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Biotic analysis of the implementation of standard operating procedures (SOP) in banana tissue culture in the UPTD seed laboratory Deswita Maharani Br Sembiring; Ema Tesalonika Simangunsong; Mhd Yusuf Nasution; Khairiza Lubis; Widya Arwita
EduMatika: Jurnal MIPA Vol. 6 No. 1 (2026): EduMatika: Jurnal MIPA
Publisher : Lembaga Riset Mutiara Akbar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56495/emju.v6i1.1567

Abstract

Banana tissue culture is an aseptic plant propagation technique that plays a crucial role in producing superior, uniform, and disease-free seedlings. The success of this technique is greatly influenced by the implementation of Standard Operating Procedures (SOPs) and the application of bioethical principles in laboratory activities. This study aims to analyze the implementation of banana tissue culture SOPs and their compliance with bioethical principles in the Horticultural Parent Seed UPTD Laboratory. The study used a qualitative approach with descriptive-analytical methods. Data were obtained through direct observation, interviews, and documentation during laboratory activities. The results showed that the implementation of SOPs has not been optimal, indicated by the non-mandatory use of personal protective equipment (PPE) during media preparation, the transfer of equipment through open areas that have the potential to cause contamination, and limited laboratory facilities, especially water availability. From a bioethical perspective, these conditions indicate a suboptimal implementation of the principles of occupational safety, scientific responsibility, and laboratory sterilization. Therefore, increased supervision, disciplined implementation of SOPs, and improvements to laboratory facilities are needed to support safe, sterile, and bioethical tissue culture activities.
Application of avocado (Persea Americana) grafting in a bioethical framework at the Johor Building Horticultural Parent Seed UPTD Agnes Banjarnahor; Desi Kristina Hutagalung; Linda Lestari Sianipar; Khairiza Lubis; Widya Arwita
EduMatika: Jurnal MIPA Vol. 6 No. 1 (2026): EduMatika: Jurnal MIPA
Publisher : Lembaga Riset Mutiara Akbar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56495/emju.v6i1.1569

Abstract

This study aims to analyze the implementation of Standard Operating Procedures (SOPs) in avocado grafting practices and review their implementation from a bioethical perspective at the UPTD Benih Induk Hortikultura Gedung Johor, Medan City. The study used a descriptive qualitative approach with data collection techniques through field observations, interviews with nursery technical staff, and activity documentation. The results showed that the grafting process was carried out through several main stages, namely rootstock preparation, scion selection from superior parent plants, the cambium tissue grafting process, and the post-grafting maintenance stage. The implementation of SOPs in these activities plays an important role in maintaining the consistency of nursery techniques and increasing the success rate of grafting. From a bioethical perspective, the implementation of SOPs reflects professional responsibility in biological resource management, particularly in maintaining seed quality, the sustainability of superior varieties, and the efficiency of horticultural crop production. Standardized grafting practices are not only oriented towards increasing productivity but also demonstrate efforts to manage biological resources responsibly and sustainably.
Analysis of the application of bioethical principles in fertilizing horticultural plants at the Medan Johor horticulturalparent seed uptd Christnamy Ayu Pertiwi Sihole; Lastria Oktaviana Simbolon; Sulvin Kurnia Gulo; Khairiza Lubis; Nurbaity Situmorang
EduMatika: Jurnal MIPA Vol. 6 No. 1 (2026): EduMatika: Jurnal MIPA
Publisher : Lembaga Riset Mutiara Akbar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56495/emju.v6i1.1570

Abstract

The horticultural sector plays a strategic role in food security, but uncontrolled use of chemical fertilizers can threaten ecological sustainability. This study aims to analyze the application of bioethical principles, namely beneficence, non-maleficence, justice, and responsibility, in horticultural plant fertilization practices at the Medan Johor Horticultural Seed Main Plant (UPTD). This study used a descriptive qualitative method with a case study approach. Data were collected through field observations, structured interviews, and Standard Operating Procedure (SOP) documentation. The results show that the UPTD has attempted to integrate bioethical principles through balanced fertilization management between organic and inorganic fertilizers, equitable seed distribution to the community, and compliance with environmental regulations. However, this implementation still faces several challenges, such as limited knowledge of bioethics among practitioners and financial constraints in adopting environmentally friendly technologies. This study recommends increasing human resource capacity through agricultural ethics training and strengthening environmental-based internal regulations to ensure sustainable seed quality.
Multifunction healthy pomade balm pump with Batak spice extract Clarisa Yesilia Br Simanjuntak; Putri Jasrina; Angeleva Sinaga; Gracia Napare Sihombing; Ali Ahmatul Umri Hasibuan; Khairiza Lubis
EduMatika: Jurnal MIPA Vol. 4 No. 3 (2024): EduMatika: Jurnal MIPA
Publisher : Lembaga Riset Mutiara Akbar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56495/emju.v4i3.1712

Abstract

Issues concerning the lips, hair, and skin such as chapped lips, dull and brittle hair, and rough, scaly skin frequently arise from adverse environmental factors and prolonged exposure to harmful synthetic cosmetic ingredients. This study investigates the formulation potential of POMPA Multifunction Healthy Pomade Balm, an innovative product combining lip balm, hair pomade, and skin moisturizer into a single compact packaging utilizing natural spices indigenous to North Sumatra. A systematic literature review was conducted by synthesizing 10 peer-reviewed articles (2017–2023) retrieved from the Google Scholar database regarding natural cosmetics. The literature review indicates that the synergy of fragrant pandan leaves, patchouli oil, citrus, and olive oil provides an abundant source of natural antioxidants, flavonoids, saponins, vitamin C, and essential fatty acids. These bioactive constituents effectively restore lip moisture, strengthen hair roots while preventing dandruff, and maintain cutaneous hydration. Consequently, POMPA represents a viable, sustainable, and multifunctional herbal cosmetic innovation for holistic personal care.