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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Penelitian Saintek ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Teknosains: Media Informasi Sains dan Teknologi Prosiding KPSDA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Energi Baru dan Terbarukan AGRIC Journal of Bioresources and Environmental Sciences Jurnal Bioteknologi & Biosains Indonesia
Endang Kusdiyantini
Departemen Biologi Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Potensi Rizobakteri Pembentuk Endospora dari Brokoli (Brassica oleracea var. Italica) sebagai Agen Biokontrol Ralstonia solanacearum serta Biofertilizer Fauzaan, Muhammad Faishal; Wijanarka, Wijanarka; Kusdiyantini, Endang; Budiharjo, Anto; Ferniah, Rejeki Siti
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jis.%v.%i.%Y.210-226

Abstract

Penyakit Layu Bakteri merupakan penyakit tular tanah yang menyerang tanaman hortikultura yang disebabkan oleh bakteri fitopatogen Ralstonia solanacearum. Komunitas bakteri tanah yang menghuni rizosfer tanaman diketahui memiliki potensi yang baik dalam mendukung pertumbuhan maupun melindungi tanaman dari serangan patogen. Bakteri pembentuk endospora dipilih karena memiliki toleransi yang cukup bagus apabila dihadapkan dengan lingkungan yang tidak cocok bagi pertumbuhannya. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi isolat rizobakteri pembentuk endospora potensial sebagai agen biokontrol melawan R. solanacearum dari rizosfer brokoli sehat di lahan pertanian organik desa Kopeng Kabupaten Semarang serta mengetahui potensinya sebagai agen biofertilizer. Isolasi rizobakteri pembentuk endospora menggunakan metode spread plate; purifikasi isolat rizobakteri menggunakan metode streak plate, karakterisasi dengan uji pewarnaan Gram dan uji pewarnaan endospora, uji antibakteri melawan R. solanacearum menggunakan metode Kirby-Bauer; identifikasi molekuler dengan gen 16S rRNA; konstruksi pohon filogenetik dan uji potensi biofertilizer berupa uji produksi IAA menggunakan reagen Salkowski, uji pelarutan fosfat menggunakan media Pikovskaya dan uji fiksasi nitrogen menggunakan media Nitrogen-free Bromothymol-blue. Hasil penelitian diperoleh 18 isolat rizobakteri pembentuk endospora; isolat RB5 memiliki potensi antibakteri dengan daya hambat kategori sedang (6 mm) dan teridentifikasi sebagai Bacillus subtilis strain FP.PT.1.1-CTCRI dengan nilai similaritas sebesar 98,10% serta mampu memroduksi IAA dan melarutkan fosfat tetapi tidak mampu memfiksasi nitrogen secara kualitatif.
Produksi dan Ekstraksi Pigmen Anka Monascus purpureus pada Beras IR42 Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.162-165

Abstract

Monascus merupakan kapang filamentous penghasil pigmen yang telah lama digunakan sebagai pewarna alami pangan, terutama M. purpureus dan M. ruber. Anka merupakan kultur padat dari M. purpureus dengan media beras. Tujuan penelitian ini untuk analisis pigmen merah, oranye dan kuning dari Anka M. purpureus. Beras IR42 digunakan untuk pembuatan Anka yang diinkubasi selama 30 menit. Ekstraksi pigmen menggunakan solven metanol dan diukur dengan spektrofotometer pada panjang gelombang 500 nm untuk pigmen merah, 470 nm untuk oranye dan 390 nm untuk pigmen kuning. Anka yang dihasilkan berwana merah yang menunjukkan adanya pertumbuhan M purpureus. Konsentrasi pigmen tertinggi diperoleh dari pigmen kuning sebesar 1.121 CVU/g, sedangkan pigmen merah sebesar 1.049 CVU/g dan yang paling rendah adalah pigmen oranye sebesar 837 CVU/g.
ANALYSIS OF BIOMETHANE PRODUCTION POTENTIAL AND MITIGATION OF BIOMETHANE EMISSIONS FROM PIG FARMS IN NORTH SULAWESI PROVINCE Manullang, Efrando; Sutaryo; Kusdiyantini, Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2803

Abstract

The potential for biomethane production from livestock manure as renewable energy has good prospects, considering that the number of pigs in North Sulawesi Province increases yearly. Therefore, it is necessary to analyze the potential for biomethane production, replacing the use of LPG, and mitigate biomethane emissions from pig farms. The results showed that from 2015-2021, the most significant biomethane production was in 2021, amounting to 7,887 tons of CH4/year in the Minahasa Regency area, 2,496 tons of CH4/year (31.65%) in Tomohon City, 805 tons of CH4/year (10.20%) in South Minahasa Regency, 779 tons of CH4/year (9.88%), and in other areas 3,807 (48.27%). Biomethane can replace 8.59 million kg of Liquefied Petroleum Gas (LPG). The results of mitigating biomethane emissions in 2021 reached 34.22 Gg CO2eq, and using biomethane to replace LPG reached 11.59 Gg CO2eq. Total greenhouse gas emissions can be reduced to 45.81 Gg CO2eq. Biomethane from pig manure has promising potential as a new renewable energy and requires further investigation.
Cellulose Hydrolysis of Mask Waste Using Aspergillus niger and Eco-Friendly Microwave Pretreatment Gilbran, Adam; Nafilah, Syahraini; Layalia, Afina Rista; Arsyad, Wifqul Muna; Darmawan, Andi; Setiawan, Risqi Prastianto; Irviandi, Risnu; Kusdiyantini, Endang; Nurauliyaa, Aida Habibah; Anda, Martin; Sasongko, Nugroho Adi; Wahyono, Yoyon
Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Vol 22, No 3 (2025): November 2025
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/presipitasi.v22i3.993-1001

Abstract

The management of used medical mask waste has become a significant issue due to the increased volume of waste during and after the pandemic. Medical mask waste contains cellulose compounds that can be converted into derivatives such as glucose, which are then processed into bioethanol as an alternative energy source. This study aims to hydrolyse medical mask waste using cellulase enzymes from Aspergillus Niger to produce glucose. The cellulase enzyme composition was varied (5 ml, 15 ml, and 25 ml) to determine the optimal hydrolysis conditions. The glucose produced was measured using DNS reagent assay with spectrophotometry at a wavelength of 540 nm. The highest amount of glucose was obtained under optimal conditions with 25 ml of cellulase enzyme after 48 hours of hydrolysis, amounting to 88.16 ppm. Subsequently, the glucose from hydrolysis was fermented using Saccharomyces cerevisiae, and the fermentation product was analysed for ethanol concentration using GC-FID. The products of fermentation ware 0.017% ethanol concentration from mask waste fermentation. Hydrolysis is an environmentally friendly alternative solution for handling mask waste.
Model and Simulation of Solar-Powered PEM Water Electrolysis for Green Hydrogen and Environmental Assessment in the IKN Siahaan, Parluhutan; Sulistyo, Sulistyo; Kusdiyantini, Endang
Journal of Bioresources and Environmental Sciences Accepted Issue
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61435/jbes.2025.19985

Abstract

Energy demand in Indonesia continue to rise inline with population and economic growth. Using Jakarta city of Indonesia as representative case, energy consumption in the  transportation sector has risen significantly from year to year. This escalation contributes to deteriorating air quality and poses adverse impacts on public health. To prevent similar condition in the new capital city (IKN), this study examines green hidrogen production to support Fuel Cell Electrical Vehicle (FCEV)-based transportation. The objective of this research is to evaluate the technical,  economic feasibility and environmental benefit of a solar pv-power driven Proton Exchange Membrane Water Electrolizer (PEMWE) system for large-scale hydrogen generation in IKN. A dynamic PEMWE model was developed and simulated using Matlab/Simulink/Simscape under operating temperature of 60 °C, 80 °C and 100 °C at a current density of 1,2 A/cm2. Key performance indicators evaluated include membrane water diffusion flux, electro-osmotic drag, hydrogen production rate and system efficiency.  Result shown that operation 80 °C achieves the optimal performance. Scaled-up calculation indicate a hydrogen output of 3,006.62 kg/day with an electricity demand of 143,617 kWh, resulting in specific energy consumption of 47,9 kWh/kg outperforming the commercial PEMWE range 51-55 kWh/kg. This production capacity can fuel approximately 1,500 FCEVs corresponding to a potential CO2 emission reduction of 13,112.7 kg CO2-eq/day. Economic analysis using the Levelized Cost of Hydrogen (LCOH), with a 6% WACC and 20-years project life, yields an annualized CAPEX of roughly MUSD 31 and electricity dominated OPEX of KUSD 577 per year. The resulting LCOH od USD 3.0/kg H2 aligns with projected 2030 green hydrogen cost target. In conclusion, Solar pv-powered PEMWE development in IKN is demonstrates holistic feasibility in term of technical viable, economically competitive and environment impactful. 
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anda, Martin Andi Darmawan Anggraini, Ika Anggraini, Ika Anggraini, Ika Anggraini Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Arsyad, Wifqul Muna Atit Kanti Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Chatarina Umbul Wahyuni Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Eka, Prastya Muhammad Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Fauzaan, Muhammad Faishal Fransenda, Auxensius Rexer Gilbran, Adam Ginting, Ledy Ginting, R Cinta Badia H Hadiyanto Hadi Endrawati Hapzi Ali Herida, Azalia Puspa Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Indah Sulistyarini Irviandi, Risnu Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kumudasmoro, Bagas Mukti Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Layalia, Afina Rista Manullang, Efrando Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Nafilah, Syahraini Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nugroho Sasongko Jati, Nugroho Sasongko Nunung Eni Elawati Nunung Eni Elawati Nurauliyaa, Aida Habibah Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurmila, Ika Oktavia Pardede, Lasria Parluhutan Siahaan Pratama, Novi Alvita Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Putri, Adde Lolita Octavia Rejeki Siti Ferniah Rida Yuliana, Rida Safitri, Linda Saniha Adini, Saniha Sarjana Parman Setiawan Wicaksono, Setiawan Setiawan, Risqi Prastianto Siregar, Mangasa Tua Pandapotan Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Sulistyo Sulistyo Suryaningsih, Vivi Sutaryo TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wahyono, Yoyon Wijanarka Wijanarka Wijanarka, W Yeti Darmayati