Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

3.883

P-Index

This Author published in this journals

All Journal Journal of Food and Pharmaceutical Science PROSIDING SEMINAR NASIONAL Jurnal Ilmiah Farmasi Journal of Mathematical and Fundamental Sciences Jurnal Riset Kimia JPS Unimus Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Unnes Science Education Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Indonesian Journal of Chemistry JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Ilmu Kefarmasian Indonesia Jurnal Pengabdian Kesehatan Masyarakat Jurnal Farmasi Sains dan Praktis Prosiding Seminar Nasional Universitas Ma Chung Jurnal Manusia dan Lingkungan Journal of Food and Pharmaceutical Sciences Journal of Pharmascience Journal of Pharmaceutical Sciences and Community

Enade Perdana Istyastono

Department Of Pharmacy, Faculty Of Medicine And Health Sciences, Atma Jaya Catholic University Of Indonesia, Jalan Pluit Raya 2, Jakarta 14440, Indonesia

Author-ID : 290147

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

Published : 77 Documents Claim Missing Document

Claim Missing Document

Articles

4 5 6 7 8

Development of a Graphical User Interface Application to Identify Marginal and Potent Ligands for Estrogen Receptor Alpha Nunung Yuniarti; Sudi Mungkasi; Sri Hartati Yuliani; Enade Perdana Istyastono
Indonesian Journal of Chemistry Vol 19, No 2 (2019)
Publisher : Universitas Gadjah Mada

Employing ensemble Protein-Ligand Interaction Fingerprints (ensPLIF) as descriptors in post retrospective Structure-Based Virtual Screening (SBVS) campaigns Quantitative Structure-Activity Relationship (QSAR) analysis has been proven to significantly increase the predictive ability in the identification of potent ligands for estrogen receptor alpha (ERα). In the research presented in this article, similar approaches have been performed to construct and retrospectively validate an SBVS protocol to identify marginal ligands for ERα. Based on both validated SBVS protocols, a graphical-user-interface (GUI) application to identify if a compound is a non-, moderate or potent ligand for ERα was developed. The GUI application was subsequently used to virtually screen genistin, genistein, daidzin, and daidzein, followed by in vitro test employing a cytotoxic assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method.

Structure-Based Design and Molecular Dynamics Simulations of Pentapeptide AEYTR as a Potential Acetylcholinesterase Inhibitor Vivitri Dewi Prasasty; Enade Perdana Istyastono
Indonesian Journal of Chemistry Vol 20, No 4 (2020)
Publisher : Universitas Gadjah Mada

Structure-based virtual screening protocol to identify potent acetylcholinesterase inhibitors was retrospectively validated. The protocol could be employed to examine the potential of designed compounds as novel acetylcholinesterase inhibitors. In a research project designing short peptides as acetylcholinesterase inhibitors, peptide AEYTR emerged as one of the potential inhibitors. This article presents the design of AEYTR assisted by the validated protocol and guided by literature reviews followed by molecular dynamics studies to examine the interactions of the pentapeptide in the binding pocket of the acetylcholinesterase enzyme. The molecular dynamics simulations were performed using YASARA Structure in Google Cloud Platform. The peptide AEYTR was identified in silico as a potent acetylcholinesterase inhibitor with the average free energy of binding (DG) of -19.138 kcal/mol.

Docking-Guided 3D-QSAR Studies of 4-Aminoquinoline-1,3,5-triazines as Inhibitors for Plasmodium falciparum Dihydrofolate Reductase Radite Yogaswara; Maria Ludya Pulung; Sri Hartati Yuliani; Enade Perdana Istyastono
Indonesian Journal of Chemistry Vol 20, No 6 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.50674

Mutations in Plasmodium falciparum dihydrofolate reductase (PfDHFR), together with other mutations, hinder malaria elimination in Southeast Asia due to multiple drug resistance. In this article, molecular docking-guided three-dimensional (3D) quantitative structure-activity relationship (QSAR) analysis of 4-aminoquinoline-1,3,5-triazines as inhibitors for the wild-type (WT) PfDHFR to identify the molecular determinants of the inhibitors binding are presented. Compounds 4-aminoquinoline-1,3,5-triazines were reported promising to be developed as the non-resistant drugs. The 3D-QSAR analysis resulted in the best model with the R2 and Q2 values of 0.881 and 0.773, respectively. By correlating the molecular interaction fields (MIFs) of the best model to the docking pose employed to guide the 3D-QSAR analysis, S108 residue of the WT-PfDHFR was unfortunately recognized as one of the molecular determinants. Since the S108 residue is one of the mutation points of the PfDHFR mutants, the subsequent design strategy should modify the morpholine moiety to avoid the interaction with the S108 residue of the WT-PfDHFR.

Computer-Aided Discovery of Pentapeptide AEYTR as a Potent Acetylcholinesterase Inhibitor Enade Perdana Istyastono; Vivitri Dewi Prasasty
Indonesian Journal of Chemistry Vol 21, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.55447

One of the key targets in the drug development for potential Alzheimer’s disease (AD) therapeutics is the search for acetylcholinesterase enzyme (AChE) inhibitors. Very recently, a pentapeptide AEYTR was reported as a potential inhibitor for AChE. The peptide was identified in a retrospectively validated virtual screening campaign, which was subsequently followed by 10 ns molecular dynamics (MD) simulations. The study aimed to characterize the structure and identify in vitro of AEYTR peptide as a potent acetylcholinesterase inhibitor. This article presents the structure characterization and the in vitro examination of the peptide as an AChE inhibitor, followed by MD simulations for 100 ns. The results show that the pentapeptide is a potent AChE inhibitor with an IC50 value in the picomolar range and stabilizes the enzyme during MD simulations.

Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection Michael Raharja Gani; Enade Perdana Istyastono
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.61462

We developed a method for determining the caffeic acid in spent coffee grounds. The spent coffee ground solution was prepared by blending 3 g spent coffee grounds with 60 mL ethanol/water (40/60 v/v) for 2 h on a hot plate magnetic stirrer (60 °C, 350 rpm). The mixture was filtered and the filtrate was concentrated under vacuum (60 °C) to 5 mL. The method employed a reversed-phase high-performance liquid chromatography with a UV detector. We used a Phenomenex Luna column (250 × 4.6 mm; i.d., 5 µm) under isocratic elution, and the mobile phase was acetonitrile-methanol-aqueous formic acid (10:10:80 v/v), with a flow rate of 0.9 mL/min. Analysis was performed at 324 nm. The column temperature was set at 27 °C temperature. The results showed that this method was selective for quantifying the caffeic acid in spent coffee grounds with good linearity in the range of 1.31–17.07 μg/mL. The detection and quantitation limits were 0.28 and 0.84 μg/mL, respectively. The mean intraday and interday recoveries were 83.80–95.17% and 82.16–97.40%, respectively. Intraday and interday precision expressed as the relative standard deviation (RSD) were below 7.3%. There was 0.17% ± 0.006 w/w caffeic acid in the spent coffee grounds (RSD = 3.63%, n = 3).

Identifikasi Determinan Molekul Interaksi STK630921 pada Interleukin-17A Fransiscus Deddy Riandono; Enade Perdana Istyastono
Jurnal Sains Farmasi & Klinis Vol 9, No 1 (2022): J Sains Farm Klin 9(1), April 2022
Publisher : Fakultas Farmasi Universitas Andalas

Interleukin-17A (IL-17A) merupakan sitokin pro-inflamasi yang terlibat dalam patogenesis beberapa penyakit, antara lain psoriasis, rheumatoid arthritis, kanker, diabetes dan penyakit ginjal stadium akhir. Peningkatan kadar serum IL-17A memberikan petunjuk adanya keterkaitan antara IL-17A pada kejadian nefropati diabetik. Keterkaitan ini juga diperjelas dengan temuan bahwa penghambatan aktivitas IL-17A dapat menurunkan albuminuria, cedera ginjal dan menunda perkembangan nefropati diabetik. Peranan IL-17A ini menjadikannya sebagai pilihan target potensial terapi nefropati diabetik yang sejauh ini bertumpu pada pengendalian optimal sistem renin angiotensin. Penelitian ini bertujuan mengidentifikasi determinan molekul interaksi STK630921 pada IL-17A. Penelitian dilakukan dengan penambatan molekul STK630921 pada protein IL-17A, dilanjutkan dengan simulasi dinamika molekul selama 15 ns. Identifikasi determinan molekul dilakukan menggunakan bantuan perangkat lunak PyPLIF-HIPPOS terhadap hasil simulasi dinamika molekul. Penelitian ini berhasil mengidentifikasikan asam-asam amino yang berperan penting yaitu His29, Trp67, Asn27, Lys114 dan Glu95 dengan interaksi aromatik dan interaksi hidrogen sebagai jenis interaksi yang berperan pada aktivitas STK630921 pada struktur protein 4HR9.

ROOT CAUSE ANALYSIS (RCA) OF E-CATALOGUE PROCUREMENT OF MEDICINE USING E-PURCHASING IN PUBLIC HEALTH CENTER IN KLATEN REGENCY OF CENTRAL JAVA Liana Roh Widiyani; Enade Perdana Istyastono; Nunung Priyatni
Jurnal Farmasi Sains dan Praktis Vol 9 No 2 (May-August 2023)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v9i2.7970

E-catalogue procurement of medicine is a method of electronic procurement using a particular website developed by the Government to increase the effectiveness, efficiency, and transparency of medicine procurement. In Klaten Regency, e-catalogue procurement of medicine in the Public Health Center (PHC) experiences obstacles. The study aimed to evaluate performance of e-catalogue procurement of medicine, analyze the root problem and formulate strategies to improve the performance based on the root problems. This descriptive study involved seven PHCs with data on medicine procurement in 2021. Quantitative data analysis was conducted by evaluating six performance indicators of medicine procurement. Qualitative data analysis was conducted by interviewing 17 respondents, analyzing root problems, and formulating improvement strategies The study showed that all PHCs had good medicine availability at 87.71% average, but low realization of e-catalogue procurement occurred in two PHCs. Lead time beyond the contract period occurred in two PHCs. All PHCs experienced low suitability of procurement funds and percentage of procurement through e-catalogue. Percentage of budget allocation for medicine procurement varied between 1.59% to 7.29%. Analysis of the root problem showed obstacles in human resources, mechanism of procurement, policies, system, budget, and environment aspect. It was concluded that the obstacles rooted on the lack of team role and synergy of PHC, District Pharmacy Unit, and District Health Office according to their authority, the lack of pharmaceutical industry and distributor commitments in the provision of e-catalogue medicine for PHC, and also the urgent needs of system and regulatory improvement by the Government.

Molecular Dynamics Study of Human MTHFR Wild-Type and A222V Mutant-Type: A Computational Approach Michael Resta Surya Yanuar; Dita Maria Virginia; Enade Perdana Istyastono
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 20, No 1 (2023)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.005727

Methylenetetrahydrofolate reductase (MTHFR) is an essential enzyme for the homeostasis and metabolism of intracellular folate. However, it is known that one of the commonly found MTHFR gene SNPs (A222V) causes a decrease in activity which decreases folate levels and increases the accumulation of homocysteine in the blood. The purpose of this study was to investigate the molecular dynamics of MTHFR wild-type protein and MTHFR A222V mutant protein in silico. After some preparations, the crystal structure of MTHFR with code 6FCX obtained from the Protein Data Bank was used as the input file for molecular dynamics (MD) simulations. YASARA-Structure was employed for performing 50 ns production run of MD simulations. The deviation of the root-mean-squared deviation value of the backbone atoms (∆RMSDBb) of MTHFR wild-type is consistently less than 2 Å from the beginning of the MD simulations production runs. On the other hand, there is a sudden spike of ∆RMSDBb of MTHFR A222V from 15.01 to 20.00 ns of 2.221 Å. According to the analysis of ∆RMSDBb, MTHFR wild-type protein is considered stable and the MTHFR A222V mutant protein is considered unstable which may lead to various clinical problems for the person possessing this mutation.

KUALITAS AIR SUNGAI GAJAH WONG DITINJAU DARI PENGHAMBATAN ENZIM ASETILKOLINESTERASE (Water Quality Assessment of Gajah Wong River Based on Inhibition of Acetylcholinesterase Activity) Florentinus Dika Octa Riswanto; Maywan Hariono; Gatot Nugroho Susanto; Ignatius Yulius Kristio Budiasmoro; Enade Perdana Istyastono
Jurnal Manusia dan Lingkungan Vol 24, No 2 (2017): Mei
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.23046

ASBTRAKSungai Gajah Wong, salah satu sungai di kota Yogyakarta, dikhawatirkan terpapar pencemaran air akibat pembuangan limbah rumah tangga, industri, rumah sakit maupun hotel yang masuk ke dalam alirannya. Hal ini menyebabkan penurunan kualitas air sungai dan menimbulkan dampak negatif bagi makhluk hidup yang memanfaatkannya. Pencemaran air sungai tersebut dapat diamati secara biokimiawi berdasarkan uji aktivitas penghambatan enzim asetilkolinesterase (AChE) oleh cemaran pestisida organofosfat dan karbamat. Uji biokimiawi dalam analisis kualitas air terhadap penghambatan AChE dapat dilakukan secara kolorimetrik menurut metode Ellman. Hasil reaksi ini dapat diamati secara melalui pembentukan senyawa berwarna dan serapannya dapat diukur pada panjang gelombang visibel. Penentuan lokasi sampling pada penelitian didasarkan pada pertimbangan sebaran muatan polusi/diffuse pollution loads consideration. Ditetapkan enam lokasi pengambilan sampel air sungai yaitu GW-1, GW-2, GW-3, GW-4, GW-5, dan GW-6 berturut-turut dari utara ke selatan. Dari keenam lokasi, GW-6 sebagai titik paling selatan dalam jalur pengambilan sampel menunjukkan penghambatan aktivitas AChE yang paling tinggi ditunjukkan dengan aktivitas AChE yang rendah. ASBTRACTGajah Wong River, one of the rivers in Yogyakarta city, was threatened by contamination of water pollutant due to the disposal waste of households, industrials, hospitals and hotels into its stream. The pollutions might cause the decreasing of water quality and raised the negative impact to living creatures that exploit it. River pollutions can be observed biochemically based on inhibition of acetylcholinesterase (AChE) enzyme activity by organophosphate and carbamate pesticide contamination. Biochemical assay in the analysis of water quality could be done according to the colorimetric of Ellman’s method. The results of this reaction can be observed by measuring the formation of colored compounds at visible wavelengths. Determination of the sampling location in this research was performed based on diffuse pollution loads consideration. Six sampling sites was defined and named as GW-1, GW 2, GW 3, GW 4, GW-5, and GW-6, respectively in a row from north to south. As the results, water from GW-6 as the southernmost site in the sampling sites showed the highest inhibition of AChE activity.

Molecular Dynamics Simulation of Serotonin Transport Protein Complex with 6-Hydroxy-1-Methyl-1,2,3,4-Tetrahydro-β-Carboline Ligand from Chocolate (Theobroma cacao L.) Isolate Farach Khanifah; Enade Perdana Istyastono
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 20, No 2 (2023)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.005939

6-hydroxy-1-methyl1,2,3,4-tetrahydro-β-carboline (6OHMTHβC) is a chocolate derivate that has antidepressant potency. It can increase dopamine and serotonin secretion, which leads to mood improvement. This method was carried out using computational molecular docking simulations (in silico). The research design was computational-based exploratory descriptive. The results of molecular docking showed the lowest energy score and the backbone RMSD value ≤2Å. The procedure performed was 6AWP receptor docking without ligand, with native ligand (6OHMTHβC), and with reference ligand (fluvoxamine). This study also performed molecular docking simulations of 6OHMTHβC towards 6AWP to find compounds in the receptor binding pocket. This study also performed dynamics simulations and identified the molecular determinants using PyPLIF-HIPPOS and YASARA Structure software 20.1.24.10 with the Windows 10 operating system. This study succeeded in determining the stability of the dynamics simulation of the serotonin transport protein complex with the reference ligand 6OHMTHβC for 50 ns, and this result corresponds to the RMSD value and binding energy. The determination of binding energy (BE) was calculated from the BE calculation available at YASARA and Ubuntu. The binding energy value of the original ligand was -11.6590 kJ/mol, and the reference ligand was -83880 kJ/mol. The highest RMSD value of the original ligand was 1.39292Å, while the RMSD value of the reference ligand was 1.71072Å. The essential amino acid carried out was 438Ser with hydrogen bond interactions, so 6OHMTHβC was considered a competent antidepressant candidate.

Co-Authors ., Jumina Abdul Mutholib Aditya Asyhar Agnes Mutiara Kurniawan Agnes Nora Iska Harnita Agnes Nora Iska Harnita Agung Endro Nugroho Agustina Setiawati Akhmad Darmawan Andreas Asdi Utama ARGUN WIDARSA ARIEF NURROCHMAD As'ari Nawawi Berlina, Violita Yessi Bonifacius Ivan Wiranata Bonifacius Ivan Wiranata Bonifacius Ivan Wiranata Chrisanti, Marcella Widya Chrisologus Ivan Prasetyo David Chandra Putra Dewi Setyaningsih Dina Christin Ayuning Putri Dita Maria Virginia Eko Yuliyanto Eko Yuliyanto, Eko Esti Mumpuni Evy Fenny Veronica Fajar Agung Dwi Hartanto Fajar Ira Juwita Farach Khanifah Felicia Felicia Felicia Fraulein Setiawan Fitria Fatichatul Hidayah Fitria Fatichatul Hidayah Fitria Fatichatul Hidayah Florentinus D.O. Riswanto Florentinus Dika Octa Riswanto Florentinus Dika Octa Riswanto Florentinus Dika Octa Riswanto Florentinus Dika Octa Riswanto Fransiscus Deddy Riandono Fransiska Kurniawan Gani, Michael Gatot Nugroho Susanto Gerry Nugraha Glory Ivana Ardine Harno Dwi Pranowo Harno Dwi Pranowo Harno Dwi Pranowo Herluin Sekar Krisantia I Gusti Ayu Intan Puspita Dewi Ignasius Widya Parahita Putranto Ignatius Yulius Kristio Budiasmoro Iqmal Tahir Iwan J. P. de Esch Jeffry Julianus Julyus Jason Mark Jutti Levita Liana Roh Widiyani Liliana Liliana Lukman Hakim Marchaban Marchaban Marchaban Marchaban Marchaban Marchaban Maria Ludya Pulung Maywan Hariono Maywan Hariono Michael Raharja Gani Michael Raharja Gani Michael Raharja Gani Michael Resta Surya Yanuar Muhamad Hanafi Muhammad Radifar Muhammad Radifar MUHAMMAD RADIFAR Muhammad Radifar Natalia Kristina Nugraha, Gerry Nunung Priyatni Nunung Yuniarti Nunung Yuniarti Nunung Yuniarti Nunung Yuniarti Nunung Yuniarti Nunung Yuniarti Nunung Yuniarti Octa Riswanto , Florentinus Dika OISAN OISAN Oka, Mitsue Perdana Adhi Nugroho Pramono, Zita Dhirani Primana, Mala Hikmawan Pudjono Pudjono Radite Yogaswara Rekso, Putri Ayu Dharmo reza eka putra Riandono, Fransiscus Deddy Rini Dwiastuti Rizna Triana Dewi Roy Gunawan Wicaksono Rr. Sri Untari Siwi S.M.P Sardjiman Sardjiman Slamet Ibrahim - Sri Hartati Yuliani Sri Noegrohati Sri Noegrohati St. Layli Prasojo Stefanus Layli Prasojo Stephanus Satria Wira Waskitha Sudibyo Martono Sudibyo Martono Sudibyo Martono Sudibyo Martono Sudibyo Martono Supardjan . Supardjan . Supardjan A. Margono Supardjan A. Margono Supardjan A.M Supardjan A.M. Supardjan AM Tasia Amelia Titien Siwi Hartayu Umar Anggara Jenie Vivitri Dewi Prasasty Waskitha, Stephanus Satria Wira Widayati, Aris Windah, Axl Laurens Lukas Wira Waskitha, Stephanus Satria Wiranata, Bonifacius Ivan YANTI SUSILAWATI Yohanes Eko Adi Prasetyanto Yosef Wijoyo Yosef Wijoyo Yosef Wijoyo, Yosef Yosi Bayu Murti Yulia Anita YUMI RAHMADANI Yuniarti, Nunung Zullies Ikawati Zullies Ikawati

Title

Found 77 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 77 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 77 Documents
Search