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All Journal Microbiology Indonesia Indonesian Journal of Pharmaceutical Science and Technology BIOMA : Jurnal Biologi Makassar Journal of Tropical Crop Science Menara Perkebunan Jurnal Farmamedika (Pharmamedica Journal) JPPIPA (Jurnal Penelitian Pendidikan IPA) Pekemas IPI Garut Current Research on Biosciences and Biotechnology Jurnal Life Science Samakta: Jurnal Pengabdian Kepada Masyarakat
TATI KRISTIANTI
Institut Pendidikan Indonesia Garut
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Diversity of Culturable Bacterial in Various Parts of Luwak’s (Paradoxurus hermaprodithus javanica) Gastrointestinal Tract SONY SUHANDONO; HERI SETIADI; TATI KRISTIANTI; ALI BUDHI KUSUMA; ANDINI WARIH WEDARINGTYAS; DEMI TRISTAN DJAJADI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.379 KB) | DOI: 10.5454/mi.10.2.4

Abstract

Luwak coffee is a highly-priced coffee produced exclusively by the palm civet or luwak (Paradoxurus hermaphrodites ssp.). The purpose of this study was to determine the diversity of culturable bacteria in the gastro intestinal tract of luwak. The bacterial isolates were phenotypically characterized by their morphology and molecularly by analysis of their1,500bp 16s rDNA sequence. The results showed that Enterobacter cloacae and Lactobacillus brevis were found all over luwak’s digestive tract. Enterobacter cloacae was the most common species. The most diverse bacterial population was found in small intestine. Seven bacterial generawere successfully identified from the small intestine and colon, compared to only five genera found in the stomach.
Construction and Expression of Single Recombinant Peptide Surfactant for EOR Application CUT NANDA SARI; USMAN USMAN; RIESA KW ROHMAT; LENI HERLINA; KEN SAWITRI SULIANDRI; ONIE KRISTIAWAN; DWIYANTARI DWIYANTARI; TATI KRISTIANTI; SONY SUHANDONO
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1420.163 KB) | DOI: 10.5454/mi.11.1.5

Abstract

Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually did not degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). PAGE analysis shows that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proves that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).
The transformation of dbr2 and p19 Genes into Artemisia annua L. by Agrobacterium tumefaciens Mediation: Metabolites Analysis Sy, Khairunnisa; Kristianti, Tati; Suhandono, Sony; Elfahmi, Elfahmi
Indonesian Journal of Pharmaceutical Science and Technology 2024: Suppl. 6, no. 3 (The 3rd Mandala Waluya International Conference on Pharmaceutical Science and
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v6i3.54535

Abstract

The low level of Artemisinin in Artemisia annua L. is associated with its biosynthetic pathway. Genetic engineering offers a potential solution to increase its metabolites. The research objectives were to transform the double bond reductase (dbr2), one of the critical genes in the biosynthesis of Artemisinin, and p19, an anti-silencing gene, into A. annua L. by the mediation of Agrobacterium tumefaciens and assess their impact on metabolites production. The dbr2 and p19 genes were verified by analyzing PCR products. Plasmids pCAMBIA1303-DBR2-P19 were transformed into A. tumefaciens and subsequently introduced into the leaves from tissue culture of A. annua L, using vacuum infiltration and assessed by GUS assay. UPLC-ESI-MS/MS measured artemisinic acid (AA), dihydroartemisinic acid (DHAA), and Artemisinin contents. The transformation in A. tumefaciens was successful using a freeze-thaw method. The tissue culture of A. annua L. has been infected by A. tumefaciens using vacuum infiltration. Based on the GUS histochemical assay, the gene has been successfully inserted in the leaves with an efficiency of 48.2%. The result from UPLC-ESI-MS/MS showed that the level of Artemisinin in the transformation sample with and without pCAMBIA-dbr2-p19 was detected but not quantifiable while in wild-type leaves were quantified at 0.008% in fresh weight. The AA and DHAA were not detectable but only in the wild type. The transformation was successful, but the quantification of DHAA and AA was unsuccessful because of the low quantity in the samples.
ANALISIS PROFIL BAKTERI SEDIMEN SUNGAI CIWALEN GARUT Kristianti, Tati; Amalia, Lida
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 1 (2025): Bioma : Januari - Juni 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Kabupaten Garut merupakan daerah yang terkenal sebagai penghasil produk kulit terkemuka di Indonesia. Salah satu dampak dari industri kerajinan kulit ini adalah limbah. Sungai Ciwalen yang terletak di Kecamatan Garut Kota, Kabupaten Garut, Jawa Barat, merupakan salah satu sungai yang dialiri oleh buangan industri penyamakan kulit. Akibatnya air sungai berubah warna menjadi abu-abu dan berbau. Penelitian ini bertujuan untuk mengetahui profile bakteri sedimen sungai Ciwalen dengan menggunakan metode Next-Generation Sequencing (NGS). Hasil analisis NGS menunjukkan urutan bakteri yang diklasifikasikan secara taksonomi menjadi 84.661 bakteri (hampir 100%), 0,001% filum archaeal, dan 0,1% lainnya. Proteobacteria (43279), Bacteriodota (20640), Firmicutes (11813), Acidobacteria (3287), Spirochaetia (1460), Actinobacteria (570), Chloroflexi (469) dan sisanya belum diketahui. Proteobacteria ditemukan lebih dari 50%, yang terbagi menjadi Betaproteobacteria (74%), Gammaproteobacteria (12%), Deltaproteobacteria (10%), Alphaproteobacteria (3%) dan Epsilonprobactria (1%). Bakteriodota didominasi oleh Bactrioidia (82%). Clostridia (66%) dominan di Firmicutes. Holophagae dominan pada Acinobacteria. Spirochaetales dominan di Spirochaetia. Actinomycetia dominan pada Actinobacteria. Klorofleksi ditemukan memiliki lebih dari 88% dari Anaerolineae. Profil bakteri beserta presentasi keberadaannya pada sampel sedimen ini menunjukkan adanya logam berat limbah penyamakan kulit di sungai Ciwalen. Kehadiran logam berat tersebut menjadi habitat yang cocok bagi beberapa bakteri. Beberapa bakteri memiliki beberapa mekanisme untuk melawan dan meremediasi logam berat agar dapat bertahan hidup. Data penelitian ini menunjukkan bahwa Fe>Cr>Pb>NH3-N>Zn>Cu merupakan logam berat dengan konsentrasi tinggi yang terdapat di Sungai Ciwalen. Penelitian ini diharapkan dapat menjadi bahan pertimbangan bagi berbagai pihak yang terkait serta pengambil kebijakan lingkungan.
Isolation and characterization of Dehydrin promoter region from sugarcane (Saccharum officinarum L.) Hayati MINARSIH; Sonny SUHANDONO; Anissa K FUADI; Tati KRISTIANTI; Riza A PUTRANTO; Deden SUKMAJDAYA; . SUSTIPRIJATNO
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.363

Abstract

The development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). While plant tissues are subjected to drought stress (dehydration), DHN protein is accumulated to high content throughout all vegetative or generative tissues. The research aimed to isolate and characterize the DHN promoter from sugarcane that can be used as transformation material in generating drought tolerant sugarcane. Specific primers for DHN promoter amplification were designed and DHN promoter region was successfully isolated by PCR cloning method. Two putative promoter sequences were identified namely Pr-1DHNSo and Pr-2DHNSo. In silicoanalyses were carried out and cis-regulatory elements motifs that play a role in adaptation on abiotic stress as well as biotic stress including ABRE, MBS, CGTCA-motif, TGACG-motif, GARE-motif, P-box TCA-element and Box-W1 were identified. The promoter Pr-1DHNSo was then cloned into pBI121 expression vector by Overlap Extention PCR (OE-PCR) for further characterization. Functional test of the promoter construct pBI- Pr-1DHNSo was conducted through Agrobacterium transformation into sugarcane calli. GUS assay and PCR analysis showed that the DHN promoter was transformed and expressed in the sugarcane calli.
Pelatihan Modul Ajar untuk Memperkuat PCK Guru Biologi: Praktik Baik dari MGMP Kabupaten Garut Rifaatul Muthmainnah; Diah Ika Putri; Tati Kristianti; Suhara Aris
Samakta: Jurnal Pengabdian Kepada Masyarakat Vol. 2 No. 2 (2025): Samakta: Jurnal Pengabdian Kepada Masyarakat
Publisher : CV. Dharma Samakta Edukhatulistiwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61142/samakta.v2i2.237

Abstract

Penguatan Pedagogical Content Knowledge (PCK) menjadi krusial dalam implementasi Kurikulum Merdeka. Kegiatan pengabdian ini bertujuan membekali guru Biologi MGMP Kabupaten Garut dengan keterampilan menyusun modul ajar yang sistematis, kontekstual, dan berbasis karakter. Pelatihan dilaksanakan dalam bentuk workshop dan pendampingan daring, melibatkan 42 guru. Materi mencakup penyusunan modul berbasis Capaian Pembelajaran (CP), Tujuan Pembelajaran (TP), karakteristik peserta didik, dan asesmen. Evaluasi dilakukan melalui angket tertutup dan terbuka. Hasil menunjukkan peningkatan pemahaman guru terhadap penyusunan modul ajar, integrasi profil pelajar Pancasila, dan pemilihan asesmen. Namun, aspek TP dan asesmen memerlukan penguatan lanjutan. Kegiatan ini menegaskan pentingnya kolaborasi antara perguruan tinggi dan MGMP dalam pengembangan profesional guru.
Efektivitas Metode Pembelajaran Hands-on Minds-on Activity terhadap Hasil Belajar Siswa pada Materi Sistem Indera Dodo, Indra; Tati Kristianti; Bunga Rahma Mutia; Rifaatul Muthmainnah
Jurnal Life Science : Jurnal Pendidikan dan Ilmu Pengetahuan Alam Vol. 8 No. 1 (2026)
Publisher : IPI Garut Press

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Abstract

Pendidikan abad ke-21 menuntut siswa memiliki keterampilan berpikir kritis, kolaboratif, dan kreatif, namun dalam praktiknya masih banyak siswa yang mengalami kesulitan dalam memahami konsep-konsep biologi yang kompleks seperti sistem indera manusia. Hal ini ditingkatkan menjadi lebih parah dengan penggunaan metode pembelajaran konvensional yang membuat siswa kurang aktif saat proses pembelajaran. Salah satu solusi yang ditawarkan adalah penerapan metode pembelajaran hands on minds on activity yang menekankan agar siswa aktif, mandiri, dan terlibat langsung dalam proses belajar. Penelitian ini bertujuan untuk mengetahui efektivitas metode pembelajaran hands on minds on activity dalam meningkatkan hasil belajar siswa pada materi sistem indera manusia di kelas XI SMA Negeri 11 Garut. Penelitian menggunakan pendekatan kuantitatif dengan desain pra experiment tipe one-group pretest-posstest terhadap 40 siswa, dengan pengumpulan data melalui tes pretest dan posttest yang dianalisis menggunakan uji gain ternormalisasi dan uji-t. Hasil penelitian menunjukkan signifikan hasil belajar siswa, dengan rata-rata gain berada pada kategori sedang dan tingkat ketuntasan mencapai 92,5%, melampaui ambang batas efektivitas sebesar 75%. Peningkatan ini dicapai melalui proses pembelajaran yang aktif dan sistematis, dimana metode pembelajaran hands on minds on activity memfasilitasi keterlibatan langsung siswa dalam kegiatan belajar. Dengan demikian, metode pembelajaran hands on minds on activity efektif diterapkan untuk meningkatkan hasil belajar siswa pada materi sistem indera manusia secara aktif dan bermakna.
Co-Authors . SUSTIPRIJATNO Agus Chahyadi Amalia, Lida ANDINI WARIH WEDARINGTYAS Anissa K FUADI Ari Widodo Ari Widodo Bunga Rahma Mutia Chevi Ardiana CUT NANDA SARI Deden SUKMAJDAYA DEMI TRISTAN DJAJADI DEWI AYU KENCANA UNGU Dewi Hernawati Diah Ika Putri Dodo, Indra DWIYANTARI DWIYANTARI DWIYANTARI, DWIYANTARI DWIYANTARI, DWIYANTARI EDHYANA SAHIRATMADJA Elfahmi Elfahmi, Elfahmi Hayati MINARSIH HERI SETIADI HERLINA, LENI HERLINA, LENI Herman Susanto I Nyoman Pugeg Aryantha Indra Dodo Saputra Iskandar, Hayati Minarsih KEN SAWITRI SULIANDRI Khairunnisa Sy Khairunnisa W.R., Riesa KRISTIAWAN, ONIE KRISTIAWAN, ONIE KUSUMA, ALI BUDHI Laode M.R. Al Muqarrabun LENI HERLINA Leni Sri Mulyani Lestary, Refiana Lida Amalia Marlin Megalestin Raunsai Mose, Windi Nina Imaniar ONIE KRISTIAWAN Pambudi, Jembar Pasarai, Usman Pasarai, Usman PUTRANTO, Riza Arief Refiana Lestary Riesa Khairunnisa W.R. RIESA KW ROHMAT Rifaatul Muthmainnah Riza A PUTRANTO Rohmat, Riesa K. W Rohmat, Riesa K. W SARI, CUT NANDA Sari, Cut Nanda Sonny SUHANDONO SONY SUHANDONO SONY SUHANDONO Sony Suhandono Sony Suhandono Sony Suhandono Sri Mulyaningsih Suhandono, Sonny Suhara Aris Suliandari, Ken Sawitri Suliandari, Ken Sawitri Sustiprijatno Sustiprijatno, Sustiprijatno syafrizal Syafrizal Syafrizal Usman Usman Usman