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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala Majalah Kedokteran Bandung AL KAUNIYAH e-Journal Pustaka Kesehatan Jurnal Sains dan Teknologi Althea Medical Journal Jurnal Kesehatan Andalas Research Journal of Life Science Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Health Notions Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Pengabdian Pada Masyarakat Warta Pengabdian Multidiciplinary Journal Al-sihah: The Public Health Science Journal Manuju : Malahayati Nursing Journal Majalah Kedokteran Andalas Jurnal Inovasi Teknik Kimia Jurnal ABDINUS : Jurnal Pengabdian Nusantara JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH AloHA International Journal of Health Advancement (AIJHA) Jurnal Kesehatan dr. Soebandi Strada Jurnal Ilmiah Kesehatan (SJIK)
Erma Sulistyaningsih
Department Of Parasitology, Faculty Of Medicine, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Samsul Arifin; Loeki Enggar Fitri; Hidayat Sujuti; Bagus Hermansyah; Agustina Tri Endharti; Niniek Burhan; Didi Candradikusuma; Erma Sulistyaningsih; Josef Sem Berth Tuda; Umar Zein
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Co-Authors Agustina Tri Endharti Al Munawir Alami, Eqiel Navadz Akthar Aldi Cahya Muhammad Alfi Kamalia Aliyatul Farida Anas Bakhtiar Diyansah Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Aprilia, Annisa Nadya Ardyansyah, Rizky Robeth Arifin, Mochammad Samsul Ayundasari, Asti Aziz, Ayu Munawaroh Bagus Hermansyah Burhan, Niniek Cholis Abrori, Cholis Cicih Komariah Denaneer Rahmadatu Devita Prima Nurmasari Dewantoro, Dicky Didi Candradikusuma Didi Candradikusuma Dini Agustina Dion Krismashogi Darmawan Dwi Wahyuni Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Endang Sri Purwanti Wulandari Eny Nurmaida Erfan Efendi Eva Sulawati Arum Fathul Hidayatul Hasanah, Fathul Hidayatul Febrina Sylva Fridayanti, Febrina Sylva Firdha Novitasari Fitri Dika Maharani FX Ady Soesetijo, FX Ady Ghani Silahudin Hadi Prayitno Heni Fatmawati Hidayat Sujuti I Dewa Ayu Ratna Dewanti I Ketut Suada Ida Srisurani Wiji Astuti Ika Rahmawati Sutejo Intan Rachmawati Irawan Fajar Kusuma Istinaroh, Nurul Iza Billa Fahmi Jarot Subandono Jarot Subandono Jauhar Firdaus Josef Sem Berth Tuda Kahfi Karunia Ilahi Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Laeli Kurniasari Laili, Elisa Fadia Laily Rahmawati Loeki Enggar Fitri Luh Putu Ratna Sundari Muhammad Hasan Nabila Putri Anissa Ngurah Agung Reza Satria Nugraha Putra Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Burhan Nur Aqmarina Kusumawardani Nurmaida, Eny Parawita Dewanti Pralampita, Pulong Wijang Pungky Setya Arini Putri, Izza Amalia Rahma, Nafisah Hani Asyifah Rina Fitriana Rina Fitriana Ristya Widi Endah Yani Rosita Dewi Salim Salim Samsul Arifin Sari, Leny Yulia Widia Sa’diyah, Nindya Audatus Setyoadji, Winnie Almira Sheilla Rachmania, Sheilla Sillehu, Sahrir Silvi Ahmada Chasya Sinta Ariyani Sri Hernawati Sugiyanta Sugiyanta Sukarti Moeljopawiro Sukarti Moeljopawiro Thomas Loescher Thomas Loescher, Thomas Tri Agus Siswoyo Tristira Urvina Tuda, Josef Sem Berth Umar Zein Warda Ayu Nadira Wayan T. Artama Wayan T. Artama Widhi Dyah Sawitri Wiji Utami Wiwien Sugih Utami Wulandari, Lindi Priyantika Tri Yunita Armiyanti Zahniar, Zahniar