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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Animal Production : Indonesian Journal of Animal Production Jurnal Pengabdian Pada Masyarakat Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) IGKOJEI: Jurnal Pengabdian Masyarakat Jurnal Abdimas Bina Bangsa Jurnal Kedokteran Hewan

Irkham Widiyono

Dept Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna No. 2, Kampus UGM, Karangmalang, Yogyakarta, Indonesia 55281

Author-ID : 2938381

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Keterkaitan Panhisterektomi dan Suplemen 1,25- Dihidroksivitamin D3 dengan Risiko Urolitiasis pada Tikus (CORRELATION BETWEEN PANHISTERCTOMY AND 1.25-DIHYDROXYVITAMIN D3 SUPPLEMENTATION ON RATS UROLITHIASIS RISK) Hartiningsih .; Devita Anggraeni; Irkham Widiyono; Hastari Wuryastuti
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objective of this research was to study the correlation of panhisterectomy and supplement 1.25-dihydroxyvitamin D3 on urolithiasis risk in Wistar rats. Twenty female Wistar rats at 8 weeks of age, weredivided into four groups (control fed standard diet, control fed standard diet+1,25-dihydroxyvitamin D3 supplement, panhisterectomy fed standard diet and panhisterectomy fed standard diet +1,25-dihydroxyvitamin D3 supplement). Eleven weeks after treatment, each of rats was placed into individualmetabolic cage for balance study for a week. From day 4 to 11 of the balance study, every morning theremaining food, feces, and urine were collected and recorded for calcium (Ca) analysis. At the end ofbalance study, blood samples were taken from canthus retroorbitalis medialis for estrogen analysis. Theresults showed urinary and fecal Ca excretions were not significantly different compared to the controlgroup. Calcium consumption was significantly higher (P<0.05) in panhisterectomized rats compared withthose in control rats. While, estrogen in panhisterectomized group was not significantly different to thosein control rats. Calcium urinary and Ca consumption in rats consuming 1,25-dihydroxyvitamin D3 supplement were significantly higher (P<0.05) compared with those in without 1,25-dihydroxyvitamin D3 supplementation, but Ca excretion in feses was not significantly different. Estrogen in rats consuming1.25-dihydroxyvitamin D3 supplement was significantly lower (P<0.05) compared with the rats that without1,25-dihydroxyvitamin D3 supplemention. It can be concluded that panhisterectomy does not seem to affecturolithiasis risk, while 1,25-dihydroxyvitamin D3 supplement may affect urolithiasis risk. There is likelyno association between panhisterectomy and 1.25-dihydroxyvitamin D3 supplementation on urolithiasisrisk in Wistar rats.

Respons Stres Sapi Bali Jantan pada Proses Pengangkutan dan Pemuatan ke Atas Kapal Ternak (STRESS RESPONSE OF BALI BULLS DURING TRANSPORTATION AND LOADING PROCCESS ONTO THE CATTLE SHIP) Sri Widayati; Puji Astuti; Irkham Widiyono
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Transportation is needed for livestock distribution from production centers to consumption centers. This might potentially result in a stressful condition for the cattle. The study was aimed to observe the stress response of Bali bulls during the transport and loading proccess onto the ship. Six adult clinically healthy Bali bulls were used in this study. The animals were housed in pen at the animal quarantine installation, given dried straw and drinking water ad libitum since three days before the study. The animals were loaded on the truck in 30 minutes, transported to the harbor in 15 minutes, and loaded on to the ship in 30 minutes. On the ship the animals were kept in a pen bedded with straw and given dried straw and drinking water ad libitum for 19 hours. Venous blood samples were collected from jugular vein during the rest in pen before loading on to the truck, after loading on the ship, and about 19 hours after loading and resting in the ship pen. Serum was separated by centrifugation and stored at -20oC for cortisol analysis. The concentrations of cortisol was analyzed by using enzyme linked immunosorbent assay (ELISA). The results showed that the average of serum cortisol concentration of Bali bulls was at the level of 33.81±28.52 ng/mL in the rest period at the quarantine installation, increased significantly to the level of 138.41±102.57 ng/mL after loading onto the ship (P<0.05), and decreased again to the level of 41.54±43.47 ng/mL after 19 hours of resting period in the pen on the ship (P>0.05). It can be concluded that Bali bulls may suffer from stress during transport and loading proccess which can be recovered after resting in the ship pen.

Penentuan Efektifitas Oksitetrasiklin Melalui Parameter Farmakokinetik/farmakodinamik pada Plasma dan Jaringan Ayam Broiler Agustina Dwi Wijayanti; Lukman Hakim; Irkham Widiyono; Tatang Irianti
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was conducted to determine the effectiveness of oxytetracycline on the basis of itspharmacokinetic/pharmacodynamic (PK/PD) parameters in the plasma and tissues of broiler following a50 mg/kg single dose of intravenous administration. The male broiler were injected with oxytetracycline attarsal medial venous. The blood, liver, kidney and abdominal muscle were collected at 14 points of timesamplings. Blood was centrifuged to obtain plasma and all tissues were extracted with Mc Ilvine buffer.Drug concentration in samples was determined by High Performance Liquid Chromatography (HPLC)using AOAC standard reference for tetracycline. The pharmacokinetic parameters of oxytetracycline werecalculated using non compartment method. The results were as follows. For plasma: the AUC was 790,615,5 ?g/mL minute , Clearance was 63,242 mL/min/kg bb, Tmax/Cmax was 1 minute/0,7 ?g/mL, T1/2elimination was 6,4, hours, Vd was 37 L/kg. For liver ; AUC was 36418,89, minute , Tmax/Cmax was 16hours/17,15 ?g/g, and T1/2 elimination was 24,5, hours. For kidney; AUC6808,41 ?g/g minute , Tmax/Cmax 1 minute/16,73 ?g/g, , T1/2 elimination was 11,55 hours. For muscle; AUC was 3502 ?g/g minute,Tmax/Cmax was 1 minute/2,58 ?g/g, T1/2 elimination was 167,39 hours. The ratio of AUC/MIC inplasma and tissues appeared to a good pharmacokinetic/pharmacodynamic parameter to determine theeffectiveness of oxytetracycline.

Pengaruh Panhisterektomi dan Konsumsi Suplemen 1,25-Dihidroksivitamin D3 Selama 1,5 Bulan terhadap Retensi Kalsium pada Tikus Wistar Hartiningsih -; Devita Anggraini; Irkham Widiyono
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objective of the research was to study the effect of panhisterectomy and 1,25-dihydroxyvitamin D3supplement consumption for 1.5 months on Ca retention of Wistar rats fed with casein. Twenty female ofWistar rats, 8 weeks of age were randomly divided into four groups (normal group N and NK,panhisterectomized group H and HK) in wich group consisted of 5 rats. Group N and H rats were fed witha standard diet, while group NK and HK rats were fed with a standard diet+1,25-dihydroxyvitamin D3supplement. At 19 weeks of age, they were placed into individual metabolic cages for balance studies.From day 7 to 11 of the balance studies, daily unconsumed food, urine, and feces were collected andrecorded for Ca analyses. The research results showed that Ca comsumption and fecal Ca excretion weresignificantly higher (P<0.05) in panhisterectomized rats consuming 1,25-dihydroxyvitamin D3 supplement,as compared with the normal rats consuming 1,25-dihydroxyvitamin D3 supplement. Urinary Ca excretionand Ca retention in panhisterectomized rats were not significantly different those of normal rats. WhileCa consumption and fecal Ca excretion were significantly reduced (P<0.05) in normal rats consuming 1,25-dihydroxyvitamin D3 supplement but urinary Ca excretion and Ca retention in normal rats consuming1,25-dihydroxyvitamin D3 supplement were not significantly different compare with normal rats notconsuming 1,25-dihydroxyvitamin D3 supplement. It is evident that panhisterectomy and 1,25-dihydroxyvitamin D3 supplement did not affect the calcium retention.

Kadar Estradiol dan Progesteron Serum, Tampilan Vulva dan Sitologi Apus Vagina Kambing Bligon Selama Siklus Birahi Irkham Widiyono; Prabowo Purwono Putro; Sarmin -; Pudji Astuti; Claude Mona Airin
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was carried out to determine the serum estradiol and progesterone concentration, vulvaappearance, and vaginal cytology during estrous cycle in bligon goat. Four female non pregnant bligongoats (Indonesian indigenous) were used in this research. They were clinically healthy and showed normalestrous cycle. Estrous state was examined by using biological method. When the animals showed acceptanceto be mounted by a buck, they were confirmed as the first day of estrous cycle (estrous phase). Collectionof blood sample, preparation of vaginal smears, and measurement of some physical genital parameterswere conducted by standard veterinary clinical methods at day 11, day between 3-5, day between 6-16, andday between 17-19 of estrous cycle. Enzyme linked immunosorbent assay (ELISA) was used to determineserum progesterone and estradiol concentrations. Mean difference of each parameter was analyzed byanalyses of variance (Anova), followed by Tuckey HSD. The serum estradiol concentration ranged between211.25-247.77 pg/ml. Peak of the estradiol was occurred at the first day of estrous phase, namely 247.77pg/ml and to decrease at day 3-16. The serum progesterone concentration was at the level of 0.21-0.70 ng/ml and showed a significant increase at day 6-16 (p<0.05). The specific physical genital changes (swellingvulva, reddish mucous membrane of vulva, and excretion of viscous transparent vaginal discharge) wereidentified as the animals were at the estrous phase. Composition of exfoliative vaginal cytology did notshow any specific and consistent changes throughout the estrous cycle. Superficial and intermediate cellswere very dominant (>60%) during estrous cycle. In conclusion, serum estradiol, progesterone, the vulvaappearance combined with sexual receptivity could be a good estrous predictor for female cycling bligongoat.

Respons Metabolik terhadap Pembatasan Asupan Pakan pada Kambing Peranakan Ettawa (METABOLIC RESPONSES TO FOOD RESTRICTION IN ETTAWA CROSSBRED GOATS) Irkham Widiyono; Sarmin .; Bambang Suwignyo
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The study aim of this was to evaluate the effects of food restriction on metabolic aspects (bloodchemistry parameters) in Ettawa crossbred goats. Four adult male and clinically healthy Ettawa crossbredgoats were used in this study. The animals were kept in individual cage for 12 weeks. Animal feed consistedof aerial part of peanut plant and concentrate at a ratio of 60:40. The experiment was devided into 2phases of feeding periods, fullfeeding (ad libitum feeding and restricted feeding. After the adaptationperiod for 4 weeks (week 1-4), the animals were fed ad libitum for 4 weeks (week 5-8), and then continuedwith 50% of the ad libitum feed intake during the following 4 weeks restricted feeding period (week 9-12).During the experiment, drinking water was supplied ad libitum. Body weight were examined and bloodsamples were collected at the end of the fullfeeding and restricted feeding periods for blood chemistryanalyses. Statistical differences were determined by paired t-Test at probability level of 0.05. This studyshowed that feed restriction up to about 50% of ad libitum (80% of maintenance) feed intake resulted inchanges of blood chemistry parameters. At the end of restricted feeding period, the elevated concentrationof serum inorganic phosphate, creatinine, blood urea nitrogen, and cholesterine were observed and reducedconcentration of glucose, calcium, and triglyceride were observed. However no change was observed on levelof arterial blood pH and the concentration of serum natrium, potassium, chloride, and protein. In conclusion,nutritional status has significant effect on blood chemistry parameters of Ettawa crossbred goats. Seruminorganic phosphate, calcium, creatinine, blood urea nitrogen, cholesterine, glucose, and triglycerideconcentration could provide the more reliable information for assessment of the nutritional status ofEttawa crossbred goat.

Pembangunan Infrastruktur Pertanian dan Sosial Dalam Rangka Mempersiapkan Selopamioro Mandiri Sejahtera Berbasis Potensi Lokal Sarmin Sarmin; Irkham Widiyono; Slamet Widiyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 1 (2016): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Tis program aims to develop agricultural infrastructure and social culture for Selopamioro empowering and prosperous based on local potential. Te priority of frst year programs were development of water reservoir in Kalidadap 2 and Srunggo, smart home fnishing in Srunggo 1, processed cashew as jam and shredded, promotion assistance in Bantul Expo event, branding and packaging innovation for cashew, to initiate government licensing, and develop standar operating procedure in processed cashew. All of programs as baseline to holistic development in Selopamioro based on local potential.

Pemberdayaan Peternak Marginal: Studi Kasus di Wilayah Banguntapan Bantul Irkham Widiyono; Sarmin Sarmin
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Over the recent years, villagers in the countryside havefaced the problem of limited resources for livestock and crop productions. Decreased land quality and agricultural land areawith respect to the increased need for housing landhas resulted in economic and environmental problems for livestock farmers in this area. Most livestock farmers have poor standard of education, limited ownership of land and livestock, and are not capable of managing the natural resources in sustainable ways. The present services for small livestock farmersare aimed to transform the unintegrated livestock and agricultural farming into a sustainable integrated system in the marginal community. Some efforts were conducted to improve knowledge and skills in zero waste system in livestock and agricultural farming by livestock farmers in marginal areas. The present community development programs were conducted using farmer-centered learning methods (participative learning) and mentoring by professionals on the implementation of sustainable integrated farming system (learning by practice). Furthermore, institutional development and cooperative networking with related parties were carried out. Results showed that the livestock farmerswere able to adopt integrated farming practices and the community was capable of independently producing organic fertilizer from livestock wastes and converting agricultural byproducts into animal feed. Both farmers and villagers have come together to cultivate fruit and vegetable plantations in vacant lands and back yards which were previously unused. The farmers’ group has successfully networked with the related partners to ensure sustainability of environmental conservation efforts and improvement of livelihood. In conclusion, active participation of farmersin the marginal area throughout the development process and mentored-practice are key factors for successful agricultural transformation in the marginal community. Implementation of the sustainable integrated farming system will ensure food security, environmental protection and safety, conservation of natural resources, and better quality of life.

Restriksi Pakan yang Diikuti dengan Refeeding Menurunan Level Fosfat Inorganik dan Kalsium pada Kambing Kacang Jantan Dewasa (FEED RESTRICTION FOLLOWED BY REFEEDING DECREASED THE INORGANIC PHOSPHATE AND CALCIUM LEVELS IN ADULT KACANG GOATS) Sarmin Sarmin; Irkham Widiyono; Pudji Astuti; Prabowo Purwono Putro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this research was to evaluate metabolism of inorganic phosphat (Pi) and calcium (Ca) in kacang goats subjected to 60-d feed restriction and 60-d full feeding (refeeding). Nine adult male goats were divided into three groups used in this study. Restrictions include 40% (group I), 50% (group II), and 60% (group III) of the ration of dry fodder. To obtain the baseline all animals from all groups were fullfeeding from the start of the study (day 0) to day 60 then followed by restriction stage on day 61 to day 120. The next stage is refeeding from day 121 to 180. Pi and Ca levels were analyzed on days 60, 120, and 180, respectively. The results showed that restriction followed by refeeding decreased Ca levels in all three groups and Pi of animals in groups II and III was below the baseline. The level of Pi reach above the baseline were seen only in animals in group I following restriction and refeeding, although there was a tendency to decrease after refeeding. Based on the results of the study it is concluded that the restriction followed by refeeding decreases the level of Pi and Ca in adult male kacang goats. ABSTRAK Tujuan penelitian ini adalah mengkaji metabolisme fosfat inorganik (Pi) dan kalsium (Ca) selama 60 hari restriksi (pembatasan) pakan yang diikuti dengan 60 hari pemberian pakan kembali secara fullfeeding (refeeding) pada kambing kacang jantan dewasa. Sembilan ekor kambing kacang jantan dewasa terbagi menjadi tiga kelompok digunakan dalam penelitian ini. Restriksi meliputi 40% (kelompok I), 50% (kelompok II), dan 60% (kelompok III) dari 3,5% bahan kering dari bobot badan. Tahap awal sebagai baseline dimulai dengan pemberian pakan fullfeeding pada hari ke-0 sampai hari ke-60 dilanjutkan dengan tahap restriksi dimulai pada hari ke-61 sampai hari ke-120. Tahap berikutnya adalah refeeding mulai hari ke-121 sampai dengan hari ke-180. Level Pi dan Ca dianalisis pada hari ke-60, 120, dan 180. Hasil penelitian menunjukkan bahwa restriksi yang diikuti refeeding menurunkan level Ca pada ketiga kelompok dan Pi kelompok II dan III di bawah baseline. Level Pi berada di atas baseline hanya pada kelompok I setelah restriksi dan refeeding, meskipun cenderung menurun setelah refeeding Berdasarkan hasil penelitian disimpulkan bahwa restriksi yang diikuti dengan refeeding menurunkan level Pi dan Ca pada kambing kacang jantan dewasa.

Respons Tulang Femur Tikus Ovariohisterektomi yang Mengkonsumsi Kasein dan Disuplementasi Calcitriol Selama 30 Minggu (THE RESPONSE OF BONE FEMUR OVARIOHISTERECTOMIZED RATS CONSUMING CASEIN AND CALCITRIOL SUPPLEMENTATION FOR 30 WEEKS) Hartiningsih Hartiningsih; Devita Anggraeni; Irkham Widiyono; Hastari Wuryastuty
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Calcitriol supplementation in ovariohisterectomized rat was known to decreased calcium retention.The objective of the research was to study the response of femur bone to calcitriol supplementation for 30weeks in ovariohisterectomized rats consuming casein. Twenty female Wistar rats at 8 weeks of age wererandomly divided into four groups (unovariohisterectomy without calcitriol supplementation (N),unovariohisterectomy with calcitriol supplementation (ND), ovariohisterectomy without calcitriolsupplementation (O) and ovariohisterectomy with calcitriol supplementation (OD) of five each. Thirtyweeks after surgery, femur was taken for histopathological and immunohistochemistry examination.Immunohistochemistry of distal femur metaphysis in group O and OD were revealed decreasing tartrateresistant alkaline phosphatase 5b (TRAP5b) in trabecular bone, which was located in bone marrow space,and also in trabecular speculum surface. Histopathological analysis of distal femur metaphysis in groupN and ND were showed normal structure, meanwhile, distal femur metaphysis in group O and OD wereshown some abonormalities, such as increased of bone marrow space, domination of adipocytes in the bonemarrow, and decreased of trabecular bone speculum in metaphysis. Based on the results, it was concludedthat femur bone of ovariohisterectomized rats fed casein with and without calcitriol 8ng/daysupplementation for 30 weeks were showed unbalanced between resorption and formation of bone whichwas domination by bone resorption.

Co-Authors . Hartiningsih Agustina Dwi Wijayanti Agustina Dwi Wijayanti Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi, Alfarisa Alfariza Nururozi Angeline Ganapragasam Aryanto Aryanto Asih Kurniawati Bambang Suwignyo Christin Melkianus Claude Mona Airin Corry Devita Anggraeni Devita Anggraeni Devita Anggraini Djarot Heru Santosa Eko Agus Suyono Elfina Okto Posmaida Damanik Fitriana, Ida Guntari Titik Mulyani Hartiningsih - Hartiningsih . Hartiningsih . Hartiningsih Hartiningsih Hary Purnamaningsih Hary Purnamaningsih Hastari Wuryastuti Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty I Gusti Bagus Wiksuana Ida Fitriana Ida Tjahajati Jhonson Allen Marbun Kelviano Muqit Laksono Trisnantoro Lukman Hakim Nareswari, Anggitya Novia Nur Aini Okid Parama Astirin Prabowo Purwono Putro Prabowo Purwono Putro Pudji Astuti PUDJI ASTUTI Puji Astuti Refika Melina Putri Rieska Indriani Rieska Indriani Rolando Sihombing Rusmi Hayati Rusmihayati Rusmihayati Sarmin - Sarmin . Sarmin Sarmin Sarmin Sarmin Slamet Rahardjo Slamet Raharjo Slamet Raharjo Slamet Raharjo Slamet Widiyanto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Widayati Suparni Setyowati Rahayu Tatang Irianti Tioner Purba Tridjoko Wisnu Murti Trini Susmiyati Ulil Amri Wijaya Ulil Amri Wijaya Wisnu Nurcahyo Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono, Yanuartono Yeo Suan Jiao Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi, Yuriadi

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