pharmacon Majalah Obat Tradisional Journal of Nutraceuticals and Herbal Medicine Martabe : Jurnal Pengabdian Kepada Masyarakat JFIOnline AL MA'ARIEF : JURNAL PENDIDIKAN SOSIAL DAN BUDAYA Jurnal Farmasi Jurnal Farmasi dan Sains Indonesia (JFSI) Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Farmasi Sains dan Praktis Prosiding University Research Colloquium Prosiding Seminar Informasi Kesehatan Nasional Journal of Pharmaceutical and Sciences. Usadha Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Kefarmasian Indonesia
Peni Indrayudha
Universitas Muhammadiyah Surakarta
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

Published : 41 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 41 Documents
Search

 1   2   3   4   5 
AKTIVITAS SITOTOKSIK EKSTRAK ETANOL, FRAKSI ETANOL-AIR, ETIL ASETAT SERTA N-HEKSANA BUAH PARE (MOMORDICA CHARANTIA) PADA SEL MCF-7 SECARA IN-VITRO Pamungkas, Adhe Retnantya; Indrayudha, Peni
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 2 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i2.9049

Abstract

Bitter melon is one of fruit that have pharmacological effects such as an anticancer which potentially active on breast cancer cell MCF-7. The aim of this study was to compare the potential cytotoxic activity between ethanol extract of pare and the results of ethanol, ethyl acetate and n-hexane fraction on MCF-7 breast cancer cells and to determine the class of compounds contained in each sample. Pare?s powder was extracted with maceration method in 80% ethanol solvent and then continue to fractionated in 96% ethanol, ethyl acetate and hexane solvent. Extract and fractions were continue to cytotoxic assay using the MTT assay method. Cytotoxic test results showed that the ethanolic extract had no potential cytotoxic activity. Ethyl acetate fraction with the highest concentration 16 µg/mL has the highest potential inhibition 43,87% on MCF-7 cells population. Extract and fractions than continue to phytochemical screening with Thin Layer Chromatography (TLC) method. TLC detection showed that n-hexane fraction contained more compound groups than ethanolic extract, ethanolic-water fraction and ethyl acetate fraction.
Cytotoxic Assay of Semipolar Fraction Of Ethanolic Extract From Sugar Apple (Annona Squamosa L.) Stem Bark on T47D Cells Muflihah, Cita Hanif; Haryoto, Haryoto; Indrayudha, Peni
Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 2 (2020)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v17i2.12268

Abstract

Previous research has shown that some compounds in leaves and seeds of sugar apple have a cytotoxic activity. The aim of this research was to determine the cytotoxicity of semipolar fraction of ethanolic extract from sugar apple stem bark (Annona squamosa L.) on T47D cancer cells. The semipolar fraction of ethanolic extract from sugar apple stem bark was collected by fractionation using Vacuum Liquid Chromatography (VLC) with hexane:ethyl acetic (9:1, 8:2, 7:3, and  6:4)  as  mobile  phase.  Cytotoxicity from the fractions of five different concentration namely; 25, 50, 100, 150, and 250, µg/mL was measured by MTT assay. The potency of the cytotoxicity was defined by the ability of the fraction to inhibit the growth of T47D cells indicated by the value of IC50. Qualitative analysis of contained compounds in the fraction was done by Thin Layer Chromatography (TLC) method using silica gel F 254 as a stationary phase and hexane:ethyl acetic (7:3) as a mobile phase. UV 254 and 366 nm lamp also Dragendorff, citroboric, and FeCl3 spray reagents were used to visualize the spots of the secondary metabolites. The result proved that the semipolar fraction of ethanolic extract from sugar apple stem bark showed potential cytotoxicity on T47D cancer cells with IC50value of 70,77 µg/mL. Qualitative analysis showed that the fraction contained flavonoids and alkaloids which is presumably responsible for its cytotoxic activity. 
FORMULASI SUSPENSI DOKSISIKLIN MENGGUNAKAN SUSPENDING AGENT PULVIS GUMMI ARABICI: UJI STABILITAS FISIK DAN DAYA ANTIBAKTERI Nur Chasanah; Ika Trisharyanti DK; Peni Indrayudha
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 1 No. 2 (2011): Oktober 2010 - Maret 2011
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/far.v1i2.1165

Abstract

Suspension is a dosage contain solid drug material with insoluble form and smooth. Doxycycline is an antibiotic which difficult dissolve in water. Stable doxycycline suspension during 2 week if it kept at space temperature, stable on the air but will change to be dark if it influence sunshine. The aim of the research to evaluate physical stability and ability antibacterial stability doxycycline suspension with PGA as suspending agent. Research category are: Independent variable : concentration of suspending agent (PGA), suspension holding time. Dependent variable: physical stability (sedimentation volume, viscosity, redispersibility, easy to pour, and particle size) and antibacterial of doxycycline suspension, and variabel control is temperature suspension making, create of suspension. Data collecting with measuring physical stability and ability antibacterial of doxycycline suspension. The result shows doxycycline suspension with suspending agent PGA had physical stability and antibacterial ability is good. Physical Stability test show formula 1 rate PGA 5% is formula had physical stability better than formula 2 with rate PGA 7,5% and formula 3 withrate PGA 10%. stability ability test antibacterial show formula 2 is the best in pursues Staphylococcus aureus.
PELATIHAN PEMERIKSAAN TAJAM PENGLIHATAN PADA SISWA KELAS 5 SD GEDONGAN I, COLOMADU, KARANGANYAR Em Sutrisna; Dedi Hanwar; Peni Indrayudha; Tanti Azizah Sujono
WARTA LPM WARTA Volume 10, Nomor 1, Maret 2007
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/warta.v10i1.3210

Abstract

Seeing is crucial aspect in life includes in the field of education. Ifit is not cured the reduction of  sharpness of seeing/visus can affect thestudent ability to study. This action is done to detect  this reduction early.Therefore, the eyes will not be getting worse. The research is done bytraining and demonstrating how to check visus. The location of the researchis SD Gedongan I, Gedongan, Colomadu, Karanganayar. The 31 studentsare checked. The result is that there are 3 students have visus with miop.All the students can participate in this research well.
PEMISAHAN SENYAWA-SENYAWA YANG BERSIFAT SITOTOKSIK TERHADAP SEL MURIN LEUKEMIA P388 DARI EKSTRAK METANOL KULIT BATANG DIPTEROCARPUS CONFERTUS SLOOT (DIPTEROCARPACEAE) . Muhtadi; Peni Indrayudha
Biomedika Vol 5, No 1 (2013): Biomedika Februari 2013
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v5i1.269

Abstract

Six compounds, KP-1 up to KP-6 were isolated from the methanol extract of the tree bark of Dipterocarpus confertus Sloot (Dipterocarpaceae). The cytotoxic activities of these compounds were evaluated against murine leukemia P388 cells. Result of these examination indicated that KP-2 compound and KP-1 were very active with each IC 50 value of these compounds were 2.25 and 5.1 μg/mL, respectively. While the others were not active against murine leukemia P388 cells.Keywords: Dipterocarpaceae, Dipterocarpus confertus Sloot, isolated compounds, cytotoxic, and leukemia P-388 cell
ACTIVITY OF SITOTOKSIK ETANOL EXTRACT PLANT GROWTH (Cynometra ramiflora Linn) ON HEAVY CELL, T47D and WiDR Haryoto Haryoto; Muhtadi Muhtadi; Peni Indrayudha; Tanti Azizah; Andi Suhendi
Jurnal Penelitian Saintek Vol 18, No 2: Oktober 2013
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (965.482 KB) | DOI: 10.21831/jps.v18i2.2137

Abstract

Cynometra ramifolia Linn is known as Sala plant. This plant, especially in Solo City categorized as rare plant and traditionaly used to cure uric acid, diabetes, hypertension, and others pain. It is interesting to know the other activities by done the test for human cancer cell-lines. This research objective to identify the cytotoxicity effects of ethanol extract from C.ramiflora the stem bark and leaves against T47D, HeLa and WiDr cancer cell lines. The cytotoxicity test of ethanol extract from C.ramiflora the stem bark and leaves determined by MTT assay by calculating the level of IC50  which was based on the percentage of the cell death following the 24 hours incubation with the extract. The results  showed that ethanol extract of stem bark has cytotoxic effect to HeLa, T47D and WiDr cell-lines with the IC50  of 1000; 0,90 and 6,29 g/mL respectively. The leave cytotoxic effect to HeLa, T47D and WiDr cell-lines with the IC50 of 1,92;  6,37 and 0,41 g/mL respectively. This research indicated that the ethanol extract isolated from C. Ramiflora leaves a selective cytotoxicity effect to WiDr cell line. 
Identifikasi Isolat Ekstrak Etanol Kulit Batang Rhizophora apiculata Blume dan Rhizophora mucronata Lam Serta Sitotoksisitasnya Terhadap Sel MCF-7 dan T47D Joko Kristianto; Haryoto Haryoto; Peni Indrayudha
Pharmacon: Jurnal Farmasi Indonesia Vol 18, No 1 (2021)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v18i01.12252

Abstract

Mangrove plants as traditional medicine have long been used by the society for the therapy of anticancer diseases. So far, the potency of mangrove plants as anticancer has not been studied intensively. The methanol extract of R. mucronata stem bark has cytotoxic activity on myeloma cells. This study aims to determine the isolates of the ethyl acetate fraction of the methanol extract of the stem bark of R. mucronata and R. apiculata, and to examine the potency of cytotoxic activity against MCF-7 and T47D cells. The powder of the stem bark of R. mucronata and R. apiculata were extracted with methanol by maceration, and then the methanol extract was fractionated consecutively using n-hexane, ethyl acetate, and methanol. Ethyl acetate fraction was then purified by column chromatography with a diameter of 2 cm and a height of 30 cm with a static phase in the form of silica gel 60 (0.063-0.2 mm) Merck 7734 and eluent n-hexane:ethyl acetate (6: 4, 5: 5, 6: 4, 7: 3, 8: 2, 9: 1) and n-hexane: methanol (9,5: 0,5) 10 ml each. The eluates that produce the same number and pattern of stains were combined into one isolate. Then the eluate was evaporated at room temperature. Then the purification results of the extract R. mucronata were isolated using preparative TLC with 0.25 mm GF254 silica gel as static phase and n-hexane mobile phase: ethyl acetate (6: 4), while the apiculata extract with n-hexane mobile phase: ethyl acetate (5: 5). The results of the stain separation of the compound were scraped off and separated from the static phase using a solvent. The purity of isolates compound was examined using TLC in the static phase of silica gel GF254 0.25 mm. The qualitative test results of the ethyl acetate fraction of R. apiculata stem bark with an NMR spectrophotometer showed a composition of cis-3- (3,5-dihydroxyphenyl) acrylic acid, while the ethyl acetate fraction of R. mucronata stem bark contained n-hexan-3-ol compounds. The cytotoxicity test of ethanol extract, n-hexane fraction, ethyl acetate fraction and ethanol extract was carried out on MCF-7 and T47D cells using the MTT method. The results proved that the ethanol extract, n-hexane fraction, ethyl acetate fraction and ethanol fraction from the ethanol extract of R. mucronata and R. apiculata stem bark, based on cytotoxic activity, were inactive against MCF-7 and T47D cells.
Aktivitas Sitotoksik Ekstrak Etanol, Fraksi Etanol-Air, Etil Asetat serta N-Heksana Buah Pare (Momordica charantia) pada Sel MCF-7 secara In-Vitro Adhe Retnantya Pamungkas; Peni Indrayudha
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 2 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i2.9049

Abstract

Bitter melon is one of fruit that have pharmacological effects such as an anticancer which potentially active on breast cancer cell MCF-7. The aim of this study was to compare the potential cytotoxic activity between ethanol extract of pare and the results of ethanol, ethyl acetate and n-hexane fraction on MCF-7 breast cancer cells and to determine the class of compounds contained in each sample. Pare’s powder was extracted with maceration method in 80% ethanol solvent and then continue to fractionated in 96% ethanol, ethyl acetate and hexane solvent. Extract and fractions were continue to cytotoxic assay using the MTT assay method. Cytotoxic test results showed that the ethanolic extract had no potential cytotoxic activity. Ethyl acetate fraction with the highest concentration 16 µg/mL has the highest potential inhibition 43,87% on MCF-7 cells population. Extract and fractions than continue to phytochemical screening with Thin Layer Chromatography (TLC) method. TLC detection showed that n-hexane fraction contained more compound groups than ethanolic extract, ethanolic-water fraction and ethyl acetate fraction.
Aktivitas Sitotoksik Ekstrak Etanol, Fraksi Etanol, Etil-Asetat, dan Heksana Kulit Jeruk Purut (Citrus hystrix DC.) terhadap Sel Kanker Payudara T47D Isya Isma Nabilla; Peni Indrayudha
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8173

Abstract

Kaffir lime peels (Citrus hystric DC.) have been reported that it has activity on HeLa cell. The purpose of this study was to determine the cytotoxic activity of ethanol extract, ethanolic, ethyl acetate, and hexane fraction of kaffir lime peels to T47D breast cancer cells and to identify the compounds of kaffir lime peels (Citus hystrix DC.). Extraction was done by maceration using 96% ethanol. The results of thick extracts were fractionated by the liquid-liquid partition method using solvent with different polarity, namely hexane, ethyl acetate, and ethanol. Cytotoxic activity tests were carried out using the MTT assay. Identification of compounds were carried out by Thin Layer Chromatography (TLC) method with silica gel GF 254 as a stationary phase. The mobile phase used was hexane:ethylacetate (6:4) for ethanol extract, ethyl acetate and hexane fractions, and hexane:ethylacetate (4:6) for ethanol fractions. IC50 ethanol extract, ethyl acetate and hexane fractions obtained were 268.49, 286.82, and 136.27 mg/mL respectively. Ethanol extract, ethyl acetate, and hexane fractions contain alkaloid, flavonoid, terpenoid and phenolic compounds, while the ethanol fraction contains only flavonoid compound. Cytotoxic results of ethanol extract, ethanol, ethyl acetate, and hexane fraction of kaffir lime peels did not active against T47D breast cancer cells.
Cytotoxic Assay of Semipolar Fraction Of Ethanolic Extract From Sugar Apple (Annona Squamosa L.) Stem Bark on T47D Cells Cita Hanif Muflihah; Haryoto Haryoto; Peni Indrayudha
Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 2 (2020)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v17i2.12268

Abstract

Previous research has shown that some compounds in leaves and seeds of sugar apple have a cytotoxic activity. The aim of this research was to determine the cytotoxicity of semipolar fraction of ethanolic extract from sugar apple stem bark (Annona squamosa L.) on T47D cancer cells. The semipolar fraction of ethanolic extract from sugar apple stem bark was collected by fractionation using Vacuum Liquid Chromatography (VLC) with hexane:ethyl acetic (9:1, 8:2, 7:3, and  6:4)  as  mobile  phase.  Cytotoxicity from the fractions of five different concentration namely; 25, 50, 100, 150, and 250, µg/mL was measured by MTT assay. The potency of the cytotoxicity was defined by the ability of the fraction to inhibit the growth of T47D cells indicated by the value of IC50. Qualitative analysis of contained compounds in the fraction was done by Thin Layer Chromatography (TLC) method using silica gel F 254 as a stationary phase and hexane:ethyl acetic (7:3) as a mobile phase. UV 254 and 366 nm lamp also Dragendorff, citroboric, and FeCl3 spray reagents were used to visualize the spots of the secondary metabolites. The result proved that the semipolar fraction of ethanolic extract from sugar apple stem bark showed potential cytotoxicity on T47D cancer cells with IC50value of 70,77 µg/mL. Qualitative analysis showed that the fraction contained flavonoids and alkaloids which is presumably responsible for its cytotoxic activity. 
Co-Authors . MUHTADI . Muhtadi . Sismindari abdul, ahwan Adhe Retnantya Pamungkas Ahmad Farras Musyayaf Andi Suhendi Andika Andika Anita Sukmawati Anna Fitriawati Arini Nur Yunia Puspitaningrum Rahmwati Aryani, Diya Cita Hanif Muflihah Dedi Hanwar Dewi Rahmawati DEWI RAHMAWATI Em Sutrisna Firstca Aulia Rachma Haryoto Haryoto Haryoto Haryoto Haryoto Hidayah Kurniawati Ika Trisharyanti Dian Kusumowati Isya Isma Nabilla Joko Kristianto Muhammad Ajmal Shah Muhtadi Muhtadi Nabila, Devina Talitha Nabilla, Isya Isma Nastiti Wijayanti Nissa Ainni, Ayu Nova Lovena, Tri Nur Chasanah Nuri Utami Nursalinda Kusumawati Nurul Fadel, M. Pamungkas, Adhe Retnantya Pangesti, Adel Hening Prayitno, Imam Purwaningsih - Putri Agustina Rima Munawaroh Rossida , Salsabilla Aulya Saroyobudiyono, Haryoto Susi Iravati Tanti Azizah Tanti Azizah Sujono Tri Oktarina Tri Yulianti Tri Yulianti, Tri Wijaya, Abdul Rosyid Thoyib Wijaya, Abdul Rosyid Thoyib Wijianto, Diski Wahyu Yanuar, Rizqi Fitria Zakiyatul Fadhilah