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Journal : Biocelebes

Uji Efek Antibakteri Ekstrak Etanol Herba Seledri (Apium graveolens Linn.) Terhadap Escherichia coli dan Staphylococcus aureus dan Analisis KLT Bioautografi Khildah Khaerati; Ihwan Ihwan
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

This research divided into two stage which are the inhibition growth capacity test using agar diffusion method with the incubator period 1 x 24 jam hours as the first stage and second stage testing the TLC-Bioautographic. Sample made with the concentration 0,5%, 1 %, 2% and 4% w/v. The capacity of growth inhibited showed on Staphylococcus aureus and than. The TLC-Bioautographic test to the chromatogram of fractionation result with Chloroform : Metanol (15 : 6) on Escherichia coli 1 active stains with Rf value 0,32, for Staphylococcus aureus resulted 2 active stains with Rf value in a row 0,88 and 0,32 Key words: Antibacteri, Apium graveolens, bioautographic.
EFEKTIVITAS EKSTRAK DAUN KERSEN (Muntingia calabura L.) TERHADAP PENURUNAN KADAR KOLESTEROL TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus L.) YANG DIINDUKSI PAKAN TINGGI LEMAK Cornelia Ayu Putri; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Muntingia calabura L. leaves contains saponin and flavonoid that can reduce blood cholesterol levels. This study aimed to examine the effect and the effective dose of M. calabura L. leaves extract to decrease total cholesterol levels of white rats induced by high-fat diet. Extract was found by using ethanol 96% in maseration method. Before doing a different treatment , all the subjects were given a high-fat meal for 14 days to increase the blood total cholesterol levels. The subject were twenty five rats divided into 5 groups as follow: negative control (0.5% Na-CMC), positive control (Simvastatin 1.8 mg/200 g BW), ethanol M. calabura L. leaves extract in various concentration (50; 100; 150 mg/kg BW). All rats were given a different oral treatment for 14 days. Rats blood was taken to measure total cholesterol levels by using Nesco Multicheck Tester on the 7th day after acclimatization, the 14th day after high-fat food induced, and the 7th and 14th day after treatments. The data was analized using non parametric Kruskal Wallis and Mann Whitney statistical test at α = 0.05. The results showed that the ethanol extract of M. calabura L. leaves have an effect to decrease blood total cholesterol levels and the most effective dose to decrease total cholesterol levels were 50 mg/kg BW.
EFEKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN SUMAMBU (Hyptis capitata Jacq.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.) YANG DIINDUKSI DENGAN KARAGENAN Mia Audina; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

One of the medical plants that can be used as an anti-inflamation treatment is sumambu leaf (Hyptis capitata Jacq.). Most of people in binangga village, Subdistric of central Parigi, Regency of Parigi Moutong, have been used this sumambu leaf for deducting inflammation, swelling, and painful. The aimed of this research is to know the effectiveness of the anti-inflamation of Sumambu leaf (Hyptis capitata Jacq.) towards white male mouse (Rattus novergicus L.) which has been given carrageenan induction. The extract was made by using a maseration method using 96% ethanol liquid. The test of the effectiveness of anti-inflamation was divided into your treatments which each treatment was consist of five mice which have been given negative control  0.5% CMC-Na suspension ;100 mg/KgBW, 200 mg/KgBW, 300 mg/KgBW of sumambu leaf extract. After 60 minutes, 0.1 ml carregeenan has been injected using subplantar method at the paw of the mice. The inflamation volume measured using  pletysmometer for 6 hours.  The data collection was statistically analyzed by ANOVA (Analysis of variance) one direction and continued to Duncan test. The results of this research  showed that the three doses of sumambu extract which are ES 100 mg/KgBW, 200 mg/KgBW, 300 mg/KgBWhave the effect of anti-inflamation. Moreover, it can be said that the result of this research showed that the dose that has a lower inflamation percent was ES 300 mg/KgBW. Keywords: Anti-inflammation, Hyptis capitata Jacq., Carrageenan, Inflamed Paw Volume.
PENGARUH PEMBERIAN TOPIKAL EKSTRAK ETANOL DAUN PECUT KUDA (Stachytarpheta jamaicensis (L.) Vahl) TERHADAP PENYEMBUHAN LUKA BAKAR DERAJAT II PADA PUNGGUNG KELINCI (Oryctolagus cuniculus) Muhammad Anggriawan; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Pecut kuda (Stachytarpheta jamaicensis (L.) Vahl) is one of plant that has the potentials to heal various diseases, one of them as an alternative in the cure of burns. Pecut kuda leaf has been used hereditary by some people at Layana Wintu, Mantikulore subdistrict, Donggala regency for cure of burns. This research is conducted to find out the effect of topical administration of ethanol extract of pecut kuda leaf towards healing burns second degree on the rabbit’s back (Oryctolagus cuniculus) by using many extract concentration variation which are 1%, 3% and 5% with gel basic as the carrier. Testing was conducted on 5 rabbits, each rabbit was induced on his back by 5 burns for five treatments with burns diameter is 2 cm . Burns that has been induced was given each treatments which are negative control (gel basic without extract), ethanol extract of pecut kuda leaf in basic gel with each concentration 1%, 3% and 5%. Positive control used bioplacenton gel. Later, the burns diameter was measured on certain day during 28 days. Data that are achieved was  statistically analyzed  with  one way anova method and is continued by testing post hoc duncan with 95% confidance level. The reasult of this research shows every extract ethanol concentration variation of pecut kuda leaf have activity to heal burns. Extract ethanol concentration of pecut kuda leaf which is efective in healing burns is 3% concentration.Keywords: Gel, (Stachytarpheta jamaicensis (L.) Vahl), Burns, Oryctolagus cuniculus
AKTIVITAS EKSTRAK KULIT BATANG LANGSAT (Lansium parasiticum (Osbeck) Sahni & Bennet) TERHADAP KELARUTAN KALSIUM BATU GINJAL SECARA in vitro Nur Ramadhani; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The kidney stone is a stone which is found in the kidney or other parts of urinary tract. The most frequently type of stone encountered are composed of calcium crystals. As much as 60% content of kidney stones consist of calcium oxalate, phosphate, carbonate, uric acid, magnesium, and ammonium. Langsat (Lansium parasiticum (Osbeck) Sahni & Bennet) is a plant that may dissolve kidney stones and smooth urination, particularly its bark. This study aims to determine the activity as well as the greatest concentration of Langsat bark ethanol extract in dissolving dissolves calcium of kidney stones conducted in vitro. The ethanol extract was obtained by maceration method using 96% ethanol followed by phytochemical screening test and thin layer chromatograpy (TLC) profiling of the extract. Chromatogram result shows that the extract contains flavonoids. The research methodology conducted was the kidney stone powder dissolution in the ethanol extract of Langsat bark after being destructed with concentrations of respectively 0.5%, 1% and 1.5% then incubated in 37ºC temperature for 3 hours. The solubility of calcium was assayedby measuring the calcium (Ca) levelsat the beginning and at after being incubated with the kidney stone.The positive control used was Calcusol® and the negative onewas aquadest. The dissolved calcium levels were measured by using Atomic Absorption Spectrophotometer at  λ = 422.7 nm. The research results show that Langsat Bark ethanol extract has the activity in dissolving the calcium of kidney stones in vitro of which concentration with greatestactivity of calcium dissolution is 1.5% w/v. Keywords: Kidney Stone, Langsat, Lansium parasiticum, Calcium.
EFEK ANTIPIRETIK EKSTRAK DAUN CABE RAWIT (Capsicum annum L) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI VAKSIN Difteri Pertusis Tetanus Inggrid Yuliana; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 3 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Leaves cayenne pepper (Capsicum annuum L) is a plant that empirically has been used as a fever-lowering. This study aims to determine the effect of the antipyretic effect of leaf extract of cayenne pepper and determine the effective dose as an antipyretic. Tests carried out using fever inducers ie vaccine DPT (Diphtheria Tetanus Pertussis) 0.2 ml. Tests the antipyretic effect is divided into six treatment groups. The first group was given Na-CMC 1% as the normal control group were given a 2nd Na-CMC and DPT vaccine induced a 0.2 ml as a negative control group all 3 were given paracetamol as a positive control, a group of 4th, 5th and all six were given the leaf extract of cayenne pepper with a dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Rectal temperature measurement is carried out for 6 hours with intervals of 30 minutes. Temperature measurement data were statistically analyzed using oneway ANOVA test. Antipyretic activity test results showed that the leaf extract of cayenne pepper has antipyretic activity in male rats and the dose that effectively lower rectal temperature of rat is 300 mg/kg BW.